• Korean Journal of Plant Tissue Culture
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• v.21 no.4
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• pp.239-246
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• 1994

The genotypic (2n, 3n, 4n) response of watermelon in vitro shoot tip culture was evaluated. Different genotypes had similar response in terms of shoot formation and growth. Shoot formation was better at lower concentration of 0.3 mg/L BA and higher concentration of 5-10.0 mg/L 2iP and kinetin, but growth of newly formed shoot was inhibited. With further subculture, kinetin did not promote shoot formation Better shoot formation was observed at 0.3-0.5 mg/L BA. Combination of 0.3 mg/L BA and 0.3-0.5 mg/L BA was effective in shoot multiplication, growth and induction of more internodes. Varrying levels of light intensity and agar concentration did not affect the performance of tetraploid plants. Higher light intensity and agar concentrations decreased the number of shoot formed in triploid plane. Growth in both genotype, however was inhibited. Higher light intensity was found to promote leaf senescence in all genotypes. All growth inhibitors decreased the number of shoots formed and slowed plant growth there by prolonging duration of cultures. Growth inhibitors were to observed to decrease incidence of hyperhydricity in culture. No difference in shoot formation was observed in each of the concentrations used in Ancymidol, TIBA, CCC and PP333. Shoot formation and growth was more inhibited in ABA treatments. Leaf expansion and growth was poor in all treatments.

• Proceedings of the Korea Water Resources Association Conference
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• 2016.05a
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• pp.574-574
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• 2016

유역으로 유입되는 오염물질의 발생원은 점오염원과 비점오염원으로 구분할 수 있으며 점오염원의 경우 생활하수, 산업폐수, 그리고 축산폐수에 대해 처리시설 확충 및 기술개발을 통해 관리하고 있다. 비점오염원에서의 오염물질 유출은 토지이용, 강우, 불특정적인 오염물질 투입상태 등에 따라 다르며, 지역적 특성에 영향을 받기 때문에 불확실성이 고려되어야 한다. 특히 농촌지역에서의 비점오염은 접근이 어렵고 관리주체가 모호하여 좀처럼 규명되지 않았으며, 전국적으로 그 영향이 정량화되지 않아 실질적인 관리 및 대책마련에 어려움이 있었다. 특히, 가축분뇨의 발생으로부터 처리, 자원화에 이르기까지 각 관리체계에 있어서 축산비점오염의 배출경로와 수계오염부하량, 수질환경 영향을 정밀하게 분석하여 향후 대책마련을 위한 기초자료를 확보할 필요가 있다. 또한 전국적으로 비점오염이 수계에 미치는 영향과, 그 중 축산비점오염의 영향을 면밀히 분석하여 향후 정책 및 제도개선을 위한 과학적 기초자료로서 활용할 필요가 있다. 따라서 본 연구에서는 축산 밀집 지역을 대상으로 오염원 조사를 통해 강우시 비점오염 모니터링 지점을 선정 하였으며, 년5회씩 강우 모니터링을 하여 기초데이터를 축적 하였다. 대상지역은 강원도 횡성군에 위치한 일리천 유역이며 농가 수는 총 90개의 농가가 위치하고 있는데 그 중 돼지 1,467마리, 한우 1,957마리, 젖소 581마리, 개 2,880마리, 닭 75,000마리, 사슴 4마리로 조사되었다. 대상유역을 대상으로 배출부하량을 조사한 결과 BOD 배출부하량은 총 509.3 kg/day, T-N 배출부하량은 총 331.5 kg/day, T-P 배출부하량은 총 28.3 kg/day로 조사되었다. 유출특성을 파악하기 위하여 유량가중평균농도(Event Mean Concentration, EMC)를 산정한 결과 BOD의 경우 MW-4에서 1.2 mg/L - 7.2 mg/L, MW-5에서 0.8 mg/L - 6.3 mg/L, MW-7에서 0.7 mg/L - 5.2 mg/L의 범위를 보였다. T-N의 경우 MW-4에서 1.426 mg/L - 5.321 mg/L, MW-5에서 1.205 mg/L - 4.27 mg/L, MW-7에서 0.989 mg/L - 3.859 mg/L의 범위를 보였다. T-P의 경우 MW-4에서 0.245 mg/L - 0.632 mg/L, MW-5에서 0.236 mg/L - 0.596 mg/L, MW-7에서 0.213 mg/L - 0.521 mg/L의 범위를 보였다. 본 연구에서 EMC를 산정한 방법은 평시 수질 및 유량을 정하는 기준에 따라 값이 많이 달라질 수 있다. 따라서 합리적으로 평시 부하량을 제외하고 강우시의 영향을 파악할 수 있는 EMC 산정방법에 대한 추가적인 고찰이 필요할 것으로 사료된다.

