• Title/Summary/Keyword: klebsiella pneumoniae

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유지산업 부산물을 이용한 1,3-propanediol 생산공정 개발

  • Kim, Seung-Hwan;Lee, Je-Hyeok;Park, Gwon-Gyu;Kim, Cheol-Ho;Lee, Sang-Gi
    • 한국생물공학회:학술대회논문집
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    • 2000.04a
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    • pp.351-354
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    • 2000
  • 1.3-Propanediol as a bifunctional organic compound could potentially be used for many synthesis reactions, in particular as a monomer for polycondensations to produce polyesters, polyethers and polyurethanes. Wastewater containing high concentration of glycerol was used to produce 1,3-propanediol in lower production cost which inturn the quantity of wastewater to be treated. In this study, various attempts were made to increase 1,3-propanediol production under different conditions by Klebsiella pneumoniae ATCC 15380. 1,3-Propanedio conversion yield and by-product formation were influenced significantly by pH and temperature. The Optimal glycerol and nitrogen concentration for 1,3-propanediol production were found to be 25g/L and 1%(w/v), respectively. The production formation of 1,3-propanediol was optimal at pH 6.0 and temperature $35^{\circ}C$.

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Gram-Negative Bacilli Isolated from Bovine Udder Infections (젖소 유방감염증(乳房感染症)으로부터 분리(分離)한 Gram 음성간균(陰性桿菌))

  • Park, Cheong Kyu
    • Korean Journal of Veterinary Research
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    • v.19 no.2
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    • pp.131-134
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    • 1979
  • In order to know the main causative agent and distribution of the agents in Gram-negative bacillus infections of bovine udders, one hundred and forty-eight cultures of Gram-negative bacilli isolated from clinical cases of mastitis during lactation over a period of 2 years in 6 herds of 180 Holstein cows were studied. Escherichia coli occupied 54.7% of the isolates was the most frequently isolated Gram-negative bacillus. 30 of the isolates (20.3%) were Enterobacter aerogenes, but Enterobacter cloacae was not isolated. Remaining isolates were Klebsiella pneumoniae, Proteus spp., Preudomonas aeruginosa and Alcaligenes faecalis. Most of the isolates (75.0%) were isolated from rear quarters.

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Dyeability and Antibacterial Activity of the Fabrics Using Balsamine Extracts (봉숭아 추출액의 염색성과 염색물의 항균성에 관한 연구)

  • 김재훈;유혜자
    • Textile Coloration and Finishing
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    • v.15 no.1
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    • pp.15-22
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    • 2003
  • Dyeing liquor was extracted from flowers, leaves and stems of balsamine. Dyeing properties, colorfastness and antibacterial activities of the fabrics dyed with the balsamine extracts were investigated at a variety of dyeing conditions of differing pH. time and temperature. Wool, silk and nylon fabrics showed better dyeability than cellulosic fabrics, i.e. cotton, linen and rayon. Especially, nylon fabrics showed the highest K/S values. The dye-uptake was achieved to the highest degree during the first ten minutes of dyeing procedure. All fabrics were dyed well at $40^\circ{C}$ with no pH adjustment. The pH of unadjusted balsamine extracts was 4.6. Their colorfastnesses to washing, drycleaning and perspiration were good as 4 or 4-5 grade. but the light fastnesses were poor as 1-2 grade. While, the antibacterial activities to Staphylococcus aureus and Klebsiella pneumoniae were excellent with 99.9% of colonies reduction ratio. Their excellent antibacterial activity was kept well after ten times washing.

