• Journal of applied mathematics & informatics
• /
• 제20권1_2호
• /
• pp.557-567
• /
• 2006

This paper proves the invariability of reachable sets for the linear control system with positive isolated spectrum points in case where the principal operator generates $C_0-semigroup$ and derives the approximate controllability for the semilinear control system by using spectral operators with respect to isolated spectrum points.

• Applied Biological Chemistry
• /
• 제16권1호
• /
• pp.12-17
• /
• 1973

냉동시킨 마늘로부터 Alliin을 분리(分離)해서 순수한 상태(相態)로 결정화(結晶化) 시켰다. 분리(分離)된 Alliin의 순도(純度)는 융점측정과 thin-layer chromatography에 의하여 확증되었다. Sulfoxide bond 및 아미노산의 기능기는 IR spectrum에 의하여, vinyl 결합은 NMR spectrum에 의하여 확인되었다. 분자량, allyl 및 기타의 결합은 MS spectrum에 의하여 확인(確認)되었다.

• 약학회지
• /
• 제42권6호
• /
• pp.552-557
• /
• 1998

Acyl CoA synthetase inhibitor, was purified from the culture broth of a Bacillus sp. B-6, which had been isolated from soil, by chloroform extract, silica gel column chro matography and preparative TLC. The purified acyl CoA synthetase inhibitor showed higher Antifungal activity against C. al-bicans (MIC: 8${\mu}$g/ml). Though the analysis of UV spectrum, melting point, IR spectrum, Mass-spectrum, $^1H$-NMR and $^{13}C$-NMR spectrum, the inhibitor could be identified as phenazine-l-carboxylic acid.

• Earthquakes and Structures
• /
• 제14권1호
• /
• pp.33-44
• /
• 2018

Base-isolated nuclear power plant (BI-NPP) structures are founded on expanded basemat as a flexible floating nuclear island, are still lacking the recommendation of the consideration of incoherent motion effect. The effect of incoherent earthquake motion on the seismic response of BI-NPP structure has been investigated herein. The incoherency of the ground motions is applied by using an isotropic frequency-dependent spatial correlation function to perform the conditional simulation of the reference design spectrum compatible ground motion in time domain. Time history analysis of two structural models with 486 and 5 equivalent lead plug rubber bearing (LRB) base-isolators have been done under uniform excitation and multiple point excitation. two different cases have been considered: 1) Incoherent motion generated for soft soil and 2) Incoherent motion generated for hard rock soil. The results show that the incoherent motions reduce acceleration and the lateral displacement responses and the reduction is noticeable at soft soil site and higher frequencies.

• 생명과학회지
• /
• 제20권5호
• /
• pp.676-682
• /
• 2010

본 연구는 임상검체만이 아닌 주변 생활환경에도 광범위 베타 락탐분해효소를 생성하는 균주(extended-spectrum $\beta$-lactamase, ESBL)가 존재하는지를 확인하고 만약 존재할 경우 그 균주를 분리하고 ESBL유형을 알아보기 위하여 실시되었다. 부산 광안리 하수처리 방류수에서 이중 디스크 확산 검사 결과 양성반응을 나타낸 29균주를 선별하였다. 이중 sodium azide에 내성을 가진 피전달 균주인 Escherichia coli J5에 교차접합이 이루어진 15균주를 대상으로 indole, methyl-red, Voges-Proskauer, Simmon's citrate 시험과 decarboxylase-dihydrolase 및 여러 종류의 당 발효 시험 등 생화학 검사를 실시한 결과 Klebsiella pneumoniae(13균주)와 Escherichia coli(2균주)가 동정되었다. 등전점, PCR, 유전자서열 분석을 실시하여 ESBL 유형을 결정하였다. Klebsiella pneumoniae의 ESBL 생성유형은 SHV-12(4균주)와 SHV-12/TEM-1(9균주)의 2종류로 구분되었고, Escherichia coli의 ESBL 생성유형은 TEM-1(2균주)로 판명되었다.

