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http://dx.doi.org/10.5352/JLS.2010.20.5.676

Types of Extended-Spectrum β-Lactamase Produced in Enteric Bacteria Isolated from Sewage Plant Drain Water  

Kim, Gun-Do (Department of Microbiology, College of Natural Sciences, Pukyong National University)
Lee, Hun-Ku (Department of Microbiology, College of Natural Sciences, Pukyong National University)
Publication Information
Journal of Life Science / v.20, no.5, 2010 , pp. 676-682 More about this Journal
Abstract
This study focused on typing of the extended-spectrum $\beta$-lactamase (ESBL) produced in organisms isolated from a natural environment, rather than a clinical setting. Samples were collected from drain water issuing from a sewage plant in Kwanganri (Busan, Korea). Following double disk synergy testing, 29 strains were selected as potential ESBL positive strains. Of these, 15 strains were transconjugants of the sodium azide resistant recipient strain Escherichia coli J53 and analyzed biochemically including indole, methyl-red, Voges-Proskauer, Simmon's citrate, decarboxylase-dihydrolase and sugar-fermentation tests. The tests classified the 15 strains as Klebsiella pneumoniae (n=13) and Escherichia coli (n=2). The type of ESBL from each strain was deduced by isoelectric focusing point analysis and DNA sequencing. The results indicated that the types of ESBL were SHV-12 (n=4) and SHV-12/TEM-1 (n=9) from K. pneumoniae and TEM-1 (n=2) from E. coli strains.
Keywords
Extended-spectrum $\beta$-lactamase (ESBL); SHV-12; TEM-1; Klebsiella pneumoniae; Escherichia coli;
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