• ALGAE
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• 제29권1호
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• pp.1-13
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• 2014

The genus Erythroglossum is characterized by Phycodrys-type apical organization, Polyneura-type procarp, and the presence of a midrib. We collected an unidentified Delesseriaceaen species from deep water off the southern coast of the Korean Peninsula. This alga resembles Polyneura japonica in terms of having broadly flattened thalli with a cylindrical stipe, the presence of a midrib and alternative lateral veins. To confirm the taxonomic status of this entity, we compared the morphological features and rbcL sequences among other species of Erythroglossum and P. japonica. As a result, we assigned the new species, Erythroglossum hyacinthinum, to the genus Erythroglossum because of the presence of a midrib. This species is characterized by an elliptical to obovate blade with a short cylindrical stipe, a conspicuous midrib and alternate veins, margins with numerous microscopic dentations, di-trichotomously branching, and bulish-violet iridescence. The phylogeny of rbcL sequences indicates that E. hyacinthinum is definitely a separate entity, but the genera in the tribe Phycodryeae have inconsistent phylogenetic relationships. This is the first study comparing the molecular phylogeny within the genus Erythroglossum.

• 대한본초학회지
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• 제25권4호
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• pp.129-135
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• 2010

Objectives : The present study was undertaken to determine whether the water extract of Achyranthis Radix, which is the roots of Achyranthes japonica (Achyranthis Radix, AR), is efficacious against collagen-induced arthritis (CIA) in DBA/1J mice. Methods : Mice were immunized with bovine type II collagen and orally treated with AR-W (50 and 100 mg/kg/bw) from days 21 to 42 after immunization. Arthritis was evaluated by arthritic score, histological examination of knee joint and serological markers such as TNF-${\alpha}$, $PGE_2$ and anti-type II collagen (C II)IgG. Results : The results showed that comparing with untreated CIA mice, treated with AR-W significantly suppressed the clinical score and joint tissue pathological damages, reduced the serum levels of TNF-${\alpha}$, $PGE_2$ and anti-C II IgG in CIA-mice. These results suggest that AR-W can effectively alleviate inflammatory response on CIA, and anti-inflammatoy of AR-W can be attributed, at least partially, to the inhibition of inflamamtory mediators, $PGE_2$ and TNF-${\alpha}$, in CIA. Conclusions : This study suggests that AR-W has a therapeutic potential in inflammatory joint diseases such as rheumatoid arthritis.

• 한국조경학회지
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• 제26권2호
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• pp.15-27
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• 1998

The purposes of this study were to investigate vegetation structure and present management planning of mountain type green space using the green space changes during the 20 years, actual vegetation, and plant community structure in land area of Inchon, Korea. The actual vegetation area in survey sites was consisted of Quercus acutissima community, Robinia pseudoacacia forest, Pinus rigida forest, Q. mongolica-Pinus rigida community, P. rigida-Q. mongolica community, Q. monogolica community and so on. According to the classification by TWINSPAN, 61 survey plots were divided into 9 groups; Q. mongolica-Alnus japonica-R. pseudoacacia-P. densiflora, R. pseudoacacia-Styrax japonica, P. rigida-R. pseudoacacia-Q. mongolica, R. pseudoacacia-P. rigida-Q. mongolica-A. hirusta, Q. mongolica-P. thunbergii, and prunus sargentii-Zelkova serrata community. From this result, ecological succession trend of vegetation seems to be change from artificial result, ecological succession trend of vegetation seems to be change from artificial planting forest to native plant community which was dominated by Quercus spp.. This study area need to manage for the increase of biodiversity through the restoration of naturalness by ecological management of artificial planting forest and ecological planting of injured green space.

