• 제목/요약/키워드: inv

검색결과 90건 처리시간 0.022초

약수에서 분리한 Yersinia pseudotuberculosis의 병원성과 16S rDNA 분석에 의한 분자학적 분류 (Molecular Taxonomy based on 16S rDNA Analysis and Pathogenicity of Yersinia pseudotuberculosis Isolated from Spring Waters)

  • 이영기;최성민;오수경;이강문;염곤
    • 미생물학회지
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    • 제37권1호
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    • pp.9-14
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    • 2001
  • 서울시내 25개 자치구에 산재한 약수터에서 5주의 Yersinia pseudotuberculosis를 분리하여 생화학적 특성, 병원성의 유무 및 16S rDNA 분석을 실시하였다. 분리된 Y. pseudotuberculosis는 모두 병원성 유전자인 inv를 소유하고 있었으며, 16S rDNA를 증폭한 후 염기서열을 분석하여 NCBI Genbank에 등록된 다른 Yersinia 속 및 장내세균 등과 비교해 본 결과 Yersinia 속 등과는 97.5%에서 100%의 높은 상동성(Similarity)을 나타내었고, 다른 장내세균 등과는 93.0%에서 95.1%의 낮은 상동성을 나타내었다. 165 rDNA 염기서열을 기초로 계통수를 작성한 결과 크게 3개의 cluster를 형성하였는데 특히 Y.enterocolitica (Z49830)은 Y.pseudotuberculasis (Z21939)와의 상동성(97.7%)보다 Y.intermedia (X75279)와의 상동성(97.9%)이 더 높게 나타났으며, E. coli (Z83205)는 Proteus vulgaris (AJ233425) 와의 상동성(93.2%)보다 Salmonella enteritidis (U90318)와의 상동성(97.7%)이 더 밀접한 연관성을 나타내었다.

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ON THE SPECTRAL RADIUS AND INVERTIBILITY OF CERTAIN ELEMENTS IN BANACH ALGEBRA

  • Park, Kyon-Hong;Kim, Byung-Do
    • Journal of applied mathematics & informatics
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    • 제4권1호
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    • pp.299-308
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    • 1997
  • In this paper we show that the limit of a convergent in-vertible sequence in the set of invertible elements Inv(A) in a Banach algebra A under a certain conditions is invertible and we investigate some properties of the spectral radius of banach algebra with unit.

A Prenatal Case of Paracentric Inversion of Chromosome 18, inv(18)(q21.1q22)

  • An, Gye-Hyeong;Kim, Moon Young;Kim, Min Hyoung;Kim, Yun Young;Choi, Kyu Hong;Kwak, Dong Wook;Park, So Yeon;Lee, Bom Yi;Park, Ju Yeon;Ryu, Hyun Mee
    • Journal of Genetic Medicine
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    • 제9권2호
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    • pp.101-103
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    • 2012
  • Paracentric inversion of chromosome 18 is a rare cytogenetic abnormality. The vast majority of paracentric inversions are harmless and the offspring of paracentric inversion carriers have only slightly elevated risks for unbalanced karyotypes. However, various clinical phenotypes are seen due to breakpoint variation or recombination. We report a prenatally detected case of familial paracentric inversion of chromosome 18, inv(18)(q21.1q22), with normal clinical features.

한반도의 소규모지진 모멘트 텐서 역산의 응용 (Application of Moment Tensor Inversion to Small Local Earthquakes in the Korean Peninsula)

  • 김소구;판티김반;이승규
    • 한국방재학회 논문집
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    • 제1권3호
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    • pp.123-136
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    • 2001
  • 모멘트 텐서 역산의 응용 목적은 지진 메커니즘, 지진 모멘트, 한반도에서 국지적으로 발생하는 규모 5.5 이하의 소규모 지진의 진원깊이 등의 진원인자를 결정하는 것이다. 이와 같이 광범위한 지형에서 발생하는 지진들은 어느 지역에서의 활동적인 지체구조를 밝혀내는 실마리를 제공해 준다. 더욱이 소규모 지진의 지진 메커니즘 연구를 위해서는 반드시 필요하다. 본 논문에서는 한반도에서 최근에 발생한 규모 3.3 - 4.8의 14개 지진을 모멘트 역산방법 (TDMT_INV)을 이용하여 지진 메커니즘 분석 결과를 소개하고 있다. 단층들의 주향은 북동-남서, 북서-남동 방향으로 주향이동 또는 소규모 역단층의 주향이동 단층을 보이고 있다. 응력장의 압축방향은 몇몇 단층을 제외하고는 동-서 또는 동북동-서남서 방향이 우세한 것으로 나타났다. 그리고 영남 육괴와 원산 지역에 분포하는 지진들은 남-북 또는 북북서-남남동 방향이 우세한 것으로 나타났다.

