Rapid Detection of Bacteria from Blood Culture by an Electronic Nose

  • Lykos, Peter (Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology) ;
  • Patel, Pravin H. (Microbiology Laboratory, Department of Clinical and Anatomic Pathology, Provident Hospital of Cook Country) ;
  • Morong, Christopher (Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology) ;
  • Joseph, Asha (Department of Biological, Chemical and Physical Sciences, Illinois Institute of Technology)
  • Published : 2001.09.01

Abstract

The treatment of Patients with bacteraemia and septicemia requires accurate and rapid identification of the pathogen so that the physician can be guided regarding the selection of the proper antimicrobial therapy. The usual procedure is to withdraw an aliquot of the positive blood culture sample for gram staining and subculturing on the media for the growth and subsequent identification, and susceptibility determinations. It was noticed that during the process some microbiologists would sniff the effluent gases that are products of metabolism and in some cases guess the identity of the bacterium. That Prompted us to engage in systematic investigation of two gram positive and two gram negative bacteria using an electronic nose that had been proven successful in distinguishing the aroma of coffee beans from different sources. The investigation was successful in illustrating the efficacy of such a device in this clinical setting to distinguish Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Enterococcus faecalis. A representative set of patterns obtained with this apparatus is displayed as well. A representative set of patterns obtained with this apparatus is displayed as well. No effort was made to determine an optimal set of sensors for some specific set of bacterial metabolism gaseous products.

Keywords

References

  1. Biosens.Bioelectron. v.14 Flow-through immunofiltration assay system for rapid detection of E.coli O157:H7 Abdel-Hamid I.;D.Ivnitski;P.Atanasov;E.Wilkins
  2. Sensors Actuators B v.4 Chemical sensing,molecular electronics and nanotechnology: Interface technologies down to the molecular scale Goepel W.
  3. Biosens.Bioelectron. v.10 Interface analysis in biosensor design Goepel W.;P.Heduschka
  4. IEEE Spectrum
  5. IPRO Report,Electronic Nose
  6. Computer software applications in chemistry Peter J.
  7. Specimen Processing Manual of clinical microbiology(7th ed.) Reisner B.S.;G.L.Woods;R.B.Johnson Jr.;D.H.Larone;L.S.Garcia;R.Murray(ed.);E.J.Baron(ed.);M.A.Pealler(ed.);E.C.Tenover(ed.);R.H.Yolken(ed.)
  8. Biosens.Bioelectron. v.9 Transducer aspects of biosensors Sethi R.S.