• Title/Summary/Keyword: investigation sources

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Antimicrobial Resistance Characteristics of Gram-Negative Bacteria Isolated from Inland Pollution Sources in the Drainage Basin of Iwon-myeon (Taean-gun), South Korea (태안군 이원면 육상오염원 배출수에서 분리한 그람음성균의 항생제 내성 특성)

  • Park, Bo Mi;Kim, Min Ju;Jeong, Yeon Gyeom;Park, Jin Il;Yu, Hong Sik;Oh, Eun Gyoung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.4
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    • pp.377-387
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    • 2021
  • Fecal contamination levels of discharge water from inland pollution sources were investigated in Iwon-myeon (Taean-gun), South Korea. Gram-negative bacteria were isolated during the investigation and the antimicrobial resistance patterns of the isolates were examined to estimate their impact on the coastal environment. The ranges of total coliform and fecal coliform of 12 samples from four major inland pollution sources were 79-490,000 MPN/100 mL and 2.0-490,000 MPN/100 mL, respectively, with the highest level of fecal contamination at Station No. 3. A total of 137 strains (14 genus) were isolated, of which 86 strains (62.8%) were Enterobacteriaceae. The identified isolates were as follows: Pseudomonas spp. (35 strains), Klebsiella spp. (20 strains), Serratia spp. (20 strains), and Escherichia spp. (19 strains). The isolated Gram-negative bacteria showed the highest antimicrobial resistance to ampicillin (81.8%), followed by amoxicillin/clavulanic acid (64.2%), ceftiofur (61.3%), and cefoxitin (59.1%). Antimicrobials in which less than 10% of isolates showed antimicrobial resistance were ciprofloxacin (3.6%) and gentamicin (2.2%). Resistance to one or more antimicrobials was observed in 121 strains (88.3%) and 84 strains (61.3%) showed a tendency for multiple antimicrobial resistance.

An Exploratory Study on Purchase Decision Making Process and Clothing Shopping Orientation of Fashion Products Rental Service Users (패션제품 대여 서비스 이용자의 구매의사결정과정과 의복 쇼핑성향에 관한 탐색적 연구)

  • Lee, Ji-Yoon;Shin, Eun-Jung;Koh, Ae-Ran
    • Human Ecology Research
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    • v.56 no.6
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    • pp.555-571
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    • 2018
  • This study identified the characteristics of fashion rental service users as well as analyzed their purchase decision-making processes. A qualitative investigation was conducted through in-depth interviews with 13 women in their 20s-30s who have experienced renting fashion items due to a high interest in fashion. The results of the study are summarized as follows. The need recognition stage analyzed ventilation by mass media, SNS impact, curiosity, saving shopping time and money, awareness of situational necessity, and creation of various styles. The information search stage analyzed how users obtained information from 2 different sources of nonmarketer-dominated sources and marketer-dominated sources. The pre-purchase stage analyzed the evaluation of alternatives in which study participants used 2 evaluation criteria for fashion rental services and fashion rental items. The purchase stage analyzed how participants wait and select desired items (when receiving the notification of rentable items) or select alternative products. The consumption stage examined the usage frequency and usage method. The study divided the post-consumption evaluation stage into 2 categories for evaluation: personal feelings and service. The post-consumption behavior stage analyzed how participants displayed WOM, eWOM and purchase rental product behavior. Clothing shopping orientation of study participants is displayed in 5 dimensions of brand-seeking propensity, individuality-seeking propensity, economic efficiency-seeking propensity, rationality-seeking propensity, and pleasure-seeking propensity. This study identified three main characteristics in the study participants: interest in the fashion, favorable attitude toward used fashion items, consciousness of others.

Nutritional composition of various insects and potential uses as alternative protein sources in animal diets

  • Shah, Assar Ali;Totakul, Pajaree;Matra, Maharach;Cherdthong, Anusorn;Hanboonsong, Yupa;Wanapat, Metha
    • Animal Bioscience
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    • v.35 no.2_spc
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    • pp.317-331
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    • 2022
  • The aim of the present investigation is to determine the nutritional composition of various insects and their potential uses as alternative protein sources in animal diets. The feeding industry requires production systems that use accessible resources, such as feed resources, and concentrates on the potential impacts on production yield and nutritional quality. Invertebrate insects, such as black soldier flies, grasshoppers, mealworms, housefly larvae, and crickets, have been used as human food and as feed for nonruminants and aqua culture while for ruminants their use has been limited. Insects can be mass-produced, participating in a circular economy that minimizes or eliminates food- and feed-waste through bioconversion. Although the model for formula-scale production of insects as feed for domestic animals has been explored for a number of years, significant production and transformation to being a conventional protein resource remains to be deeply investigated. This review will focus on the nutritional composition of various insects and their potential use as alternative protein sources, as well as their potential use to promote and support sustainable animal production. Furthermore, nutritional compositions, such as high protein, lauric acid omega 6, and omega 3, and bioactive compounds, such as chitin, are of great potential use for animal feeding.

