• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.19 no.7
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• pp.32-38
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• 2005

A hybrid discharge type ozonizer, which is superposed silent and surface discharges, has been designed and manufactured to apply for Nitrogen Oxides(NO) gas removal. The ozonizer consists of three electrodes, and is classified three types of ozonizer by changing applied voltage. Investigation was carried out variance with the flow rate of supplied oxygen gas, discharge power and the sorts of superposed discharge type ozonizer. Moreover, NO(1200[ppm])/$N_2$ gas removal investigation was also conducted to apply for environment improvement field. Two kinds of NO gas removal investigations were conducted. It distinguishes the investigations into NO gas reaction method. According to these studies, maximum removal rate of 100[%] in NO gas was obtained, and 8334[ppm] and 3249[mg/h] of maximum ozone concentration and generation were also obtained respectively.

• Journal of the Korean Society of Safety
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• v.35 no.2
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• pp.111-117
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• 2020

When KS C IEC 60069-10-1(2015) standard is applied to estimate a hazardous area, the chart showing the relationship between a hazardous area distance and release characteristic is used as a guide to determine the extent of hazardous zones for various forms of release. Three release characteristic lines based on the three types of release as an unimpeded jet release with high velocity, a diffusive jet release with low velocity, and a release of heavy gases or vapours that spread along horizontal surfaces are given. As these characteristic lines have the low limit threshold, it is difficult to estimate the hazardous area distance when the value of release characteristic is under the low limit threshold. And KS C IEC 60079-10-1(2015) standard shows the concept for a zone of Negligible extent(NE) which can be considered as non hazardous area, but it is also difficult to apply the concept of a Negligible extent. The purpose of this paper is to suggest the guideline for the release characteristic to decide a hazardous area distance and the Negligible extent(NE) being considered as non-hazardous area when deciding a hazardous area distances by the KS C IEC 60079-10-1 standard.

• Journal of Microbiology and Biotechnology
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• v.30 no.1
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• pp.109-117
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• 2020

Cre recombinase is widely used to manipulate DNA sequences for both in vitro and in vivo research. Attachment of a trans-activator of transcription (TAT) sequence to Cre allows TAT-Cre to penetrate the cell membrane, and the addition of a nuclear localization signal (NLS) helps the enzyme to translocate into the nucleus. Since the yield of recombinant TAT-Cre is limited by formation of inclusion bodies, we hypothesized that the positively charged arginine-rich TAT sequence causes the inclusion body formation, whereas its neutralization by the addition of a negatively charged sequence improves solubility of the protein. To prove this, we neutralized the positively charged TAT sequence by proximally attaching a negatively charged poly-glutamate (E12) sequence. We found that the E12 tag improved the solubility and yield of E12-TAT-NLS-Cre (E12-TAT-Cre) compared with those of TAT-NLS-Cre (TAT-Cre) when expressed in E. coli. Furthermore, the growth of cells expressing E12-TAT-Cre was increased compared with that of the cells expressing TAT-Cre. Efficacy of the purified TAT-Cre was confirmed by a recombination test on a floxed plasmid in a cell-free system and 293 FT cells. Taken together, our results suggest that attachment of the E12 sequence to TAT-Cre improves its solubility during expression in E. coli (possibly by neutralizing the ionic-charge effects of the TAT sequence) and consequently increases the yield. This method can be applied to the production of transducible proteins for research and therapeutic purposes.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.9
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• pp.103-109
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• 2003

The development of a pulse gun used for the combustion stability rating test(SRT) has been domestically tried out since the SRT should be accompanied in the process of the development of LREs. At the beginning of the development, a rupture problem of the pulse gun body had been solved by changing its material and increasing its thickness. Also, the sealing test was undertaken for a pulse gun equipped with a membrane before conducting a explosion test and by using a specially designed jig after a explosion test. Since it is appropriate to reuse the holder of a pulse gun cavity for an actual SRT, a series of experiments for several variants has been taken to make it reusable. After all, the solution for a reusable holder of a pulse gun has been found and applied for complementing its specification.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.6
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• pp.2463-2469
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• 2011

The failure of display modules in cellular phones due to drop impact depends on the materials, stiffnesses and shapes of the phones. In the early development stage of cellular phones, the behavior of the display modules due to drop impact is tested using jigs where the display modules are installed. In this study, jigs of cellular phones for drop impact test of OLED modules were designed. The material, thickness and number and orientation of ribs were considered as design variables. Jigs were designed in a way that responses of modules due to drop impact were similar to those of modules in cellular phones. The maximum principal stresses at the upper glasses of modules were considered as responses, which were analyzed using a commercial software ABAQUS/Explicit. The drop impact analysis at the height of 1.5 meters showed that the responses of OLED module in the developed jig were in a good agreement with those in cellular phone.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.21 no.7
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• pp.68-74
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• 2007

