• 대한임상검사과학회지
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• 제36권2호
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• pp.173-177
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• 2004

This study is performed in order to clarify the histochemical structure, the distribution of mucous cell and goblet cell, and the histochemical properties of the mucosubstances in the middle region of intestinal mucosa and rectum of Raja kenojei. In the H&E stain, distribution of the mucous cells and acidophilic cells were a more compacted middle portion than other regions of intestine, but the former was more than the latter in the number of mucous cells to rectum. The mucosubstances were stained with aldehyde fuchsin pH 1.7-alcian blue(pH 2.5) stain and then compared to distribution of the mucosubstances used in image and microscope technology(IMT-Size5). The middle intestine of Raja kenojei was composed of mucous cells having only large amounts of mucosubstances in the distal region was much more than that of proximal region. It was two types of mucous cells to rectum, one type was the same as proximal intestine while the other had small amounts of weakly sulfated and large amounts of carboxylated mucins.

• 대한한의학원전학회지
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• 제23권2호
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• pp.225-233
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• 2010

Conceptions about functions of large & small intestine[LI & SI] were focused on the vermiculation in "Somun(素問) Yeongranbijeonron(靈蘭秘典論)". However, functions of large & small intestine includes more. In Oriental Medicine, there are sentences in "Hwangjenaegyeong(黃帝內經)" "LI manages Fluid [津] and SI manages Humor[液]" It means that LI & SI have an each role in digestion besides vermiculation. In that reason, we try to find out the meaning of the functions of LI and SI in digestion through bibliographic review. As a result, LI and SI have a digestic function by Separating the Clear which includes Fluids and Humor and the Turbid which is relatively useless to the Clear.

• 대한한방소아과학회지
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• 제1권1호
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• pp.23-37
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• 1986

Although the Insamyangwee-Tang has been widely used in clinical purposes in the oriental medicine and its clinical efficiency is documented for the cases of gastritis, gastric ulcer and enteritis but the experimental study on these has not been undertaken. So,To investigate the clinical efficiency, of Insamyangwee-Tang and validate its oriental medical theory, these experiments were undertaken, by being compared with animal experiment. ?The following results were obtained. ?1. By effect of Insamyangwee-Tang on isolated ileum from mice, rats, rabbits and Guinea pigs, the motility of ileums was inhibited remarkably. ?2. By effect of Insamyangwee-Tang on motility of the small intestine in rabbits, the motility was inhibited remarkably too. ?3. Insamyangwee-Tang inhibited charcoal transport functions in the small intestine of mice. ?4. By effect of Insamyangwee-Tang on isolated duodenum from rats, the motility of duodenum was inhibited remarkably.?5. Gastric juice and pepsin secretion was decreased, anti-ulceration effect was recognized.

• IMMUNE NETWORK
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• 제13권6호
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• pp.227-234
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• 2013

The intestinal immune system has an ability to distinguish between the microbiota and pathogenic bacteria, and then activate pro-inflammatory pathways against pathogens for host defense while remaining unresponsive to the microbiota and dietary antigens. In the intestine, abnormal activation of innate immunity causes development of several inflammatory disorders such as inflammatory bowel diseases (IBD). Thus, activity of innate immunity is finely regulated in the intestine. To date, multiple innate immune cells have been shown to maintain gut homeostasis by preventing inadequate adaptive immune responses in the murine intestine. Additionally, several innate immune subsets, which promote Th1 and Th17 responses and are implicated in the pathogenesis of IBD, have recently been identified in the human intestinal mucosa. The demonstration of both murine and human intestinal innate immune subsets contributing to regulation of adaptive immunity emphasizes the conserved innate immune functions across species and might promote development of the intestinal innate immunity-based clinical therapy.

