• Journal of Microbiology and Biotechnology
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• 제27권2호
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• pp.297-305
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• 2017

The use of peroxidase in the nitration of phenols is gaining interest as compared with traditional chemical reactions. We investigated the kinetic characteristics of phenol nitration catalyzed by horseradish peroxidase (HRP) in an aqueous-organic biphasic system using n-butanol as the organic solvent and ${NO_2}^-$ and $H_2O_2$ as substrates. The reaction rate was mainly controlled by the reaction kinetics in the aqueous phase when appropriate agitation was used to enhance mass transfer in the biphasic system. The initial velocity of the reaction increased with increasing HRP concentration. Additionally, an increase in the substrate concentrations of phenol (0-2 mM in organic phase) or $H_2O_2$ (0-0.1 mM in aqueous phase) enhanced the nitration efficiency catalyzed by HRP. In contrast, high concentrations of organic solvent decreased the kinetic parameter $V_{max}/K_m$. No inhibition of enzyme activity was observed when the concentrations of phenol and $H_2O_2$ were at or below 10 mM and 0.1 mM, respectively. On the basis of the peroxidase catalytic mechanism, a double-substrate ping-pong kinetic model was established. The kinetic parameters were ${K_m}^{H_2O_2}=1.09mM$, ${K_m}^{PhOH}=9.45mM$, and $V_{max}=0.196mM/min$. The proposed model was well fit to the data obtained from additional independent experiments under the suggested optimal synthesis conditions. The kinetic model developed in this paper lays a foundation for further comprehensive study of enzymatic nitration kinetics.

• 한국공작기계학회:학술대회논문집
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• 한국공작기계학회 2001년도 춘계학술대회 논문집(한국공작기계학회)
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• pp.15-21
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• 2001

An accumulator in hydraulic systems stores kinetic energy during braking action, and then that controls hasty surge pressure. An energy recovery system using accumulator seems to be advantageous for ERBS due to its high energy density. This study suggests a method to decide suitable accumulator volume for ERBS. The method is based upon energy conservation between kinetic energy of moving inertia and elastic energy of accumulator. The energy conversion was analyzed and a simple formula was derived. A series of computer simulation was done to verify effectiveness of the formula. The results of the simulation work were compared with those of experiments and these results show that the proposed design is effective for decision accumulator volume in ERBS.

• 한국공작기계학회논문집
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• 제11권1호
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• pp.104-113
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• 2002

An accumulator in hydraulic systems stores kinetic energy during braking action and then that controls hasty surge pressure. An energy recovery system using accumulator seems to be advantageous far ERBS due to its high energy density. This study suggests a method to decide suitable accumulator volume far ERBS. The method is based upon energy conservation between kinetic energy of moving inertia and elastic energy of accumulator. The energy conversion was analyzed and a simple formula was derived. A series of computer simulation was done to verify effectiveness of the formu1a. The results of the simulation work were compared with those of experiments and these results show that the proposed design is effective far decision of accumulator volume in ERBS.

• 동력기계공학회지
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• 제13권3호
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• pp.27-32
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• 2009

Numerical analysis information of a complex discharge-passage will be very useful to improve hydrogen compression system. General information about an internal gas flow is presented by numerical analysis approach. Relating with hydrogen compressing system, which have an important role in hydrogen energy utilization, this should be a useful tool to observe the flow quickly and clearly. Flow characteristic analysis, including pressure and turbulence kinetic energy distribution of hydrogen gas from cylinder going to the chamber of a reciprocating compressor are presented in this paper. Discharge-passage model is designed based on real model of hydrogen compressor. Pressure boundary conditions are applied considering the real condition of operating system. The result shows pressure and turbulence kinetic energy are not distributed uniformly along the passage of the hydrogen compressing system. Path line or particles tracks help to demonstrate flow characteristics inside the passage. The existence of vortices and flow direction can be precisely predicted. Based on this result, the design improvement should be done. Consequently, development of the better hydrogen compressing system will be achieved.

• Journal of Advanced Marine Engineering and Technology
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• 제32권8호
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• pp.1201-1207
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• 2008

Numerical analysis information will be very useful to improve fluid system. General information about an internal gas flow is presented by numerical analysis approach. Relating with hydrogen compressing system, which have an important role in hydrogen energy utilization, this should be a useful tool to observe the flow quickly and clearly. Flow characteristic analysis, including pressure and turbulence kinetic energy distribution of hydrogen gas coming to the cylinder of a reciprocating compressor are presented in this paper. Suction-passage model is designed based on real model of hydrogen compressor. Pressure boundary conditions are applied considering the real condition of operating system. The result shows pressure and turbulence kinetic energy are not distributed uniformly along the passage of the Hydrogen system. Path line or particles tracks help to demonstrate flow characteristics inside the passage. The existence of vortices and flow direction can be precisely predicted. Based on this result, the design improvement, such as reducing the varying flow parameters and flow reorientation should be done. Consequently, development of the better hydrogen compressing system will be achieved.

