• Journal of Microbiology and Biotechnology
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• v.12 no.4
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• pp.622-627
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• 2002

Diphtheria toxin repressor (DtxR) binds to approximately 30 to 35-bp regions containing an interrupted 9-bp inverted repeat within a 19-bp core sequence. The core sequence is fairly conserved and critical for DtxR binding. The flanking regions that are consisted of 5 to 8 more of nucleotides from the core are also required for DtxR binding. The nucleotides in both flanking regions are A-T rich. To examine whether the A-T nucleotides in both flanking regions from the core have significant roles for DtxR binding, a DNA fragment was constructed based on the diphtheria tox promoter/operator, and DNA fragments with substitution of A and T nucleotides In the flanking regions to G and C were also constructed. To assess the effect of these substitutions on binding of DtxR and repressibility by DtxR, $\beta$-galactosidase activity from lacZ fused to the region was assessed. Gel mobility shift of the region by purified DtxR was also examined. The DNA fragments containing the mutations in the flanking regions still exhibited repression and mobility shift with DtxR. The core segment with the mutation is still, therefore, recognized by DtxR. Nonetheless, the results from the assays indicated that the substitution significantly decreased repression of the operator by DtxR in vivo under high-iron condition and decreased binding of DtxR to the operator. These results suggest that A and T nucleotides fur both flanking regions are preferred for the binding of DtxR.

• Journal of Chest Surgery
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• v.17 no.1
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• pp.150-156
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• 1984

Paraesophageal hiatus hernia represents a rare hiatal hernia that are treated surgically. The completely asymptomatic paraesophageal hernia often does not reach the clinician or surgeon. But the presence of a symptomatic paraesophageal hernia is sufficient indication for surgery. The paraesophageal hernia may be approached either transthoracically or transabdominally. The general technique is essentially the same, whichever route is used. From either transthoracic or transabdominal approach, following reduction of the viscus and elimination of the sac, the diaphragmatic opening is then closed with interrupted heavy dacron or silk sutures in paraesophageal hiatus hernia. But if the phrenoesophageal membrane often is destroyed when the esophagogastric junction and the distal esophagus have been mobilized, it becomes important to fix the esophagogastric junction below the diaphragm, so that it does not slide through the hiatus and produce a sliding-type hiatus hernia in future. We have experienced one case of paraesophageal hiatus hernia which was accompanied with severe anemia in child. We preferred to approach through left thoracotomy incision and then pushed down the stomach into the abdominal cavity with complete excision of the hernial sac. We employed Belsey Mark V procedure using of Teflon felt pledgets with the mattress sutures against development of sliding-type hiatus hernia in postoperative period. postoperative course has been uneventful and good for about 3 months to this time.

• Journal of Chest Surgery
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• v.8 no.2
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• pp.119-124
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• 1975

This is a case report of the rupture of the trachea and the esophagus following external blunt trauma without any associated injury. A 7 year old male patient was brought to the emergency room. Hanyang University Hospital, on 23 Jul, 1974 while he played under the pile of pingpong table A table was fall down over the patient accidentally. The patient was injured by the table and found lying in unconsciousness on the ground.There was subcutaneous emphysema on his anterior chest and neck. A contusion on the upper part of the sternum was noted. The breathing sound were diminished over the left side of the chest. Ronchi were heard over the right lung field. This patient was suffered from vomiting, dypsnea, and irritable mental state after this accident. On the chest roentgenogram in A-P view, hyperlucency at the mediastinum and parapericardiac area suggested the pneumomediastinum. On the next day, a diagnosis of the tracheal and esophageal rupture was confirmed by the esophagogram with Lipiodol swallowing. A right thoracotomy was performed and ruptured orifice of the trachea and the esophagus were closed with interrupted sutures. Postoperative course was uneventful and primary closure of the rupture of the trachea and the esophagus were succeeded.

