• BMB Reports
• /
• 제39권1호
• /
• pp.105-110
• /
• 2006

We have screened 766 strains of fungi from the BIOTEC Culture Collection (BCC) for xylanases working in extreme pH and/or high temperature conditions, the so-called extreme xylanases. From a total number of 32 strains producing extreme xylanases, the strain BCC7928, identified by using the internal transcribed spacer (ITS) sequence of rRNA to be a Marasmius sp., was chosen for further characterization because of its high xylanolytic activity at temperature as high as $90^{\circ}C$. The crude enzyme possessed high thermostability and pH stability. Purification of this xylanase was carried out using an anion exchanger followed by hydrophobic interaction chromatography, yielding the enzyme with >90% homogeneity. The molecular mass of the enzyme was approximately 40 kDa. The purified enzyme retained broad working pH range of 4-8 and optimal temperature of $90^{\circ}C$. When using xylan from birchwood as substrate, it exhibits $K_m$ and $V_{max}$ values of $2.6{\pm}0.6\;mg/ml$ and $428{\pm}26\;U/mg$, respectively. The enzyme rapidly hydrolysed xylans from birchwood, beechwood, and exhibited lower activity on xylan from wheatbran, or celluloses from carboxymethylcellulose and Avicel. The purified enzyme was highly stable at temperature ranges from 50 to $70^{\circ}C$. It retained 84% of its maximal activity after incubation in standard buffer containing 1% xylan substrate at $70^{\circ}C$ for 3 h. This thermostable xylanase should therefore be useful for several industrial applications, such as agricultural, food and biofuel.

• Journal of Advanced Marine Engineering and Technology
• /
• 제39권3호
• /
• pp.201-205
• /
• 2015

극지와 해양에서 파이프라인은 최저 약 $-40^{\circ}C$의 매우 낮은 극한 온도에 노출된다. 이 경우 내부 유체는 상온에서와는 다른 유동특성을 나타내며, 3차원 유동해석을 통해 극한온도에 의한 내부유체의 압력, 온도, 속도, 액적분포 등의 특성 변화를 분석해야 한다. 또한 영하의 온도로 인해 내부 유체의 액적이 응결되며, 이로 인해 곡관부에서 충돌침식이 발생할 것으로 예상된다. 이러한 충돌침식은 장기간 운용되는 자원 이송망 등의 파이프라인의 안정성에 영향을 준다. 본 논문에서는 다성분 다상 유동 해석을 통해 극한 온도조건의 극지 및 해양 파이프라인에서 내부유체의 유동특성 및 충돌 침식에 대한 분석을 수행하였다.

• 대한기계학회논문집B
• /
• 제35권7호
• /
• pp.693-699
• /
• 2011

바이오매스나 기타 유기성 폐기물로부터 가스화공정을 거쳐 얻을 수 있는 합성가스는 환경보호와 화석연료고갈 방지 측면에서 유망한 대체연료 중 하나로 여겨지고 있다. 그러나 가스화로부터 얻어지는 합성가스는 일반적인 천연가스와 같은 가스연료에 비해 발열량이 낮고 연료조성이 불균일하여 내연기관에 적용시 안정적이고 지속적인 운전이 어렵다는 단점이 있다. 따라서 본 연구에서는 이러한 저발열량과 불균일한 가스 조성의 특징을 가진 합성가스가 연소에 미치는 영향을 파악하여 고효율의 엔진을 개발하고자 연구를 수행하였다. 저발열량의 합성가스를 모사하기 위하여 천연가스에 질소를 희석한 연료를 사용하였다. 또한 체적당 발열량을 유지하면서 동일유량조건으로 합성가스에 수소 혼합비율을 10 ~ 30%로 변화시키면서 연소 특성 변화를 살펴보았다.

