• Structural Monitoring and Maintenance
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• v.1 no.2
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• pp.213-230
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• 2014

A novel real-time actuator failure detection algorithm is developed in this paper. Actuator fails when the input to the structure is different from the commanded one. Previous research has shown that one error function can be formulated for each actuator through interaction matrix method. For output without noise, non-zero values in the actuator functions indicate the instant failure of the actuator regardless the working status of other actuators. In this paper, it is further demonstrated that the actuator's error function coefficients will be directly calculated from the healthy input of the examined actuator and all outputs. Hence, the need for structural information is no longer needed. This approach is termed as direct method. Experimental results from a NASA eight bay truss show the successful application of the direct method for isolating and identifying the real-time actuator failure. Further, it is shown that the developed method can be used for real-time sensor failure detection.

• Clinical and Experimental Reproductive Medicine
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• v.27 no.3
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• pp.235-243
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• 2000

Objective: Our present studies were conducted to examine more effective isolating method of preantral follicles from mouse ovaries. Methods: ICR mice (3-6 weeks old) were sacrificed through cervical dislocation and their ovaries were removed and put into watch glasses containing Hams F-10 supplemented with 10% fetal bovine serum (FBS). Preantral follicles were isolated by three different methods; 1) enzymatical method and 2) mincing method, and 3) scraping method. Enzymatical method was carried out as following. Ovaries were bisected with a pair of fine 30G needles. Bisected ovaries were incubated at $37^{\circ}C$ and 5% $CO_2$ incubator in 2-well dish containing Hams F-10 supplemented with collagenase 600 lU/ml and DNAse 20 lU/ml. After 20 min., follicles were isolated by repeated pipetting. Isolated preantral follicles were collected, and the remnant of tissues was placed in incubator and previous procedure was repeated. Mincing method was carried out with a pair of fine 30G needles attached to 1 ml syringes and minced ovary. Scraping method was carried out with a pair of fine 30G needles and scratched to surface of ovary. The differences between isolating methods were analyzed using Student's t-test and Chi-square. Results were considered statistically significant when ${\rho}$ value was less than 0.05. Results: In handling time, mincing or scraping method ($28{\pm}3.42$ min or $16{\pm}1.58$ min) were significantly (p<0.00001) shorter than enzymatical method ($72{\pm}1.69$ min), and scraping method was significantly (p<0.01) shorter than mincing method. Total number of isolated follicles was significantly (p<0.0001) higher in enzymatical method ($49.8{\pm}3.91$) than in mincing or scraping method ($25.3{\pm}2.33$ or $20.5{\pm}1.75$). Isolated follicles in ${\leq}$90${\mu}m$ were significantly (p<0.005) higher in enzymatical method ($15{\pm}1.71$) than in mincing or scraping method ($7.8{\pm}0.98$ or $8.1{\pm}1.31$). In 91~130 ${\mu}m$, isolated follicles were significantly (p<0.0005) higher in enzymatical method ($33{\pm}3.27$) than in mincing or scraping method ($16.3{\pm}1.82$ or $10.7{\pm}1.38$). In ${\geq}$ 131 ${\mu}m$, isolated follicles were not significantly differences between all groups. In equal sizes, the rate of isolated follicles in ${\leq}$ 90 ${\mu}m$ was highest in scraping method (39.6% vs. enzymatical method: 30.1%, p<0.05; mincing method: 30.9%, p=0.11719, NS). Rate of follicles in $91{\sim}130$ ${\mu}m$ was significantly (p<0.05) lower in scraping method (52.7%) than in enzymatical or mincing method (66.3% or 64.5%). Rate of follicles in ${\geq}$131 ${\mu}m$ was highest in scraping method (8.3% vs. enzymatical or scraping method: 3.6%, p<0.05 or 4.6%, p=0.19053, NS). Conclusions: This study suggests that scraping method is simple and useful for isolation of preantral follicles, because this method reduced handling time and recovered enough follicles. The recovered rate of isolated follicles in diameter of 91 ~ 130 ${\mu}m$ was highest in all methods.

• IEMEK Journal of Embedded Systems and Applications
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• v.3 no.3
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• pp.151-157
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• 2008

In this paper, we propose a three dimensional distance measurement method for a stereo system by using web cams. Using a parallel stereo system, a robot gets two images from each webcam and equalize brightness of both images. And we suggest an image processing method such as labeling, isolating an object from background and finding center of an object. We also propose a method of calculating the focal distance by using least square algorithm based on triangulation and we can reduce calculation error by this method. From experimental results, we show that the proposed method can be effective for 3D distance measurement.

• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.289-296
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• 1985

A simple procedure for rapid isolation of plasmid DNA from lactobacillus species and streptococcus species is described. Lactic acid bacteria were cultured in the TCM broth containing 0.5％ glycine and plasmid DNA was isolated from cells treated with mutanolysin by alkaline-detergent lysis method. Good results for releasing and isolating plasmid DNA from lactobacillus species were obtained by treatment of cells with 30$\mu\textrm{g}$ of mutanolysin per ml at 37$^{\circ}C$ for 5 to 10 min. For the streptococcus species, the optimum conditions were slightly different. The procedure could be used for rapid characterization of plasmid DNA in Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus helveticus, Streptococcus lactis, Streptococcus faecalis, Streptococcus faecium, and Streptococcus cremoris strains. Using this procedure, plasmids isolated from $1.5m\ell$ cultures could readily be visualized in agarose gel.

