• Applied Biological Chemistry
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• v.18 no.4
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• pp.194-202
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• 1975

This study was conducted to study the properties of the water-soluble natural chelating agents from garbage compost and activated sewage sludge responsible for Fe chelation, which is closely associated with the effectiveness in correcting iron chlorosis in plant. The water-soluble fraction of these materials was fractionated by menas of Sephadex gel filtration and the fractions of Fe chehates were traced by radioactive $^{59}Fe$. The fractions were examined by ultraviolet and infrared. spectroscopy and stability constants for Fe. The water-soluble fraction from garbage compost was separated by Sephadex G-25 into approximately four fractions. Most of the added $^{59}Fe$ was associated with fraction I, which appeared at the void volume. Further fractionation by Sephadex G-50 indicated that the molecular weight of water-soluble chelating agents is in the approximate range of 5000 to 10,000. The water-soluble fraction from activated sewage sludge gave six fractions by Sephadex G-25. Most of the added $^{59}Fe$ was found in the fraction I,II, and III, The molecular weights of most chelating agents associated with $^{59}Fe$ appeared to be less than 5,000 and those of fraction I that appeared at the void volume was in the range of 5,000 to 1,000. Discrepancy between radio activity count and UV absorption indicated the heterogeneity of the fractions obtained by Sephadex gel filtration. Ultraviolet absorption spectra of all fractions separated by Sephadex G-25 and containing chelating agents showed no differences. Fraction IV and V of sewage extract showed absorption maxima and shifting similar to nucleic acid components suggesting the presence of decomposition products of nucleic acid. Similarity fraction VI contained phenolic type amino acid groups. Fraction I of compost extract contained most of the added $^{59}Fe$ and showed weak but extra definite absorption in the 1230, and $1270cm^{-1}$ region, suggesting that extra oxygen groups in polyphenolic structure were probably involved in Fe chelation. In sewage extract, fraction I,II, and III in which most of the $^{59}Fe$ was found, showed strong definite polypeptide absorption in the region of $1540cm^{-1}$ due to NH deformation and C-N stretching of amide groups in the peptidebond. These extra functional groups in fraction I, II, and III appeared to be associated with Fe chelation. The other fractions, not associated with $^{59}Fe$, still have carboxyl and hydroxyl groups, suggesting that these functional groups in these water extracts may not independently form the Fe chelates. Precipitation of ferric hydroxide precluded measuring the stability constants for Fe-chelates. However, the formation constants for Zn chelates as log K values for compost extract and sewage extract at pH 4.0 from which the strength of chelation with Fe could be presumed, were 8.23, and 9.75, respectively, indicating strong complexation with metals. The chelating capacity of compost extract containing 6.5 g organic matter per liter was 0.82 mM, and that of sewage extract containing 5.3 g per liter was 0. 64 mM.

• Journal of fish pathology
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• v.2 no.1
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• pp.19-30
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• 1989

The purpose of this study was to obtain the fundamental data for health assessment of cultured fishes. A series of hematological studies and blood chemistries were made on cultured israeli carps from May, 1988 to May, 1989. The results of this study were as followings : 1. The blood constituents of healthy carps were Hct $32{\pm}3.4%$, Hb $8.3{\pm}0.9g/l$, RBCs $132{\pm}27.6({\times}10^4cm^3)$), Total protein $2.7{\pm}0.7g/dl$, GOT $143{\pm}19.5U$, GPT $50{\pm}14.2U$, Total glucose $70{\pm}12.6mg/dl$ and BUN $7.5{\pm}1.9mg/dl$, etc. 2. The blood constituents by change of water temperature with the control of $23^{\circ}C$ showed the decrease in Hct and RBCs at $18^{\circ}C$, and the increase in Hct, RBCs and glucose at $28^{\circ}C$. 3. The blood constituents by change of dissolved oxigen with the control of 4.5ppm showed the increase in Hct and RBCs at 3ppm, and the decrease in Hct and RBCs at 7ppm. 4. In the case of intramuscular injection of Streptococcus sp. with $10^6cells$/fish infection dose, there showed marked decrease in Hct, RBCs, glucose and BUN with inflammatory reaction, and the fishes were recovered in 16 days. 5. In the case on intraperitoneal injection of Streptococcus sp. with $10^4cells$/fish infection dose, there showed decrease in Hct, RBCs and BUN with inflammatory reaction, but there were no dead cases. 6. In the case of intramuscular injection of Edwardsilla tarda with $1.2{\times}10^8cells$/fish infection dose, there showed decrease in Hct and RBCs, and increase in TCHO, GOT, GPT and BUN with marked inflammatory reaction, and 60% of inoculated fishes were died within 3 or 4 days.