Sources, Components, Structure, Catalytic Mechanism and Applications: a Critical Review on Nicotinate Dehydrogenase

  • Zhi Chen;Xiangjing Xu;Xin Ju;Lishi Yan;Liangzhi Li;Lin Yang
    • Journal of Microbiology and Biotechnology
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    • v.33 no.6
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    • pp.707-714
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    • 2023
  • Plant-derived insecticide-neonicotinoid insecticides (NIs) played a crucial role in the development of agriculture and food industry in recent years. Nevertheless, synthesis of these nitrogen-containing heterocyclic compounds with an effective and greener routing remains challenging especially to the notion raise of "green chemistry" and "atom economy". While bio-catalyzed methods mediated by nicotinate dehydrogenase (NDHase) then provide an alternative. The current review mainly focuses on the introduction of sources, components, structure, catalytic mechanism and applications of NDHase. Specifically, NDHase is known as nicotinic acid hydroxylase and the sources principally derived from phylum Proteobacteria. In addition, NDHase requires the participation of the electron respiratory chain system on the cell membrane. And the most important components of the electron respiratory chain are hydrogen carrier, which is mainly composed of iron-sulfur proteins (Fe-S), flavin dehydrogenase (FAD), molybdenum binding protein and cytochromes. Heterologous expression studies were hampered by the plasmid and host with high efficiency and currently only Pseudomonas entomophila L48 as well as Comamonas testosterone was successfully utilized for the expression of NDHase. Furthermore, it is speculated that the conjugate and inductive effects of the substituent group at position 3 of the substrate pyridine ring exerts a critical role in the hydroxylation reactions at position 6 concerning about the substrate molecular recognition mechanism. Finally, applications of NDHase are addressed in terms of pesticide industry and wastewater treatment. On conclusion, this critical review would not only deepen our understanding of the theory about NDHase, but also provides the guideline for future investigation of NDHase.

Information Poverty Viewed Through the Families of Children With Developmental Disabilities (발달장애아 가족의 정보빈곤 양상에 관한 연구)

  • Seo, Ok-Youn;Chang, Durk-Hyun
    • Journal of the Korean BIBLIA Society for library and Information Science
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    • v.19 no.2
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    • pp.107-128
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    • 2008
  • The purpose of this study is to portray the phenomena of information poverty of the families of children with developmental disabilities. The focus was especially put on investigating their information needs of everyday lives and information-seeking behaviors. The sample group includes six children with developmental disabilities and their families, mainly mothers. The investigation was conducted by the participant observation and in-depth interviews. The study found out that they sought the formal information sources such as medical specialists and special agencies first with which they were not satisfied. As a result, they rather relied on informal information sources. And the information from 'the group of families of children with developmental disabilities' was thought to be the most reliable information for them. In terms of information related behaviors, the strongest information needs were regarding the children's special education, especially to the school education, and they made effort to seek reliable information. They, however, tended to passively treat their personal concerns that are not related to their disabled children. This study found out that their information poverty is in terms of the information environment, information sources and their attitude to information, and that it comes from the external(social) factors as well as internal(individual) ones.

Scientific fire investigation by NFPA 921 CODE based on frozen warehouse fire case (냉동창고 화재 사례를 기반으로 하는 NFPA 921 CODE에 의한 과학적 화재조사 연구)

  • Park, Kyong-Jin;Lee, Yong-KI;Cha, Sung-Sig;Jung, Dong-Young;Kim, Jang-Oh
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.8
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    • pp.78-85
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    • 2018
  • In this study, we investigated the cases where there were many opinions in the judgment of the cause of ignition in the case of 20 cases of frozen warehouse fire that occurred in 2017.The research methodology is the scientific fire survey method prescribed by the NFPA 921 CODE. Scientific fire investigation method is fire investigation method by logical reasoning through hypothesis setting, minimizing errors in judgment of ignition source. On the other hand, unscientific fire investigation methods cause many errors by the intervention of irrational factors such as subjective estimation, reasoning judgment, etc. This eventually leads to the problem of human and material responsibility and academic deterioration. In particular, fire not seen as compared to sighted fire makes more errors in ignition sources in the cause investigation. In this study, we set the hypothesis A and hypothesis B based on the review of the fire investigation report and the field survey on the fire case of the cold storage warehouse front line that occurred at ** city ** Mart in 2017.The set hypothesis was tested by the NFPA 921 code. This analytical method will be constructed by NEW Paradigm as a source of fire that is not seen in the future and a source of ignorant fire.In addition, the experimental data of this study will be used to inform the manufacturer and operator of the refrigeration warehouse and serve as basic data for fire prevention.

