Purpose: This study is an explorative survey to examine emotional labor, stress, and premenstrual syndrome among hospital nurses and to examine relationships among them. Methods: Data were collected from 228 nurses working at hospitals using structured questionnaires from September to October, 2014. Data were analyzed using SPSS 21.0 by frequency, descriptive statistics, t-test, one-way ANOVA, and Pearson's correlation coefficient. Results: Score of emotional labor was different by work time per week (F=4.03, p=.019), and menstrual amount (F=5.18, p=.006). Level of stress was different by marital status (t=2.29, p=.023), pattern of work (t=-3.63, p<.001), work time per week (F=3.39, p=.035), regularity of menstrual cycle (t=-4.20, p<.001), and exercise frequency (F=4.28, p=.015). Scores of premenstrual syndrome were different by regularity of menstrual cycle (t=-3.18, p=.002), and menstrual amount (F=5.88, p=.003). Emotional labor was related with perceived stress (r=.40, p<.001) and premenstrual syndrome (r=.23, p<.001). Also, perceived stress was related with premenstrual syndrome (r=.33, p<.001). Conclusion: Nurses' emotional labor, stress, and premenstrual syndrome were higher than the average. Emotional labor was correlated with stress and premenstrual syndrome, premenstrual syndrome with stress. This study shows that it is necessary to understand these relationships and to search for nursing intervention to ease emotional labor, stress, and premenstrual syndrome.