• Title/Summary/Keyword: kasugamycin

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Studies on the Optimization of Media Composition and Cultural Conditions for Kasugamycin Production, by Streptomyces kasugaensis (Streptomyces kasugaensis의 Kasugamycin 생산배지조성 및 배양조건의 검토)

  • 오영준
    • Microbiology and Biotechnology Letters
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    • v.20 no.5
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    • pp.583-587
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    • 1992
  • This study was conducted in order to optimize the media composition and cultural conditions for kasugamycin production by Streptomyces kasugaensis. The optimum culture conditions are pH 6.6 (before sterilization) and $28^{\circ}C$ for the production of kasugamycin. The kasugamycin concentration was not increased when silicone oil as antifoam agent was added. The addition of water during the cultivation in the various media showed a positive effect for the production of kasugamycin.

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Mutagenesis of Streptomyces kasugaensis for Kasugamycin Production

  • Cho, Hoon;Choi, Du-Bok;Lim, Chae-Kyu
    • Journal of environmental and Sanitary engineering
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    • v.23 no.4
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    • pp.23-29
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    • 2008
  • This study was performed to develop mutant strain using a combination of UV irradiation procedures with protoplast mutagenesis in order to achieve an effective kasugamycin production from Streptomyceskasugaensis. Whenlessthan 1.0g/lof Linoleic acid was used, the cell growth was not inhibited. On the other hand, the cell growth was greatly inhibited when more than 1.6 g/l of linoleic acid was used. Among the various mutant strains, SK-12 was obtained in medium containing 1.6g/l of linoleic acid, showing the highest rate of both cell growth and kasugamycin production. In order to compare kasugamycin production with the SK-12 and the parent strain using soybean oil, cultures were performed in a flask. The production of kasugamycin was increased with the increase time. The maximum kasugamycin concentration was 1.2g/l after 6 days of culture. The product yield from soybean oil was 0.05g/l/g consumed carbon source, which was roughly 5.0 fold higher than the parent strain. These results show that it was effective method for obtaining a mutant resistant to linoleic acid for the effective production of kasugamycin from soybean oil.

Effect of Some Parameters for Selecting the High Kasugamycin Producing Mutants (고수율 Kasugamycin 생산 변이주의 선발시 여러 인자들의 효과)

  • 김윤정;이상한;손광희;복성해
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.131-135
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    • 1989
  • Effects of the inoculum size of testing organism and pH of the plate and the concentration of agar were investigated for the selection of high kasugamycin producing mutants of Streptomyces kasugaensis ATCC 15114. For the detection of high kasugamycin-producing mutants, both concentrations of agar and test organism were optimized at the concentrations of 2% and 0.35 (A$_{550}$), respectively. The pH 7 was optimum for both growing the testing organism, Pseudomonas fluorescens IFO 12180, and for obtaining more promising mutant strains of S. kasugaensis. Under these conditions, mutants had been isolated which, tested later in liquid cultures, gave higher kasugamycin yields than that of the parent strain.

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Development of Continuous Clean Bioprocess for Kasugamycin Production (Kasugamycin 생산을 위한 연속 청정생물공정 개발)

  • Kim, Chang Joon;Park, Sun Ok;Chang, Yong Keun;Chun, Gie-Taek;Lee, Jong-Dae;Kim, Sangyong
    • Clean Technology
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    • v.4 no.1
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    • pp.45-59
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    • 1998
  • Continuous immobilized-cell culture was carried out for the production of kasugamycin, a secondary metabolite by a filamentous bacteria, Streptomyces kasugaensis, with an intention of reducing waste generation. A sporulation medium was developed for production of bulk amounts of spores, and the spores were entrapped into celite biosupports for immobilization. It was possible to effectively keep the immobilized-cells inside the reactor during the continuous culture by an efficient immobilized cell separator of decantor type on the outlet of the fermentor. Using this continuous immobilized-cell fermentor system, we investigated the effects of feed substrate and phosphate concentrations on kasugamycin production and chemical oxygen demand(COD). Comparing with the conventional suspended-cell batch culture, the kasugamycin productivity was observed to increase by 2.5 times, whereas COD per unit kasugamycin production decreased by 2.3 times in the continuous immobilized-cell culture. Based on these results, the continuous immobilized-cell system was considered to be a cleaner bioprocess than the conventional batch suspended-cell system.

