• 제목/요약/키워드: intraspecific variation

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RAPD를 이용한 고추냉이의 유연관계 분석 (Intraspecific Genetic Relation of Wasabia japonica Matsum. Based on RAPD Analysis)

  • 허수정;권순배;변학수;서정식;유기억
    • 한국약용작물학회지
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    • 제12권1호
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    • pp.31-35
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    • 2004
  • 국내에서 육성 또는 외국에서 도입되어 재배되고 있는 고추냉이 (Wasabia japonica Matsum.) 7품종의 10개체와 울릉도 자생 1개체, 총 11개체에 대한 유연관계 분석을 위하여 RAPD분석을 실시하였다. RAPD분석에 사용한 random primer는 총 50종류였으며 screen 결과 21종류만이 11품종 전체에서 반응을 보였다. 21종류의 primer로부터 관찰된 밴드는 총 144개였으며 이중 다형화 (polymorphism)를 보이는 앤드는 68개 (47.2%)로 primer 한 개당 평균 3.2개의 다형화 밴드를 보였다. Primer별 밴드의 수는 $2{\sim}13$개로 다양하게 나타났고 평균 밴드의 수는 6.8개였다. 유집분석 결과 phenogram은 유사도지수 $0.81{\sim}0.96$의 높은 범위 내에서 11개체들이 단계통군으로 유집되었으나 품종내 개체간에는 뚜렷하게 유집되지 않았다. 울릉도에 자생하는 개체의 경우는Sayatori와 Himenisiki 품종을 제외한 나머지 8개체를 위한 자매군으로 유집되었다. 이러한 결과는 neighborjoining 분석에서도 같은 경향을 보였다. 따라서 고추냉이의 종내 유연관계 분석을 위한 방법으로 RAPD법은 유용하지 않은 것으로 나타났으며 좀더 정확한 유연관계분석을 위해서는 AFLP방법이나 계통분류에 널리 이용되는 핵, 업록체 DNA의 유전자 염기서열 비교와 같은 정밀한 분자계통학적 연구가 수행되어져야 할 것으로 사료된다.

Phylogenetics of Trichaptum Based on Mitochondrial Small Subunit rDNA Sequences

  • Ko, Kwan-Soo;Jung, Hack-Sung
    • Journal of Microbiology
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    • 제35권4호
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    • pp.259-263
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    • 1997
  • To study the phylogenetic relationships of species of Trichaptum and to infer intraspecific dibergence of T. abietinum, partial mitochondrial small subunit rDNA sequences were determined. Six strains of T. abietinum, two of T. biforme, and one of T. fusco-violaceum were examined. Parsimony and distance analyses showed that each Trichaptum species forms a distinct group and that T. abietinum consists of two or more subgroups. Strains from North America were distantly related to one another but the European strain formed an independent group with three Korean strains, suggesting the possibility that Korean taxa may be phylogenetically closer to European taxa than to North American taxa.

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Comparative Genetic Diversity in Natural and Hatchery Populations of Indian Major Carps (C. catla and L. rohita)

  • Rana, R.S.;Bhat, K.V.;Lakhanpal, S.;Lakra, W.S.
    • Asian-Australasian Journal of Animal Sciences
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    • 제17권9호
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    • pp.1197-1203
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    • 2004
  • This study deals with the characterization of three populations (two hatchery and one natural) of Indian major carps Catla catla and Labeo rohita from different locations in India. The genetics of Indian major carps has been completely obscure and this is the first report on comparative allozyme variations in natural and hatchery population. The total 10 biochemical genetic markers used to measure interspecific and intraspecific level of diversity. The allele frequency data indicate different level of genetic variability in three populations. The hatchery population exhibited least polymorphism, low level of heterozygosity and genetic diversity.

First Record of Ulva torta (Ulvales, Chlorophyta) in Korea

  • An, Jae Woo;Nam, Ki Wan
    • 환경생물
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    • 제35권3호
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    • pp.329-334
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    • 2017
  • A marine ulvalean species (Chlorophyta) was collected from Imgok, which is located on the eastern coast of Korea. This species is morphologically characterized by distromatic, filiform to strap compressed or tubular thallus. Many branches were found near the base, but lacked proliferations. Cells were longitudinally aligned in the younger part of the branch and were disordered in the older part of the branch. A cap-like parietal chloroplast with one or two pyrenoids was contained in each cell. In a phylogenetic tree based on ITS and rbcL sequences, this species was nested in the same clade as Ulva torta and U. clathratioides from Australia, but formed a sister clade to U. torta from Japan. However, the genetic divergence between them was included in an intraspecific variation range within Ulva. This finding suggests that U. clathratioides should be reduced to a synonym of U. torta. Accordingly, the Korean alga was identified as U. torta based on the morphological and molecular data. This investigation is the first record of U. torta in the Korean marine algal flora.

First Record of Eucephalobus oxyuroides (Nematoda: Rhabditida: Cephalobidae) from South Korea

  • Kim, Taeho;Bae, Yeon Jae;Park, Joong-Ki
    • Animal Systematics, Evolution and Diversity
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    • 제33권4호
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    • pp.256-261
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    • 2017
  • Eucephalobus oxyuroides (de Man, 1876) Steiner, 1936 belonging to the family Cephalobidae Filpijev, 1934 (Cephalobomorpha) is newly reported from South Korea. This species is distinguished from other Eucephalobus species by its pointed tail terminus, high lips and relatively longer female tail and body. From many previous studies, intraspecific variation in this species has been reported for body length, absence/presence of probolae, and number/position of papillae in the posterior region of male, posing an obstacle for identification and phylogenetic analysis. In this study, details of the morphological characters and morphometric of E. oxyuroides Korean population are described and illustrated based on optical microscopy.