• Natural Product Sciences
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• 제9권1호
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• pp.44-48
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• 2003

The effect of aqueous extract of Stachys riederi var. japonica Miq. (Labiatae) (SRAE) on the immediate-type allergic reactions was investigated. SRAE was found to exhibit a inhibitory activity on the compound 48/80-induced systemic anaphylaxis in mice. SRAE inhibited the plasma histamine release induced by compound 48/80 in mice. In addition, SRAE also inhibited the passive cutaneous anaphylaxis reaction induced by IgE/anti-IgE in mice. The effect of SRAE on the histamine release from rat peritoneal mast cells (RPMC) was studied. SRAE inhibited the histamine release induced by compound 48/80 in RPMC. To clarify the mechanism of these inhibiting reactions, we investigated the effects of SRAE on cyclic AMP (cAMP). The level of cAMP in human leukemia cell line, HMC-1, when SRAE (1 mg/ml) was added, significantly increased compared with that of basal cells. These results indicate that SRAE may be beneficial in the treatment of immediate-type allergic reaction.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.169-174
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• 2012

Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

• 대한한의학회지
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• 제18권1호
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• pp.157-168
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• 1997

The purpose of this study is the separation of biologically active ingredients from Coptidis Rhizoma which has been widely used as one of oriental herbal medicine for body fever. In this study, berberine-type alkaloids were tested on their biological activities in the aspect of antibacterial, antitumor, anti-herpetic and anti-HIV activity. Contents of five major alkaloids for the various origin of Coptidis Rhizoma were assayed by HPLC. As the results, the content of berberine from Coptis chinensis and Coptis japonica were 6.78% and 7.09%, respectively. The contents of coptisine, jatrorrhizine, berberastine from Coptis chinensis were higher than those of Coptis japonica. The amount of palmatine from both species were almost the same. Surprisingly for antitumor experiment, all compounds have been shown remarkable activity, especially against SNU-l(human stomach cancer) cell line. Among the compounds purified through column chromatography, palmatine, coptisine, and jatrorrhizine inhibited the growth of K-562(human chronic myelogenous leukemia) cell line whereas jatrorrhizine has been shown the effective inhibition of A-549 (human lung) cell line at the same time.

• 한국동물학회지
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• 제35권2호
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• pp.219-225
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• 1992

The genetic variation in mitochondrial DNA (mtDNA) was analysed within and between two species of tree frogs. Hyla japonica and H. suweonensis from South Korea. Purified mtDNAs were digested with each of 11 restriction enLvmes which cleave at six base recognition sequences. The genome size of H. iaponica revealed ho types (20.0 $\pm$ 0.3 and 19.6 $\pm$ 0.3 kb) and this difference is explained by either addition or deletion of about 0.4 kb fragment. On the other hand, the genome sire of H. suueonensis was about 19.0 $\pm$ 0.4 kb only. For the analysis, level of fragment homology (F) and nucleotide sequence divergence (p) were estimated from comparisons of digestion profiles. Among four populations of H. iaponica, substantial mean sequence divergence was 0.017 (range 0.001-0.026); between identical types, 0.001 IslilaRl type) and 0.004 (Large type) respectively; between different ones, 0.024 (range 0.023-0.026). The level of sequence divergence between he species was 0.142 (range 0.131-0.146). This result suggested that he species ㅂwere distinctly differentiated species. The divergence time between ko species was estimated 7.1 million years.

• 한국작물학회지
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• 제45권5호
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• pp.288-293
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• 2000

Two rice cultivars (Oryza sativa L.), Hwa-seongbyeo of Japonica type and Taebackbyeo of Indica/Japonica type, were cultivated with hydroponic culture to examine nitrogen effects on the growth responses, contents and utilizations of carbohydrates, and the ripening velocity of grains with three different N levels. Plant height and tiller number were clearly increased to 80 ppm N level compared to 40 ppm N level and then they were slightly decreased in N level of 120 ppm. Higher dry weights were appeared with 80 ppm N level than did with other N levels, showing statistically differences in both cultivars and N levels, while dry weight of roots was heavier with decreasing the N levels. Therefore, T/R ratios were not significantly different among N levels, although there was statistically differences between rice cultivars. After the flowering stage, higher water-soluble carbohydrate (WSC) and water-insoluble carbohydrate (WISC) were contained in stem compared with other parts, showing that WISC of sheath and stem, unlike WSC, was significantly different among N levels. Starch of grain, WISC, was remarkedly increased from 3.0% at just after the flowering to 52.0% and 75.0% at 15 and 30 day after the flowering, respectively, showing that lower N application had faster accumulation of starch in rice grains. N would affect the contents of carbohydrates of each tissue, and starch accumulation in rice grains.