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오심, 구토, 구역질 측정도구의 신뢰도와 타당도 검증 (The Index of Nausea, Vomiting, and Retching(Korean Translation))

  • 김영재;김지영;최인령;김미원
    • 성인간호학회지
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    • 제12권2호
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    • pp.278-285
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    • 2000
  • Nausea, vomiting and retching are universal symptoms that affect individuals' state of health and self-care activities of individuals. Accurate measurements of individual symptoms are required to gather more definitive data, and enhance understanding, planning, and implementation of self-care actions. Recently the Rhodes Index of Nausea, Vomiting, and Retching(INVR), a new format of the INV-2(the Rhodes Index of Nausea and Vomiting), was developed to measure the symptoms of nausea, vomiting and retching in an English speaking population. To determine the reliability and validity of the INVR, and the possibility of using the instrument in Korea, the Korean translation of the INVR and the INV-2 were administered to a convenient sample of 105 patients at two University Hospitals in Kwangju, Korea. The Cronbach's alpha to estimate the internal consistency of reliability for INVR was 0.844. Equivalent measures of reliability were conducted to determine the percentage of agreement and the Spearman rank correlation coefficients for responses on the two instruments. The percent agreement was 83% and the correlation coefficient was 0.906 over all. A significant differences between the INVR scores of the patients with and without nausea, vomiting, or retching were seen, which indicated a construct validity. The INVR was found to be more user friendly for the patient and the healthcare providers. As a result, it is suggested that the INVR can provide a scientific base for measuring the symptoms of nausea, vomiting, and retching for nurses to improve patients' care and quality of life.

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3번 염색체 단완 결실과 장완 중복을 동반한 1례 (A Case of Short Arm Deletion and Long Arm Duplication at Chromosome 3)

  • 공승현;서정일;강장희;정소영;목지선
    • Clinical and Experimental Pediatrics
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    • 제48권12호
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    • pp.1389-1389
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    • 2005
  • 저자들은 출생 시 납작한 후두골, 낮은 변형 귀, 양안 격리증, 넓고 낮은 콧등, 얇은 입술, 넓고 짧은 목의 덧살, 저긴장증, 피부의 다모증, 잠복고환 등의 소견을 보이는 미숙아의 염색체 핵형 분석에서 부모의 불균형 전도로부터 재조합된 염색체 이상의 결과로 인해 46,XY,rec(3)dup(3)(q21)del(3)(p25)inv(3)(p25q21)로 진단된 증례를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

Analysis of Salmonella Pathogenicity Island 1 Expression in Response to the Changes of Osmolarity

  • LIM, SANG-YONG;YONG, KYEONG-HWA;RYU, SANG-RYEOL
    • Journal of Microbiology and Biotechnology
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    • 제15권1호
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    • pp.175-182
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    • 2005
  • Abstract Salmonella pathogenicity island 1 (SPI1) gene expression is regulated by many environmental signals such as oxygen, osmolarity, and pH. Here, we examined changes in the expression level of various regulatory proteins encoded within SPI1 in response to three different concentrations of NaCl, using primer extension analysis. Transcription of all the regulatory genes tested was activated most when Salmonella were grown in Luria Broth (LB) containing 0.17 M NaCl. The expression of hilA, invF, and hilD was decreased in the presence of 0.47 M NaCl or in the absence of NaCl, while hilC expression was almost constant regardless of the NaCl concentration when Salmonella were grown to exponential phase under low-oxygen condition. The reduced expression of hilA, invF, and hilD resulted in lower invasion of hilC mutant to the cultured animal cells when the mutant was grown in the presence of 0.47 M NaCl or in the absence of NaCl prior to infection. Among the proteins secreted via the SPI1-type III secretion system (TTSS), the level of sopE2 expression was not influenced by medium osmolarity. Various effects of osmolarity on virulence gene regulation observed in this study is one example of multiple regulatory pathways used by Salmonella to cause infection.

