• Journal of Bio-Environment Control
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• v.30 no.1
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• pp.77-84
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• 2021

This study attempted to find a way to maintain the quality of mixing baby wild leaf vegetables with existing baby leaf vegetables in various ratios. The crops for mixing baby leaf vegetables were Peucedanum japoincum Thunberg and Ligularia stenocephala, as wild vegetables, and red romaine, which is widely used in young leafy vegetables. The mixing ratio of red romaine and wild vegetables was red romaine 0: mantilla oil 5: L. stenocephala ratio 5 (R0: P5: L5), red romaine 3.3: P. japoincum 3.3: L. stenocephala ratio 3.3 (R3.3: P3.3: L3.3), red romaine 5: P. japoincum 2.5: L. stenocephala 2.5 (R5: P2.5: L2.5), red romaine 8: P. japoincum 1: L. stenocephala 1 (R8: P1: L1), red romaine 10: P. japoincum 0: L. stenocephala 0 (R10: P0: L0). All treatments were packaged in OTR (oxygen transmittance) 10,000 cc m-2·day-1·atm-1 film and stored for 27 days at 2℃/85% RH conditions. Fresh weight, carbon dioxide, oxygen, and ethylene concentrations of the baby leaf packages were examined approximately every 3 days, and visual quality, chlorophyll content, and chromaticity were examined on the 27th day of storage. The oxygen and carbon dioxide concentration in the packages were affected by the respiration rate of the crop. As the mixing ratio of lettuce, which had a low respiration rate, increased, the oxygen concentration in the packages was higher and the carbon dioxide concentration was lower. Oxygen concentration decreased significantly after 15 days, but was remained above 16%, and on the contrary, carbon dioxide concentration was kept at 1-4% until the 15th, and then gradually increased to 2-5% on the 27th day. The concentration of ethylene was maintained at 3-6 µL·L-1 until the end of storage (27th day). Visual quality score measured at the end of storage was slightly less than 3.0, which is the limit of marketability of all treatments. Although there was no significant difference, the chlorophyll content (SPAD) of red romaine and P. japoincum were most similar with an initial value in R8:P1:1 treatment, and L. stenocephala was higher value in R8:P1:L1 and R5:P2.5:L2.5 treatments at the end of storage. The leaf color (L∗, a∗, b∗, chroma) of the three crops at end of storage compared with the heat map showed the least change in the R5:P2.5:L2.5 and R8:P1:L1 treatments at the end of storage. Among them, R8:P1:L1 treatment maintained the highest chlorophyll content, the second lowest ethylene concentration, and adequate carbon dioxide concentration of 2-3%. Therefore, it is judged that the mixed ratio of red romaine 8: P. japoincum 1: L. stenocephala 1 (R8: P1: L1) is most suitable for the mixed package of baby leaf vegetables of these three crops.

• Korean Journal of Plant Tissue Culture
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• v.21 no.3
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• pp.137-143
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• 1994

In an attempt to optimize the in vitro-regeneration conditions necessary for the genetic manipulation of tomato species, we examined several hybrid lines and wild species (peruvianum, pimpinellifolium, glandulosum) of Lycopersicon for. their, different regeneration ability. The basal medium used for callus growth and organogenesis was MSB (MS + B5) supplemented with three combinations of TDZ (Thidiazuron) 0.5mg/L+NAA 0.5mg/L, BA 2.0mg/L+NAA0.05 mg/L, and zeatin 3.0 mg/L + IAA 0.02 mg/L. In the genotype of Lycopenicon grandulosum, combination of TDZ and NAA was more effective in inducing shoot and root differentiation than those of BA and NAA or zeatin and IAA. When all genotypes tested were considered, however combination of zeatin and IAA was shown to be the best in shoot regeneration. Result indicate that callus and organogenesis of Lycopenicon species are dependent upon the hormone types and plant genotypes, but MSB medium with zeatin 3.0 mg/L + IAA 0.02 mg/L maybe appropriate for genotype-independent plant regeneration system of Lycopercicon species. We also tried TDZ as a cytokinin source in tomato tissue culture and found it highly significant in tomato regeneration system.

