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Evaluation of Genetic Variation and Phylogenetic Relationship among North Indian Cattle Breeds

  • Sharma, Rekha;Pandey, A.K.;Singh, Y.;Prakash, B.;Mishra, B.P.;Kathiravan, P.;Singh, P.K.;Singh, G.
    • Asian-Australasian Journal of Animal Sciences
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    • v.22 no.1
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    • pp.13-19
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    • 2009
  • In the present study, genetic analyses of diversity and differentiation were performed on four breeds of Indian zebu cattle (Bos indicus). In total, 181 animals belonging to Ponwar, Kherigarh, Gangatiri and Kenkatha breeds were genotyped for 20 cattle specific microsatellite markers. Mean number of alleles observed per locus (MNA) varied between 5.75 (Kenkatha) to 6.05 (Kherigarh). The observed and expected heterozygosity for the breeds varied from 0.48 (Gangatiri) to 0.58 (Kherigarh) and 0.65 (Kenkatha) to 0.70 (Kherigarh), respectively. $F_{IS}$ estimates of all the breeds indicated significant deficit of heterozygotes being 28.8%, 25.9%, 17.7% and 17.7% for Gangatiri, Ponwar, Kherigarh and Kenkatha, respectively. The $F_{ST}$ estimates demonstrated that 10.6% was the average genetic differentiation among the breeds. Nei's genetic distance DA and Cavalli- Sforza and Edwards Chord distance ($D_C$) and the phylogenetic tree constructed from these reflected the close genetic relationship of Gangatiri and Kenkatha, whereas Ponwar appears to be more distant.

Limiting the Number of Open Projects to Shorten the NPD Schedule

  • Wang, Miao-Ling;Yang, Chun-I;Chang, Sheng-Hung
    • Industrial Engineering and Management Systems
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    • v.10 no.1
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    • pp.34-42
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    • 2011
  • Many companies open multiple projects simultaneously due to market trends, which results in a crowding out effect because of limited resources. R&D engineers become overloaded and scheduling of product development is delayed resulting in timing misses and lost sales leads. The company in this case study (Company A), often opens up many projects simultaneously in order to respond to market needs quickly. The engineers are overloaded and, of course, the schedule is delayed. In order to identify problems, Company A began using Dr. Goldratt's Thinking Processes (TP) during new product development (NPD). When the analysis phase of TP was completed, Company A's core problem was identified as "the quantity of kick-off projects." Consequently, new rules and conditions and procedures were proposed for the opening, suspending, stopping, and closing of projects. Finally, the "Future Reality Tree" ensured that the proposed rules, conditions and procedures were set up as an available solution approved for practical application by executives. After a one-year trial run, the results showed that the Project Duration Rate was reduced by 53%, the Project Closed Rate was increased by 140% and the Project on Time Rate was increased from 10% to 68%. The above results give significant evidence of the benefits of the proposed methodology.

Text-independent Speaker Identification by Bagging VQ Classifier

  • Kyung, Youn-Jeong;Park, Bong-Dae;Lee, Hwang-Soo
    • The Journal of the Acoustical Society of Korea
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    • v.20 no.2E
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    • pp.17-24
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    • 2001
  • In this paper, we propose the bootstrap and aggregating (bagging) vector quantization (VQ) classifier to improve the performance of the text-independent speaker recognition system. This method generates multiple training data sets by resampling the original training data set, constructs the corresponding VQ classifiers, and then integrates the multiple VQ classifiers into a single classifier by voting. The bagging method has been proven to greatly improve the performance of unstable classifiers. Through two different experiments, this paper shows that the VQ classifier is unstable. In one of these experiments, the bias and variance of a VQ classifier are computed with a waveform database. The variance of the VQ classifier is compared with that of the classification and regression tree (CART) classifier[1]. The variance of the VQ classifier is shown to be as large as that of the CART classifier. The other experiment involves speaker recognition. The speaker recognition rates vary significantly by the minor changes in the training data set. The speaker recognition experiments involving a closed set, text-independent and speaker identification are performed with the TIMIT database to compare the performance of the bagging VQ classifier with that of the conventional VQ classifier. The bagging VQ classifier yields improved performance over the conventional VQ classifier. It also outperforms the conventional VQ classifier in small training data set problems.

