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Comparative study on growth of leafy vegetables grown in a hybrid BFT-aquaponics using Japanese eel, Anguilla japonica and hydroponics

  • Lee, Dong-Hoon;Kim, Jeong-Dae
    • Fisheries and Aquatic Sciences
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    • v.24 no.7
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    • pp.260-275
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    • 2021
  • Aquaponics is a cultivation system that combines aquaculture and agricultural hydroponics. This study investigated the productivity of leafy vegetables cultivated in the hybrid biofloc technology-aquaponics (HBFT-AP) using Japanese eel fed two kinds of diets (Mash, commercial powdered feed and extruded pellet [EP]) and hydroponics (HP).The mash was fed to the fish in a type of a dough mixed with water for 6 weeks (Exp1) and switched to the EP containing 2.7% monobasic potassium phosphate (MKP) for subsequent 6 weeks (Exp2). Leafy vegetables of 8 cultivar were employed in the experiment and water quality [dissolved oxygen (DO, mg/L), pH, water temperature (℃), electrical conductivity (EC; ㎲/cm), turbidity (NTU), TAN (NH3 + NH4+) (mg /L), NO2-N (mg/L), NO3-N (mg/L) and PO4-P (mg/L)] was measured 6 times a week. Leafy vegetable productivity (HBFT-AP vs HP) was compared in respective experiment, which was similar or somewhat higher in HBFT-AP. During the 12-week feeding trial, concentrations of nitrite (NO2-N) and phosphorus (PO4-P) were kept low in variability while total ammonia nitrogen (TAN) and NO3-N levels increased with time in HBFT-AP. At the end of two feeding trials, values of weight gain (WG, %), feed efficiency (FE, %), specific growth rate (SGR, %) and protein efficiency ratio (PER) were higher in Exp2 than in Exp1. As well, higher values in hematocrit (PCV, %), plasma K (mEq/L) and inorganic phosphorus (mg/dL) were found (p < 0.05) in Exp2 where fish were fed the EP (EP: 38.60%, 2.80 mEq/L and 7.04 g/dL; Mash: 33.20%, 1.95 mEq/L and 5.50 g/dL). Leafy vegetables in HBFT-AP using Japanese eel fed the EP with MKP 2.7% had a productivity similar (4 kinds of cultivar) or somewhat higher (4 kinds of cultivar) compared to those in HP. Also, Japanese eel fed the EP showed higher values of Pi (m/dL) and K (mEq/L) in plasma compared to those fed commercial powder diet.

Physicochemical Changes of Swine Manure by the Treatment of Acid and Alkali for Inactivation of Pathogenic Microorganisms (병원성미생물의 불활성화를 위한 산·알칼리처리가 양돈분뇨의 이화학적 성상에 미치는 영향)

  • Kim, Cho-Long;Kim, Soo-Ryang;Kim, Ha-Je;Jeon, Sang-Joon;Han, Ho;Kim, Dong-Kyun;Lee, Myung-Gyu
    • Journal of Animal Environmental Science
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    • v.18 no.3
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    • pp.229-234
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    • 2012
  • Disinfecting contaminated swine manure with FMD (Foot-and-Mouth Disease) and pathogenic viruses is very important for maintaining sanitized environment. However, very few research reported on this subject, especially post-disinfection to utilize the wastes as a renewable resource. This research is carried out to obtain basic information for chemical treatment in FMD SOP (Standard Operating Procedure, Korea) of contaminated swine manure. Using lime, sodium hydroxide, citric acid and hydrochloric acid, described in FMD SOP, the effects of chemical treatments on livestock manure were compared in this paper. Four combinations of alkali-acid treatments and four kinds of acid-alkali combinations were tested to find out the effective method. Total coliform bacteria in contaminated swine manure, $1.6{\times}10^4$ CFU/100 ml, decreased to the range of 1/1000~1/100 in all treatments. Some specific disinfectants increases BOD (Biochemical Oxygen Demand) and EC(Electric Conductivity), especially, alkaline treatments increases ammonia level than acid treatments. These findings suggest that the treatment methods should be considered as an important environmental factor in post-disinfection of contaminated animal manure with pathogenic microorganisms.

Comparison of an Automated Most-Probable-Number Technique TEMPO®TVC with Traditional Plating Methods PetrifilmTM for Estimating Populations of Total Aerobic Bacteria with Livestock Products (축산물가공품에서 건조필름법과 TEMPO®TVC 검사법의 총세균수 비교분석)