• 대한핵의학회지
• /
• 제22권2호
• /
• pp.193-197
• /
• 1988

Measurement of gastric emptying rate has been performed by a variety of techniques. Nuclear medicine method is a major advance in the quantitative evaluation of gastric function and also of pharmacologic intervention. Normal gastric emptying rate was measured in 48 healthy volunteers using live chicken liver labeled with $^{99m}Tc-Tin$ Colloid as a solid phase marker. In vitro studies were performed to evaluate the labeling efficiency and stability in hydrochloric acid and in human gastric juice of intracellularly labeled chicken liver. Anterior image counts only were compared with the geometric mean of anterior and posterior counts in 12 healthy volunteers who were studied by both anterior count and posterior count. The results were as follows: 1) The labeling efficiency in gastric juice and hydrochloric acid were $95.5{\pm}1.23%,\;95.7{\pm}1.15%$, respectively. 2) Half gastric emptying time by anterior count only was $126{\pm}21$ minutes 3) Although standard deviation of geometric mean method was smaller than anterior count method, gastric emptying curves from both method were similar. In daily practice, anterior count method may be useful alternative to geometric mean method in evaluation of gastric emptying rate.

• 대한물리의학회지
• /
• 제11권1호
• /
• pp.83-91
• /
• 2016

PURPOSE: There are several methods, such as the abdominal drawing-in maneuver (ADIM), maximal expiration (ME), and Kegel exercise, to strengthen the core muscles. However, to date no study has been conducted to compare the effects of the ADIM, ME, and Kegel exercise on the transverses abdominis (TrA), internal oblique (IO), external oblique (EO), and pelvic floor muscles (PFMs). The purpose of this study was to find out which of the three aforementioned exercises is most effective for contracting the core muscles. METHODS: The thickness of the TrA, IO, EO and PFMs was measured by ultrasonographic imaging during the ADIM, ME and Kegel exercise in 34 healthy participants. RESULTS: There was the significant difference between ADIM and Kegel exercise in the thickness of the TrA (p<0.05). There were the significant differences between ADIM and ME and between ME and Kegel exercise in the thickness of the IO and PFM (p<0.01). There was no significant activation in the thickness of the EO (p>0.05). Measurement reliability was assessed using intraclass correlation coefficients (ICC) and the standard error of measurement (SEM). An ICC value of >0.77 indicated that reliability measurements was good. CONCLUSION: Kegel exercise was the most effective exercise for the TrA and the PFM, and ME was the most effective exercise for the IO muscles.

• 대한물리의학회지
• /
• 제12권4호
• /
• pp.1-9
• /
• 2017

PURPOSE: This study investigated the effect that an elastic therapeutic taping treatment given to patients with chronic ankle instability had on the vertical ground reaction force, center of pressure, and range of motion in the ankle, knee and hip joints, during a Cross-cutting movement from landing. METHODS: This study analyzed 12 able-bodied adults and 12 patients with chronic ankle instability classified by using the Cumberland tool in the motion analysis laboratory, Hanseo University. The experiment was conducted under two conditions elastic taping and no treatment. In order to analyze the difference between the groups. An independent t-test was performed at p>.01. RESULTS: Plying an elastic therapeutic taping to the patients with chronic ankle instability significantly decreased the range of joint motion in the inversion of the ankle joint, the flexion of the knee joint, and the flexion and internal rotation of the hip joint during a cross-cutting movement from landing in comparison with the able-bodied adults p<.01. This restriction in the range of motion decreased the center-of-pressure trajectory length of patients with chronic ankle instability p>.01. CONCLUSION: An elastic therapeutic taping treatment given to patients with chronic ankle instability causes ankle stability to increase during a cross-cutting movement from landing.

• Journal of Pharmaceutical Investigation
• /
• 제37권3호
• /
• pp.179-186
• /
• 2007