• 한국동물위생학회지
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• 제29권4호
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• pp.497-501
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• 2006

Acute hemorrhagic enteritis was diagnosed in a seven-month-old male Shitzu dog dying of blood stained diarrhea and vomiting. Clinical findings were anorexia, dullness and sudden death after massive bloody diarrhea. At necropsy, main lesion was the hemorrhage in small intestine, mainly duodenum and jejunum. Microscopically, Gram positive long bacilli were massively detected on the mucose epithelial cells and necrotic debris of small intestine. Coagulative necrosis of epithelial cells and thrombosis of small intestine were also identified. However, there was no lesion of crypt epithelium. Mineral infiltration in both gastric mucosa and renal tubules was detected and proliferation of fibrous tissue was also shown in corticomedullary regions. In bacterial examination, C perfringens was isolated in anaerobic culture and it was confirmed to type A by multiplex PCR. Therefore, the dog was diagnosed as C perfringens type A associated enteritis with uremia.

• Animal cells and systems
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• 제6권4호
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• pp.323-326
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• 2002

One hundred and three random complementary DNA clones representing rainbow trout intestine were par1i811y sequenced as an approach to analyze the transcribed sequences of its genome. Of the sequences generated, 60.0% of the ESTs were represented by 40 known genes. Thirty-five clones of unknown gene products potentially represented 34 novel genes. The most Bbundantly represented messages were the 28S ribosomal protein (6.5%) and beta actin (5.8%). The genes involved in ribosome formation (18%) accounted for the major gene expression. Development of EST panels representing the genes expressed in a particular tissue will be useful in determining the role of these genes in normal function and in response to developmental, hormonal, environmental and physiological changes.

• 한국임상수의학회지
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• 제36권4호
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• pp.225-228
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• 2019

Inflammatory bowel disease and alimentary lymphoma are common gastrointestinal disorder in cats. More recently, ultrasonographic features associated with feline alimentary lymphoma has been recognized as a diffuse thickening of muscular layer of small intestine. We investigated correlation between thickening of muscular layer of small intestine and such disease. We found a significantly increased thickness of the muscular layer of small intestine in cats with lymphoma or IBD compared with healthy cats. When a muscularis to submucosa ratio > 1 and regional lymphadenopathy were found during ultrasonography on cats with gastrointestinal signs, full-thickness biopsy could be recommended to make sure presence confirmation of inflammatory bowel disease or alimentary lymphoma.

• Journal of Acupuncture Research
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• 제19권3호
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• pp.180-191
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• 2002

Objective : This study was performed to evaluate the effect of oriental medical treatment in whiplash injury patients which caused by traffic accident. Methods : The clinical study was carried out 37 cases of whiplash injury patients which had been admitted in Daejon university Cheon-an oriental hospital from June, 2001 to December, 2001. Results : l. In the distribution of sex distinction was female much more than male in the ratio of 3:2 and thirties was most in age distribution. 2. In situation of traffic accident, The most case was rear-ending(70.27%). 3. Except cervical pain or it's reffered pain, there were whole body symptoms as fallow, insomnia in 11cases(29.73%), night pain in 10 cases(27.03%), general body pain in 8cases(21.62), dizziness in 6cases(16.22%). 4. According to Meridian Muscle theory, a injured parts of patient were devided as follows. There were 22cases(59.46%) which have injury in Bladder, Small Intestine Meridian Muscle, 10cases(27.03%) in Gallblader, Triple Energizer Meridian Muscle, 5cases(13.51%) in Stomach, Large Intestine Meridian Muscle. 5. There were 6cases(16.22%) of the grade of complete recovery, 10cases(27.03%) of the grade of excellent, 12cases(32.43%) of the grade of improvement, 6cases(16.22%) of the grade of disimprovement and 3cases(8.11%) of the grade of poor. So 28cases(75.68%) were efficacious. 6. Generally Herb-meds that have efficacy of Geo-Eohyeol were most used(27cases, 72.97%) in early stage. Secondly Herb-med that have efficacy of Yiqi-sunqi were used(20case, 54,05%) in middle stage and Herb-med of Bo-Qiheol were used(l0cases, 27.03%) in latter term. The most used Herb-med was Hoisu-san, Oyaksungi-san(22cases, 54.96%). 7. In l5cases which have Aqua-Acupuncture treatment, There were 3cases of Complete recovery, 5cases of Excellent, 4cases of Improvement, 2cases of Disimprovement, 1cases of Poor. 8cases(53.33%) were above Excellent grade, so it is more efficatious Than simple treatment without Aqua-Acupuncture(36.37%). 8. The improvement of Bladder, Small Intestine Meridian Muscle was 77.27%, Gallblader, Triple Energizer Meridian Muscle was 70% and Stomach, Large Intestine Meridian Muscle was 80%. Additional Aqua-Acupuncture treatment improved to be effective in Bladder, Small Intestine Meridian Muscle(77.78%), in Gallblader, Triple Energizer Meridian Muscle(75%), in Stomach, Large Intestine Meridian Muscle(100%). Conclusions : In this study, Oriental treatment especially Aqua-Acupuncture was effective in whiplash injury patients which caused by traffic accident.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• 제14권2호
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• pp.171-180
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• 2011