• 한국작물학회지
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• 제27권4호
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• pp.371-384
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• 1982

1. 내냉성품종의 요망은 고위도지대뿐만 아니라 열대지방에서도 간절하며 요망되는 내냉성품종의 특성도 여러 가지이다. 세계적으로 요망되는 내냉성을 IRRI에서는 편의상 a) 북해도형, b) 수원형, c) 태북일기작형, d) 태북이기작형, e) 열대고냉지형 및 f) Bangladesh형으로 구분하고 있다. 우리나라에서 요망되는 내냉성은 통일계품종에서 더욱 간절하며 생육전반에 걸쳐 Japonica 정도의 생리적 활성이 요망되지만 적어도 유묘기와 성숙기의 저온장해가 Japonica보다 크지 않은 것이 우선 당면한 문제라 하겠다. 일반계품종에서는 조생이면서 안정된 기본영양생장성을 가지고 고온에서 과민하지 않고 저온에서 생육지연이 비교적 작은 것이라면 좋을 것이다. 2. 국내에서의 내냉성계통검정방법과 시설은 통일계품종의 출현 이후 급속히 발전되었으며 저온항온기, 냉수 Tank, growth cabinet, phytotron, 춘천의 냉수포장, 진부, 운봉, 영덕의 내냉계통육종포 등 내냉계통선발을 위해서는 비교적 잘 갖춰진 편이다. 국외의 시설로는 IRRI의 냉수 Tank와 세대촉진시설 그리고 Banaue, Kathmandu나 Kashirnir의 자연생태적조건을 우리가 이용할 수 있을 것이다. 참고로 일본에서 보고된 생육각단계에서의 여러 가지 내냉성검정방법을 소개하였다. 3. 내냉성품종들이 가지고 있는 내냉형질의 단리는 아직 분명히 되어 있지 못하지만 a) 저온발아성, b) 저온유묘생장성, c) 저온묘변색 d) 저온발근력, e) 저온분벽력, f) 저온양분흡수능력, g) 저온동화력, i) 저온임실장해, j) 저온등숙장해 등에 품종간차가 분명한 것이 보고되어 있다. 위의 형질간의 상관관계는 형질에 따라 재료에 따라 구구하였다. 내냉성형질들의 유전에 관한 보고는 많지 않아 저온발아력에 관해 4개 이상의 다인자, 유묘저온생존력에 관해 단인자 또는 다인자, 유수형성기 저온저항력에 관해 4개 또는 그 이상의 다인자, 냉수포장에서의 저온저항력에 관해, 4개 이상의 유전자가 관여되는 것으로 그리고 heritability는 항상 높은 것으로 보고되어 있지만 같은 그 재료들을 가지고 다른 저온에서 검토하면 상이한 결과가 나올 것은 쉽게 짐작할 수 있다. 여러 가지 내냉성검정기술의 발전과 IRRI의 국제 내냉성품종연락시험으로 Japonica, Indica, Bulu 등에서 다같이 많은 품종들이 내냉성모본으로 검정되어 있다. 4. 육종방안을 구상할 때 고려해야 할 사항들 a) 유전질의 다양화, b) 내병충성의 집적, c) 지역별 적응품종의 동정, d) 장려품종의 체계적 통제 등을 고려하면서 단기적 및 장기적 육종방안을 제안하였다. 단기적으로는 일반계품종을 대상으로 하되 우리나라 기존품종을 기초로 알려진 내냉성품종을 가지고 생육일수와 내냉성을 조정하는 한편 극조생내냉성 찰벼모본에 내충내병인 Indica 모본을 일회친으로 Japonica로 Back cross하면서 내병충성을 유지한 계통을 선발하여 이것으로 내냉조합에 내병충성을 첨가시키도록 한다. 장기적 방안으로는 Japonica뿐만 아니라 Indica, Javanica 등 모든 내냉성모본을 이용하여 germplasm의 다양화를 꾀하며 모본별 형질별 내냉성유전자를 동정하여 이들을 종합하여 생육전기간을 안정하게 경과할 수 있게 내냉성을 향상하고 내병충성을 첨가하는 단계적인 과정을 제안하였다. 육종효율을 높이기 위하여 세대촉진, 화분배양과 국제협력의 필요성을 강조하였다.