• Journal of Forest and Environmental Science
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• v.9 no.1
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• pp.44-56
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• 1993

The situation and the problems of the management of public forest owend by Kangweon provincial and Taebaek municipal govenments were investigated throughout literature and field survey. The results were as follows; 1. Kangweon provincial forest is 25,896ha and Taebaek municipal forest is 550ha, which consists of Kangweon provincial forest, 470ha and municipal forest, 80ha. Taebaek city is responsible for the management of the province-owned forest in its administrative district in addition to its own forest. 2. Except San 1-2Changjookdong (443ha) which is leased by Dai Han Coal. Coporation for mining coal, the remaining forest (107ha) consists of 32 scattered, small-area forest lands. 3. Due to small area and dispersal of municipal forest, the management base for forest production is not sound and the management at present is nearly absent. Based on these actual conditions, the following suggestions are provided for the rationalization for the municipal forest management. First, scattered, small-scale forests should be gathered to prepare a management complex. Second, the sustaining production of timber should be attained through the estblishment of systematic management plan. Third, the reasonable system for forest land utiliztion shoule be established in consideration of the recreational use of public forest.

• Science of Emotion and Sensibility
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• v.1 no.1
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• pp.153-160
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• 1998

본 연구는 피부에 가해지는 직물촉각 자극에 따라 나타나는 정서와 관련된 뇌파특징을 알아보려는 기초연구이다. 실험l에서는 촉각에 의한 정서가 생리적인 측정치를 통해 구분될 수 있는지를 알아보고자, 피험자에게 물리적 특성이 극단적으로 다른 자극을 선택하여 실험을 실시하였다. 20명의 대학생 치험자 (20-25세)에게 자극이 무선적으로 제시되었고, 각 자극제시후 피험자는 제시자극에 대한 느낌을 주관적 정서척도로 평정하였다. 뇌파는 양쪽 귓볼(A1, A2)을 기준전극으로 하여 F3, F4, C3, C4, T4, P3, P4, O1, O2에서 측정되었고, 측정된 뇌파는 FFT방법으로 분석되었다. 각 피험자의 주관적 평가 결과에 따라 가장 쾌한 자극과 가장 불쾌한 자극이 제시되었을 때 측정한 뇌파의 분석결과를 비교하였다. 전두엽, 두정엽, 후두엽 영역에서 쾌자극 제시시에 alpha파가 유의미하게 높은 값을 나타내었고, 후두엽 영역에서 불쾌자극 제시시 beta가가 유의미하게 높게 나타났다. 물리적 특성이 유사한 다섯 가지 직물자극을 가진 실험 ll에서와 유사한 절차에 다섯 가지 직물자극을 가진 실험 1에서와 유사한 절차에 의해 실험ll가 실시되었다. 실험l의 결과에 근거하여 측정 부위를 F3, F4, P3, P4, O1, O2로 축소되었다. 쾌자극 제시시의 뇌파는 불쾌자극 제시시보다 slow alpha(8-10Hz)파의 출현량이 많은 반면, slow beta(13-20Hz)파의 출현량은 적었다. 결론적으로 직물촉각자극에 의해 유발되는 심리적 반응에 특정적인 뇌파반응이 존재하며, 이는 촉각에 의해 유발되는 주관적인 쾌.불쾌정서를 객관적으로 측정하기 위한 지표로서 뇌파특징을 사용할 수 있음을 의미한다.

• Journal of Ginseng Research
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• v.24 no.2
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• pp.68-73
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• 2000

To elucidate the effect of chitin and chitosan on the production of ginsenosides and growth, ginseng hairy root was cultured on the 1/2 MS medium supplemented with chitin and chitosan of various concentrations and culture period. The highest growth was obtained with 1 mg/L of chitin. However, the growth was inhibited by 20 mg/L or above. The contents and productivity of ginsenosides were the highest when ginseng hairy roots were cultured on 40 mg/L chitin and applied of the third-weeks of culture period. Ginseng hairy root culture with 1 mg/L of chitosan resulted in the best growth, but the highest ginsenosides level was appeared in 30 mg/L chitosan. Ginsenosides content was increased when it was treated at the forth-week after culture as 30 mg/L of chitosan.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.431-438
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• 2024