돼지유래 Salmonella Schwarzengrund의 약제내성 유전자에 관한 연구 (Investigation on antimicrobial resistance genes of Salmonella Schwarzengrund isolated from pigs)

  • 이우원;김상현;이승미;이강록;이기흔;김용환
    • 한국동물위생학회지
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    • 제35권1호
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    • pp.1-8
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    • 2012
  • To detect the virulence genes (invA and spvC) and antimicrobial resistance genes, polymerase chain reaction (PCR) was carried out using total 67 strains of S. Schwarzengrund isolated from pigs. As results, invA was detected from all 67 strains of S. Schwarzengrund, however, spvC was not at all. All 12 strains with ampicillin resistance, 15 strains with chloramphenicol resistance, 9 strains with kanamycin resistance, 1 strain with sulfamethoxazole/trimethoprim resistance, and 66 (98.5%) of 67 strains with tetracycline resistance carried TEM (${\beta}$-lactamase $bla_{TEM}$), cmlA (nonenzymatic chloramphenicol resistance), aphA1-Iab (aminoglycoside phosphotransferase), sulII (dihydropteroate synthase), and tetA (class A tetracycline resistance), respectively. All 63 strains with streptomycin resistance carried 3 aminoglycoside resistance genes, including aadA (aminoglycoside adenyltransferase), strA, and strB (streptomycin phosphotransferase). With respect to prevalence of antibiotic resistance genes occurred in S. Schwarzengrund, genes for strB (46.0%); strA and strB (30.2%); aadA, strA, and strB (9.5%); strA (7.9%); aadA and strB (3.2%); and aadA (3.2%) were detected by PCR.

Salmonella spp. 특이적인 검출을 위한 SYBR Green real-time PCR 기법 적용 (Application of SYBR Green real-time PCR assay for the specific detection of Salmonella spp.)

  • 신승원;차승빈;이원정;신민경;정명환;유안나;정병열;유한상
    • 대한수의학회지
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    • 제53권1호
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    • pp.25-28
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    • 2013
  • The aim of this study was to applicate and evaluate a SYBR Green real-time PCR for the specific detection of Salmonella spp. Specificity of the PCR method was confirmed with 48 Salmonella spp. and 5 non-Salmonella strains using invA gene primer. The average threshold cycle ($C_T$) of Salmonella spp. was $11.83{\pm}0.78$ while non-Salmonella spp. was $30.86{\pm}1.19$. Correlation coefficients of standard curves constructed using $C_T$ versus copy number of Salmonella Enteritidis ATCC 13076 showed good linearity ($R^2=0.993$; slope = 3.563). Minimum level of detection with the method was > $10^2$ colony forming units (CFU)/mL. These results suggested that the SYBR Green real-time PCR might be applicable for the specific detection of Salmonella spp. isolates.

와이브로 보안용 AES기반의 Key Wrap/Unwrap 코어 설계 (A Design of AES-based Key Wrap/Unwrap Core for WiBro Security)

  • 김종환;전흥우;신경욱
    • 한국정보통신학회논문지
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    • 제11권7호
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    • pp.1332-1340
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    • 2007
  • 본 논문에서는 휴대인터넷 와이브로 (WiBro) 시스템의 보안계층 중 암호 키 (Traffic Encryption Key; TEK)를 암호 복호하는 key wrap/unwrap 알고리듬의 효율적인 하드웨어 설계에 대해 기술한다. 설계된 key wrap/unwrap 코어 (WB_KeyWuW)는 AES (Advanced Encryption Standard) 알고리듬을 기반으로 하고 있으며, 128비트의 TEK를 128비트의 KEK (Key Encryption Key)로 암호화하여 192비트의 암호화된 키를 생성하고, 192비트의 암호화된 키를 복호화하여 128비트의 TEK로 복호하는 기능을 수행한다. 효율적인 하드웨어 구현을 위해 라운드 변환 블록에 하드웨어 공유기법을 적용하여 설계하였으며, 또한 하드웨어 복잡도에 가장 큰 영향을 미치는 SubByte/InvSubByte 블록을 체 변환 방법을 적용하여 구현하였다. 이를 통해, LUT (Lookup Table)로 구현하는 방식에 비해 약 25%의 게이트 수를 감소시켰다. Verilog-HDL로 설계된 WB_KeyWuW 코어는 약 14,300개의 게이트로 구현되었으며, 100-MHz@3.3-V의 클록으로 동작하여 $16{\sim}22-Mbps$의 성능이 예상되어 와이브로 시스템 보안용 하드웨어 구현을 위한 IP로 사용될 수 있다.