• Applied Biological Chemistry
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• 제22권3호
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• pp.181-189
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• 1979

본(本) 연구(硏究)는 수도(水稻)의 파종(播種) 및 이앙기(移秧期)를 달리하고 시비량(施肥量)에 차이(差異)있는 조건(條件)에서 Japonica계(系) 품종(品種)과 통일계품종(統一系品種)을 대상(對象)으로 생육시기별(生育時期別) 수도체(水稻體)의 형성(形成)과 영양상태(營養狀態)를 주요성분(主要成分)의 화학분석(化學分析)을 통(通)하여 비교(比較) 검토(檢討)하고 소요(所要) 삼요소량(三要素量)을 추정(推定)하며 이들과 수도(水稻)의 수량(收量)및 수량구성요소(收量構成要素)와의 관계(關係)를 해명(解明)하여 안정(安定)된 증수(增收)를 위한 시비기술(施肥技術)의 기초적자료(基礎的資料)를 확립(確立)하고저 설계(設計)되었으며 그의 일부(一部)로서 수량(收量) 및 수량구성요소(收量構成要素)에 미치는 영향에 대하여 얻은 결과(結果)를 보고(報告)한다. 1. 간장(稈長)은 질소수준(窒素水準)이 높아짐에 따라 커지는 경향(傾向)이었으며 통일계품종(統一系品種)에서는 보통시비구(普通施肥區)와 다량시비구간(多量施肥區間)에 큰 차이(差異)가 없었다. 2. 수장(穗長)은 무비구(無肥區)에 비(比)하여 보비(普肥)및 다비구(多肥區)가 높았으나 그 경향(傾向)은 현저(顯著)하지는 않았다. 3. 질소수준(窒素水準)이 높아짐에 따라 수수(穗數)도 증가(增加)하였으며 Japonica형(型) 품종(品種)이 통일계(統一系)의 것보다 큰 반응(反應)을 보였다. 4, 수당입수(穗當粒數)는 통일계품종(統一系品種)에서는 무비(無肥)에 비(比)하여 보비(普肥)및 다비구(多肥區)에서 현저(顯著)히 높았으며 Japonica형(型) 품종(品種)에서는 그 변이폭(變異幅)이 좁았다. 5. 1,000입중(粒重)은 적기이앙(適期移秧)에서는 시비수준간(施肥水準間)에 유의성(有意性)이 없었으나 만기이앙(晩期移秧)의 경우에는 점차(漸次) 감소(減少)하는 경향(傾向)이 있다. 6. 등숙율(登熟率)은 적기이앙(適期移秧)에서는 질소수준간(窒素水準間), 품종간(品種間)의 어느 경우에도 유의성(有意性)이 없었으나 만기이앙(晩期移秧)에서는 질소수준간(窒素水準間) 및 품종간(品種間)에 고도(高度)의 유의성(有意性)이 인정(認定)되었다. 7. 수량(收量)에 대하여는 질소(窒素)의 수준간(水準間), 품종간(品種間)에 고도(高度)의 유의성(有意性)을 보였으며 통일계품종(統一系品種)에서는 다비구(多肥區)보다 보비구(普肥區)에서 더 높은 수량(收量)을 보였다. 8. 고중(藁重)은 질소수준(窒素水準)이 높아짐에 따라 커지는 경향(傾向)을 보여 통일계품종(統一系品種)에서는 보비구(普肥區)와 다비구간(多肥區間)에 큰 차이(差異)가 없었다.