• Title/Summary/Keyword: inv

Search Result 90, Processing Time 0.033 seconds

Molecular Taxonomy based on 16S rDNA Analysis and Pathogenicity of Yersinia pseudotuberculosis Isolated from Spring Waters (약수에서 분리한 Yersinia pseudotuberculosis의 병원성과 16S rDNA 분석에 의한 분자학적 분류)

  • Lee, Young-Kee;Choi, Sung-Min;Oh, Soo-Kyung;Lee, Kang-Moon;Ryeom, Kon
    • Korean Journal of Microbiology
    • /
    • v.37 no.1
    • /
    • pp.9-14
    • /
    • 2001
  • In order to investigate the pathogenicity and development of differential identification technique in the Yersinia species and other entericbacteria, we isolated 5 strains of Y.pseudotuberculosis from spring water sites in Seoul. The biochemical characteristics of isolated strains revealed that indole, VP($25^{\circ}C$, $37^{\circ}C$), $H_2S$, phenylalanine, lysine, arginine, ornithine, gas from glucose, lactose, sucrose, sorbitol, oxidase and motility($37^{\circ}C$) were all negative and urease, glucose, mannitol, salicin, catalase and motility($25^{\circ}C$) were all positive. To detect the causative agent of pseudotuberculosis(Y.pseudotuberculosis), we carried out a study using a PCR with inv primers complementary to the pathogenic region and found that all strains were positive, this revealed that strains from spring waters were pathogenic. Also 16S rDNA for total 5 strains of Y. pseudotuberculosis were amplified and a stretch of approximately 1,450 nucleotides were sequenced and analyzed. The 16S rDNA nucleotide sequence homologies among Yersinia species ranged 97.5% to 100% and between Y.pseudotuberculosis and other entericbacteria they ranged 93.0% to 95.1%. The Phylogenetic tree generated from the sequence analysis of the 16S rDNA gene showed 3 coherent clusters that could be separated into Y.pseudotuberculsis strains, some Yersinia species strains and other entericbacteria strains.

  • PDF

ON THE SPECTRAL RADIUS AND INVERTIBILITY OF CERTAIN ELEMENTS IN BANACH ALGEBRA

  • Park, Kyon-Hong;Kim, Byung-Do
    • Journal of applied mathematics & informatics
    • /
    • v.4 no.1
    • /
    • pp.299-308
    • /
    • 1997
  • In this paper we show that the limit of a convergent in-vertible sequence in the set of invertible elements Inv(A) in a Banach algebra A under a certain conditions is invertible and we investigate some properties of the spectral radius of banach algebra with unit.

A Prenatal Case of Paracentric Inversion of Chromosome 18, inv(18)(q21.1q22)

  • An, Gye-Hyeong;Kim, Moon Young;Kim, Min Hyoung;Kim, Yun Young;Choi, Kyu Hong;Kwak, Dong Wook;Park, So Yeon;Lee, Bom Yi;Park, Ju Yeon;Ryu, Hyun Mee
    • Journal of Genetic Medicine
    • /
    • v.9 no.2
    • /
    • pp.101-103
    • /
    • 2012
  • Paracentric inversion of chromosome 18 is a rare cytogenetic abnormality. The vast majority of paracentric inversions are harmless and the offspring of paracentric inversion carriers have only slightly elevated risks for unbalanced karyotypes. However, various clinical phenotypes are seen due to breakpoint variation or recombination. We report a prenatally detected case of familial paracentric inversion of chromosome 18, inv(18)(q21.1q22), with normal clinical features.