Comparative In vitro Antibacterial Activity of DA-1131, A New Carbapenem Antibiotic(II)

  • Kim, Ji-Young;Kim, Gye-Won;Park, Seong-Hak;Lee, Choon-Woo;Rhee, Jae-Keol;Junnick Yang
    • Proceedings of the Korean Society of Applied Pharmacology
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    • 1996.04a
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    • pp.233-233
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    • 1996
  • 배지의 영향에 대하여 검토하기 위하여 SDA, MHA, NA 및 BHIA를 사용하였으며, 시험균주 중 Pseudomonas aeruginosa에 대하여만 NA에서의 MIC 값이 낮게 나타났을 뿐 나머지 균주에 대하여는 배지에 따른 항균력 변화가 관찰되지 않았으며, 배지의 pH를 5.4-8.4까지 변화시켰을 때 pH 변화에 따른 DA-1131의 유의적인 항균력 변화와 접종균량에 따른 항균력 변화가 관찰되지 않았다. Methicillin-resistant Staphylococcus aureus(MRSA)에 대하여는 DA-1131과 vancomycin 간의 상승 및 상가작용이 관찰되었고, P. aeruginosa, Klebsiella pneumoniae, Enterobacter cloacae 및 Serratia marcescens 등의 균종에 대하여는 ciprofloxacin의 상가작용이 확인되었다. Sisomicin은 P. aeruginosa에 대한 상승 및 상가작용이 인정되었으나 ceftazidime은 DA-1131에 의한 길항작용이 관찰되었다.

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The Isolation and Identification of Valuable Bacteria for Removal of Nitrogen and Phosphate in Municipal Waste Water (도시 하수내 질소와 인의 제거균주 분리 및 동정에 관한 연구)

  • Kim, Chul-Ho;Yoon, Sung-Nyo;Chang, Sung-Yeoul;Park, Yung-Keel
    • The Korean Journal of Ecology
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    • v.12 no.2
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    • pp.75-82
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    • 1989
  • Bacterial strains which have excellent removal capacity of phosphate or reduction capacity of were isolated from waste water. Among isolated strains. WR8 and WR1 strains were showed goood efficiency in removal of phosphate and reduction of respectively. When each strain was cultivated in waste water, WR8 strain removed about 85% of phosphate and WR1 strain reduced about 85% of -N. By the result of investigation of morphological and physiological characteristics, WR8 was identified as Aeromonas hydrophila and WR1 as Klebsiella pneumoniae.

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Antimicrobial and Water Repellency Effect of Functional Cotton Fiber with ODDMAC(octadecyl dimethyl(3-triethoxy silylpropyl) ammonium chloride) (ODDMAC를 이용한 항균성 및 발수성 동시 발현이 가능한 기능성 면섬유)

  • Jeon, Hyeji;Lee, Jaewoong
    • Textile Coloration and Finishing
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    • v.31 no.4
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    • pp.276-287
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    • 2019
  • In this study, cotton fibers were coated with a different weight percentage of octadecyl dimethyl(3-triethoxy silylpropyl) ammonium chloride(ODDMAC) to improve antimicrobial and water repellent properties. First, the ODDMAC dissolved in ethanol to prepare a solution. Then the cotton fibers were immersed in the ODDMAC/ethanol solution for 10 minutes at ambient temperature and dried at 80℃ for 3 minutes followed by curing. The treated cotton fibers were characterized by scanning electron microscopy(SEM) and x-ray photoelectron spectroscopy(XPS). The treated cotton fibers revealed sufficient antimicrobial activity against Klebsiella pneumoniae(ATCC 4352) and Staphylococcus aureus(ATCCBAA-1707). The hydrophobic nature of the treated cotton fibers was characterized by contact angle measurement. The results showed that the cotton fibers treated with the ODDMAC showed excellent hydrophobic properties which improved to 121°.

Antimicrobial Activity of Zirconium Pyrithione Complex (Zirconium Pyrithione 착물의 항균력)

  • Kwon, Chung-Moo;Rhee, Gye-Ju
    • Journal of Pharmaceutical Investigation
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    • v.18 no.3
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    • pp.107-111
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    • 1988
  • Zr, Cu, Zn and Fe-pyrithione complexes were prepared in aqueous medium and their antimicrobial activities were evaluated by MIC and cylinder plate methods against Bacillus subtilis, Sarcina lutea, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Trichophyton mentagrophytes, Candida albicans, Asperillus niger and Saccharomyces cerevisiae. Zr-pyrithione possessed most potent activities among the metal complexes against a wide range of microorganisms, especially Gram positive, B. subtilis, S. aureus and fungus, C. albicans. And all of the metal complexes synthesized were more active than pyrithione base. Fe(III)-pyrithione complex has an equal potency to Zn-pyrithione in general but it showed potent activity against B. subtilis microorganism than Zn-pyrithione.

