Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Investigation on antimicrobial resistance genes of Salmonella Schwarzengrund isolated from pigs

돼지유래 Salmonella Schwarzengrund의 약제내성 유전자에 관한 연구

  • Lee, Woo-Won (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Kim, Sang-Hyun (Korea Research Institute of Bioscience & Biotechnology) ;
  • Lee, Seung-Mi (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Lee, Gang-Rok (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Lee, Gi-Heun (Veterinary Service Laboratory, Busan Metropolitan City Institute of Health and Environment) ;
  • Kim, Yong-Hwan (College of Veterinary Medicine, Gyeongsang National University)
  • 이우원 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 김상현 (한국생명공학연구원) ;
  • 이승미 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 이강록 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 이기흔 (부산광역시 보건환경연구원 축산물위생검사소) ;
  • 김용환 (경상대학교 수의과대학)
  • Received : 2012.02.15
  • Accepted : 2012.03.12
  • Published : 2012.03.30
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Abstract

To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (${\beta}$-lactamase $bla_{TEM}$), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.

Keywords

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