• Membrane and Water Treatment
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• 제8권1호
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• pp.73-88
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• 2017

Adsorption kinetics of aqueous ferric ion ($Fe^{3+}$) onto bio-natural rice grains (BRG) have been studied in a batch system. The influence of contact time (0-180 minutes), the dosage of BRG adsorbent (10, 20, 40, and $60gL^{-1}$), and ambient temperature (27, 37, 47, and $57^{\circ}C$) for the adsorption system have been reported. The equilibrium time achieved after 20 minutes of adsorption contact time. The maximum removal of ferric ion is 99% by using $60gL^{-1}$ of BRG, $T=37^{\circ}C$, and $50mgL^{-1}$ ferric ion solution. Adsorption kinetic and diffusion models, such as pseudo-first order, pseudo-second order, and Weber-Morris intra-particle diffusion model, have been used to describe the adsorption rate and mechanism of the ferric ion onto BRG surface. The sorption data results are fitted by Lagergren pseudo-second order model ($R^2=1.0$). The kinetic parameters, rate constant, and sorption capacities have been calculated. The new information in this study suggests that BRG could adsorb ferric ion from water physiosorption during the first 5 minutes. Afterward, the electrostatic interaction between ferric ion and BGR-surface could take place as a very weak chemisorptions process. Thus, there is no significant change could be noticed in the FTIR spectra after adsorption. I recommend producing BGR as a bio-natural filtering material for removing the ferric ion from water.

• Journal of Korean Society for Atmospheric Environment
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• 제18권E3호
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• pp.165-174
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• 2002

A dual -column biofilter system with two different composts was used to investigate the macro-kinetics of dim-ethyl disulfide (DMDS) degradation. The biofilter columns were filled with compost mixtures up to one meter, The gas How rate and DMDS concentration to the biofilters were varied to study their effect on the removal characteris-tics of DMDS. It was found that the biodegradation of DMDS was governed by zero-order reaction -limited macro-kinetics for inlet DMDS concentrations between 10 and 55 ppmv. The overall average zero-order kinetic coeffi-cient for DMDS removal by compost was 0.50 ($\pm$0.1) ppm/sec for both compost mixtures studied. Variations in individual kinetic coefficients were observed due to varying environmental conditions, such as pH and temperature. The kinetic coefficients determined are specific to the system discussed in this work. During high acidity conditions in the filter beds, methyl mercaptan (MM) was observed in the gas samples collected. Appearance of MM was pro-bably due to decreased microbial activity in the lower portions of the biofilter. Considering the neutral pH range required and the presence of methyl mercaptan, it is likely that the microorganisms present in the biofilters used in this research are similar to the T. thioparus (strain E6) species.

• 한국군사과학기술학회지
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• 제16권6호
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• pp.828-832
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• 2013

In this paper, numerical analysis was performed about whether the flash fire of loaded shells breaks out in the virtual combat vehicle according to sorts of the kinetic energy ammunition as the preceding research for vulnerability analysis inside the combat system by an external threaty ammunition. In this simulation, Autodyn program was used and the Lee-Tarver ignition and growth model was used to determine the flash fire outbreak. In this study, the kinetic energy ammunition was set of type A and type B in two kinds and the loaded shells was set of COMPB, TNT, PBX9404 and ANB. As a result, TNT and PBX9404 have much higher flash fire probability than COMPB in high explosive, ANB has very low flash fire probability.

• 한국멀티미디어학회논문지
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• 제24권8호
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• pp.1184-1191
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• 2021

In a pop song, the creator's intention is communicated to the user through music and lyrics. Lyric meaning is as important as music, but in most cases lyrics are delivered to users in a static form without non-verbal cues. Providing lyrics in a static text format is inefficient in conveying the emotions of a music. Recently, lyrics video with kinetic typography are increasingly provided, but producing them requires expertise and a lot of time. Therefore, in this system, the emotions of the lyrics are found through the analysis of the text of the lyrics, and the deep learning model is trained with the data obtained by converting the melody into a Mel-spectrogram format to find the appropriate emotions for the music. It sets properties such as motion, font, and color using the emotions found in the music, and automatically creates a kinetic typography video. In this study, we tried to enhance the effect of conveying the meaning of music through this system.

• East Asian mathematical journal
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• 제37권1호
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• pp.141-147
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• 2021

In [1], we studied the PDE system with time-varing delay. Time delay occurs due to loosening in a high-speed moving axially directed membrane (string, belt, or plate) at production. Our purpose in this work derives a mathematical model with internal time delay. First, we consider the physical phenomenon of axially moving membrane with respect to kinetic energy, potential energy and work done. By the energy conservation law in physics, we get the second order nonlinear PDE system with internal time delay.