• 콘크리트학회논문집
• /
• 제20권5호
• /
• pp.643-651
• /
• 2008

본 연구의 목적은 면진장치 설계 절차 체계화에 따른 면진건물의 응답특성을 사용지진 기록에 따라 평가하는데 있다. 이를 위한 대상 건물은 면진에 적합한 기초 면진 적용한 20층 주거 건물로, 지진기록 사용에 따른 응답특성을 평가하였다. 그 결과 면진건물의 시간이력해석을 위한 지진파의 선정은 응답 결과에 많은 차이가 발생할 수 있으므로 관련기준의 제정이 필요한 것으로 판단된다. 본 연구에서 제안한 지진기록을 이용한 면진구조물의 시간이력해석 결과, 면진층 변위, 층간변위비, 면진장치의 거동이 적절한 것으로 평가되었다. 결론적으로 본 연구에서 제안한 지진기록 및 면진장치 선정 결과, 설계된 면진건물의 지진저항 성능이 우수함을 확인할 수 있었다.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• 제6권11호
• /
• pp.2764-2783
• /
• 2012

Network virtualization supports future Internet environments and cloud computing. Virtualization can mitigate many hardware restrictions and provide variable network topologies to support variable cloud services. Owing to several advantages such as low cost, high flexibility, and better manageability, virtualization has been widely adopted for use in network virtualization platforms. Among the many issues related to cloud computing, to achieve a suitable cloud service quality we specifically focus on network and performance isolation schemes, which ensure the integrity and QoS of each virtual cloud network. In this study, we suggest a virtual network platform that uses Xen-based virtualization, and implement multiple virtualized networks to provide variable cloud services on a physical network. In addition, we describe the isolation of virtual networks by assigning a different virtualized network ID (VLAN ID) to each network to ensure the integrity of the service contents. We also provide a method for efficiently isolating the performance of each virtual network in terms of network bandwidth. Our performance isolation method supports multiple virtual networks with different levels of service quality.

• 한국전자파학회논문지
• /
• 제19권8호
• /
• pp.820-825
• /
• 2008

본 논문에서는 $2.3{\sim}2.4$ GHz 대역 무선 휴대 단말기용 내장형 MIMO 안테나 시스템의 격리도를 향상시키는 방법을 제시하였다. 제안된 MIMO 안테나 시스템은 2개의 안테나 사이의 그라운드 기판에 다수 개의 L-slot으로 구성된 multi-slots를 위치시키며, multi-slots가 대역 차단 필터처럼 작용하여 안테나 간의 상호 결합을 억제시켜 격리도를 향상시킨다. 6쌍의 L-slots를 채택한 MIMO 안테나 시스템은 -26.4 dB의 반사 손실과 -37.5 dB의 격리도를 나타내었다.

• 한국미생물·생명공학회지
• /
• 제24권2호
• /
• pp.178-184
• /
• 1996

Dryfilm method by using 3M Petrifilm$^{TM}$ has been examined to replace conventional agar method for isolation of microorganisms from foods. The objectives of the present study were to evaluate suitability of dryfilm method as a microbial isolation method and to determine the effect of antimicrobial agent on dryfilm for isolation of microorganisms from foods. Five different foods, milk, ground beef, fishery surimi, Takju and wheat flour were used to isolate the natural microflora in foods and the inoculated Escheri chia coli. Standard method agar (SMA, Difco) and Petrifilm$^{TM}$ aerobic count (PAC, 3M) were used to isolate total microorganisms from foods. Violet red bile agar (VRBA), brilliant green lactose bile (BGLB) broth and Petrifilm$^{TM}$ coliform count (PCC, 3M) were used to isolate coliforms from foods. E. coli broth (EC broth) and Petrifilm$^{TM}$ E. coli count (PEC, 3M) were used to isolate E. coli from foods. Acidified potato dextrose agar (APDA) and Petrifilm$^{TM}$ yeast & mold count (PYMC, 3M) were used to isolate yeasts and molds from foods. Total aerobic plate counts isolated from five different foods by SMA and PAC (3M) were riot significantly different each other at P<0.05 level and were highly correlated each other ($\geq$0.96). Mugwort extract as an antimicrobial agent did not affect microbial enumeratiion of Dryfilm. Significantly higher number of coliform colonies were formed on VRBA than PCC (3M) from ground beef, but they were not significantly different in coliform colonies from milk samples. PCC (3M) and BGLB were not significantly different for enumeration of coliforms in milk and beef samples. Significantly higher number of E. coli were isolated by EC broth than PEC from ground beef, but these were not significontly different for enumeration of E. coli from milk. Yeast and mold counts isolated from Takju and wheat flour by APDA and PYMC (3M) were not significantly different at P<0.05 level. These data indicate that dryfilm method by using 3M Petrifilm$^{TM}$ can be successively used as an alternative to conventional agar method for enumeration of microorganisms in various foods.

• Journal of Power Electronics
• /
• 제17권1호
• /
• pp.305-313
• /
• 2017

A novel interference isolation method is proposed by using several designed coils in capacitive power transfer systems as isolation impedances. For each designed coil, its stray parameters such as the inter-turn capacitance, coil resistance and capacitance between the coil and the core, etc. are taken into account. An equivalent circuit model of the designed coil is established. According to this equivalent circuit, the impedance characteristic of the coil is calculated. In addition, the maximum impedance point and the corresponding excitation frequency of the coil are obtained. Based on this analysis, six designed coils are adopted to isolate the interference from power delivery. The proposed method is verified through experiments with a power carrier frequency of 1MHz and a data carrier frequency of 8.7MHz. The power and data are transferred parrallelly with a data carrier attenuation lower than -5dB and a power attenuation on the sensing resistor higher than -45dB.