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Development of Functional Yogurts Prepared with Mulberries and Mulberry Tree Leaves

  • Lee, An-Cheol;Hong, Youn-Ho
    • Food Science of Animal Resources
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    • v.30 no.4
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    • pp.649-654
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    • 2010
  • In order to develop new functional yogurts using mulberries and mulberry leaves, which were cultivated in Hwasun-gun, Jeonnam Province, Korea, the nutritional compositions, fermentation conditions, sensory properties, and storage stabilities of the yogurts were analyzed. The mulberry powder yogurt contained 87.96% moisture, 3.21% carbohydrate, 4.52% protein, 3.63% lipid, and 0.68% ash, and the mulberry leaf yogurt contained 86.36% moisture, 4.13% carbohydrate, 4.87% protein, 3.79% lipid, and 0.85% ash. A yogurt base was fermented for 13 h with 0.01% ABT-5 starter inoculum at $40^{\circ}C$. To prepare the mulberry jam and mulberry leaf yogurts, a variety of mulberry jam and mulberry leaf samples were added to the yogurt base. The sensory evaluation results of the yogurts containing the mulberry jam and mulberry leaves indicated that a product made with 15% mulberry jam was more strongly preferred than other samples. When the mulberry jam and mulberry leaf yogurts were stored at $4^{\circ}C$ for 15 d, there were no significant changes in pH, titratable acidity, or viable cell numbers of lactic acid bacteria and Bifidobacterium bifidum.

Morphological and Ecological Study of Gastrolina depressa Baly (Coleoptera: Chrysomelidae) (호두나무잎벌레(Gastrolina depressa)의 형태적 및 생태학적 특성)

  • Chang, Seok-Jun;Park, Il-Kwon
    • Korean journal of applied entomology
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    • v.50 no.3
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    • pp.253-256
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    • 2011
  • The walnut leaf beetle, Gastrolina depressa, belongs to family Chrysomelidae (Coleoptera), and is one of the major pests of walnut trees. G. depressa eggs were oval and dark orange. The three G. depressa larval instars were gray in color. The larval period was approx. 8.14 days ($24^{\circ}C$, 16L:8D). Adults of both sexes were dark blue, and females were larger than males. Overwintered adults emerged in late April, and laid eggs in clusters on young leaves of the host tree. Mature 3rd instar larvae pupated after a short prepupal period. Adults emerged starting mid-May and entered into diapause shortly afterward.

Fine-scale initiation of non-native Robinia pseudoacacia riparian forests along the Chikumagawa River in central Japan

  • Kurokochi, Hiroyuki;Hogetsu, Taizo
    • Journal of Ecology and Environment
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    • v.37 no.1
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    • pp.21-29
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    • 2014
  • Robinia pseudoacacia has become invasively naturalized in Japan. We investigated the role of sexual reproduction in the development of R. pseudoacacia riparian forests along the Chikumagawa River in Japan, by using five chloroplast (cpSSR) and seven nuclear (nSSR) markers. We identified eight chloroplast haplotypes and 147 nuclear genotypes from 619 R. pseudoacacia trees sampled in three plots (Plots A, B, and C) and along two line transects (Lines D and E). CpSSR analyses showed that multiple maternal lines were distributed along the river, and that some haplotypes from different populations overlapped. In addition, while Plots A and B were separated by a short distance, only these two plots exhibited genetic differentiation in the haplotypes. In the nSSR analysis, all pairwise $F_{ST}$ values among the three plots were significantly different from zero. Kinship analysis based on nSSR markers revealed that kinship connected many individuals to another individual from the same plot. These results indicate that seed dispersal near to mother trees contributes to the fine-scale genetic structure of R. pseudoacacia riparian forests. Our results indicate that sexual reproduction, in addition to asexual reproduction, is a major contributor to the fine-scale formation of R. pseudoacacia forests.

Growth and Morphological Charactersistics of Soybean Sprouts Treated with Leaf Extracts of Thea sinensis L. and Eucommia ulmoides Oliver (녹차와 두충 잎 추출물 처리에 따른 콩나물의 생장 및 형태적 특징)

  • Kang, Jin-Ho;Park, Cheol-Jong;Yoon, Soo-Young;Jeon, Seung-Ho;Her, Chang-Young
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.1
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    • pp.11-16
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    • 2005
  • Soybean sprouts presoaked to leaf extracts were on sale as taking a growing concern about wellbeing foods. The study was carried out to compare the effects of culture period after presoaking tea (Thea sinensis L.) and hard rubber tree (Eucommia ulmoides Oliver) leaf extracts on lateral root formation, growth and morphological characters of the sprouts. Seeds of three cultivars, Pungsannamulkong, Sowonkong and Junjery, were imbibed for 5 hours into 10% solutions immediately before 5, 6 and 7 day cultures. The cultured sprouts were classified into 4 categories to calculate their composition rates on the base of hypocotyllengths; >7 cm (A),4 to 7 cm (B),< 4 cm (C) and not germinated (D), and their morphological characters, fresh and dry weights were measured. Rate of A was the lowest in Junjery of the three cultivars, while that of C showed reverse result compared to A. Similar results to that of A were observed between the three cultivars in sprout length including hypocotyl and root, diameters in middle and hook parts of hypocotyl, total fresh and dry weights. Regardless of tea and hard rubber tree leaf extract treatments, longer culture periods increased rate of A due to decrements of B, C and D, and also did the growth and the morphological characters mentioned above. Although longer culture periods reduced the increment of lateral roots, the latter treatment was more delayed in their formation and less in the roots per sprout than the former one. The growth and the morphological characters above were less in the latter treatment than in the former one in general, meaning that hard rubber tree leaf extract could more repress growth of soybean sprouts than tea leaf extract.

