• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.483-493
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• 2010

Knock-out of a gene by insertional mutagenesis is a direct way to address its function through the mutant phenotype. Among ca. 15,000 gene-trapped Ds insertion lines of rice, we identified one line from selected sensitive lines in highly salt stress. We conducted gene tagging by TAIL-PCR, and DNA gel blot analysis from salt sensitive mutant. A gene encoding an oligopeptide transporter (OPT family) homologue was disrupted by the insertion of a Ds transposon into the OsOPT10 gene that was located shot arm of chromosome 8. The OsOPT10 gene (NP_001062118.) has 6 exons and encodes a protein (752 aa) containing the OPT family domain. RT-PCR analysis showed that the expression of OsOPT10 gene was rapidly and strongly induced by stresses such as high-salinity (250 mM), osmotic, drought, $100\;{\mu}M$ ABA. The subcellular localization assay indicated that OsOPT10 was localized specifically in the plasma membrane. Overexpression of OsOPT10 in Arabidopsis thaliana and rice conferred tolerance of transgenic plants to salt stress. Further we found expression levels of some stress related genes were inhibited in OsOPT10 transgenic plants. These results suggested that OsOPT10 might play crucial but differential roles in plant responses to various abiotic stresses.

• Korean Journal of Agricultural Science
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• v.28 no.1
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• pp.33-40
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• 2001

The authors attempted to derive a comprehensive quantitative structure-toxicity relationships (QSTRs) between various physicochemical parameters of phenyl substituents in fungicidal phenylthionocarbamate derivatives and toxicity evaluated using TOPKAT calculation. On the basis of this approach we made preditions for toxicity values for not yet tested substances with respect to these systems. The results suggested that the optimal values, $(B_2)_{opt.}=1.54_{\AA}$(Ames mutagenicity), $(R)_{opt.}=0.16$ (car-cinogenicity of male rat), $(\pi)_{opt.)=0.16$ (carcinogenicity of male mouse), $({\varepsilon}LOMO)_{opt}=-0.52e.v.$ ($LD_{50}$ of rat oral), $(B_3){opt.}=1.54_{\AA}$(chronic LOAEU), $(logP)_{opt.}=4.25$ ($LC_{50}$ of Fathead minnow) and $({\sigma})_{opt}=-0.68$ ($EC_{50}$ of Daphnia magna) of phenyl substituents were strongly correlated with the acute and chronic toxicities.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.4
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• pp.249-257
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• 2015

This paper introduces a heuristic algorithm to NP-hard travelling salesman problem. The proposed algorithm, in its bid to determine initial path, applies SW-DNN, DW-DNN, and DC-DNN, which are modified forms of the prevalent Double-sided Nearest Neighbor Search and searches the minimum value. As a part of its optimization process on the initial solution, it employs 2, 2.5, 3-opt of a local search k-opt on candidate delete edges and 4-opt on undeleted ones among them. When tested on TSP-1 of 26 European cities and TSP-2 of 49 U.S. cities, the proposed algorithm has successfully obtained optimal results in both, disproving the prevalent disbelief in the attainability of the optimal solution and making itself available as a general algorithm for the travelling salesman problem.

• The Journal of Industrial Distribution & Business
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• v.11 no.2
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• pp.49-59
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• 2020

Purpose: Online and social media and mobile shopping are increasing and companies are required to provide personal information in order to supplement the non-invasive characteristics of the channels. With the increased provision of personal information, consumers' personal and social concerns about the prevention of personal information infringement are also increasing, and in response, personal or opt-in marketing has emerged to compensate for reckless information abuse. Despite the background of this emergence, the existing prior studies are limited to ignoring the negative feelings of consumers in the real world, including only the net function and positive effect of the opt-in mail. Research design, data and methodology: The research framework was intended to utilize the impact of human marketing activities on consumer attitudes combined with positive and negative factors. Factors that positively affect attitudes toward permation marketing were presented, such as informality, and perceived risks were presented as negative impact factors. Also, based on previous prior research, the prior factors of opt-in marketing were to present the effect on purchase intent through the medium of attitude toward opt-in marketing. Results: In this study, we used the framework of a two factor theory to address positive and negative factors as a leading factor in the customer attitude toward opt-in mail advertising, and as a result, functionality and personalization have a positive effect on customer attitude and perceived risk have a negative impact on customer attitude. In addition, it was confirmed that the customer attitude formed this way affects the intention to purchase again. Conclusions: This study suggests that we have demonstrated that marketing, an opt-in marketing that has been recognized as part of marketing that is deployed after obtaining customer consent, has been applied without any other marketing methodology. E-mail advertising at this point also provides practical implications that the system safeguards are in place under an opt-in protocol or system, and that even if an e-mail advertisement is carried out, customers will need to look at the level of awareness about the risks, and suggests that they need to consider the customer's journey that could lead to purchase at the content level.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.296-302
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• 2017

Rice (Oryza sativa) cultivars show an impairment of growth and development in response to abiotic stresses such as drought, salinity, heat and cold at the early seedling stage. The tolerance to heat stress in plants has been genetically modulated by the overexpression of heat shock transcription factor genes or proteins. In addition to a high temperature-tolerance that has also been altered by elevating levels of osmolytes, increasing levels of cell detoxification enzymes and through altering membrane fluidity. To examine the heat tolerance in transgenic rice plants, three OsOPT10 overexpressing lines were characterized through a physiological analysis, which examined factors such as the electrolyte leakage (EL), soluble sugar and proline contents. We further functionally characterized the OsOPT10 gene and found that heat induced the expression of OsOPT10 and P5CS gene related proline biosynthesis. It has been suggested that the expression of OsOPT10 led to elevated heat tolerance in transgenic lines.