• Journal of Chest Surgery
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• v.25 no.4
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• pp.429-434
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• 1992

We experienced a rare case of traumatic ventricular septal defect by penetrating stab injury The patient was 26-year-old women who got stab wound at the left anterior third intercostal space and left sternal border with a knife. seven hours after admission, the patient was undertaken an emergency thoracotomy due to hypovolemic shock caused by massive bleeding from transected left internal mammary artery, vein, and right ventricular outflow tract. On postoperative second day, the patient was suffered from moderate dyspnea, and arterial blood gas analysis and chest X-ray revealed hypoxemia and pulmonary edema. Right heart cardiac catheterization with Swan-Ganz Cathater showed oxygen step-up between right atrium and main pulmonary artery and a 1.6:1 ratio of pulmonary to systemic blood flow. At operation, harsh systolic thrill was palpable along right ventricular outflow tract. Through small vertical right ventriculotomy, the linear ventricular septal laceration on infundibular septum was noticed, and its size was 1.5cm with sharp margin This defeat was repaired by three interrupted matress sutures using Prolene 4-O with pledget. Her postoperative course was uneventful, and she discharged with good physical condition.

• Journal of Microbiology
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• v.37 no.3
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• pp.141-147
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• 1999

Thirteen transcriptionally-fused carbon starvation mutants, derived from Pseudomonas putida ATCC 12633, were analyzed for their survivability and transcriptional induction profiles upon carbon starvation. One of these mutants, MK114, which exhibited the lowest survivability and the highest induction rate, was selected and further examined under different starvation (nitrogen and phosphate) and stress (osmolarity, H2O2, salts, alcohol, and heat) conditions. Under all tested conditions MK114 induced ${\beta}$-galactosidase activity, implying that the interrupted gene (cst114) is a general starvation and stress response gene. The rate of induction ranged from 2.6-fold for phosphate starvation to 3.7-fold for osmotic shock. The mini-Tn5 flanking DNA was cloned from the chromosome of MK114. The cloned DNA fragment exhibited carbon starvation activity, indicating that this fragment contains a carbon starvation-related promoter region. This region was partially sequenced. Possible physiological roles of Cst114 in a carbon sensing mechanism and in other stress responses are also discussed.

• Journal of the Korean Home Economics Association
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• v.47 no.2
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• pp.81-95
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• 2009

The purpose of this study is to examine gender equity in the quantity and flow experience of leisure time of urban dual-earner couples having preschool children. The quantity of leisure time is measured by primary activity and the flow experience of leisure time is measured by primary and secondary activities. The data collected by Korean National Statistical Office in 2004 is used. The sample for this study consists of 255 couples, and the statistical methods are frequency, percentage, paired t-test, and one-way ANOVA. The following is a summary of the major findings. The first, the pattern of the leisure time was different between husbands and wives. The quantity of leisure time of husbands was longer than that of wives. The husbands spent more time to perform social activities, media contact, sports and outdoor activities, while women did more in religious activities. The second, husband's leisure flow experience was higher than that of wives. The pure leisure time of wives was shorter than that of husbands and wives experienced more contaminated leisure time by a secondary activity. In other words, wives's leisure is more likely to be interrupted, to involve episodes of shorter duration, and to be associated with personal care and unpaid work.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.1536-1537
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• 2011

The high-voltage circuit breaker plays an important role in the electrical system because there has been a need for suitable switching devices capable of initiating and interrupting the flow of the electric fault current. It continues as the contacts recede from each other and as the newly created gap is bridged by a plasma. The arc plasma happens inside the insulation nozzle of SF6 self-blast interrupter which is newly developed as the next-generation switching principle. The ablation of PTFE nozzle is caused by this high temperature medium, the PTFE vapor from the nozzle surfaces flows toward the outlets and the pressure chamber. The vapor makes the pressure of the chamber increased by heat and mass transfer from the arcing zone. Because the rate of ablation depends on the magnitude of applied current, it decreases when the current goes to zero. The compressed gas inside the chamber flows reversely toward the arc plasma during this moment. According to this principle, the arc can be cooled down and the fault current can be interrupted successfully. In this study, we calculate arc plasmas and thermal-flow characteristics caused by fault current interruption inside a SF6 self-blast interrupter, and to investigate the effect of PTFE ablation on the whole arcing history.