• Journal of the Korean Chemical Society
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• v.39 no.6
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• pp.434-439
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• 1995

Six-coordinate molybdenum(Ⅴ)-oxo complexes, (R4N)[MoO(NCS)2L](R=CH3, C2H5, n-C4, H9) with S-methyl-3-(2-hydroxy-x-phenyl)methylenedithiocarbazate(L1: x=5-H) and its derivatives (L2:x=5-CH3, L3: x=3-CH3O, L4: x=5,6-C4H4 and L5: x=5-NO2) have been synthesized and the structural, spectral and electrochemical properties of the complexes have been characterized by elemental analysis, molar conductivity, UV-Vis, IR, 1H NMR, and CV (cyclic voltammetry).

• Korean Journal of Plant Tissue Culture
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• v.25 no.1
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• pp.27-31
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• 1998

Immature hybrid embryos of H. hybridum, 'Picottee', 'White Christmas', 'Eldorado', 'Origin', 'Red Lion', 'elstar', 'Crypsy' were cultured on the MS medium supplemented with various concentrations of 2,4-D, NAA, BA and TDZ. Among the treatments, NAA were more effective for the shoot regeneration and bulblet formation than other treatment. Addition of 0.5 ㎎/L NAA was effective for bulblet induction from explant Shoot regeneration was most effective on the medium with 1.0㎎/L NAA and 2.0 ㎎/L TDZ. The addition of 1.0-2.0㎎/L TDZ induced numerous shoots per explant but strongly inhibited root development when compared to 1.0-2.0㎎/L BA. When bulb scale segments of 'Star Van Holland' was incubated, bulblet formation was the most effective on MS medium with 0.5㎎/L NAA.

• Korean Journal of Veterinary Service
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• v.33 no.3
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• pp.223-231
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• 2010

A total of 191 samples collected from the commercial swine farms located in Chungnam province were investigated by PCR to estimate the prevalence of Lawsonia (L.) intracellularis infection. In the group of the pigs with proliferative enteritis, 14 (93.3%) of 15 intestinal samples and 12 (80.0%) of 15 feces were positive in PCR. In contrast, a relatively low positive rate (18.0%, 29 of 161 samples) was determined in the group of normal healthy pigs. The group of pigs over 120 days showed the highest positive rates (26.8%, 15 of 56 samples). In the comparison of the sequences of 210bp for species specific fragments and 301bp for outer membrane protein, the isolates (L1. L2) showed almost 100% identity with the reference L. intracellularis (L08049, USA). For the sequences of partial 16s rDNA, the homologies among the 5 isolates (L1-L5) were 97.4% to 99.3%, and those of 5 sequences (L1-L5) versus 5 overseas reference strains of L. intracellularis ranged from 98.6% to 99.8%. In the comparison of the nucleotide sequences among 5 isolates and other species in Desulfovibrionales showed 82.4 to 99.5% identities. The 5 isolates shared relatively low identities (76.9% to 84.4%) with the species of alpha-proteobacteria. In phylogenetic analysis based on the 16s rDNA sequences, all of the 5 isolates (L1-L5) were located in the same branch with the strains of L. intracellularis that were previously isolated from the pigs in USA and China. Seven strains of Desulfovibrio sp. were clustered in the neighboring branches, whereas alpha and gamma Proteobacteria showed distant relationship with L. intracellularis strains. The present findings suggest that L. intracellularis infection is endemic in the swine farms in the regions, and that the domestic isolates maintained very limited genetic variation.

• The Journal of Churna Manual Medicine for Spine and Nerves
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• v.6 no.2
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• pp.53-60
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• 2011

Objectives : The purpose of this study is to find out the relation between the height of intercrestal line, and HIVD(Herniated Intervertebral Disc)of Lumbar spine. Methods : We investigated 445 cases (208 male, 237 female) of patients who were diagnosed as HIVD of L-spine at either L4/5 or L5/S1 level. We analysed the relation between the height of intercrestal line and the level of HIVD. Results : 1. Among 445 cases, the level of intercrestal line with HIVD of L-spine at the specific location was different between male and female. In male, the intercrestal line is more likely to be located at L4 level, while in female more likely to be located at L5 level. 2. Among 445 cases, L5/S1 HIVD patients(272, 61.1%) were more than L4/5 HIVD patients(173, 38.9%). At L4 body area, L4/5 HIVD patients were more than L5/S1 HIVD patients, however, at L4/5 intervertebral area and L5 body area, L5/S1 HIVD patients were more than L4/5 HIVD patients. (p<0.01) 3. When the line is located at higher level, HIVD of L-spine tends to be occurred at L4/5 level. Contrastly, when the line is located at lower level, HIVD of L-spine tends to be occurred at L5/S1 level. (p<0.01) Conclusions : High intercrestal line leads to L4/5 HIVD, while low intercrestal line leads to L5/S1 HIVD. Possibly, it is caused by different length and thickness of the iliolumbar ligament.

