• Korean Journal of Microbiology
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• v.21 no.4
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• pp.213-220
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• 1983

Conditions for isolation of protoplasts from conidiospores of Trichoderma koningii ATCC 26113 were tested. Maximum production of conidial protoplasts was obtained by preincubation of conidiospores on liquid minimal medium for 8 1/2 hrs. and by reaction with cell wall lytic enzyme for 3 hrs. Among effective cell wall lytic enzymes (Driselase, p-Glucuronidase, Novozyme and Zymolyase), Driselase was the most effective one on the production of conidial protoplasts. The production of conidial protoplasts was also enhanced by addition of 2-Deoxy-D-Glucose $(25{\mu}g/ml)$ into liquid minimal medium. Over 70% of the initial swollen conidia, preincubated in liquid minimal medium supplemented with 2-Deoxy-D-Glucose $(25{\mu}g/ml)$, were converted to protoplasts by incubation with 2% (w/v) commercial lytic enzyme Driselase at $28^{\circ}C$ for 3 hrs. The reversion frequency of the conidial protoplasts was about 30 times (25-50%) higher than that of mycelial protoplasts (0.6-1.3%).

• The Journal of Korean Institute of Communications and Information Sciences
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• v.15 no.3
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• pp.252-259
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• 1990

This paper present new scheme for a Side Wall Masked Isolation(SWAMI) technology which take all the advatages provided by conventional LOCOS process. A new SWAMI process incorporates a sloped sidewall by reactive ion etch and a layer of thin nitride around the side walls such that both intrinsic nitride stress and volume expansion induced stress are greatly reduced. As a fabricate results, a defect-free fully recessed zero bird's beak local oxidation process can be realized by the sloped wall anisotropic oxide isolation. No additional masking step is required. The leakage current of PN diodes of this process were reduced than PN diode of conventional LOCOS process. On the other hand, the edge junction part was larger than the flat juction part in the density of leakage current.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.10a
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• pp.167-175
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• 2000

Seismic analyses of structures can`t be performed without considering the effect of soil-structure interaction and seismic responses of a structure taking into account the stiffnesses of a foundation-soil system show a significant difference from those with a rigid base. However, current seismic analyses of apartment building structures were carried out assuming a rigid base and ignoring the characteristics of a foundation and the properties of the underlying soil. In this study, seismic analyses of apartment buildings of a particular wall-slab structural type were carried out comparing seismic response spectra of a flexible base with those of a rigid base and UBC-97. Wall-slab type low-rise or mid-height apartment buildings built on the deep soil layer showed a rigid body motion with the reduced seismic responses due to the base isolation effect, indicating that it is too safe but uneconomical to utilize the design spectra of UBC-97 for the seismic analysis of a wall-slab type apartment buildings due to the too conservative design.

• Journal of the Korean Geosynthetics Society
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• v.5 no.2
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• pp.23-30
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• 2006

This paper aims at investigating the material and dimensions of a wall barrier such as installation depth, thickness, and distance from vibration source by means of finite element method (FEM). In this study, various materials such as concrete, EPS, and steel were adopted. The results showed that the efficiency of a wall barrier appears to be more dependent on installation depth than thickness. In addition, a concrete wall barrier is less efficient than any other wall barrier. In contrast, EPS wall barriers seem to be much sensitive to the wall thickness than the depth.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2009.04a
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• pp.524-525
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• 2009

Heavy-weight impact noise(HN) is the most irritating noise in Korean apartment houses. It has been proclaimed standard floor system of 210mm thick slab with isolation material in the wall type structure. But this regulation is applied only new construction field and is not considered remodeling field. In general, the LN can be reduced by using isolation material but HN is known as relating with stiffness, strength & boundary condition of slab. Therefore it is difficulty in improving the HN on remodeling field. In this study, We conducted the reinforcement of concrete slab using C.F.S.(carbon fiber sheet), steel plate and steel beam after on-dol with isolation material on the remodeling field. As the test results, It appeared using C.F.S was no improved but using steel plate & steel beam were a little improved on HN.

