• Journal of the Korean Wood Science and Technology
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• 제50권3호
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• pp.159-166
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• 2022

CO₂ emissions are the primary reason for global warming; hence, biological and chemical technologies for converting CO₂ into useful compounds are being actively studied. Biological methods using enzymes can convert CO₂ under mild conditions. Formate dehydrogenase (FDH) is a representative CO₂ conversion enzyme. Its function was revealed after isolation from bacteria, yeast, and plants. In this study, we evaluated the CO₂ conversion potential of FDH isolated from wood-rotting fungi. After isolating the FDH gene (TvFDH) from Trametes versicolor, we cloned the full-length FDH from T. versicolor and expressed it in a cell-free expression system. The gene encoding TvFDH was identified as 1,200 bp open reading frame (ORF) and the expected molecular weight of the protein was approximately 42 kDa. Overexpression of the recombinant crude protein including TvFDH was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Enzyme activities and metabolite analyses confirmed the efficiency of TvFDH for CO₂ reduction.

• 인간식물환경학회지
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• 제21권6호
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• pp.565-574
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• 2018

Previous studies have shown that forest therapy program can help prevent dementia. However, few studies have focused on low-income elderly people living alone. The current study examined the meanings that the elderly living alone receiving medical care assigned to the urban forest therapy program, as a way to understand the pathways that nature-based intervention affect preventing dementia. Twenty-one participants were recruited and they participated in a five-week urban forest therapy program. Semi-structured interviews were carried out with 21 participants who experienced the urban forest therapy program, and analyzed qualitative data using thematic analysis. Results showed that all themes identified were related to connectedness with oneself, neighbors and nature. Awarenesses of change were consisted of positive and negative themes. The themes of positive awareness were improvements of mental and emotional condition, feelings of isolation and loneliness, and health-related lifestyle. The negative themes were terminations of short-term programs and inconvenient access to the urban forest. Based on these data, we suggest an urban green welfare framework for future research and interventions for preventing dementia of underprivileged elderly group.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2005년도 춘계학술대회 및 국제심포지움 초록집
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• pp.115-115
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• 2005

• 한국산림과학회지
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• 제71권1호
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• pp.66-73
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• 1985

본(本) 연구(硏究)는 국내(國內) 삼림생태계(森林生態係)의 우점수종(優占樹種)으로서 생태적(生態的) 및 경제적(經濟的)으로 유망(有望)한 참나무류(類)의 육종(育種) 및 우량(優良)크론의 대량증식(大量增殖)을 저해(低害)하고 있는 인공교잡(人工交雜)과 무성번식(無性繁殖)의 난점(難點)을 타개하기 위한 세포배양(細胞培養) 및 체세포(體細胞) 교잡(交雜)에 필요(必要)한 기초자료(基礎資料)를 획득하기 위하여 실시(實施)되었다. 주요시험결과(主要試驗結果)를 요약(要約)하면 다음과 같다. 1) 미경화엽(未硬化葉)과 왕성(旺盛)한 성장(成長)을 보이는 Callus가 지속적인 원형질체(原形質體) 공급원(供給源)으로 적합(適合)하며 2) 원형질체(原形質體) 라출(裸出)을 위(爲)한 효소용액(酵素溶液)의 조성(組成)은 Cellulase Onozuka R-10($20g/{\ell}$), Macerozyme R-10($10g/{\ell}$), Pectinase($250units/{\ell}$), $CaCl_2$, $2H_2O$(14mM), $MgSO_4{\cdot}7H_2O$(1.8mM), $KNO_3$(1.0mM), $H_3BO_3$(1.0mM), $KH_2PO_4$(0.2mM), KI($1.0{\mu}M$), 1.4-dithiothreitol(0.1mM), mannitol(0.6M)을 조합(組合)한 것의 성적(成績)이 가장 좋았다. 3) 세포융합율(細胞融合率)이 가장 높았던 원형질체(原形質體)의 밀도(密度)는 $2{\times}10^5/ml$이었다. 이는 본(本) 시험(試驗)에 채용(採用)된 원형질체밀도중(原形質體密度中) 가장 높은 밀도(密度)였다. 4) 세포융합유도(細胞融合誘導)를 위(爲)한 polyethylene glycol 처리(處理)의 적정농도(適正濃度)와 처리시간(處理時間)은 각각(各各) 29%(w/v)와 5~10분(分)으로 나타났다.

• Journal of Forest and Environmental Science
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• 제27권3호
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• pp.183-194
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• 2011

This study reviewed on the research trend of sources and utilization of the byproducts(Lignin) from bioethanol production process with lignocellulosic biomass such as wood, agri-processing by-products(corn fiber, sugarcane bagasse etc.) and energy crops(switch grass, poplar, Miscanthus etc.). During biochemical conversion process, only Cellulose and hemicellulosic fractions are converted into fermentable sugar, but lignin which represents the third largest fraction of lignocellulosic biomass is not convertible into fermentable sugars. It is therefore extremely important to recover and convert biomass-derived Lignin into high-value products to maintain economic competitiveness of cellulosic ethanol processes. It was introduced that lignin types and characteristics were different from various isolation methods and biomass sources. Also utilization and potentiality for market of those were discussed.