Hops enhance beer flavor and bitterness, acting as a preservative. In recent years, the booming trend of craft beer has prompted the introduction of foreign hop varieties for cultivation and production in South Korea. This study focuses on developing efficient in vitro culture condition of the hop (Humulus lupulus L.) variety 'Cascade' for treatments of plant growth regulators, i.e. IAA and Cytokinin. Using Auxin IAA and Cytokinin 2iP, Zeatin, BAP, and TDZ on MS medium as plant growth regulators, the experiment involved removing three nodes from the shoot apex. In vitro hop culture showed the highest shoot proliferation rate when only IAA was added, with approximately 21% higher compared to the combination with Cytokinin. Notably, IAA 0.1 mg/L + BAP 1 mg/L resulted in a superior shoot proliferation rate of around 91%. IAA 0.1 mg/L + BAP 1 mg/L was advantageous for shoot elongation. Callus induction occurred with TDZ, while control or IAA-only conditions exhibited shoot and root growth. Cytokinin addition led to callus formation and increased weight. Assessing survival and soil adaptation during in vitro hop seedling acclimatization involved maintaining near 100% humidity initially, gradually reducing it over three weeks. When transferred outdoors, 9 out of 10 seedlings acclimated successfully, confirming a 90% acclimatization rate.

• Korean Journal of Plant Tissue Culture
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• v.26 no.4
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• pp.259-264
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• 1999

In order to study the role of ethylene on the formation of adventitious roots, trichomes and calli, the effects of 1-aminocyclopropane-l-carboxylic acid (ACC), ethephon, $CoCl_2$ and $AgNO_3$ were investigated in the leaf segments from ecotype Columbia of Arabidopsis thaliana. When the leaf segments were cultured on the media for forming adventitious roots (0.1 mg/L NAA), trichomes (2.0 mg/L NAA) and calli (10.0 mg/L NAA), and then each cultures was treated with 1-100 mg/L of ACC and ethephon, respectively. On the adventitious root-forming medium adventitious root formation was decreased, and trichomes were induced. And on the trichome-forming medium trichome formation was decreased, and calli were induced. In order hand each culture was treated with 1-100 mg/L of $CoCl_2$ and $AgNO_3$, respectively. On the adventitious root-forming medium adventitious roots was increased without trichome formation, and on the trichome-forming medium trichome formation was decreased, and adventitious roots were induced. However on the callus-forming medium treated with ACC, ethephon, $CoCl_2$ and $AgNO_3$, respectively, callus formation was inhibited and trichomes were induced in all cultures.

• Journal of radiological science and technology
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• v.29 no.4
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• pp.229-236
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• 2006

Purpose: This study was conducted to search for the type of technical error in DEXA(dual-energy X-ray absorptiometry) and the effect of error to measurement of BMD. Materials and Methods: The changes of BMD($g/cm^2$, T-score) by patients information(Age, Weight, Height, Manopause age) input error and Confirming ROI error were investigated. Using spine phantom, we canned 10 times by age(5, 10), weight(10, 20 kg), height(5, 10 cm), manopause age(5, 10) increase & decrease respectively. Scanning region(L-spine, femur, Forearm) of 10 patients was calculated by changing ROI respectively. Analysis of difference for mean(precision 1%) were carried out. Results: The error of patient information(Age, Weight, Height, Manopause age) was not changed differently. In confirming ROI, the BMD and T-score of L-spine involving T-12 was decreased to $0.063\;g/cm^2$, 0.3 and involving L-5 increased to $0.077\;g/cm^2$, 0.5. In narrowing 1 cm of vertical line of ROI, the BMD and T-score decreased to $0.006\;g/cm^2$, 0.1 and in 2 cm, $0.021\;g/cm^2$, 0.15, each. In hip ROI, Upper and left shift(0.5 cm) of line was not influenced BMD and T-score. In 0.5 cm lower shift(lesser trochanter below), the BMD and T-score increased $0.031\;g/cm^2$, 0.3 and in 1 cm $0.094\;g/cm^2$, 0.65, each. In forearm ROI, the BMD and T-score decreased $0.042\;g/cm^2$, 0.9 involving 1 cm lower wrist. And expanding 1 cm of vertical line, the BMD and T-score decreased $0.008\;g/cm^2$, 0.1 and in 2 cm, $0.021\;g/cm^2$, 0.3, each. The L-spine, hip, forearm ROI error was changed differently. Conclusion: There are so many kinds of technical error in BMD processing. Errors according to age, weight, height, manopause age did not influent to $BMD(g/cm^2)$ and T-score. There are mean differences BMD and T-score in confirming ROI. For the precision exam, in L-spine processing, L1-4 have to confirmed without shift of ROI vertical line. In hip processing, the ROI have to included greater trochanter, femur head and lesser trochanter. In forearm processing, the ROI have to included wrist, radius and ulnar.