• Journal of the Korea Society of Computer and Information
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• v.17 no.10
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• pp.155-165
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• 2012

This paper suggests traveling salesman problem algorithm that have been unsolved problem with NP-Hard. The proposed algorithm is a heuristic with edge-swap method. The classical method finds the initial solution starts with first node and visits to mostly adjacent nodes then decides the traveling path. This paper selects minimum weight edge for each nodes, then perform Min-Min method that start from minimum weight edge among the selected edges and Min-Max method that starts from maximum weight edges among it. Then we decide tie initial solution to minimum path length between Min-Min and Min-Max method. To get the final optimal solution, we apply previous two-opt to initial solution. Also, we suggest extended 3-opt and 4-opt additionally. For the 7 actual experimental data, this algorithm can be get the optimal solutions of state-of-the-art with fast and correct.

• The korean journal of orthodontics
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• v.21 no.3
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• pp.581-590
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• 1991

This study was carried out for the comparison of the head postures between mouth breathers and normal nasal breathers. For this purpose, a test group of 28 mouth breathers, between the ages of 8 to 12 years old and a control group of 25 nasal breathers were used. Following results were obtained after comparing the craniofacial and craniocervical angulations to the True Vertical line. 1. In the comparisions relating to the True Vertical Line, results were obtained using four items; NSL/VER, FH/VER, NL/VER, PMV/VER. Variations were observed in all the items and the craniofacial angulations in the mouth breathers group showed more superior position compared to the control group. 2. In the comparisions relating to the craniocervical angulations, results were obtained using four items; NSL/OPT, FH/OPT, NL/OPT, PMV/OPT. Variations between the two groups were observed only in the PMV/OPT item and the mouth breather group showed more craniocervical angulations compared to the control group.

• Journal of the Korea Society of Computer and Information
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• v.20 no.5
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• pp.31-39
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• 2015

The degree-constrained minimum spanning tree (d-MST) problem is considered NP-complete for no exact solution-yielding polynomial algorithm has been proposed to. One thus has to resort to an heuristic approximate algorithm to obtain an optimal solution to this problem. This paper therefore presents a polynomial time algorithm which obtains an intial solution to the d-MST with the help of Kruskal's algorithm and performs k-opt on the initial solution obtained so as to derive the final optimal solution. When tested on 4 graphs, the algorithm has successfully obtained the optimal solutions.

• Journal of Oral Medicine and Pain
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• v.21 no.2
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• pp.305-315
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• 1996

This study was performed to investigate the changes of head posture according to natural standing or sitting posture. Twenty seven healthy dental students without any signs and symptoms of temporomandibular disorders participated in this study. Cervical resting posture (CRP) of the head in sagittal plane was measured by Cervical-Range-of-Motion $^\textregistered$(CROM, U.S.A.) and lateral cephalograph was taken in natural posture. The items related to angle in cephalograph were the angles of cranial and cervical inclination to true vertical line(VER/NSL, VER/AML), the angles of cervical inclination to nasion-sella line(CVT/NSL, OPT/NSL), the angles of comical inclination to horizontal line(CVT/HOR, OPT/HOR), the angle of cervical lordosis(CVT/OPT). The items related to line measurement were the distance from subocciput to Cl(Dl), Cl to C2(D2), C2 to C3(D3), C3 to C4(D4), the upper(PNS to posterior pharyngeal wall) and the lower(tongue base to posterior pharyngeal wall) pharyngeal space, the distance from nation to mention(Na-Me), and the radius of comical curvature from the first comical vertebra(Cl ) to the fifth cervical vertebra(C5). The data were analyzed with SAS/STAT program. The obtained results were as follows : 1. Most items related to angular measurement showed significant difference between in standing and sitting posture. The angles of CRP, CVT/NSL, OPT/NSL, CVT/HOR, OPT/HOR, and CVT/OPT were high in sitting posture, but the angles of VER/NSL, VER/NSL were low in sitting posture. 2. In vertebral distance, only the distance between C3 and C4 was differed by the posture, which decreased in sitting posture. In sitting posture, the distance from nasion to menton(Na-Me) was longer, but the radius was shorter than in standing posture. 3. Correlationship in angular measurements was almost same in both postures. Ceervical resting posture(CRP) was correlated with VER/NSL, VER\ulcornerNSL was correlated with CRP, CVT/NSL, and OPT/NSL, VER/AML was correlated with CVT/HOR, OPT/HOR, CVT/OPT, and the angle of cervical lordosis(CVT/OPT) was correlated with the radius. 4. Correlationship in linear measurement was observed only in among D3, D4, and radius. And the Na-Me was not correlated with any other items. From this results, The author concluded that the head posture in sitting was mote backward extended than in standing.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.47 no.4
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• pp.13-20
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• 2015

Soda-AQ pulp made from sugarcane bagasse (SCB) and oil palm trunk (OPT) were bleached in element chlorine free (ECF) sequence. Bleached SCB and OPT pulp was achieve higher brightness than 85.0% ISO. Viscosity of SCB bleached pulp and OPT bleached pulp were achieved 18-27 cPs and 18-26 cPs. In 7.8% active chlorine dioxide addition, bleached SCB pulp was shown 88.7% ISO brightness and 20.4 cPs viscosity. And bleached OPT pulp was shown 88.5% ISO brightness and 18.8 cPs viscosity with 7.8% active chlorine dioxide addition.