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Isolation and Characterization of Kasugamycin Biosynthetic Genes from Streptomyces kasugaensis KACC 20262

  • JO YOU-YOUNG;LIU JING;JIN YING-YU;YANG YOUNG-YELL;SUH JOO-WON
    • Journal of Microbiology and Biotechnology
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    • v.15 no.3
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    • pp.491-496
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    • 2005
  • The biosynthetic gene cluster for the aminoglycoside antibiotic kasugamycin was isolated and characterized from the kasugamycin producing strain, Streptomyces kasugaensis KACC 20262. By screening a fosmid library using kasA, the gene encoding aminotransferase, we isolated a 22 kb DNA fragment. The fragment contained seventeen complete open reading frames (ORFs); one of these ORFs, kasD, was identified as the gene for dNDP-glucose 4,6-dehydratase, which catalyzes the conversion of dNDP-glucose to 4-keto-6-deoxy-dNDP-glucose. The enzyme showed a broad spectrum of substrate specificity. In addition, ksR was overexpressed in E. coli BL21 and proved to be a self-resistance gene against kasugamycin. These findings suggest that the isolated gene cluster is highly likely responsible for the biosynthesis of kasugamycin.

Analysis of Kasugamycin in Pesticide Formulations by Reversed-Phase Ion Pair Liquid Chromatography (역상 이온쌍 액체크로마토그래피에 의한 농약 제품 중 Kasugamycin의 분석)

  • Kim, Taek-Jae;Kim, Kyong-Sun;Yoon, Chae-Hyuk;Joo, Jin-Bok;Kim, Chung-Hyo
    • Analytical Science and Technology
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    • v.10 no.5
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    • pp.343-349
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    • 1997
  • Analytical method using the reversed phase ion-pair chromatography (RP-IPC) for the determination of kasugamycin(5-amino-2-methyl-6-(2,3,4,5,6-pentahydroxy cyclohexyloxy)tetrahydropyran-3-yl-amino-${\alpha}$-imino acetic acid), pesticide as fungicide bactercide has been established. The retention behavior of kasugamycin in the RP-IPC was examined with respect to the effect of concentrations of organic modifiers, pH of eluent and types and concentrations of the counter ions as ion-pair reagent. This method developed by the optimum factors, can be used for the application of the quality control in the crude product and its formulation.

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Effect of some antibiotics on control of bacterial brown blotch of oyster mushroom caused by Pseudomonas tolaasii (몇가지 항생제의 느타리버섯 Pseudomonas tolaasii에 의한 세균성갈색무늬병 방제 효과)

  • Seo, Geon-Sik;Jeon, Nak-Beom;Kim, Byung-Ryun;Yu, Seung-Hun
    • Journal of Mushroom
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    • v.1 no.1
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    • pp.15-20
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    • 2003
  • Antibacterial activities of 33 antibiotics against Pseudomonas tolaasii causing the brown blotch disease on the edible mushroom Pleurotus ostreatus, were tested in vitro for the control of the disease. Tetracyclin, kanamycin, kasugamycin, and streptomycin showed strong antibacterial activity against P. tolaasii, having the minimal inhibitory concentration of 10, 10, 100 and 200ppm, respectively. These antibiotics showed similar control value of 72.9, 71.2, 68.1 and 62.7%, respectively when applied on the artificially infected mushroom beds. Mushroom yields in the tetracycline treated boxes were increased about 31.8% comparing to the control ones. Mycelial growth of P. ostreatus on the PDA supplemented with streptomycin and kanamycin were not affected, but were inhibited 10~20% and 40% with tetracyclin and kasugamycin treatment, respectively.

