The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Isolation and Identification of Wild Yeasts from Soils of Fields in Daejeon Metropolitan City and Chungcheongnam-do, Korea

대전광역시와 충청남도 밭 토양으로부터 야생효모의 분리 및 동정

  • Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Han, Jae-Won (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Bae, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University) ;
  • Park, Won-Jong (Department of Food Technology, Kongju National University) ;
  • Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
  • 한상민 (배재대학교 바이오.의생명공학과) ;
  • 한재원 (배재대학교 바이오.의생명공학과) ;
  • 배상민 (배재대학교 바이오.의생명공학과) ;
  • 박원종 (공주대학교 식품공학과) ;
  • 이종수 (배재대학교 바이오.의생명공학과)
  • Received : 2015.12.05
  • Accepted : 2016.03.03
  • Published : 2016.03.31
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Abstract

This study focused on isolation and identification of wild yeasts from soils in fields near mountains and elucidation of its yeast distribution. Several kinds of yeasts were isolated from various soils of Daejeon metropolitan city and Chungcheongnam-do in Korea and identified by BLAST search of nucleotide sequences of internal transcribed spacer (ITS) region including 5.8S rRNA and D1/D2 region of 26S rDNA. Ninety-seven strains of 20 species from 61 soil samples were isolated, of which Cryptococcus podzolicus (11 strains), Debaryomyces hansenii (6 strains), and Trichosporon asahii (6 strains) were dominant species.

우리나라 토양에서 효모 종 분포특성을 조사하기 위해 먼저 대전광역시를 포함하는 충청남도 전 지역의 밭 토양 61점을 2015년 6월부터 8월까지 수집하여 97균주 20종의 야생효모들을 분리하였고, polymerase chain reaction (PCR)을 통해 internal transcribed spacer (ITS) 부위와 26S rDNA의 D1/D2 부위의 염기서열 상동성 비교법을 이용하여 종을 동정하였다. 이들 가운데 Cryptococcus podzolicus가 11균주를 포함하는 Cryptococcus 속 균이 전체 분리 균주 중 16균주(16%)로 가장 많이 분리되었고, 다음으로 Debaryomyces hansenii와 Trichorsporon asahii도 각각 6주씩 분리되었다.

Keywords

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