• Journal of IKEEE
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• v.23 no.1
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• pp.266-272
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• 2019

Multicopters have become more popular since they are advantageous in their ability to take off and land vertically. In order to guarantee the normal operations of such multicopters, the problem of fault detection and isolation is very important. In this paper, a new method for detecting and isolating an actuator fault of a hexacopter is proposed based on the analytical approach. The residual is newly defined using the angular velocities of actuators estimated by the mathematical model and an actuator fault is detected comparing the residuals to a threshold. And a fault is isolated combining a dynamic model and generated residuals when a fault is detected. The proposed method is a simple, but effective technique because it is based on mathematical model. The results of the computer simulation are also given to demonstrate the validity of the proposed algorithm in case of a single failure.

• Structural Engineering and Mechanics
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• v.85 no.6
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• pp.825-835
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• 2023

The effective buckling length factor is an important parameter in the elastic buckling analysis of steel structures. The present article aims at developing a new method that allows the determination of the buckling factor values for frames. The novelty of the method is that it considers the interaction between the bracing and the elastic supports for asymmetrical frames in particular. The approach consists in isolating a critical column within the frame and evaluating the rotational and translational stiffness of its restraints to obtain the critical buckling load. This can be achieved by introducing, through a dimensionless parameter 𝜙_i, the effects of coupling between the axial loading and bending stiffness of the columns, on the classical stability functions. Subsequently, comparative, and parametric studies conducted on several frames are presented for assessing the influence of geometry, loading, bracing, and support conditions of the frame columns on the value of the effective buckling length factor K. The results show that the formulas recommended by different approaches can give rather inaccurate values of K, especially in the case of asymmetric frames. The expressions used refer solely to local stiffness distributions, and not to the overall behavior of the structure.

• Journal of Applied Biological Chemistry
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• v.57 no.3
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• pp.259-265
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• 2014

Mitochondrial DNA (mtDNA)-depleted (${\rho}^0$) cells are often used as mtDNA recipients to study the interaction between the nucleus and mitochondria in mammalian cells. Therefore, it is crucial to obtain mtDNA-depleted cells with many different nuclear backgrounds for the study. Here, we demonstrate a rapid and reliable method to isolate mammalian mtDNA-depleted cells involving treatment with the antimitochondrial agents ethidium bromide (EtBr) and 2',3'-dideoxycytidine (ddC). After a short exposure to EtBr or ddC, followed by rapid clonal isolation, we were able to generate viable mtDNA-depleted cells from mouse and human cells and were able to successfully repopulate them with exogenous mitochondria from platelets isolated from mouse and human blood samples. These mtDNA-depleted cells can be used to characterize the nuclear mitochondrial interactions and to study mtDNA-associated defects in mammalian cells. Our method of isolating mtDNA-depleted cells is practical and applicable to a variety of cell types.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.25 no.3
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• pp.191-198
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• 2015

Noise propagated through the ventilation openings of enclosures is actively controlled using an FIR filter. The enclosures considered in this paper are used for isolating noise due to machinery. This method is of limited use because of the ventilation openings through which most of noise is propagated. Feedforward control strategy is incorporated to minimize the acoustic power propagated through the openings. For the real-time implementation, although it is numerical study, the controller is implemented using an FIR filter. The acoustic transfer functions of the pressure on the openings of the enclosure to the primary source and to the secondary source are numerically calculated using the boundary element method. The performance analysis of the active control is conducted with the time-domain simulation using Matlab Simulink.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.6 no.11
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• pp.2764-2783
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• 2012

Network virtualization supports future Internet environments and cloud computing. Virtualization can mitigate many hardware restrictions and provide variable network topologies to support variable cloud services. Owing to several advantages such as low cost, high flexibility, and better manageability, virtualization has been widely adopted for use in network virtualization platforms. Among the many issues related to cloud computing, to achieve a suitable cloud service quality we specifically focus on network and performance isolation schemes, which ensure the integrity and QoS of each virtual cloud network. In this study, we suggest a virtual network platform that uses Xen-based virtualization, and implement multiple virtualized networks to provide variable cloud services on a physical network. In addition, we describe the isolation of virtual networks by assigning a different virtualized network ID (VLAN ID) to each network to ensure the integrity of the service contents. We also provide a method for efficiently isolating the performance of each virtual network in terms of network bandwidth. Our performance isolation method supports multiple virtual networks with different levels of service quality.