• 환경영향평가
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• 제22권2호
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• pp.147-156
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• 2013

Myanmar(Burma) has been preserved valuable environmental resources because of its political isolation. But recently, Myanmar has moved a capital city(Naypyidaw) at central forest area and it has been urbanized radically since 2005. In this paper, we built multi-temporal land cover map from Landsat images of 1970s to 2012 with ENVI 4.5 software. For a broad approach, administrative district Yamethin which includes Naypyidaw is classified into 3 classes and with only Naypyidaw region is classified with 4-5 classes to analyse specific changes. And with forest cover extracted by Object Oriented Classification, we evaluated forest fragmentation before and after the development using Patch Analyst(FRAGSTATs 3.3) at Yamethin area. For Yamethin area, there were significant forest cover change, 51% in 1999 to 48% in 2012, and for Naypyidaw area, 67% in 1999 to 57% in 2012 respectively. Also landscape indices resulted from Patch Analyst concluded that the total edge, edge density and mean shaped index of forest patches increased and total core area is decreased. It is attributed from land cover change with urbanization and agricultural land expansion.

• 한국환경생태학회지
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• 제12권4호
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• pp.317-330
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• 1999

한려해상국립공원의 통영지구와 욕지도에서 현존식생과 식생구조를 파악하기 위하여 1998년 7월에 37개의 조사구를 설치하여 조사하였다. 조사대상지의 식생은 지리적 격리와 인위적 교란에 의해 지역간 식생구조가 상이하였다. 대상지의 식생은 곰솔림, 곰솔 - 소나무와 낙엽활엽수가 경쟁하는 혼효림, 낙엽활엽수림, 곰솔 - 소나무와 상록활엽수가 경쟁하는 혼효림, 상록활엽수림으로 총 5개 그룹으로 대별되었다. 조사대상지 대부분의 현존 식생은 곰솔림으로 이루어졌으며, 상록활엽수림은 추봉도, 용초도, 비진도, 욕지도, 유재도에 작은 면적으로 분포하였다. 통영지구와 욕지도의 식생구조는 소나무, 곰솔, 낙엽활엽수에서 구실잣밤나무 등을 거쳐 참식나무, 후박나무, 생달나무로 식생천이가 예상된다.

• 한국환경복원기술학회지
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• 제13권5호
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• pp.135-145
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• 2010

This study was carried out to investigate the current urban forest network and future plan of forest framework using nearest features program where stepping stones within 5km from the core forest were selected in the program. We found several conclusions as follows: First, we found that cities in inland area including Daegu, Dajeon and Gwangju have quite different types of forest network with comparing to the cities nearby coastline including Busan, Incheon and Ulsan. The cities in inland area have large mountain patch around each city. However they have small and lower number of island forests within their urban area. Otherwise, cities nearby coastline have more forest patches than in the cities in inland area, and Busan and Incheon especially have strong forest network using various size of forest patches. Second, Daegu and Daejeon have much smaller forest patches distributed in each urban area. So additional forest patches should be added to have highly strong forest network within urban area. Third, Ulsan and Gwangju have most stepping-stone forests close to the large mountain patches in suburban area, which are not able to connect to the forest patches in the central area of each city So additional forest patches are needed to be added in the central area of each city for reinforcing the effect of stepping stone in the central area. Though there should be an addition approach except for forest size and its isolation to construct the ecological network in an urban area, this indices can be a good method to check an environmental and ecological status in an urban area.

• 한국산림과학회지
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• 제97권2호
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• pp.187-195
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• 2008

Picea jezoensis (Sieb. et Zucc.) Carriere is one of the major and widespread components of the cold-temperate and boreal forests in Russian Far East, northeast China, Korea, and Japan. However, it is restricted to a highly fragmented range in South Korea with small populations. Mean expected heterozygosity $(H_e)$ based on 22 loci in 11 isozyme systems was 0.077 for four sampled populations that covered the whole distribution range of P. jezoensis in South Korea. This value is within the range reported for conifers, but it is very low compared to that of other spruce species as well as that of P. jezoensis populations in Russian Far East. Most populations had a slight excess of heterozygotes and the Wright's $F_{IS}$ (-0.019) was comparable to that previously reported for other spruce species. In all of the four populations, the Wilcoxon sign-rank test indicated no greater heterozygosity than that expected for populations at mutation-drift equilibrium, suggesting that the populations have not been bottlenecked recently. Despite a fragmented range and isolated populations, population differentiation was not high $(F_{ST}=0.047)$ and the number of migrants per generation was 5.09. Nei's genetic distances were also small $({\bar{D}}=0.005)$ but strongly related to geographic distances between populations, suggesting an Isolation by Distance. The northernmost isolate, Mt. Gyebang population was genetically distinct from the other three populations. Implications for the conservation of genetic variation of P. jezoensis in South Korea were discussed.

• 한국산림과학회지
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• 제98권5호
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• pp.531-538
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• 2009

We used enzyme electrophoresis to evaluate genetic diversity in five populations of endemic ash, Fraxinus chiisanensis in Korea. Of 15 putative allozyme loci examined 26.7% were polymorphic and expected heterozygosity for the species was low (0.082). Within the range, population were highly differentiated ($F_{ST}$=0.356) and little genetic variation was explained by geography. The pattern of distribution of variation showed low genetic variation within populations and pronounced divergence among populations, which was consistent with the prediction for the effects of limited gene flow and local genetic erosion. Although the frequencies of male plants were dominant ranging from 79.3% to 89.4%, most mating events seems to be inevitable mating between relatives in small populations based on heterozygote deficiency of this species. Small effective population size and the limited dispersal contributed to the low rates of gene flow within as well as between populations.