• 전기전자학회논문지
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• 제23권1호
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• pp.266-272
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• 2019

멀티콥터는 수직 이착륙 할 수 있다는 장점 때문에 활용이 증가하고 있으며 이러한 멀티콥터의 정상적인 동작을 위해서는 고장 검출과 분리 문제가 매우 중요하다. 본 논문에서는 해석적 방법에 기반하여 헥사콥터 구동기의 고장을 검출하고 분리하는 새로운 방법을 제안한다. 제안한 방법에서는 헥사콥터의 수학적 모델로부터 추정한 구동기의 각속도를 이용하여 잔차를 새롭게 정의하고 생성된 잔차와 문턱값을 비교하여 구동기의 고장을 검출한다. 고장이 검출되면 헥사콥터의 동역학 모델과 생성된 잔차를 결합하여 고장을 분리한다. 제안한 방법은 수학적 모델을 기반으로 하여 간단하면서도 효과적이다. 헥사콥터 구동기의 단일 고장 상황을 가정한 시뮬레이션을 통해 제안한 방법의 유효성을 검증하였다.

• Structural Engineering and Mechanics
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• 제85권6호
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• pp.825-835
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• 2023

The effective buckling length factor is an important parameter in the elastic buckling analysis of steel structures. The present article aims at developing a new method that allows the determination of the buckling factor values for frames. The novelty of the method is that it considers the interaction between the bracing and the elastic supports for asymmetrical frames in particular. The approach consists in isolating a critical column within the frame and evaluating the rotational and translational stiffness of its restraints to obtain the critical buckling load. This can be achieved by introducing, through a dimensionless parameter 𝜙_i, the effects of coupling between the axial loading and bending stiffness of the columns, on the classical stability functions. Subsequently, comparative, and parametric studies conducted on several frames are presented for assessing the influence of geometry, loading, bracing, and support conditions of the frame columns on the value of the effective buckling length factor K. The results show that the formulas recommended by different approaches can give rather inaccurate values of K, especially in the case of asymmetric frames. The expressions used refer solely to local stiffness distributions, and not to the overall behavior of the structure.

• Journal of Applied Biological Chemistry
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• 제57권3호
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• pp.259-265
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• 2014

Mitochondrial DNA (mtDNA)-depleted (${\rho}^0$) cells are often used as mtDNA recipients to study the interaction between the nucleus and mitochondria in mammalian cells. Therefore, it is crucial to obtain mtDNA-depleted cells with many different nuclear backgrounds for the study. Here, we demonstrate a rapid and reliable method to isolate mammalian mtDNA-depleted cells involving treatment with the antimitochondrial agents ethidium bromide (EtBr) and 2',3'-dideoxycytidine (ddC). After a short exposure to EtBr or ddC, followed by rapid clonal isolation, we were able to generate viable mtDNA-depleted cells from mouse and human cells and were able to successfully repopulate them with exogenous mitochondria from platelets isolated from mouse and human blood samples. These mtDNA-depleted cells can be used to characterize the nuclear mitochondrial interactions and to study mtDNA-associated defects in mammalian cells. Our method of isolating mtDNA-depleted cells is practical and applicable to a variety of cell types.

• 한국소음진동공학회논문집
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• 제25권3호
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• pp.191-198
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• 2015

Noise propagated through the ventilation openings of enclosures is actively controlled using an FIR filter. The enclosures considered in this paper are used for isolating noise due to machinery. This method is of limited use because of the ventilation openings through which most of noise is propagated. Feedforward control strategy is incorporated to minimize the acoustic power propagated through the openings. For the real-time implementation, although it is numerical study, the controller is implemented using an FIR filter. The acoustic transfer functions of the pressure on the openings of the enclosure to the primary source and to the secondary source are numerically calculated using the boundary element method. The performance analysis of the active control is conducted with the time-domain simulation using Matlab Simulink.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제6권11호
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• pp.2764-2783
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• 2012