  • Kim, Young-Jo;Wee, Sung-Hwan;Yoon, Ha-Chung;Heo, Eun-Jeong;Park, Hyun-Jeong;Kim, Ji-Ho;Moon, Jin-San
    • Journal of Food Hygiene and Safety
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    • v.27 no.1
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    • pp.103-107
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    • 2012
  • We compared between an automated most-probable-number technique $TEMPO^{(R)}$TVC and traditional plating methods $Petrifilm^{TM}$ for estimating populations of total aerobic bacteria in various livestock products. 257 samples randomly selected in local retail stores and 87 samples inoculated with $E.$ $coli$ ATCC 25922, $Staphylococcus$ $aureus$ ATCC 12868 were tested in this study. The degree of agreement was estimated according to the CCFRA (Campden and Chorleywood Food Research Association Group) Guideline 29 and the agreement indicates the difference of two kinds methods is lower than 1 log base 10($log_{10}$). The samples of hams, jerky products, ground meat products, milks, ice creams, infant formulas, and egg heat formed products were showed above 95% in the agreement of methods. In contrast, proportion of agreement on meat extract products, cheeses and sausages were 93.1%, 92.1%, 89.1%, respectively. One press ham and five sausages containing spice and seasoning, two pork cutlets containing spice and bread crumbs, two meat extract product and two natural cheeses and one processing cheese with a high fat content, and one ice cream containing chocolate of all samples showed the discrepancy. Our result suggest that $TEMPO^{(R)}$TVC system is efficient to analyses total aerobic bacteria to compare manual method in time-consuming and laborious process except livestock products having limit of detection.

Evaluation of Nutritional Quality of Packaged MealsProduced by Packaged Meal Manufactures in Seoul and Kyungki-do (시판 도시락의 영양평가 및 품질관리 방안에 관한 연구)

  • 계승희
    • Journal of Nutrition and Health
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    • v.22 no.3
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    • pp.149-158
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    • 1989
  • To investigate the nutritional quality of packaged meals produced by packaged meal manufactures, a nutritional evaluation was conduced. Samples examined in this study were 95 Products which composed of 86 Doraks, 7 Kimplabs and 2 Yubuchopabs Produced in packaged meal manufactures in Seoul City and Kungki-do province The distribution of food groups showed that amounts of animal foods were abound, but amounts of green vegetables, limited in Dosirak comparing to one meal RDA levels. Amounts of meats, fishes and shells are increased more than amounts of egged and legumina with increase of price of Dosirak. The total kinds of food and the amounts of certain food in Kimpab and Yubuchopab were lower than Dosirak. The energy and protein contents of Dosirak were found to be higher than one meal RDA levels, with exception of protein content in Dosirak of the price 1000 won. The contents of energy and protein in Kimpab and Yubuchopab are less than one meal RDA levels. Calcium and iron contents in Dosirak, and calcium content in Yubuchopab were present in excess compared to the one meal RDA. However, In Kimpab, calcium and iron contents were lower than the one meal RDA. On the whole, vitamin contents of Dosirak were sufficient whereas vitamin contents of Kimpab and Yubuchopab were lower than the one meal RDA. Number of side dishes used in Dosirak were 6-23 items depending on prices. Guidelines were suggested for effective nutritional quality control of packaged meals.

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A Survey and Analysis of the Hygienic Aspects of Pet-Dog Clothes Materials

  • Shim, Boo-Ja
    • Journal of Fashion Business
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    • v.8 no.3
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    • pp.10-19
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    • 2004
  • The purpose of this research is to reveal the antimicrobial activity of pet-dog clothes by investigating the bacteria resistance of 4 kinds of pet-dog clothes materials. 1. Investigation Results of Pet-Dog Clothes 64.7% of 150 survey participants, revealed they had pet-dog clothes. Hand laundering was 67.0%, while the laundering of both human and animal clothes was 9.2%. The greatest washing frequency was once every 2 weeks. So, the subjects didn't seem to think high of hygienic matters. Even though no if any relations were reported by 34.7% of the subjects, there were some experiences like sneezing or coughing (41.3%), slight itching (20.7%), and acute skin allergies. There were such hugging methods as heart to heart to the center (22.7%), face to face and around the mouth (16.7%), and below the heart with the dog's face outward (15.3%). Thus, hugging the dog near the pet-lover's face seems to be the cause of respiratory diseases including sneezes and coughs. 2. Results of the Anti-Bacteria Experiment of Pet-Dog Clothes Materials According to the analysis of the germs collected and cultured in this study to reveal the properness of pet-dog clothes materials, they were bacteria or bacilli in shapes. Spore growth was active in the order of such experimental materials as artificial leather > cotton > cotton/ nylon > polyester (finest thread). In terms of germ groups, the order was polyester(finest thread) > cotton > cotton/ nylon > artificial leather.