• Earthquakes and Structures
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• v.24 no.3
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• pp.217-235
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• 2023

Orthogonal pairs of rollers on concave beds (OPRCB) are a low-cost, low-tech rolling-based isolating system, whose high efficiency has been shown in a previous study. However, seismic performance of OPRCB isolators has only been studied in the two-dimensional (2D) state so far. This is while their performance in the three-dimensional (3D) state differs from that of the 2D state, mainly since the vertical accelerations due to rollers' motion in their beds, simultaneously in two orthogonal horizontal directions, are added up and resulting in bigger vertical inertia forces and higher rolling resistance. In this study, first, Lagrange equations were used to derive the governing equations of motion of the OPRCB-isolated buildings in 3D. Then, some regular shear-type OPRCB-isolated buildings were considered subjected to three-component excitations of far- and near-source earthquakes, and their responses were compared to those of their fixed-base counterparts. Finally, the effects of more realistic modeling and analysis were examined by comparing the responses of isolated buildings in 2D and 3D states. Response histories were obtained by the fourth-order Runge-Kutta-Nystrom method, considering the geometrical nonlinearity of isolators. Results reveal that utilizing the OPRCB isolators effectively reduces the acceleration response, however, depending on the system specifications and earthquake characteristics, the maximum responses of isolated buildings in the 3D state can be up to 40% higher than those in the 2D state.

• Journal of Information Display
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• v.6 no.1
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• pp.1-7
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• 2005

We propose new fabrication methods of a pixel-isolated liquid crystal (LC) structure for flexible display applications. In the LC structure fabricated through the proposed method, the patterned interpixel walls for sustaining the cell thickness are supported by the solidified polymer layer through anisotropic phase separation of LC/polymer composite, causing the alignment of the LC molecules to have very good mechanical stability against external pressure. In addition, we show that such pixel-isolating walls can be made by the stamping method which can be applied to fabricate large size plastic LCDs by roll-to-roll processing.

• Proceedings of the KSR Conference
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• 2000.11a
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• pp.157-164
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• 2000

This study is about predicting the interior pressure level of the korean high speed train using ray acoustic method. The motor car and the motor and passenger cabin are investigated under the environment of passing open countryside and inside tunnel of 350 km/hr. Calculated sound levels are compared with the proposed sound levels and suggestions about the transmission Joss values of isolating panels inside motor car and the guide lines of allowed sound power limit of motor equipments are provided. Results of TPI car show calculated interior sound level is below the proposed values for both cases of open countryside running and inside tunnel. Since ray acoustic method calculated only air borne noise component, real sound level of the motor car may be higher than prediction. Passenger cabins of TMI, TM5 show higher sound level than the proposed values, so window method was carried out to find the contribution of each panel components and point out the remedy of transmission path. Reduction of sound power of motor equipments should be condisered at the same time.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.5 no.8
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• pp.1423-1443
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• 2011

Background compensation plays an important role in detecting and isolating object motion in visual tracking. Here, we propose a Genetic Hough Transform, which combines the Hough Transform and Genetic Algorithm, as a method for eliminating background motion. Our method can handle cases in which the background may contain only a few, if any, feature points. These points can be used to estimate the motion between two successive frames. In addition to dealing with featureless backgrounds, our method can successfully handle motion blur. Experimental comparisons of the results obtained using the proposed method with other methods show that the proposed approach yields a satisfactory estimate of background motion.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.03a
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• pp.97-104
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• 2008

Most of engine control systems for helicopter turboshaft engines are equipped with dual sensors. For the system with dual redundancy, analytic methods are used to detect faults based on the system dynamical model. Helicopter engine dynamics are affected by aerodynamic torque induced from the dynamics of the main rotor. In this paper an engine model including the rotor dynamics is constructed for the T700-GE-700 turboshaft engine powering UH-60 helicopter. The singular value decomposition(SVD) method is applied to the developed model in order to detect sensor faults. The SVD method which do not need an additional computation to generate residual uses the characteristics that the system outputs in direction of the left singular vector if an input is applied in direction of the right singular vector. Simulations show that the SVD method works well in detecting and isolating the sensor faults.

• Korean Management Science Review
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• v.13 no.2
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• pp.1-11
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• 1996

Two different methods to extract the license plate area of a vehicle have been used for automatic recognition purposes. One method is with a color vision system and the other is with an edge detecting operator. The system with color vision has some problems if the colors of license plate and vehicle's body are similar. The various plate colors in Korea also drops the system performance. The edge detecting operator also has a problem for a real time processing since it performs on all pixels of the scene. In this paper a possible method using gray level vision system and available pre-known information of license plates is suggested. The suggested procedure searches the lower boundary of the plate by counting high contrast points between one and near pixel from the bottom line of the scene. It finds the upper boundary from the bottom line by adding number plate height after finding the lower boundary. The left and right boundaries are found by similar processes.