• Structural Engineering and Mechanics
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• v.52 no.5
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• pp.875-887
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• 2014

The aim of the shaking table experiment is to verify the isolation effect of a storage liquid tank with multiple friction pendulum bearings. A 1:20 scale model of a real storage liquid tank that is widely used in the petroleum industry was examined by the shaking table test to compare its anchored base and isolated base. The seismic response of the tank was assessed by employing the time history input. The base acceleration, wave height and tank wall stress were used to evaluate the isolation effect. Finally, the influences of the bearing performance that characterizes the isolated tank, such as the friction force and residual displacement, were discussed.

• International Journal of High-Rise Buildings
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• v.4 no.3
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• pp.181-189
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• 2015

Shimizu Corporation Tokyo Headquarters, one of the city's leading office buildings, features many pioneering technologies that contribute to a sustainable society through environmental stewardship and a sophisticated disaster management facility. In terms of structural engineering, a seismic isolation system incorporating reinforced concrete core walls and precast concrete perimeter frames create a robust structure in the event of a large earthquake. In addition to the seismic resistance of the structure, several pioneering construction methods and materials are adopted. This office building can serve as a basis for new design and construction approaches and methodologies to ensure safe and economical structures.

• Earthquakes and Structures
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• v.4 no.6
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• pp.649-670
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• 2013

It is known that a base-isolated building exhibits a large response to a long-duration, long-period wave and an inter-connected system without base-isolation shows a large response to a pulse-type wave. To compensate for each deficiency, a new hybrid passive control system is investigated in which a base-isolated building is connected to another building (free wall) with oil dampers. It is demonstrated that the present hybrid passive control system is effective both for pulse-type ground motions and long-duration and long-period ground motions and has high redundancy and robustness for a broad range of disturbances.

• Structural Engineering and Mechanics
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• v.23 no.6
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• pp.615-634
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• 2006

Seismic response of the liquid storage tanks isolated by the elastomeric bearings and sliding systems is investigated under near-fault earthquake motions. The fault normal and parallel components of near-fault motion are applied in two horizontal directions of the tank. The continuous liquid mass of the tank is modeled as lumped masses known as sloshing mass, impulsive mass and rigid mass. The corresponding stiffness associated with these lumped masses has been worked out depending upon the properties of the tank wall and liquid mass. It is observed that the resultant response of the isolated tank is mainly governed by fault normal component with minor contribution from the fault parallel component. Further, a parametric study is also carried out to study the effects of important system parameters on the effectiveness of seismic isolation for liquid storage tanks. The various important parameters considered are: aspect ratio of tank, the period of isolation and the damping of isolation bearings. There exists an optimum value of isolation damping for which the base shear in the tank attains the minimum value under near-fault motion. The increase of damping beyond the optimum value will reduce the bearing and sloshing displacements but increases the base shear. A comparative performance of five isolation systems for liquid storage tanks is also studied under normal component of near-fault motion and found that the EDF type isolation system may be a better choice for design of isolated tank in near-fault locations. Finally, it is also observed that the satisfactory response can be obtained by analysing the base-isolated tanks under simple cycloidal pulse instead of complete acceleration history.

• Korean Journal of Microbiology
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• v.24 no.3
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• pp.295-301
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• 1986

The bacteria, which were capable of producing ${\beta}-1$, 3-glucanase inducibly by utilizing cell wall of Aspergillus fumigatus as a sole carbon source, were isolated from soil in the campus of Kyungpook National University. Among them, the strain which produced the enzyme excellently was selected and identified to be Pseudomonas stutzeri KF 13 by morphological, cultural and physiological examination. The optimal conditions for the enzyme production from Pseudomonas stutzeri KF 13 were investigated. the enzyme production was reached maximum state shen the broth cultured for 72hr at $30^{\circ}C$. And the enzyme showed the highest activity in the medium containing 3.5% cell wall as an inducer, 15% yeast autolysate as a nitrogen source and 0.05% $MnSO_4$ at pH 7.5.