Observation of Mechanical Strength of Materials for Dog Dental Prosthesis Production (중형견(犬) 치과 보철물 제작을 위한 소재의 기계적 강도 관찰)

  • Park, Yujin;Choi, Sungmin
    • Journal of Technologic Dentistry
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    • v.41 no.2
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    • pp.93-100
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    • 2019
  • Purpose: This study is making a dog dental prosthesis using digital dental technology. The mechanical strength of the prosthetic material was observed in terms of compressive strength and fracture pattern. Methods: The experiment was performed using dog mandibular molars. The teeth were scanned and modeled. The specimens were made of zirconia, PMMA and Ni-Cr. The specimens were subjected to a vertical compression test with an artificial cancellous bone in UTM tester. Vertical compressive strength and fracture behavior of specimen were observed. Results: The result of observing the compressive load between specimen and artificial bone were $184.8{\pm}5.7N$ in the zirconia specimen, $185.6{\pm}8.9N$ in the PMMA specimen, and $184.4{\pm}4.0N$ in the Ni-Cr alloy specimen. Compression marks of artificial bones were observed. The fracture strength of specimen was observed. The fracture strength of the zirconia specimen was an average of 1,381.4N. The fracture strength of the PMMA specimen was an average of 572.2N. Conclusion: The crown made of three kinds(zirconia, PMMA, Ni-Cr alloy) of materials has the strength to chew about the artificial bone. zirconia and PMMA have vertical compressive strength applicable to medium dog dental prosthetic materials.

Morphology of Retinas and Lenses in the Fish of the Genus Zacco (Cypriniformes, Cyprinidae): Possible Relationship with Prey and Habitat

  • Lim, Jae-Won;Lee, Chung-Lyul;Lee, Moo-Sam
    • Animal cells and systems
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    • v.7 no.4
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    • pp.331-335
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    • 2003
  • Vertebrates with different habitats have different proportions of visual cells, with the rod cells responding to scotopic vision and the cone cells responding to photopic and color vision in their retinas. The present work studied whether the kinds and arrangement patterns of the cone cells and interlocking morphology of the lens were related to the kind of preys and habitats in the genus Zacco. The retinas were observed by a light microscopy using H-E staining method and the interlocking formula of the lens fibers were investigated by a scanning electron microscopy. The interlocking formula of the lens fibers of Z. temmincki is an ' anchor and socket ' connection, and that of Z. platypus is a ' ball and socket ' connection. The cone cells of Z. platypus and Z. temmincki constituted compacted mosaic patterns of row type. Away from the center, the double and single cone cells gradually increased in diameter. Zacco temmincki had identical double cone cells and Z. platypus had non-identical double and single cone cells. The eyes of Z. temminckifeeding on a moving aquatic insects in relative limpid water and swift current of mid and upper stream have better resolution than that of Z. platypus feeding on mainly adhesive algae and some aquatic insects in slightly turbid water of mid stream.

Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis

  • Lee, Ji Hye;Kim, Keun Jung;Choi, Seon A;Li, Xiaoxia;Kim, Eun Young;Oh, Hyun Ju;Lee, Byeong Chun;Kim, Hye Jin;Park, Byung Kwon;Kim, Min Kyu
    • Korean Journal of Veterinary Research
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    • v.49 no.4
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    • pp.285-290
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    • 2009
  • This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with $1.3{\times}$ $10^8$ spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.

Animal Models for Aging and Neurodegenerative Diseases: Brain Cell Apoptosis in the Dog and its Possible Mechanisms

  • Nakayama, Hiroyuki;Kajikawa, Satoru;Doi, Kunio
    • Toxicological Research
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    • v.17
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    • pp.71-77
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    • 2001
  • The brain of the aged dog possesses senile plaques and amyloid angiopathy, which characterize Alzheimer's disease brains. We have defined the dementia condition of aged dogs and examined which mechanism(s) is responsible for the condition. A series of studies revealed that the dementia condition in aged dogs is significantly related to the number of apoptotic brain cells including both neurons and glial cells, but not to the number of senile plaques. On the other hand, 5-azacytidine (5AzC) is a cytidine analogue, and is thought to induce kinds of cell differentiation possibly through hypomethylation of genomic DNA. We have revealed neuronal apoptosis induced in 5AzC-treated fetal mice and PC12 cells. The ribosomal protein L4 (rpL4) gene is expressed prior to the apoptosis in the PC12 cell system. Therefore, the involvement of the rpL4 gene expression in age-related brain cell apoptosis in dogs may contribute to the investigation of Alzheimer's dementia.

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Zoom-in X-ray Micro Tomography System

  • Chun, In-Kon;Lee, Sang-Chul;Park, Jeong-Jin;Cho, Min-Hyoung;Lee, Soo-Yeol
    • Journal of Biomedical Engineering Research
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    • v.26 no.5
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    • pp.295-300
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    • 2005
  • We introduce an x-ray micro tomography system capable of high resolution imaging of a local region inside a small animal. By combining two kinds of projection data, one from a full field-of-view (FOV) scan of the whole body and the other from a limited FOV scan of the region of interest, we have obtained zoomed-in images of the region of interest without any contrast a nomalies. We have integrated a micro tomography system using a micro-focus x-ray source, a $1248\times1248$ flat-panel x-ray detector, and a precision scan mechanism. Using the cross-sectional images taken with the zoom-in micro tomography system, we measured trabecular thicknesses of femur bones in postmortem rats. To compensate the limited spatial resolution in the zoom-in micro tomography images, we used the fuzzy distance transform for the calculation of the trabecular thickness. To validate the trabecular thickness measurement with the zoom-in micro tomography images, we compared the measurement results with the ones obtained from the conventional micro tomography images of the extracted bone samples.