Network virtualization supports future Internet environments and cloud computing. Virtualization can mitigate many hardware restrictions and provide variable network topologies to support variable cloud services. Owing to several advantages such as low cost, high flexibility, and better manageability, virtualization has been widely adopted for use in network virtualization platforms. Among the many issues related to cloud computing, to achieve a suitable cloud service quality we specifically focus on network and performance isolation schemes, which ensure the integrity and QoS of each virtual cloud network. In this study, we suggest a virtual network platform that uses Xen-based virtualization, and implement multiple virtualized networks to provide variable cloud services on a physical network. In addition, we describe the isolation of virtual networks by assigning a different virtualized network ID (VLAN ID) to each network to ensure the integrity of the service contents. We also provide a method for efficiently isolating the performance of each virtual network in terms of network bandwidth. Our performance isolation method supports multiple virtual networks with different levels of service quality.

• Asian-Australasian Journal of Animal Sciences
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• 제25권7호
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• pp.950-955
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• 2012

This study was conducted to determine how the isolation method of the porcine preantral follicles influenced the following follicular growth in vitro. Mechanical and enzymatical isolations were used for retrieving the follicles from prepubertal porcine ovaries, and in vitro-growth of the follicles and the expression of folliculogenesis-related genes were subsequently monitored. The enzymatic retrieval with collagenase treatment returned more follicles than the mechanical retrieval, while the percentage of morphologically normal follicles was higher with mechanical retrieval than with enzymatic retrieval. After 4 days of culture, mechanically retrieved, preantral follicles yielded more follicles with normal morphology than enzymatically retrieved follicles, which resulted in improved follicular growth. The mRNA expression of FSHR, LHR Cx43, DNMT1 and FGFR2 genes was significantly higher after culture of the follicles retrieved mechanically. These results suggest that mechanical isolation is a better method of isolating porcine preantral follicles that will develop into competent oocytes in in vitro culture.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.566-569
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• 2005

한국산 개비자나무로부터 새로운 개념의 homoharringtonine 분리 및 정제 공정을 개발하였다. 메탄올을 사용한 4회 반복 추출에 의해 biomass로부터 대부분(>99%)의 homoharringtonine을 회수 할 수 있었다. 흡착 공정에서는 활성 백토(active clay)를 사용하여 추출물에 포함되어 있는 식물유래 타르, 왁스 성분을 효과적으로 제거하였다. Silica gel low-pressure chromatography공정을 통해서 순도 52% 이상의 homoharringtonine을 얻었으며, HPLC 공정을 통해 고순도 및 고수율의 homharringtonine을 정제할 수 있었다. 정제된 homoharringtonine의 분자량 및 구조분석을 수행한 결과 표준물질(Homharringtonine)과 동일 물질임을 확인할 수 있었다.

• 지역사회간호학회지
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• 제19권1호
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• pp.101-111
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• 2008

Purpose: This study was to analyze the trends of research on school health by examining papers related to school health. Method: This studt was analyzed all related papers published in journals concerning school health from 2000 to 2006. The data were processed statistically by frequency percentage and content analysis. Results: Among 463 papers in 6 journals related to school health. 'The Journal of the Korean Society of School Health' had the largest number of articles. Descriptive research design was the most frequently used research design, and students were the most frequent research target. Among research writers, the professor group was largest, and schools were the most frequently used place for research. The most common sampling method was convenient sampling. The most common research instrument was questionnaire survey. Parametric statistics were the main methods of data analysis. Among the themes of school health, the adoption of nursing process frames and assessment domains occupied the largest portion. Health was the most common key concept, and the mainly used theory in the journals was factor-isolating. Conclusion: Through the analysis of research trends, we expect a higher level of school health research.

• Animal cells and systems
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• 제7권2호
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• Macromolecular Research
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• 제11권5호
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• pp.341-346
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• 2003

A poly(ethylene oxide)-b-poly(L-lactide) diblock copolymer (PEO-b-PLLA) is characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a block length distribution map is constructed. Although the MALDI- TOF mass spectrum of PEO-b-PLLA is very complicated, most of the polymer species were identified by isolating the overlapped isotope patterns and by fitting the overlapped peaks to the Schulz-Zimm distribution function. Reconstructed MALDI-TOF MS spectrum was nearly identical to the measured spectrum and this method shows its potential to be developed as an easy and fast analysis method of low molecular weight block copolymers.