• Proceedings of the PSK Conference
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• 2002.10a
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• pp.333.2-333.2
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• 2002

Kakkalide from Puerariae Flos expresses pharmacological actions after biotransformation to irisolidone by intestinal bacteria. B. breve K-110 was isolated as a bacterium metabolizing kakkalide. Therefore. we purified kakkalide-metabolizing p-Xylosidase from B. breve K-110. ${\beta}$-Xylosidase from B. breve K-110 (isolated from Korean intestinal microflora) was induced by kakkalide. We used defined medium contating 1mM kakkalide for the cultivationof B. breve K-110. (omitted)

• Proceedings of the PSK Conference
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• 2003.04a
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• pp.225.2-226
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• 2003

The inhibitory effect of kakkalide isolated from Puerariae flos on ethanol-induced lethality and hepatic injury were investigated. Intraperitoneally treated Kakkalide was weakly reduced the mortality associated with administration of ethanol and did not reduce alcohol hepatotoxicity. However, orally administered kakkalide and intraperitoneally administered irisolidone significantly reduced serum ALT and AST activities on liver-injured mice by ethanol. (omitted)

• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.535-540
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• 2012

${\beta}$-D-Xylosidase (E.C. 3.2.1.37) from Bifidobacterium breve K-110, which hydrolyzes ginsenoside Ra1 to ginsenoside Rb2, was cloned and expressed in Escherichia coli. The ($His_6$)-tagged recombinant enzyme, designated as XlyBK-110, was efficiently purified using $Ni^{2+}$-affinity chromatography (109.9-fold, 84% yield). The molecular mass of XylBK-100 was found to be 55.7 kDa by SDS-PAGE. Its sequence revealed a 1,347 bp open reading frame (ORF) encoding a protein containing 448 amino acids, which showed 82% identity (DNA) to the previously reported glycosyl hydrolase family 30 of Bifidobacterium adolescentis ATCC 15703. The $K_m$ and $V_{max}$ values toward p-nitrophenyl-${\beta}$-D-xylopyranoside (pNPX) were 1.45mM and 10.75 ${\mu}mol/min/mg$, respectively. This enzyme had pH and temperature optima at 6.0 and $45^{\circ}C$, respectively. XylBK-110 acted to the greatest extent on xyloglucosyl kakkalide, followed by pNPX and ginsenoside Ra1, but did not act on p-nitrophenyl-${\alpha}$-L-arabinofuranoside, p-nitrophenyl-${\beta}$-D-glucopyranoside, or p-nitrophenyl-${\beta}$-D-fucopyranoside. In conclusion, this is the first report on the cloning and expression of ${\beta}$-D-xylosidase-hydrolyzing ginsenoside Ra1 and kakkalide from human intestinal microflora.

• Archives of Pharmacal Research
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• v.26 no.3
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• pp.210-213
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• 2003

Puerariae Flos is a traditional herbal medicine that has long been used as a treatment for colds, diabetes, and hangovers. The herbal medicine contains a wide variety of isoflavones such as kakkalide, tectoridin, and tectorigenin. This study demonstrates that the substances undergo a certain degree of change depending on the storage period by the method of HPLC and $^{13}C-NMR$, and that the HPLC analysis can be used to determine the freshness of Puerariae Flos.