• Journal of Microbiology and Biotechnology
• /
• 제11권5호
• /
• pp.890-894
• /
• 2001

The isolation of genomic DNA from mammalian cells is usually performed by cell lysis followed by protein digestion, extraction, and finally, ethanol precipitation of the chromosomal DNA. However, in the case of large sample numbers or when only small amounts of starting materials are available, such conventional methods are not efficient and are cumbersome to be applied. Some alternative methods have been described as well as having commercial DNA isolation kits to be available, nevertheless, there is room left for much improvement. In the present study, a novel method is introduced, where it simplifies conventional protocols by omitting some time-consuming steps such as protease incubation or DNA precipitation and its resuspension. Using paramagnetic silica resins, the genomic DNA was purified over a magnetic field, and the bound DNA was eluted with a low-salt buffer. The fidelity and effectiveness of this novel method was determined by using normal and apoptotic cells as a starting material and then compared to other protocols. The high speed and convenience along with its high efficiency in detecting apoptotic chromosomal DNA will prove this method to be an improved alternative in the isolation of genomic DNA from mammalian cells.

• 한국수정란이식학회지
• /
• 제33권4호
• /
• pp.327-336
• /
• 2018

To date, there are no protocols optimized to the effective separation of spermatogonial stem cells (SSCs) from testicular cells derived from mouse testes, thus hindering studies based on mouse SSCs. In this study, we aimed to determine the most efficient purification method for the isolation of SSCs from mouse testes among previously described techniques. Isolation of SSCs from testicular cells derived from mouse testes was conducted using four different techniques: differential plating (DP), magnetic-activated cell sorting (MACS) post-DP, MACS, and positive and negative selection double MACS. DP was performed for 1, 2, 4, 8, or 16 h, and MACS was performed using EpCAM ($MACS^{EpCAM}$), Thy1 ($MACS^{Thy1}$), or GFR ${\alpha}1$ ($MACS^{GFR{\alpha}1}$) antibodies. The purification efficiency of each method was analyzed by measuring the percentage of cells that stained positively for alkaline phosphatase. DP for 8 h, $MACS^{Thy1}$ post-DP for 8 h, $MACS^{GFR{\alpha}1}$, positive selection double $MACS^{GFR{\alpha}1/EpCAM}$, and negative selection double $MACS^{GFR{\alpha}1/{\alpha}-SMA}$ were identified as the optimal protocols for isolation of SSCs from mouse testicular cells. Comparison of the purification efficiencies of the optimized isolation protocols showed that, numerically, the highest purification efficiency was obtained using $MACS^{GFR{\alpha}1}$. Overall, our results indicate that $MACS^{GFR{\alpha}1}$ is an appropriate purification technique for the isolation of SSCs from mouse testicular cells.

• Journal of Ginseng Research
• /
• 제10권1호
• /
• pp.21-26
• /
• 1986

한국산 수삼으로부터 저압 액체크로마토그래피를 이용하여 conjugated poly-ynes(폴리아세틸렌) 정유성분을 분리 동정하였다. 이 방법은 종래의 방법에 비해 과정이 간편할 뿐만 아니라 폴리아세틸렌을 더욱 순수하게, 더욱 높은 수득률로, 그리고 더욱 빨리 분리할 수 있는 이점이 있다.

• 제어로봇시스템학회논문지
• /
• 제11권3호
• /
• pp.200-206
• /
• 2005

In this paper, a redundancy management system for aircraft is studied, and FDI (Fault Detection and Isolation) algorithm of inertial sensor system is proposed. UAV system cannot allow triple or quadruple hardware redundancy due to the limitations on space and weight. In the UAV system with dual sensors, it is very difficult to identify the faulty sensor. Also, conventional FDI method cannot isolate multiple faults in a triple redundancy system. In this paper, hardware based FDI technique is proposed, which combines a parity equation approach with the wavelet based technique, which is a model-free FDI method. To verify the effectiveness of the proposed FDI method, numerical simulations are performed.

• 한국지진공학회:학술대회논문집
• /
• 한국지진공학회 2001년도 추계 학술발표회 논문집 Proceedings of EESK Conference-Fall 2001
• /
• pp.348-355
• /
• 2001

A computer program named \"NLDA-BIS\", which runs under the MATLAB environment, is developed fur seismic response analysis of base isolated structures. This program can explicitly model the various nonlinear isolation elements such as elastomeric bearings, sliding bearings and general viscous dampers, and so on. Newmark'\`s constant average acceleration method fur calculating the responses in time domain and the iterative pseudo-force method for treating the nonlinear isolation forces are adopted. For capturing the hysteretic behavior of isolation elements, the modified Wen's equations are adopted and solved by the numerical differentiation formula method. To verify the validity of the developed program, the seismic responses of a six-story reinforced concrete base isolated structure are calculated and compared with results obtained by the program \"3D-BASIS\" developed at the State University of New York at Buffalo which is the most widely used code far analyzing isolated structures today.ed structures today.