Hemiculter leucisculus의 동종이명 (Hemiculter eigenmanni (Jordan and Metz, 1913), a Junior Synonym of H. leucisculus (Basilewsky, 1855) (Cypriniformes: Cyprinidae))

  • 조현근;김병직;최윤
    • 한국어류학회지
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    • 제24권4호
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    • pp.287-291
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    • 2012
  • Hemiculter eigenmanni (Jordan and Metz, 1913)의 유효성을 알아보기 위하여, Hemiculter leucisculus (Basilewsky, 1855)의 총모식 표본 2개체, H. eigenmanni (Jordan and Metz, 1913)의 완모식 표본에 대해 형태학적 분석을 수행하였다. 연구 결과, 이들 2종의 일부 형태학적 차이점들은 한국에서 채집된 다수의 비교 표본들을 통하여 종내의 변이임이 밝혀졌고, H. eigenmanni는 H. leucisculus의 신참 동종이명으로 확인되었다.

Ecotypic Variation in Salinity Responses of Ulva pertusa(Chlorophyta) from the Korean Coast

  • Kim, Kwang-Young;Suh, Hae-Lip
    • Journal of the korean society of oceanography
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    • 제31권1호
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    • pp.1-6
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    • 1996
  • Salinity ecotypes in Ulva pertusa Kjellman were examined for the growth responses of the three isolates taken from different salinity regimes. All isolates showed a broad salinity tolerance, but growth patterns were correlated with the salinity regime of their original habitat. The germlings from Anin exhibited optimum growth at the native salinity of 32%. The germlings from Yongyon which had hypersaline habitats were tolerable to high salinity, i.e. growth rates peaked at 40%, whereas those from Samgando which had low salinities achieved maximum growth rate at 24\%. The germlings of inter-isolate cross demonstrated intermediate growth response between that of their respective parents. Our data also clearly indicated intraspecific differences among the three isolates, which was interpreted as development of different physiological ecotypes. We conclude that U. pertusa may consist of several ecotypes, each of which has some capacity for physiological adaptation to salinity variations.

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Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis

  • Lee, Soo-Ung;Huh, Sun
    • Parasites, Hosts and Diseases
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    • 제42권3호
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    • pp.145-148
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    • 2004
  • We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang), The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.

미국내 바랭이의 지역적 분포와 잡초로서의 중요성 (Geographical Distribution and Importance of Crabgrass (Digitaria spp.) in the United States)

  • 김태준
    • 아시안잔디학회지
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    • 제12권2호
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    • pp.99-106
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    • 1998
  • 미국내에서 바랭이의 지역적 분포와 각 재배작물에서 잡초로서의 중요성을 자료화하기 위하여 설문조사를 실시하였다. 총 117개의 배포된 설문지중 72개의 설문지가 회수되어 62%의 응답률을 보였다. 미극내의 농, 원예작물 재배지역에는 5종의 바랭이가 분포하는 것으로 보고되었고, 이들은 large, smooth, southern, india 그리고 blanket crabgrass 등이었다. 이들 중 약 50% 이상의 응답자가 large crabgrass를 가장 문제잡초로 인식하고 있었다. 지역적으로 smoth와 large crabgrass는 모든 위도에서 그 우점도가 인정된 반면, southern crabgrass는 우점도가 남부지역에 제한되는 것으로 보고되었다. 바랭이는 다른 작물의 재배지역보다 잔디밭에서 그 문제가 보다 심각한 것으로 보고되었다. 남부지역의 잔디밭에서는 large, southern 그리고 smooth crabgrass의 순서로, 북부지역에서는 smooth crabgrass가 large crabgrass보다 잡초로서의 문제성이 큰 것으로 보고되었다. 대부분의 응답자들은 종내변이성이 smooth와 large crabgrass에는 존재하지 않는 것으로 응답하였으나, southern crabgrass내에는 존재하는 것으로 보고되었다.

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The complete chloroplast genome of Limonium tetragonum (Plumbaginaceae) isolated in Korea

  • KIM, Yongsung;XI, Hong;PARK, Jongsun
    • 식물분류학회지
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    • 제51권3호
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    • pp.337-344
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    • 2021
  • The chloroplast genome of Limonium tetragonum (Thunb.) Bullock, a halophytic species, was sequenced to understand genetic differences based on its geographical distribution. The cp genome of L. tetragonum was 154,689 bp long (GC ratio is 37.0%) and has four subregions: 84,572 bp of large single-copy (35.3%) and 12,813 bp of small single-copy (31.5%) regions were separated by 28,562 bp of inverted repeat (40.9%) regions. It contained 128 genes (83 protein-coding genes, eight rRNAs, and 37 tRNAs). Thirty-five single-nucleotide polymorphisms and 33 INDEL regions (88 bp in length) were identified. Maximum-likelihood and Bayesian inference phylogenetic trees showed that L. tetragonum formed a sister group with L. aureum, which is incongruent with certain previous studies, including a phylogenetic analysis.