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Clinical efficacy of ranicef$\circledR$ in the treatment of empyema thoracis (농흉에 대한 Ranicef 투여의 임상 효과)

  • Choe, Se-Yeong;Yu, Yeong-Seon
    • Journal of Chest Surgery
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    • v.23 no.5
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    • pp.894-898
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    • 1990
  • Ranicef\ulcorner[Cefpimizole sodium] is a new semisynthetic cephalosporin C derivative, was administered to 30 patients with empyema thoracis to evaluate its efficacy from Nov. 1989 to May 1990 at Department of Thoracic & Cardiovascular Surgery, Dongsan Medical Center, Keimyung University. The results were as follows. 1. The clinical efficacy of therapy was excellent in 15 cases, effective in 11 cases and failure in 4 cases. The effectiveness rate was 86.7%, respectively. 2. Among 30 cases organisms were cultured in 19 cases and 24 clinical strains were isolated. \ulcorner-hemolytic streptococcus[5], S. epidermidis[4], Pseudomonas aeruginosa[3], E. coli[3 of 5 cases], S. aureus[3 of 4 cases] and Klebsiella pneumoniae[1 of 2 cases] were effective to Ranicef\ulcorner 3. Abnormal clinical values, a decrease in platelet was observed in 3 cases and slight increases of s-GOT/GPT in 2 cases. But they were not so severe that they improved without. There were two cases of mild gastrointestinal symptom.

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Effects of Lipopolysaccharide on Pharmacokinetics of Drugs

  • Yang, Kyung-Hee;Lee, Myung-Gull
    • Toxicological Research
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    • v.23 no.4
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    • pp.289-299
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    • 2007
  • Lipopolysaccharide (LPS) endotoxin is an active component in the outer membrane of Gram-negative bacteria. LPS is usually used as an inflammatory animal model. During the inflammation, diarrhea and changes in plasma proteins, in hepatic and/or intestinal microsomal cytochrome P450 (CYP) isozymes, and in the renal and/or biliary excretion of drugs have been reported. Thus, in rats pretreated with lipopolysaccharide endotoxin isolated from Klebsiella pneumoniae (KPLPS rats), the absorption, distribution, metabolism, and excretion of drugs could be expected to be altered. Interestingly time-dependent effects on the hepatic CYP isozymes have been reported in KPLPS rats. Thus, in KPLPS rats, the pharmacokinetics of drugs which are mainly metabolized via CYP isozymes could be expected to be time-dependent. In this review, an attempt to explain changes in pharmacokinetics of drug reported in the literature was made in terms of CYP isozyme changes or urinary and/or biliary excretion changes in KPLPS rats.

Cloning and Sequenece Analysis of the hpa D Gene Responsible for Homoprotocatechuate 2, 3-Dioxgenae from Pseudomonas sp. DJ-12

  • Lee, Sang-Maha;Chae, Jong-Chan;Kim, Young-Soo;Kim, Chi-Kyung
    • Journal of Microbiology
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    • v.39 no.4
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    • pp.334-337
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    • 2001
  • The degradative pathway of homoprotocatechuate (HPC) is the bacterial routhe wherby 3,4-dihydrox-yphenylactic acid is catabolized to pyruvate and succinate by a series of sequential reactions . The HPC is catalzed by homoprotocatechuate 2, 3-dioxygenase(HPC-2,3O) to from 5-carboxymethy1-2-hydroxy-muco semialdehyde. In this study, the hha D gene encoding. HPC, 2, 3O was Cloned from the chromo-somal DNA of Pseudomonas sp. DJ-12 and its nucleotide sequence was analyzed. The open reding frame of hpaD gene was found to be composed of 864 nucleotide pairs and to encode a poypetide with 287 amino acide residues. The deduced amino acid sequence of the HPC-2,3O from Pseudomonas. sp. DJ-12 exhibited 60~64% homology with those of the corresponding enzymes from E. coli. Salmonella enterica, and Klebsiella pneumoniae.

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