목 적: 고지방식이를 섭취한 흰쥐에서 식이 유발인자인 NPY와 장내 운동 조절인자인 Cajal 간질세포의 변화를 관찰하고자 하였다. 대상 및 방법: 동일 연령의 Sprague-Dawley계 수컷 흰쥐를 임의로 대조군(7마리)과 고지방식이군(7마리)으로 분리한 후, 대조군에는 일반 흰쥐 사료를 충분히 섭취시켰으며, 고지방식이군에게는 60% kcal 지방이 포함된 사료(중앙실험동물센터)를 6주 동안 물과 함께 충분히 섭취하도록 하였다. 6주 후 시상하부 조직 절편에 rabbit polyclonal NPY (1 : 1000, Abcam)항체를 반응시키고, 소장의 조직 절편에 rabbit polyclonal c-kit (1 : 400, Neuromics)항체를 반응시켜서 면역조직화학 검사를 시행하였다. 결 과: 고지방식이군의 시상하부에서 NPY의 면역 반응성이 강하게 나타났으며, 특히 PVN에서 NPY의 면역 반응 세포수의 증가를 관찰할 수 있었다. 고지방식이군에서 소장의 Auerbach's plexus 부위의 Cajal 간질 세포의 면역 반응성이 떨어졌으며, 환상근과 종주근 내 Cajal 간질 세포 수도 현저히 감소하였다. 결 론: 고지방식이를 섭취한 흰쥐에서 시상하부 NPY 면역반응 신경세포수와 발현의 증가는 NPY의 저항성과 관련되며, 소장에서 Cajal 간질 세포의 감소는 장운동의 이상을 초래할 수 있음을 나타낸다.

• 한국콘텐츠학회논문지
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• 제10권7호
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• pp.297-304
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• 2010

소장 흡수상피 세포는 점액 다당류가 풍부하고 림프구와 사립체가 많아 방사선에너지에 민감하다. 따라서 세포 손상은 영양분 결핍 및 전해질 대사의 불균형을 가져와 장관사의 주 원인이 된다. 본 연구는 소장 흡수상피 세포들의 방사선 손상기전과 방어기작을 밝히고자 프로폴리스를 복강에 투여한 후 초 미세구조를 관찰하였다. 관찰 결과, 5Gy 조사 후 20일된 조직에서 소장이 유착되고, 표면이 검게 변해 있었으며 탄력성이 줄어든 것을 볼 수 있었다. 광학현미경(LM) 관찰 결과, 배상세포의 크기 및 수가 줄어들었으며 파네트과립세포는 위축되고 일부에서는 공포가 관찰되었다. 전자현미경(TEM) 관찰 결과, 미세융모와 용해소체는 정상적으로 관찰되었으나 사립체 막이 손상되고 림프구 가장자리에서 요철면이 형성되었다. 회장에서는 흡수상피조직이 비후되고 공포가 관찰되었다. 하지만 프로폴리스 복강 투여 후 관찰에서는 사립체의 이중막이 정상적으로 관찰되었고, 미세융모의 당질층도 손상되지 않았으며, M세포도 많이 관찰되어 자기소화와 흡수 기능이 원활하였다. 따라서 세균 바이러스 등의 면역기능과 세포 손상에 방어효과를 확인하였다.