• The Korean Journal of Mycology
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• v.40 no.4
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• pp.244-253
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• 2012

The effect of fermented Rhus verniciflua stem bark (FRVSB) extract on the microbial count, enzyme activity, concentrations of free amino acids and organic acids, and physiochemical properties of doenjang (soybean paste) was evaluated during brine fermentation. The FRVSB extract increased the total free amino acid concentration by 1.3-3.1-fold on the $42^{nd}$ day of brine fermentation. After the filtration of brine, the following microbial counts were obtained in the doenjang: bacteria, $0.3{\times}10^8-12.0{\times}10^8$ cfu/g; mold, $3.0{\times}10^4-21.0{\times}10^4$ cfu/g; yeast, $1.0{\times}10^4-2.0{\times}10^4$ cfu/g; Escherichia coli, not detected; and Bacillus cereus, $3.0{\times}10^2-25.0{\times}10^2$ cfu/g. The FRVSB extract addition enhanced the protein and starch degrading activity by 13.8-26.0% and 16.1-35.1%, respectively. The extract increased the total free amino acid content by 1.4-3.0-fold. Lactic acid, acetic acid, and pyroglutamic acid were the predominant organic acids in doenjang. Moreover, the proximate composition, pH, moisture, ash, salt, and amino nitrogen content were increased.

• Journal of Preventive Medicine and Public Health
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• v.17 no.1
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• pp.95-106
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• 1984

To acquire the essential basic data to the establishment of control measure for the hazardous health effect that could be caused by harmful metals, the author measured the concentrations of trace metals in whole blood of women of $20{\sim}39$ years old living in urban and rural area using atomic absorption spectrophotometer. The summarized results were as follows; 1. The mean concentration of zinc in whole blood was $10.69{\pm}8.07{\mu}g/ml$ in rural area. The frequency distribution by zinc concentration level was nearly L-type and the cumulative frequency distribution was showed bimodal type in both area. 2. The mean iron concentration in whole blood was $323.09{\pm}87.15{\mu}g/ml$ and $322.07{\pm}104.74{\mu}g/ml$ in urban and rural area, respectively. The frequency distribution was similar to normal distribution type in both area, but the cumulative distribution was unimodal type in urban area and bimodal type in rural area. 3. The mean magnesium concentration was $41.08{\pm}19.58{\mu}g/ml$ and $40.28{\pm}16.82{\mu}g/ml$ in the area, respectively. The frequency distribution type had skewness to the right and the cumulative frequency distribution was unimodal type in both area. 4. The mean copper concentration was $1.417{\pm}0.761{\mu}g/ml$ and $1.375{\pm}0.743{\mu}g/ml$ in the area, respectively. The frequency distribution type had skewness to the right and the cumulative frequency distribution was bimodal type in both area. 5. The mean manganese concentration was $0.079{\pm}0.039{\mu}g/ml$ and $0.07{\pm}0.058{\mu}g/ml$ in the area, respectively. The frequency distribution type had skewness to the right in both area but slight irregular in rural area and the cumulative distribution was unimodal and bimodal type in urban and rural area, respectively. 6. The mean cadmium concentration in whole blood was $0.031{\pm}0.026{\mu}g/ml$ in urban and $0.028{\pm}0.023{\mu}g/ml$ in rural area. The frequency distribution type had skewness to the right and cumulative frequency distribution was bimodal type in both area.

• Korean Journal of Poultry Science
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• v.42 no.4
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• pp.335-345
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• 2015

This study was conducted to compare carcass yield and meat quality among four lines of Korean native chickens (Yeonsan Ogye, Hyunin Black, Hoengseong Yakdak and Hwangbong) and White Leghorn as control. Chickens (N=23, male) were grown under same conditions and slaughtered at 56 weeks old to observe the physicochemical differences in breast and leg meats. The live and carcass weights of Hwangbong were significantly higher than other chickens (p<0.05). Four lines of Korean native chickens, regardless of the part, had higher shear force value than White Leghorn (p<0.05). In addition, the breast meat of Korean native chickens had higher cooking loss and lower water holding capacity than that of White Leghorn. The cooked meat of Hwangbong particularly were more chewy, gummy and cohesive with lower tenderness than other chickens (p<0.05). Oleic acid content which is related to meat flavor was significantly higher in the breast meat of Hoengseong Yakdak (39.6%). The meat of Korean native chickens had lower n6/n3 ratio, in which the breast and leg meat of Hoengseong Yakdak contained the lowest ratio (p<0.05). The meat of Hwangbong and Hoengseong Yakdak were more acceptable than the others according to sensory test (p<0.05).

• Journal of Korean Society of Transportation
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• v.25 no.5
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• pp.125-133
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• 2007

Through open-sky policy, USA and European selected market principle of multiuser. However, in Asian case, major airlines monopolize airports. It is purpose that analyzes fare competition of Asian Hub Airport and the position of Incheon airport in Asia. Passengers required longer time and distance to go to the destination because direct flights decreases. But passengers increased in airport every year. Because of routes that decrease, airlines provide more services of flights. So airlines prefer to Hub Airports. As a result, both passengers and airlines are profitable by various routs and the increased frequency. On the assumption that distance and fare are related, the final formula is as following that defined the air fare from hub(H) to destination(Z) by logarithm. Analysis showed that log Rdist is not 1 but 0.08. As distance increases, fare doesn't increase. If distance from hub to destination airports is longer, Log dist_HZ is negative. It is that fare decreases from origin to destination via hub or that fare increases from hub to destination. HHI_HZ and HHI_AZ are negative. It means that if the degree of monopolization of hub and origin airports is lager, fare decreases from origin to destination via hub. Or fare increases from hub to destination. And it compares the Incheon airport with the other Asian hub airports and it examines the competitive fare by market division. As compared with the Incheon airport, Singapore, Beijing and Narita airports are higher fares. They compete with the other ones by Asian hub airports. But Hong Kong and Taipei airports must have more passengers through fare competition yet.