Developing Landscape Analysis Method for Forest Fire Damaged Area Restoration Using Virtual GIS (Virtual GIS를 이용한 산불피해지 복구 경관분석기법 개발)

  • Jo, Myung-Hee;Lee, Myung-Bo;Kim, Joon-Bum;Lim, Ju-Hun;Kim, Sung-Jae
    • Journal of the Korean Association of Geographic Information Studies
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    • v.7 no.1
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    • pp.75-83
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    • 2004
  • In Korea the number of forest fire occurrence and its damaged area have increased drastically and the plans for afforestation such as sound erosion control restoration and forestation have performed to restore for forest fire damaged area. In this study fire resistant forest was developed by selecting fire resistance tree species and applying GIS analysis, considering the characteristic of forest fire and location environment in forest fire damaged area along the east coast. Moreover, it showed the possibility of how spatial information technology such as virtual GIS could be applied during restoring forest fire damaged area and approaching landscape ecology researches. Especially the fire resistant forest was established by using GIS analysis against large scaled forest fires then the best forest arrangement was performed through this fire resistant forest species and 3D modeling in study area. In addition, the forest landscape was established through site index on passing years and then 3D topography and tracking simulation, which is very similar to real world, were constructed by using virtual GIS.

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A Survey on House Sparrow Population Decline at Bandel, West Bengal, India

  • Ghosh, Samik;Kim, Ki-Hyun;Bhattacharya, R.
    • Journal of the Korean earth science society
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    • v.31 no.5
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    • pp.448-453
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    • 2010
  • The population of House sparrow (Passer domesticus), once a very common bird, has declined markedly in most parts of the world including India. Sparrows were distributed widely in the district Hoogly, West Bengal, India. However over the past few decades, they became not so common in this part of the wo rld. A study has been conductedto establish the database for their current population and to assess the possible causes of their decline at Bandel ($22^{\circ}$52'22''N/$88^{\circ}$22'53''E) placed beside the river Hoogly from September 2008 to May 2009. After prolonged searching, a relatively dense population of sparrow (total subject 270) was found in busy areas of railway station and a nearby market in Bandel. The behavior of these birds was studied extensively from early morning to late night. The noise level of the area varied from 35 to 95 dB with an average of 70.6 dB from 04:30 am-10:30 pm, IST. It was also observed that the sparrows took rest on the site of the tree where the illumination level was low (30-45 lux). Sparrows residing at the Bandel station are habituated with the loud noise, being undisturbed by passing trains. Thus, it can be concluded that in spite of heavy noise of trains, crowdy travelers, and lack of nest sites, they remain at the station because of availability of food in the nearby roadside market. Based on this observation, sound pollution and availability of food are not responsible for their decline.

Validation of Morphology-based Identification of Two Cynoglossidae Larvae using Mitochondrial DNA (참서대과(Pisces: Cynoglossidae) 자어 2종의 미토콘드리아 DNA에 의한 형태동정의 타당성)

  • Kwun, Hyuck-Joon;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.43 no.5
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    • pp.482-488
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    • 2010
  • Three specimens of Cynoglossidae larvae were collected from the southern Korean Sea in May and August of 2009, and were identified using morphological and molecular analysis. Specimens were divided into two groups based on the number of elongated dorsal fin rays on the top of the head: Cynoglossidae sp. A was defined as having two elongated dorsal fin rays, while Cynoglossidae sp. B possessed a single elongated dorsal fin ray. One specimen of Cynoglossidae sp. A, a post-larva with a notochord length (NL) of 5.8 mm was thought to be a Cynoglossus joyneri larva based on the presence of 115 dorsal pterogiophores, 85 anal pterogiophores, and 50 myomeres. Two specimens of Cynoglossidae sp. B, a 4.1 mm NL larva and a 11.3 mm NL juvenile, were thought to be Cynoglossus abbreviatus based on the presence of yolk in the former and 133 dorsal fin rays, 105 anal fin rays, and 63 myomeres in the latter. To test this morphology-based identification, molecular analysis was conducted using 419-422 bp of mitochondrial DNA 16S rRNA. Cynoglossidae sp. A was clearly matched to a Cynoglossus joyneri adult (d=0.000) and Cynoglossidae sp. B clustered closely with Cynoglossus abbreviatus adults (d=0.002). A neighbor-joining tree supported this robust relationship (bootstrap value=100%). Therefore, these molecular data validate the morphological identification of the two Cynoglossidae larval species.