• Journal of Chest Surgery
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• v.17 no.2
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• pp.244-249
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• 1984

Surgical treatment of partial endocardial cushion defect was accomplished in Feb. 1984 in this department. The 5 year old male patient had history of frequent upper respiratory tract infection and since his age of 3 years dyspnea on exertion and palpitation were noted but there were no cyanosis and clubbing. A thrill was palpable on the apex and grade IV/IV harsh systolic ejection murmur and diastolic murmur was audible on it. Liver was palpable about 3 finger breadths and no ascites. Chest X-ray revealed increased pulmonary vascularity, moderate cardiomegaly [C-T ratio; 0.69], and enlarged left atrium. EKG showed first degree heart block, RVH, LVH, and LAD. Echocardiogram showed paradoxical ventricular septal movement and abnormal diastolic movement of the anterior leaflet of mitral valve. Right heart catheterization resulted left to right shunt [Qp:Qs:2.1:1 ] and moderate pulmonary hypertension [60/40 mmHg]. Left ventriculogram showed mitral regurgitation [Grade III/IV] and filling of left atrium and right atrium nearly same time. Operative findings were: 1.Primum type atrial septal defect [3x2 cm] 2.Cleft on the anterior leaflet of mitral valve. 3.No interventricular communication and cleft of tricuspid valve leaflet. The mitral cleft was repaired with 4 interrupted sutures. The primum type atrial septal defect was closed with Dacron patch intermittently at endocardial cushion and continuously remainder. The post operative course was uneventful and discharged on 22nd postoperative day in good general conditions.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.2
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• pp.43-50
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• 2000

A CBN wheel was used for the highly efficient and precision grinding of the mold material(STD11). The grinding form accuracy by a CBN wheel is very excellent due to its low wheel wear, but grinding fragments resemble fine powders rather chips. A fine powders by this fragmentation can easily get attached to the wheel surface and therefore causing a loading. In order to prevent this fragmentation phenomena, the alumina stick is use to processing. Because the dressing with alumina stick should be interrupted for a processing, the automation of the processing and high productivity was very difficult. The investigation on the effect of Ultrasonic In-Process Dressing(ULID) on the grinding characteristics focuses in this Paper. This ULID method is that ultrasonic vibration in my Position of wheel is used to remove impurities on the wheel surface. Finally, the rate of surface roughness change in grinding by the ULID method was less than grinding without ultrasonic vibration. Loading phenomena by the ULID method were more prevented than grinding without ultrasonic vibration.

• Proceedings of the Korea Concrete Institute Conference
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• 2002.05a
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• pp.607-612
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• 2002

In road pavements, it is known that cement concrete pavement has superior durability, safety compared. But in repairing pavement, cement concrete pavement is not usually applied because of the length of time while the road is interrupted when using Ordinary and Rapid-hardening Portland Cement. And Super High Early Strength Cement and Ultra Super High Early Strength Cement are not favorable for ready mixed concrete because of rapid setting time, high slump loss and other restrictions. We developed special cement developing 1 day strength of over 30.0N/mm$^2$ to open the road within 1 day and workable time is maintained over 1 hour so that it can be used as ready mixed concrete. We performed experimental overlay construction with the cement and evaluated the mechanical property and the durability. At curing temperature of 8-l8$^{\circ}C$,the flexural strength was 6.4N/mm$^2$at 1 day, so that the road can be open to traffic within 1 day. In durability test, the hardened concrete showed higher durability than Portland cement concrete.