• Radiation Oncology Journal
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• v.15 no.4
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• pp.357-367
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• 1997

Purpose : To identify variable prognostic factors and analyse failure patterns in the uterine cervix cancer after radical operation and adjuvant radio-therapy, a retrospective analysis was undertaken. Materals and Methods : I analysed one hundred and twenty four patients with uterine cervix cancer, FIGO stage IB, IIA and IIB, treated with radical hysterectomy and pelvic lymph node dissection followed by adjuvant radio-therapy between May 1985 and May 1994. Minimum follow up period was 24 months. All of them were treated with full dose external radiotherapy with linear accelerator and/or high dese rate intracavitary radiation. Results : Overall 5 year survival rate and relapse free survival rate were $75.4\%,\;73.5\%$, respectively. Significant prognostic factors by relapse free survival were wall involvement thickness, lymph node location and number, parametrium involvement, tumor size, stage, uterine body involvement, vaginal resection margin involvement. By multivariate analysis, lymph node matastasis. tumor size and vaginal resection margin involvement were significant prognostic factos. Treatment related failure were 33 cases. Locoregional failure were more likely in the stage IIB, lymph node positive or vaginal resection margin positive patients whereas distant failures were relatively more frequent in stage IB, IIA and lymph node, vaginal resection negative patients. In stage IIB, 5 year relapse free survival rate was only $56\%$ and nine of twenty two patients recurred. Conculsion : Postoperative radiotherapy results are good for patients with relatively low risk factor. But the results are poor for patients with multiple, high risk factors or stage IIB. To control recurrence for patients with high risk factors, postoperative adjuvant radiotherapy is not sufficient treatment method. To raise control rate adding other methods such as radiosensitizing agent or chemotherapy is necessary and prospectively randomized study is needed for evaluation of postoperative radiotherapy efficacy and /or other methods. And it is reasonable to treat primary radical radiotherapy for patients with stage IIB cervical cancer instead of radical operation and adjuvant radiotherapy and/or chemotherapy regimen.

• Korean Journal of Weed Science
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• v.3 no.2
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• pp.166-173
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• 1983

To investigate the varietal differences of weed competitive functions under the three different seasons of transplanting, rice cultivars Milyang 23 and Sadominori were used with seven weeding methods, namely, weedy check, weed-free, once weeding at 3, 6, 9, 12 weeks after transplanting, and two times weeding at 3 and 9 weeks after transplanting, respectively. As a result, weeding stages and times for the proper weeding efficacies and reasonable rice productivities by respective weed group and total weed flora were estimated. Also, the constructive characteristics for yield formations under the different competitive conditions as affected by various cropping patterns were discussed.

• Korean Journal of Weed Science
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• v.3 no.2
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• pp.174-189
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• 1983

Experiments were conducted to evaluate the herbicidal characteristics of pyrazolate [4-(2,4-dichloro benzoyl)-1,3-dimethylpyrazol-5-yl-p-toluene-sulphonate] in greenhouse and lowland rice field. Pyrazolate controlled effectively most of annual weeds and such perennial weeds as Sagittaria pygmaea MIQ., Potamogeton distinctus A. BENN, Sagittaria trifolia L., Cyperus serotinus ROTTB, and Scirpus hotarui OHWI., whereas Eleocharis kuroguwai OHWI. was tolerent to pyrazolate. Although pyrazolate was applied at 2 to 10 days after transplanting, there was no difference in weed control The weeding effect was not influenced by percolation, depth of water and soil type. No difference in crop injury of rice was found with various levels of seedling age, transplanting depth, percolation, depth of water, soil type and time of application. When combined with butachlor, the mixture gave the same effect on rice phytotoxicity and weed control as pyrazolate alone did. Pyrazolate moved 1 to 2cm downward in lowland soil regardless of soil type and percolation. The herbicidal activity of pyrazolate persisted in soil for 60 to 90 days, depending on soil type, percolation and presence of soil microorganism.

• Korean Journal of Weed Science
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• v.3 no.2
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• pp.208-222
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• 1983

To control ideally the weeds in orchards and systematize the utility of herbicides, the mixing effect of oxyfluorfen and paraquat was evaluated. Combinations of oxyfluorfen with paraquat were increased the control efficacies at any treatment without giving damage to fruit trees and appeared a synergism. The control effects were reduced from 80-90% to 40-60% as the time advanced. However, the relative synergistic index was higher at 60 days after treatment in preemergence treatment and at 90 days after treatment in early and late postemergence treatment, respectively. Also the index was relatively higher at lower dosage of both herbicides.