For carnation productivity improvement, compact-low cost discharge lamp type ozonizer using pulse power source is designed and manufactured. This paper describes the discharge characteristics and the ozone generation characteristics with variation of the output voltage of pulse power source, the quantity of flowing air and the operating number of ozonizer. The important conclusions are as follows. The discharge voltage and current values are proportional to the output voltage of pulse power source. The ozone concentration and generation are proportional to the output voltage of pulse power source and the number of ozonizer. The maximum value of the ozone concentration and generation of the discharge lamp type ozonizer can be obtained 890[ppm] and 59.7[mg/h] respectively. In the carnation productivity house, when the discharge lamp type ozonizer is used the carnation is good quality in the uniformity of the carnation stalk thickness and increased the productivity through improvement of the air and the soil.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.5A
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• pp.451-459
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• 2007

RFID it reads information which is it writes, the semiconductor chip for and the radio frequency system which uses the hazard antenna it has built-in transmission of information it talks. Formation which is transmitted like this collection and America which it filtrates wey the RFID search service back to inform the location of the server which has commodity information which relates with an object past record server. The hazard where measurement analysis result the leader for electronic interference does not occur consequently together from with verification test the power level which is received from the antenna grade where it stands must maintain minimum -55dBm and the electronic interference will not occur with the fact that, antenna and reel his recognition distance the maximum 7m until the recognition which is possible but smooth hazard it must stand and and with the fact that it will do from within and and and 3-4m it must be used Jig it is thought.

• Journal of the Korea Society for Simulation
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• v.15 no.3
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• pp.93-101
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• 2006

Recently, work-related musculoskeletal disorders (WMSDs) become a hot issue in the industrial fields. To prevent the potential risk of workers, various approaches have been adopted. One of the approaches is to improve the design of product, that of jig (or fixture) and that of workstation in the early stage of the development. 3D simulation technology is known as the powerful method for detecting such problems before constructing the workstation, because it is possible to evaluate the posture of worker using 3D models in a cyber space. It enables to find the unexpected problems and save the time and cost for redesign and rework. This paper introduces a 3D simulation case study of workers in an excavator factory. 3D models of products, jigs were developed with CATIA. The assembly processes were animated in IGRIP and DPM. Finally the various postures of worker were simulated using Human. As a result, some postures were analysed as the risky jobs and the result of simulation was used to improve the system.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.4
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• pp.265-273
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• 2014

Wave-body interaction is simulated using a developed code based on the flux-difference splitting scheme for immiscible and incompressible fluids and the hybrid Cartesian/immersed boundary method. A free surface is captured as a moving contact discontinuity within a fluid domain and an approximated Riemann solver is used to estimate the inviscid flux across the discontinuity. Immersed boundary nodes are identified inside an instantaneous fluid domain near a moving body, then dependent variables are reconstructed at those immersed boundary nodes based on interpolation along local normal lines to the boundary. Free surface flows around an oscillating cylinder are simulated and the computed wave elevations are compared with other reported results. The generation of a solitary wave by a moving wave-maker is simulated and the time histories of wave elevations at two different points are compared with other results. The developed code is applied to simulate body motion of an elastically mounted circular cylinder as a solitary wave passes the body. The force acting on an elastically mounted cylinder is compared with the force acting on a fixed cylinder. Grid independency of the computed body motion is established based on a comparison of results using three different-size grids.

• Journal of the Society of Naval Architects of Korea
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• v.54 no.1
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• pp.34-42
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• 2017

In rough seas, green water shipped on board may impose quite large impact loads on the structures on deck and sometimes result in structure damages. One of the essential tasks of the naval fluid engineers is to provide the design impact loads which are needed for proper design of the structure strength against the green water impacts. Computation of the design impact load due to green water needs first a process to find the sea condition and the ship cruising condition which cause maximum green water impacts on structures as well as other succeeding processes to compute ship motion responses, green water flows and impact loads. Also, as a bold and practical process, it is needed that the irregular real seas are to be substituted by design regular waves which are equivalent in view points of green water impacts. In this paper, the whole processes to compute the design green water loads acting on bow structure are set up creatively. And the green water design impact loads acting on the box-type structure of a high-speed ship's bow are computed and discussed.