Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Physiological Characteristics of Immobilized Streptomyces Cells in Continuous Cultures at Different Dilution Rates

  • Kim, Chang-Joon (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology) ;
  • Chun, Gie-Taek (Division of Life Science, Kangwon National University) ;
  • Jeong, Yeon-Ho (Division of Biotechnology, Kangwon National University) ;
  • Lee, Sang-Jong (STR Biotech. Co., Ltd.)
  • Published : 2002.08.01
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Abstract

Physiological characteristics such as specific productivity, morphology of Streptomyces cells Immobilized on celite beads, and operational stability at different dilution rates were investigated in continuous immobilized-cell cultures for the production of kasugamycin. At a dilution rate (D) of 0.05 $h^{-1}$, a relatively high specific productivity was attained and the loss of cell-loaded beads was negligible. At D=0.1 $h^{-1}$, a higher specific productivity and cell concentration could be obtained, resulting in a significantly improved volumetric kasugamycin productivity. However, no stable operation could be maintained due to a significant loss of cell-loaded beads from the reactor that was caused by their fluffy morphology developed in the later stage. At D=0.2 $h^{-1}$, the production of kasugamycin and cell growth were observed to be severely inhibited by the high concentration of residual maltose.

Keywords

References

  1. Biotechnol. Bioeng. v.25 Thienamycin production by immobilized cells of Streptomyces cattleya in a bubble column Arcuri, E. J.;J. R. Nichols;T. S. Brix;V. G. Santamarina;B. C. Buckland;S. W. Drew https://doi.org/10.1002/bit.260251010
  2. Biotechnol. Bioeng. v.28 Continuous production of thienamycin in immobilized cell systems Arcuri, E. J.;G. Slaff;R. Greasham https://doi.org/10.1002/bit.260280611
  3. J. Antibiotics v.45 A complex of novel growth-promoting thiopeptide antibiotics produced by a strain of Streptomyces gardneri. Taxonomy and fermentation studies Boeck, L. D.;D. M. Berry;F. P. Mertz;R. W. A. Wetzel https://doi.org/10.7164/antibiotics.45.1222
  4. Biotechnol. Bioeng. v.37 Periodic operation of immobilized live cell bioreactor for the production of candicidin Constantinides, A.;N. Mehta https://doi.org/10.1002/bit.260371105
  5. Biotechnol. Lett. v.10 Production of actinomycin D with immobilized Streptomyces parvullus under nitrogen and carbon starvation conditions Dalili, M.;P. C. Chau https://doi.org/10.1007/BF01026160
  6. Appl. Microbiol. Biotechnol. v.52 Pharmaceutically active secondary metabolites of microorganisms Demain,A. L. https://doi.org/10.1007/s002530051546
  7. Appl. Microbiol. Biotechnol. v.32 Nutritional control of actinorhodin production by Streptomyces coelicolor A3(2): Suppressive effects of nitrogen and phosphate Doull, J. L.;L. C. Vining https://doi.org/10.1007/BF00903781
  8. Biotechnol. Bioeng. v.36 Optimization of a pellicular biocatalyst for penicillin production by Penicillium chrysogenum Flanagan, W. P.;H. E. Klei;W. D. Sundstrom;C. W. Lawton https://doi.org/10.1002/bit.260360608
  9. Biotechnol. Bioeng. v.25 Confining mycelial growth to porous microbeads: A novel technique to alter the morphology of non-Newtonian mycelial cultures Gbewonyo, K.;D. I. C. Wang https://doi.org/10.1002/bit.260250407
  10. Antimicrob. Agents Chemother. v.32 Involvement of glucose catabolism in avermectin production by Streptomyces avermitilis Ikeda, H.;H. Kotaki;H. Tanaka;S. Omura https://doi.org/10.1128/AAC.32.2.282
  11. Biotechnol. Prog. v.17 Continuous culture of immobilized Streptomyces cells for kasugamycin production Kim, C. J.;Y. K. Chang;C.-T. Chun;Y. H. Jeong;S. J. Lee https://doi.org/10.1021/bp010020k
  12. Biotechnol. Prog. v.16 Enhancement of kasugamycin production by pH shock in batch cultures of Streptomyces kasugaensis Kim, C. J.;C.-T. Chun;Y. K. Chang https://doi.org/10.1021/bp000038f
  13. Biotechnol. Bioeng. v.28 Production of penicillin in a fluidizedbed bioreactor using a carrier-supported mycelial growth Kim, J. H.;D. K. Oh;S. K. Park;Y. H. Park;D. A. Wallis https://doi.org/10.1002/bit.260281211
  14. J. Ind. Microbiol. v.4 Carbon catabolite regulation of rebeccamycin production in Saccharothrix aerocolonigenes Lam, K. S.;J. Mattei;S. Forenza https://doi.org/10.1007/BF01569794
  15. Biotechnol. Prog. v.15 Development of a cell-loaded biosupport separator for continuous immobilized-cell perfusion culture Lee, T. H.;G. -T. Chun;Y. K. Chang https://doi.org/10.1021/bp9900209
  16. Biotechnol. Prog. v.13 Development of sporulation/immobilization method and its application for the continuous production of cyclosporin A by Tolypocladium inflatum Lee, T. H.;G.-T. Chun;Y. K. Chang https://doi.org/10.1021/bp970069j
  17. Microbiol. Rev. v.44 Control of antibiotic biosynthesis Martin, J. F.;A. L. Demain
  18. Biotechnol. Bioeng. v.32 Production of penicillin in a fluidized-bed bioreactor: Control of cell growth and penicillin production by phosphate limitation Oh, D. K.;C. K. Hyun;J. H. Kim;Y. H. Park https://doi.org/10.1002/bit.260320421
  19. Principles of Microbe and Cell Cultivation Chemostat culture Pirt, S. J.(ed.)
  20. Biotechnol. Bioeng. v.53 Continuous production of a hybrid antibiotic by Streptomyces lividans TK21 pellets in a three-phase fluidized-bed bioreactor Sarra, M.;C. Casas;F. Godia https://doi.org/10.1002/(SICI)1097-0290(19970320)53:6<601::AID-BIT8>3.0.CO;2-Q
  21. Japanese patent. J58170494. 07.10.83 High yielding preparation of kasugamycin Umezawa, H.;Y. Yagi