Parasites, Hosts and Diseases

The Korea Society for Parasitology and Tropical Medicine (대한기생충학·열대의학회)
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Variation of nuclear and mitochondrial DNAs in Korean and Chinese isolates of Clonorchis sinensis

  • Lee, Soo-Ung (Department of Parasitology, College of Medicine, Hallym University) ;
  • Huh, Sun (Department of Parasitology, College of Medicine, Hallym University)
  • Published : 2004.09.01
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Abstract

We compared the DNA sequence difference of isolates of Clonorchis sinensis from one Korean (Kimhae) and two Chinese areas (Guangxi and Shenyang), The sequences of nuclear rDNA (18S, internal transcribed spacer 1 and 2: ITS1 and ITS2) and mitochondrial DNA (cytochrome c oxidase subunit 1: cox1) were compared. A very few intraspecific nucleotide substitution of the 18S, ITS1, ITS2 and cox1 was found among three isolates of C. sinensis and a few nucleotide insertion and deletion of ITS1 were detected. The 18S, ITS1, ITS2 and cox1 sequences were highly conserved among three isolates. These findings indicated that the Korean and two Chinese isolates are similar at the DNA sequence level.

Keywords

References

  1. Ando K, Sithithaworn P, Nuchjunggreed C, et al. (2001) Nucleotide sequence of mitochondrial COI and ribosomal ITSII of Opisthorchis viverrini in Northeast Thailand. Southeast Asian J Trop Med Public Health 32: 17-22
  2. Barker SC, Blair D (1996) Molecular phylogeny of Schistosoma species supports traditional groupings within the genus. J Parasitol 82: 292-298 https://doi.org/10.2307/3284163
  3. Bowles J, Blair D, McManus DP (1992) Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol 54: 165-173 https://doi.org/10.1016/0166-6851(92)90109-W
  4. Bowles J, Blair D, McManus DP (1995) A molecular phylogeny of the human schistosomes. Mol Phylot Evol 4: 103-109 https://doi.org/10.1006/mpev.1995.1011
  5. Bowles J, Hope M, Tiu WU, Liu X, McManus DP (1993) Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum. Acta Trop 55: 217-229 https://doi.org/10.1016/0001-706X(93)90079-Q
  6. Higgins DG, Bleasby AJ, Fuchs R (1994) CLUSTAL W: Improved software for multiple sequence alignment. Comput Appl Biosci 8: 189-191
  7. Morgan JA, Blair D (1998) Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial COI and ND1 genes for distinguishing among Echinostoma species (Trematoda). Parasitology 116: 289-297 https://doi.org/10.1017/S0031182097002217
  8. Park GM, Yong TS (2001) Geographical variation of the liver fluke, Clonorchis sinensis, from Korea and China based on the karyotypes, zymodeme and DNA sequences. Southeast Asian J Trop Med Public Health 32(Suppl 2): 12-16
  9. Rim HJ (1986) The current pathobiology and chemotherapy of Clonorchiasis. Korean J Parasitol 24(suppl): 5-141
  10. Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463-5467 https://doi.org/10.1073/pnas.74.12.5463
  11. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd ed. Chapter 6, pp 6.1-6.30. Cold Spring Harbor Laboratory Press, New York, USA