• Asian-Australasian Journal of Animal Sciences
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• v.25 no.7
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• pp.950-955
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• 2012

This study was conducted to determine how the isolation method of the porcine preantral follicles influenced the following follicular growth in vitro. Mechanical and enzymatical isolations were used for retrieving the follicles from prepubertal porcine ovaries, and in vitro-growth of the follicles and the expression of folliculogenesis-related genes were subsequently monitored. The enzymatic retrieval with collagenase treatment returned more follicles than the mechanical retrieval, while the percentage of morphologically normal follicles was higher with mechanical retrieval than with enzymatic retrieval. After 4 days of culture, mechanically retrieved, preantral follicles yielded more follicles with normal morphology than enzymatically retrieved follicles, which resulted in improved follicular growth. The mRNA expression of FSHR, LHR Cx43, DNMT1 and FGFR2 genes was significantly higher after culture of the follicles retrieved mechanically. These results suggest that mechanical isolation is a better method of isolating porcine preantral follicles that will develop into competent oocytes in in vitro culture.

• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.566-569
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• 2005

A novel purification method was developed for producing homoharringtonine from Cephalotaxus koreana, to guarantee high purity and yield. Our simple, efficient procedure for isolating and purifying homoharringtonine from C. koreanabiomass consisted of solvent extraction, synthetic adsorbent treatment, low-pressure chromatography, followed by high performance liquid chromatography (HPLC). The use of active clay treatment and silica gel low-pressure chromatography in the pre-purification process allowed for the rapid, efficient separation of homoharringtonine from interfering compounds and dramatically increased the yield and purity of crude homoharringtonine for high-performance liquid chromatography (HPLC) purification steps compared with alternative processes. Homoharringtonine could be obtained simply with high yield and purity from biomass using this purification method, while minimizing solvent use and the scale and complexity of HPLC operations for homoharringtonine purification. Purified homoharringtonine was identified and characterized.

• Research in Community and Public Health Nursing
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• v.19 no.1
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• pp.101-111
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• 2008

Purpose: This study was to analyze the trends of research on school health by examining papers related to school health. Method: This studt was analyzed all related papers published in journals concerning school health from 2000 to 2006. The data were processed statistically by frequency percentage and content analysis. Results: Among 463 papers in 6 journals related to school health. 'The Journal of the Korean Society of School Health' had the largest number of articles. Descriptive research design was the most frequently used research design, and students were the most frequent research target. Among research writers, the professor group was largest, and schools were the most frequently used place for research. The most common sampling method was convenient sampling. The most common research instrument was questionnaire survey. Parametric statistics were the main methods of data analysis. Among the themes of school health, the adoption of nursing process frames and assessment domains occupied the largest portion. Health was the most common key concept, and the mainly used theory in the journals was factor-isolating. Conclusion: Through the analysis of research trends, we expect a higher level of school health research.

• Animal cells and systems
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• v.7 no.2
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• pp.133-137
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• 2003

Secreted proteins and membrane proteins play key roles in the formation, differentiation, and maintenance of multicellular organisms. In this study, we undertook to characterize these protein types in the central nervous system of the marine snail Aplysia kurodai using a yeast-based signal sequence trap method. One hundred and three cDNA clones were obtained by screening 300,000 clones from the signal sequence trap cDNA library. Of these, twelve were identical to previously identified Aplysia genes, 19 were related to known proteins in other organisms, and 54 clones were novel. These 54 new genes had high signal peptide scores or were found likely to contain a transmembrane domain sequence. Only 18 of the 103 clones proved to be false positive. The study demonstrates that the signal sequence trap method is an effective tool for Isolating Aplysia genes encoding secreted and membrane proteins.

• Macromolecular Research
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• v.11 no.5
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• pp.341-346
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• 2003

A poly(ethylene oxide)-b-poly(L-lactide) diblock copolymer (PEO-b-PLLA) is characterized by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and a block length distribution map is constructed. Although the MALDI- TOF mass spectrum of PEO-b-PLLA is very complicated, most of the polymer species were identified by isolating the overlapped isotope patterns and by fitting the overlapped peaks to the Schulz-Zimm distribution function. Reconstructed MALDI-TOF MS spectrum was nearly identical to the measured spectrum and this method shows its potential to be developed as an easy and fast analysis method of low molecular weight block copolymers.