Rapid Detection of Bacteria from Blood Culture by an Electronic Nose

  • Lykos, Peter;Patel, Pravin H.;Morong, Christopher;Joseph, Asha
    • Journal of Microbiology
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    • v.39 no.3
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    • pp.213-218
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    • 2001
  • The treatment of Patients with bacteraemia and septicemia requires accurate and rapid identification of the pathogen so that the physician can be guided regarding the selection of the proper antimicrobial therapy. The usual procedure is to withdraw an aliquot of the positive blood culture sample for gram staining and subculturing on the media for the growth and subsequent identification, and susceptibility determinations. It was noticed that during the process some microbiologists would sniff the effluent gases that are products of metabolism and in some cases guess the identity of the bacterium. That Prompted us to engage in systematic investigation of two gram positive and two gram negative bacteria using an electronic nose that had been proven successful in distinguishing the aroma of coffee beans from different sources. The investigation was successful in illustrating the efficacy of such a device in this clinical setting to distinguish Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. A representative set of patterns obtained with this apparatus is displayed as well. A representative set of patterns obtained with this apparatus is displayed as well. No effort was made to determine an optimal set of sensors for some specific set of bacterial metabolism gaseous products.

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Biochemical Characterization of Protease Produced by Cordyceps nutans

  • Kim, Seon Ah;Kim, Mi-Kyung
    • Korean Journal of Clinical Laboratory Science
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    • v.44 no.4
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    • pp.216-221
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    • 2012
  • The fruiting body of Cordyceps is derived from the pupa or larva of insects infected by the entomopathogenic fungi Cordyceps. The fruiting body has been used as an anti-cancer and anti-inflammatory ingredient in traditional Chinese medicine. The biochemical characteristics of protease isolated from Cordyceps nutans were investigated in this study. The culturing period for production of protease by C. nutans was 10days. The acidity was pH 7.0, and the temperature was $25^{\circ}C$. The carbon and nitrogen sources for the production of the protease were glucose and yeast extract, respectively. The ratio of C/N was 2% glucose and 0.6% yeast extract. 0.06% $CuSO_4$ was used as the inorganic salt. The investigation into the acidity of the protease produced by C. nutans revealed that the optimal pH and temperature were pH 7.0 and $30^{\circ}C$. The stability of the protease was shown as pH 6.0~9.0 and $30{\sim}50^{\circ}C$. The investigation into the influence of the metal ions on the enzyme activation of C. nutans revealed that it was inhibited in $ZnSO_4$ and activated in $FeSO_4$.

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Commercial Database : The Keypoints and Practical Use(2) - Newspaper Articles - (상용(商用) 데이터베이스 : 요점(要點)과 활용(活用)(2) - 신문기사(新聞記事) -)

  • Cho, Jae-Ho
    • Journal of Information Management
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    • v.24 no.3
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    • pp.31-56
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    • 1993
  • In order to respond to the public needs to utilize the newspapers as high-valued information resources, the number of the commercially available news items which are transformed into a form of database has been remarkably increased by the introduction of CTS into the newspaper companies. As for the domestically available Japanese and foreign newspaper article database, explanations are given to the following items. 1) Characteristics of database, such as date of coverage, frequency updating, time-lag, news sources, search terms, available information, etc. 2) Matters to be noticed at the utilization. 3) Pattern of utilization depending on the object of the investigation, such as the investigation of company, market trend, etc. Since the newspaper article database will be available in a form of digitized information, making the combination with the inter-company LAN system possible, increased opportunities to use it in near future are expected.

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Durability performance of concrete containing Saudi natural pozzolans as supplementary cementitious material

  • Al-Amoudi, Omar S. Baghabra;Ahmad, Shamsad;Khan, Saad M.S.;Maslehuddin, Mohammed
    • Advances in concrete construction
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    • v.8 no.2
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    • pp.119-126
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    • 2019
  • This paper reports an experimental investigation conducted to evaluate the durability performance of concrete mixtures prepared utilizing blends of Type I Portland cement (OPC) and natural pozzolans (NPs) obtained from three different sources in Saudi Arabia. The control concrete mixture containing OPC alone as the binder and three concrete mixtures incorporating NPs were prepared keeping water/binder ratio of 0.4 (by weight), binder content of $370kg/m^3$, and fine/total aggregate ratio of 0.38 (by weight) invariant. The compressive strength and durability properties that included depth of water penetration, depth of carbonation, chloride diffusion coefficient, and resistance to reinforcement corrosion and sulfate attack were determined. Results of this study indicate that at all ages, the compressive strength of NP-admixed concrete mixtures was slightly less than that of the concrete containing OPC alone. However, the concrete mixtures containing NP exhibited lower depth of water penetration and chloride diffusion coefficient and more resistance to reinforcement corrosion and sulfate attack as compared to OPC. NP-admixed concrete showed relatively more depth of carbonation than OPC when subjected to accelerated carbonation. The results of this investigation indicates the viability of utilizing of Saudi natural pozzolans for improving the durability characteristics of concrete subjected to chloride and sulfate exposures.