Application of Moment Tensor Inversion to Small Local Earthquakes in the Korean Peninsula (한반도의 소규모지진 모멘트 텐서 역산의 응용)

  • Kim, So-Gu;Van, Phan Thi Kim;Lee, Seoung-Kyu
    • Journal of the Korean Society of Hazard Mitigation
    • /
    • v.1 no.3 s.3
    • /
    • pp.123-136
    • /
    • 2001
  • The purpose of application of moment tensor inversion method is to determine source parameters, such as, focal mechanism, seismic moment and source depth. This paper presents results of focal mechanism solutions of 14 recent events with magnitudes ranging from M3.3 to M4.8 by using moment tensor inversion method called TDMT_INV. The strike of faults is in the direction of NE-SW and NW-SE with the movement of strike-slip or strike-slip of minor reverse component. The compressional axis of the stress field is predominantly E-W or ENE-WSW except for some faults, which are distributed at Ryongnam Massif and Wonsan, they have a compressional axis of N-S or NNW-SSE.

  • PDF

The Index of Nausea, Vomiting, and Retching(Korean Translation) (오심, 구토, 구역질 측정도구의 신뢰도와 타당도 검증)

  • Kim, Young Jae;Kim, Ji Young;Choi, In Ryoung;Kim, Mi Won;Rhodes, Verna
    • Korean Journal of Adult Nursing
    • /
    • v.12 no.2
    • /
    • pp.278-285
    • /
    • 2000
  • Nausea, vomiting and retching are universal symptoms that affect individuals' state of health and self-care activities of individuals. Accurate measurements of individual symptoms are required to gather more definitive data, and enhance understanding, planning, and implementation of self-care actions. Recently the Rhodes Index of Nausea, Vomiting, and Retching(INVR), a new format of the INV-2(the Rhodes Index of Nausea and Vomiting), was developed to measure the symptoms of nausea, vomiting and retching in an English speaking population. To determine the reliability and validity of the INVR, and the possibility of using the instrument in Korea, the Korean translation of the INVR and the INV-2 were administered to a convenient sample of 105 patients at two University Hospitals in Kwangju, Korea. The Cronbach's alpha to estimate the internal consistency of reliability for INVR was 0.844. Equivalent measures of reliability were conducted to determine the percentage of agreement and the Spearman rank correlation coefficients for responses on the two instruments. The percent agreement was 83% and the correlation coefficient was 0.906 over all. A significant differences between the INVR scores of the patients with and without nausea, vomiting, or retching were seen, which indicated a construct validity. The INVR was found to be more user friendly for the patient and the healthcare providers. As a result, it is suggested that the INVR can provide a scientific base for measuring the symptoms of nausea, vomiting, and retching for nurses to improve patients' care and quality of life.

  • PDF

A Case of Short Arm Deletion and Long Arm Duplication at Chromosome 3 (3번 염색체 단완 결실과 장완 중복을 동반한 1례)

  • Kong, Seung Hyun;Seo, Jeong Il;Kang, Jang Hui;Jung, So Young;Mok, Ji Sun
    • Clinical and Experimental Pediatrics
    • /
    • v.48 no.12
    • /
    • pp.1389-1389
    • /
    • 2005
  • The long arm duplication of chromosome 3 was reported for the first time in 1966 by Falek et al., and Hirschhorn et al. came to identify the duplication of 3q21${\rightarrow}$qter region in 1973. In most cases of duplication 3q syndrome patients, pure duplication of 3qter is believed to be rare and is often reported accompanied with deletion of another segment of the chromosome. Approximately 75 percent of parents of the patient in the meantime have been demonstrated to have unbalanced translocations or inversions of the chromosome. Partial deletion of the distal part of the short arm of chromosome 3 was first reported by Verjaal and De Nef in 1978 and terminal deletion of chromosome 3 (3p25-qter) has been observed in most cases. In karyotyping of chromosomes of immature infants showing the manifestations of flat occiputs, low set ears, hypertelorism, broad nasal roots, thin lips, web necks, hypotonia, hypertrichosis skin, cryptorchidism etc, we experienced a case diagnosed as 46,XY, rec(3)dup(3)(q21)del(3)(p25)inv(3)(p25q21).