• Journal of Food Hygiene and Safety
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• v.33 no.1
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• pp.65-70
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• 2018

Monitoring of Listeria monocytogenes, the causative agent of listeriosis, in food is inportant for public health. The Korean Food Standards Codex has adopted a 'zero-tolerance' policy for L. monocytogenes. The standard detection method of L. monocytogenes is based on enrichment. Thus, proper enrichment methods need to be instituted to ensure quality control of the detection procedures. In this study, the growth of L. monocytogenes and Listeria innocua as a mixed culture in Listeria enrichment broth (LEB) was monitored during artificial contamination of enrichment culture. We confirmed competitive growth or interspecies inhibitory activity of L. monocytogenes and L. innocua. Interspecies growth differences and the inhibitory activity of different inoculation and mixtures L. innocua against L. monocytogenes were examined. The concentration of L. monocytogenes must be 2.0 log CFU/mL or more than L. innocua to grow better than L. innocua. It is known that Listeria spp. and L. monocytogenes show growth difference during LEB, resulting in the risk of false-negative results. The inhibition of L. monocytogenes by L. innocua was always observed when present at lower concentrations. However, it was confirmed that L. innocua suppressed when L. monocytogenes was present at a higher concentration. Therefore if a mixture of Listeria spp. is present, detecting L. monocytogenes is difficult. Thus, a new enrichment broth to improve the detection rate of L. monocytogenes is needed.

• The Korean Journal of Nuclear Medicine Technology
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• v.14 no.2
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• pp.172-176
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• 2010

Purpose: The patients' moves occurred at PET/CT scan will cause the decline of correctness in results by resulting in inconsistency of Attenuation Correction (AC) and effecting on quantitative evaluation. This study has evaluated the utility of reconstruction method using AC position changing method when having inconsistency of AC depending on the position change of emission scan after transmission scan in obtaining PET/CT 3D image. Materials and Methods: We created 1 mL syringe injection space up to ${\pm}2$, 6, 10 cm toward x and y axis based on central point of polystyrene ($20{\times}20110$ cm) into GE Discovery STE16 equipment. After projection of syringe with $^{18}F$-FDG 5 kBq/mL, made an emission by changing the position and obtained the image by using AC depending on the position change. Reconstruction method is an iteration reconstruction method and is applied two times of iteration and 20 of subset, and for every emission data, decay correction depending on time pass is applied. Also, after setting ROI to the position of syringe, compared %Difference (%D) at each position to radioactivity concentrations (kBq/mL) and central point. Results: Radioactivity concentrations of central point of emission scan is 2.30 kBq/mL and is indicated as 1.95, 1.82 and 1.75 kBq/mL, relatively for +x axis, as 2.07, 1.75 and 1.65 kBq/mL for -x axis, as 2.07, 1.87 and 1.90 kBq/mL for +y axis and as 2.17, 1.85 and 1.67 kBq/mL for -y axis. Also, %D is yield as 15, 20, 23% for +x axis, as 9, 23, 28% for -x axis, as 12, 21, 20% for +y axis and as 8, 22, 29% for -y axis. When using AC position changing method, it is indicated as 2.00, 1.95 and 1.80 kBq/mL, relatively for +x axis, as 2.25, 2.15 and 1.90 kBq/mL for -x axis, as 2.07, 1.90 and 1.90 kBq/mL for +y axis, and as 2.10, 2.02, and 1.72 kBq/mL for -y axis. Also, %D is yield as 13, 15, 21% for +x axis, as 2, 6, 17% for -x axis, as 9, 17, 17% for +y axis, and as 8, 12, 25% for -y axis. Conclusion: When in inconsistency of AC, radioactivity concentrations for using AC position changing method increased average of 0.14, 0.03 kBq/mL at x, y axis and %D was improved 6.1, 4.2%. Also, it is indicated that the more far from the central point and the further position from the central point under the features that spatial resolution is lowered, the higher in lowering of radioactivity concentrations. However, since in actual clinic, attenuation degree increases more, it is considered that when in inconsistency, such tolerance will be increased. Therefore, at the lesion of the part where AC is not inconsistent, the tolerance of radioactivity concentrations will be reduced by applying AC position changing method.