• 제목/요약/키워드: isolation, identification

검색결과 1,062건 처리시간 0.049초

Sasa borealis의 Diastereomeric 성분들의 역상 고속액체크로마토그래프 분석방법 (Reverse-Phase HPLC Method for Identification of Diastereomeric Constituents from Sasa borealis)

  • 정연희;이준;권영주;서은경
    • 약학회지
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    • 제50권1호
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    • pp.21-25
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    • 2006
  • Reiterated normal-phase column chromatography lead to the isolation and purification of six known compounds but for the first time from the whole plant of Sasa borealis (Hack.) Makino (Gramineae): tricin 4'-O-(erythro-${\beta}$-guaia-cylglyceryl) ether (1), tricin 4'-O-(threo-${\beta}$-guaiacylglyceryl) ether (2), tricin 4'-O-[erythro-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (3), tricin 4'-O-[threo-${\beta}$-guaiacyl-(9'-O-acetyl)-glyceryl] ether (4), (-)-pinoresinol (5), and vanillin (6). The structures of the compounds (1-6) were established based on interpretation of high resolution NMR (COSY, HSQC, HMBC, and NOESY) spectral data. In particular, compounds 1 and 3 were diastereomers of compounds 2 and 4, respectively. These two sets of diastereomers were able to be simultaneously identified and quantified by a gradient reversed-phase HPLC method with UV photodiode array, This sensitive HPLC method is noteworthy as a simultaneous separation and identification method to test the extract of the family Gramineae which contains these compounds.

수동형 RFID 시스템 적용을 위한 SAW ID 태그 및 수신 시스템 구현 (SAW ID Tag and Receiver System for Passive RFID System Application)

  • 김재권;박주용;범진욱
    • 대한전자공학회논문지SD
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    • 제45권4호
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    • pp.64-71
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    • 2008
  • SAW(Surface Acoustic Waves)를 기반으로 passive RFID(Radio Frequency IDentification) tag 및 송수신 시스템을 제작하였다. Pulse position encoding 방식의 SAW ID 태그(tag)를 제작하여 amplitude on/off 방식의 SAW ID 태그에 비해 3배의 데이터 용량 증대 효과를 얻을 수 있었다. 또한, 수신 시스템을 heterodyne 방식과 direct conversion 방식으로 제작하였다. 측정 결과 direct conversion 방식으로 제작된 수신 시스템이 heterodyne 방식의 수신 시스템과 비교하여 isolation 특성이 10 dB 증가하였으며, 그 결과 무선 도달 거리가 증가했다.

Isolation and Morphological Identification of Apple Anthracnose Fungus of Colletotrichum sp. KV-21

  • Bajpai, Vivek K.;Choi, Seak-Won;Cho, Moon-Soo;Kang, Sun-Chul
    • 한국환경농학회지
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    • 제28권4호
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    • pp.442-446
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    • 2009
  • This study was undertaken to isolate and to identify a fungal pathogen Colletotrichum sp. KV-21 associated with apple anthracnose. Rotted Gala apples were used for the isolation of the fungus. The infected tissues were sterilized with 70% ethanol, washed with sterilized distilled water and were transferred to 50 ml containing potato broth (PDB) flasks. The peripheral hyphae of the fungal colony which developed from the infected tissues were isolated on to potato dextrose agar (PDA). On PDA plates the fungus grew well at $25^{\circ}C$ and occupied more than half of a 9 cm petri dish within 5 days. The fungal cultures on PDA were used for morphological observation and identification of the fungus. Conidiophores were produced on the gray to whitish sporodochial structures scattered on PDA plates which gave rise to conidiogenous cells. The structures of the conidia produced on PDA plates were subcylindrical to obovoid, fusoid, tapered and 4 to $6\;{\mu}m$ in size.

대전광역시와 충청남도 밭 토양으로부터 야생효모의 분리 및 동정 (Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea)

  • 한상민;한재원;배상민;박원종;이종수
    • 한국균학회지
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    • 제44권1호
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    • pp.1-7
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    • 2016
  • 우리나라 토양에서 효모 종 분포특성을 조사하기 위해 먼저 대전광역시를 포함하는 충청남도 전 지역의 밭 토양 61점을 2015년 6월부터 8월까지 수집하여 97균주 20종의 야생효모들을 분리하였고, polymerase chain reaction (PCR)을 통해 internal transcribed spacer (ITS) 부위와 26S rDNA의 D1/D2 부위의 염기서열 상동성 비교법을 이용하여 종을 동정하였다. 이들 가운데 Cryptococcus podzolicus가 11균주를 포함하는 Cryptococcus 속 균이 전체 분리 균주 중 16균주(16%)로 가장 많이 분리되었고, 다음으로 Debaryomyces hansenii와 Trichorsporon asahii도 각각 6주씩 분리되었다.