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Superfecundation induction by intrauterine insemination with different frozen-thawed canine semen and parentage test using microsatellite analysis  

Lee, Ji Hye (College of Agriculture and Life Sciences, Chungnam National University)
Kim, Keun Jung (College of Agriculture and Life Sciences, Chungnam National University)
Choi, Seon A (College of Agriculture and Life Sciences, Chungnam National University)
Li, Xiaoxia (College of Agriculture and Life Sciences, Chungnam National University)
Kim, Eun Young (College of Agriculture and Life Sciences, Chungnam National University)
Oh, Hyun Ju (College of Veterinary Medicine, Seoul National University)
Lee, Byeong Chun (College of Veterinary Medicine, Seoul National University)
Kim, Hye Jin (Haemaru Small Animal Referral Hospital)
Park, Byung Kwon (College of Industrial Science, Kongju National University)
Kim, Min Kyu (College of Agriculture and Life Sciences, Chungnam National University)
Publication Information
Korean Journal of Veterinary Research / v.49, no.4, 2009 , pp. 285-290 More about this Journal
Abstract
This study was performed to investigate the possibility of superfecundation by surgical intrauterine artificial insemination in dogs of confirmed genetic pedigree. Artificial insemination was performed on 3 days after ovulation with $1.3{\times}$ $10^8$ spermatozoa. Five puppies were delivered on 60 days after insemination. The ratio of the number of newborns to the number of corpora lutea was 83.3% (5/6). Parentage analysis with 10 canine-specific microstatellite markers demonstrated that one puppy was genetically relative to the sire-A family and four puppies were genetically relative to the sire-B. The present study demonstrated that two kinds of puppies with different genetic pedigree can be produced by surgical uterine insemination of semen of individual dog into each uterine horn of a bitch.
Keywords
frozen-thawed semen; intrauterine insemination; microsatellite; superfecundation;
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