Analysis of Salmonella Pathogenicity Island 1 Expression in Response to the Changes of Osmolarity

  • LIM, SANG-YONG;YONG, KYEONG-HWA;RYU, SANG-RYEOL
    • Journal of Microbiology and Biotechnology
    • /
    • v.15 no.1
    • /
    • pp.175-182
    • /
    • 2005
  • Abstract Salmonella pathogenicity island 1 (SPI1) gene expression is regulated by many environmental signals such as oxygen, osmolarity, and pH. Here, we examined changes in the expression level of various regulatory proteins encoded within SPI1 in response to three different concentrations of NaCl, using primer extension analysis. Transcription of all the regulatory genes tested was activated most when Salmonella were grown in Luria Broth (LB) containing 0.17 M NaCl. The expression of hilA, invF, and hilD was decreased in the presence of 0.47 M NaCl or in the absence of NaCl, while hilC expression was almost constant regardless of the NaCl concentration when Salmonella were grown to exponential phase under low-oxygen condition. The reduced expression of hilA, invF, and hilD resulted in lower invasion of hilC mutant to the cultured animal cells when the mutant was grown in the presence of 0.47 M NaCl or in the absence of NaCl prior to infection. Among the proteins secreted via the SPI1-type III secretion system (TTSS), the level of sopE2 expression was not influenced by medium osmolarity. Various effects of osmolarity on virulence gene regulation observed in this study is one example of multiple regulatory pathways used by Salmonella to cause infection.

Investigation on antimicrobial resistance genes of Salmonella Schwarzengrund isolated from pigs (돼지유래 Salmonella Schwarzengrund의 약제내성 유전자에 관한 연구)

  • Lee, Woo-Won;Kim, Sang-Hyun;Lee, Seung-Mi;Lee, Gang-Rok;Lee, Gi-Heun;Kim, Yong-Hwan
    • Korean Journal of Veterinary Service
    • /
    • v.35 no.1
    • /
    • pp.1-8
    • /
    • 2012
  • To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (${\beta}$-lactamase $bla_{TEM}$), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.

Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp. (Salmonella spp. 특이적인 검출을 위한 SYBR Green real-time PCR 기법 적용)

  • Shin, Seung Won;Cha, Seung Bin;Lee, Won-Jung;Shin, Min-Kyoung;Jung, Myunghwan;Yoo, Anna;Jung, Byeng Yeal;Yoo, Han Sang
    • Korean Journal of Veterinary Research
    • /
    • v.53 no.1
    • /
    • pp.25-28
    • /
    • 2013
  • The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle ($C_T$) of Salmonella spp. was $11.83{\pm}0.78$ while non-Salmonella spp. was $30.86{\pm}1.19$. Correlation coefficients of standard curves constructed using $C_T$ versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity ($R^2=0.993$; slope = 3.563). Minimum level of detection with the method was > $10^2$ colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.

A Design of AES-based Key Wrap/Unwrap Core for WiBro Security (와이브로 보안용 AES기반의 Key Wrap/Unwrap 코어 설계)

  • Kim, Jong-Hwan;Jeon, Heung-Woo;Shin, Kyung-Wook
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.11 no.7
    • /
    • pp.1332-1340
    • /
    • 2007
  • This paper describes an efficient hardware design of key wrap/unwrap algorithm for security layer of WiBro system. The key wrap/unwrap core (WB_KeyWuW) is based on AES (Advanced Encryption Standard) algorithm, and performs encryption/decryption of 128bit TEK (Traffic Encryption Key) with 128bit KEK (Key Encryption Key). In order to achieve m area-efficient implementation, two design techniques are considered; First, round transformation block within AES core is designed using a shared structure for encryption/decryption. Secondly, SubByte/InvSubByte blocks that require the largest hardware in AES core are implemented by using field transformation technique. As a result, the gate count of the WB_KeyWuW core is reduced by about 25% compared with conventional LUT (Lookup Table)-based design. The WB_KeyWuW con designed in Verilog-HDL has about 14,300 gates, and the estimated throughput is about $16{\sim}22-Mbps$ at 100-MHz@3.3V, thus the designed core can be used as an IP for the hardware design of WiBro security system.