• KSBB Journal
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• v.5 no.4
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• pp.383-390
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• 1990

Alcoholic distillery waste was utilized as dual purposes to produce citric acid and to reduce the amount of waste to be treated. Enzyme and acid hydrolysis of this waste were studied to suggest effective way of present purpose. Enzymatic hydrolysis of this naked barley alcoholic distillery waste by $\alpha$-and $\beta$-amylase gave glucose as 8g/l concentration at $55^{\circ}C$ for 6 hours, which produced 1g/l citric acid and 5.33g/l mycelial. This waste material hydrolyzed with 25% HCl at $120^{\circ}C$ showed 21.5g/l glucose and produced 1.75g/l citric acid with 4.9g/1 mycelial. The glucose concentration was decreased to 3.44g/l by further 2nd acid hydrolysis because the monosugars were decomposed at prolonged hydrolysis conditions. The addition of 3g/l $NH_4NO_3$ increased the mycelial growth but reduced the amount of citric acid formed. The formation of citric acid was increased at low concentration of manganese ion.

• Journal of Life Science
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• v.10 no.3
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• pp.247-253
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• 2000

The effects of acute of marine toxicants on early life of coastal olive flounder were investigated. An increasing order of acute toxicity on embryo- and larva-stages of Paralichtys olivaceus was CdCl2$\mu\textrm{g}$/L and 29 $\mu\textrm{g}$/L, respectively, and those values at larva-stage were estimated 3.5 ng/L, 16.0 nL/L, 10.5 $\mu\textrm{g}$/L and 15.0 $\mu\textrm{g}$/L, respectively.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.04a
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• pp.121.2-122
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• 2003

다슬기분말(PSB)과 그 회분(ASB)으로 제조한 칼슘락테이트(PCaL 및 ACaL)를 0.5%씩 첨가한 반죽의 발효와 빵의 품질 및 저장성에 미치는 영향을 조사하였다. 반죽의 pH는 4.85~4.98로 ACaL.PCaL.대조군의 순으로 나타났다. 반죽의 부피와 빵의 loaf volume index는 대조군이 높았고 ACaL 첨가군이 낮았으며, pH를 5.50으로 조정하여 제조한 반죽의 부피와 빵의 loaf volume은 대조군과 큰 차이를 보이지 않았다. PCaL 및 ACaL을 첨가한 빵의 Ca함량은 29.4~29.7 mg/100 g-f.w로 대조군의 13.0 mg/100 g-f.w에 비하여 높았으며, 첨가군의 미량 무기질로 Mg, Fe, Zn이 0.03~0.98 mg/100 g-f.w 범위로 검출되었다. 빵의 L$^{*}$ 값은 대조군과 실험군의 유의적인 차이가 없었으며, a＊값, b＊값은 PCaL 첨가군이 가장 높아 황갈색을 띄었다. 빵의 hardness, gumminess는 대조군$^{\circ}C$ 실온에 두면서 저장한 결과 대조군은 3일째부터, ACaL 첨가군은 5일째부터, PCaL 첨가군은 6일째부터 곰팡이가 번식하였다.

• Journal of Environmental Health Sciences
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• v.17 no.1
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• pp.74-78
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• 1991

This study was performed to investigate the effect of heavy metals and CN on BOD$_{5}$ measurement. The results were as follows: 1. The BOD$_{5}$ value decreased 20% in Cd(CdCl$_{2}$) 3.0mg/l, Ni(NiCl$_{2}$) 7.0mg/l, Cu(CuCl$_{2}$) 0.5mg/l, Pb($Pb(NO_{3})_{2}$) 9.0mg/l, Cr($K_{2}Cr_{2}O_{7}$) 1.0mg/l respectively. 2. The BOD$_{5}$ value decreased 10% in Zn($Zn(NO_{3})_{2}$) 5 mg/l, Fe(FeCl$_{3}$) 20mg, /l, and 25% in CN(KCN) 5mg/l, and 40% in Hg(HgCl$_{2}$) 0.2rng/l. 3. In Comparion with BOD Rapid Analyzer, the above mentioned heavy metals concentrations have no effect on BOD$_{5}$ value of that analyzer except CN.