• Journal of Pharmaceutical Investigation
• /
• 제6권2호
• /
• pp.111-113
• /
• 1976

Rutin was isolated from the leaves of Sambucus latipinna var. coreana and identified by comparison of its mixed melting point and IR spectrum against the authentic sample.

• 생약학회지
• /
• 제7권3호
• /
• pp.209-210
• /
• 1976

To identify eriodictyol from the leaves of Lespedeza maximowiczii C.K. $S_{CHNEIDER}$, growing in Korea, the fresh leaves collected at Mt. Chil Bo, Geong Gi Province were used in the investigation. A white crystal was isolated from the water extract and it was identified as eriodictyol by its melting point, physico-chemical properties, thin layer chromatography and infrared absorption spectrum patterns.

• Archives of Pharmacal Research
• /
• 제24권6호
• /
• pp.590-596
• /
• 2001

A new extended-spectrum ${\beta}-lactamase$ with an isoelectric point (pl) of 6.2 was detected in Klebsiella pneumoniae Fl 61 that was isolated from a patient with infection. This strain was highly resistant to the third or fourth generation cephalosporins such as cceftazidime ceftriaxone, cefoperzaone, and cefpirome. Analysis of this strain by the double disk diffusion test showed synergies between amoxicillin-clavulanate (AMX-CA) and cefotaxime, and AMX-CA and aztreonam, which suggested that this strain produced a extended-spectrum ${\beta}-lactamase$ (ESBL). Cenetic analysis revealed that the resistance was due to the presence of a 9.4-kb plasmic, designated as pkpl 61, encoding for new ${\beta}-lactamase$ gene (bla). Sequence analysis showed that a new bla gene of pkpl 61 differed from $bla_{TEM-1}$ by three mutations leading to the following amino acid substitutions: $Val_{84}{\rightarrow}lie,{\;}Ala_{184}{\rightarrow}Val,{\;}and{\;}Gly_{238}{\rightarrow}Ser$. These mutations have not been reported previously in the TIM type ${\beta}-lactamases$ produced by clinical strains. The novel ${\beta}-lactamase$ was overexpressed in E. coli and purified by ion exchange chromatography on Q-Sepharose and CM-Sepharose, and then further purified by gel filtration on Sehadex G-200. The catalytic activity of th8 purified ${\beta}-lactamase$ was confirmed by the nitrocefin disk.

• 한국미생물·생명공학회지
• /
• 제19권6호
• /
• pp.604-609
• /
• 1991

토양에서 분리한 Streptomyces nigrifaciens GMT-497호무터 G(+) bacteria에만 강한 항균활성을 갖는 항생물질 MT-497을 용매추출, silica column chromatography와 재결정화를 통하여 분리 정제하였다. MT-497의 UV, 융점, 원소분석, IR spectrum, $^1H-NMR$과 구성아미노산 분석을 통해 actinocin chromophore와 threonine, proline, methyl valine, sarcosine, aspartic acid로 구성된 peptide을 갖는 actinomvcin계열의 항생물질로 동정하였다.

• 한국미생물·생명공학회지
• /
• 제22권2호
• /
• pp.169-174
• /
• 1994

Antitumor antibiotic HS-1 was purified from the culture broth of a streptomyces floridae SHS-1372 which had been isolated from soil, by solvent extraction, silica gel column chromatography and gel filtration. It was confirmed that HS-1 was active against gram positive bacteria and cancer cells(K562, P388, MCF-7, HT-29). Through the analysis of UV spectrum, melting point, IR spectrum, FAB-MS, $_{1}$H-NMR, $_{13}$C-NMR, 2D-NMR spectra, HS-1 could be identified sa the actinomycin X$_{2}$ antibiotics containing actinocine chromophore and peptides consisted of threonine, proline, methylvaline, sarcosine, D-valine and 4-ketoproline.