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Physiological Characteristics of Immobilized Streptomyces Cells in Continuous Cultures at Different Dilution Rates

  • Kim, Chang-Joon;Chang, Yong-Keun;Chun, Gie-Taek;Jeong, Yeon-Ho;Lee, Sang-Jong
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.557-562
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    • 2002
  • Physiological characteristics such as specific productivity, morphology of Streptomyces cells Immobilized on celite beads, and operational stability at different dilution rates were investigated in continuous immobilized-cell cultures for the production of kasugamycin. At a dilution rate (D) of 0.05 $h^{-1}$, a relatively high specific productivity was attained and the loss of cell-loaded beads was negligible. At D=0.1 $h^{-1}$, a higher specific productivity and cell concentration could be obtained, resulting in a significantly improved volumetric kasugamycin productivity. However, no stable operation could be maintained due to a significant loss of cell-loaded beads from the reactor that was caused by their fluffy morphology developed in the later stage. At D=0.2 $h^{-1}$, the production of kasugamycin and cell growth were observed to be severely inhibited by the high concentration of residual maltose.

Chemical Control of bacterial Canker of Kiwifruit (참다래 궤양병의 약제 방제)

  • 고영진
    • Plant Disease and Agriculture
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    • v.5 no.2
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    • pp.95-99
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    • 1999
  • Chemical control of bacterial canker of kiwifruit caused by Pseudomonas syringae pv. actinidiae was attempted by spraying of streptomycin sulfate ·oxytetracycline WP streptomycin WP streptomycin ·copper hydroxide WP kasugamycin SL kasugamycin·copper oxychloride WP and copper hydroxide WP. The control efficacies of the bactericides were variable depending upon the spraying schedule,. Application of streptomycin WP and streptomycin sulfate·oxytetracycline WP from middle April to early May was found to be the most effective in controlling the bacterial canker. For copper hydroxide WP the spraying from middle January to early February showed the highest control efficacy. Kasugamycin SL was the most effective in controlling the disease by spraying from middle April to early May but it was still relatibvely effective during other spray periods. Foliar application of copper hydroxide WP and copper-antibiotic formulaions after middle April caused severe phytotoxicity. Kasgamycil SL streptomycin WP streptomycin·copper hydroxide WP and copper hydroxide WP were potential bactericides which could substitute streptomycin sulfate·oxytetracycline WP. Selective applications of the bactericides according to their optimum spray time can enhance the control efficacies against bacterial canker of kiwifruit and retard the emergency of resistant strains of P. syringae pv. actinidiae to the bactericides. The optimum spray number of streptomycin sulfate·oxytetracycline WP was 3 times with 15-day-intervals or 4 times with 10-day-intervals. The result suggested that the potential bactericides to bacterial canker of kiwifruit should be also used according to their optimum spray schedules in order to get their highest control efficacies.

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Biocontrol of Anthracnose of Chili Pepper by Bacillus sp. NAAS-1 (Bacillus sp. NAAS-1을 이용한 고추 탄저병 생물학적 방제)

  • Yoo, Jae Hong;Park, In Cheol;Kim, Wan Gyu
    • The Korean Journal of Mycology
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    • v.40 no.4
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    • pp.277-281
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    • 2012
  • Bacillus sp. NAAS-1 isolated from the field of Chili pepper was tested for biocontrol activity against anthracnose pathogen of Chili pepper caused by Colletotrichum acutatum. The antifungal activity of Bacillus sp. NAAS-1 culture broth was compared with synthetic fungicide containing carbendazim (40%) and kasugamycin (3.45%). Bacillus sp. NAAS-1 showed a similar fungicidal activity against the anthracnose pathogen at the concentration of 50 ${\mu}L/mL$ in comparison to the fungicide containing carbendazim (40%) and kasugamycin (3.45%) using a cup method. Bacillus sp. NAAS-1 also exhibited its potent fungicidal activity against the anthracnose in vivo test at the concentration of 50 ${\mu}L/mL$ when compared to the fungicide containing carbendazim (40%) and kasugamycin (3.45%).