A simple, sensitive and selective liquid chromatographic/tandem mass spectrometric method (LC-MS/MS) was developed and validated for doxifluridine and 5-fluorouracil (5-FU) quantification in dog heparinized plasma. Sample preparation was based on liquid-liquid extraction using a mixture of isopropanol/ethyl acetate (1/9 v/v) to extract doxifluridine, 5-FU and 5-chlorouracil (5-CU, an internal standard) from plasma. Chromatography was performed on a C-18 analytical column and the retention times were 2.7, 1.5 and 1.7 min for doxifluridine, 5-FU and 5-CU, respectively with shorter analysis time within 5 min than previously reported methods. The ionization was optimized using ESI negative mode and selectivity was achieved by tandem mass spectrometric analysis by multiple reaction monitoring (MRM) using the transformations of m/z 244.8>107.6, 129.0>42.0 and 144.9>42.1 for doxifluridine, 5-FU and 5-CU, respectively. The achieved low limit of quantification was 20.0 ng/mL and the assay exhibited linear range of 20-2000 ng/mL ($R^2>0.99957$ for doxifluridine and $R^2>0.99857$ for 5-FU), using $100{\mu}L$ of plasma. Accuracy and precision of quality control samples for both doxifluridine and 5-FU met KFDA and FDA Guidance criteria of 15% for accuracy with coefficients of variation less than 15%. This method demonstrated adequate sensitivity, specificity, accuracy, precision and stability to support the simultaneous analysis of doxifluridine and 5-FU in dog plasma samples in pharmacokinetic and bioequivalence studies.

• 한국실내디자인학회논문집
• /
• 제22권2호
• /
• pp.186-193
• /
• 2013

In the commercial space, the modern consumers want to consume not only product but also culture. Thus, the modern commercial space tries to induce the customer's concern and purchase to the differentiated design. The introduction of this 'the natural element' delivers the pleasure and stability to the consumer buying process and availability is enlarged. Therefore, the purpose of study is gain that expression and characteristic method of the natural element in the commercial space. The detailed study method are as follows. First, the study looks into the natural element expression tendency in the modern space. Second, the expression type of the natural element was classified as 'Reappearance', 'Transformation', and 'Fuse' based on the preceding research. And according to the content of the expression type, subdivided method of 'Inclusion' and 'Replication' of 'Reappearance' and method of 'Imitation' and 'Association' of 'Transformation' and method of 'Juxtaposition' and 'Combine' of 'Fuse'. Third, the result of analyze the characteristic of expression of the natural element of the besides the commercial space case 20 place is as follows. First, 'Inclusion' of 'Reappearance' introduced the external scenery or planned garden as the inside through the opening. 'Replication' is used for decorative purposes or functional purposes as the natural element. 'Imitation' of 'Transformation' imitated the form, pattern, and color of the natural element and was mainly expressed in the wall and objet. The method of 'Association' expressed the concept of the natural element for the whole of the space and a portion of the wall and objet repetitively. As for 'Juxtaposition', mixture of 'Reappearance' had a good visual effect, because it can be obtained the external-internal nature. As for 'Combine', mixture of 'Replication' and 'Imitation' was the most common method. Thus, the study results are expected to be utilized as base date in designing the commercial with development of the natural element application method.

• Journal of Pharmaceutical Investigation
• /
• 제35권6호
• /
• pp.431-436
• /
• 2005

A high-performance liquid chromatographic method was validated for quantitation of nadolol in human plasma. Nadolol and internal standard, pindolol, were extracted with tert-butyl methyl ether after addition of 10 M sodium hydroxide solution. The analytes were separated on a reverse phased C18 column using a mobile phase consisting of 0.05 M ammonium phosphate monobasic buffer, acetonitrile and methanol (81: 17:2 v/v/v) and detected using a fluorescence detector (excitation wavelength 230 nm, emission wavelength 294 nm). The method was specific and sensitive enough to detect as low as 3 ng/mL of nadolol in human plasma. Linear calibration range was 3-150 ng/mL with correlation coefficient greater than 0.999. The overall accuracy was in the range of 96.8 to 103% and precision C.V.(%) 7.30 to 12.2%. The recovery was approximately 100% and stability was confirmed during storage and sample preparation. The present HPLC method was successfully applied to study bioavailability after oral administration of 80 mg of nadolol in healthy Korean subjects. The mean $AUC_{t}$ was $1968{\pm}397\;ng{\cdot}hr/mL$ and $C_{max}$ of $186{\pm}79.3\;ng/mL$ was reached at $3.5{\pm}0.76\;hr$. The mean $t_{1/2}$ of nadolol was $17.3{\pm}2.59\;hr$.

• 한국항공우주학회지
• /
• 제31권2호
• /
• pp.63-71
• /
• 2003

본 논문에서는 유연우주비행체의 강인 선회기동을 위한 슬라이딩 모드 제어기법에 대해 연구하였다. 본 연구에서는 유연우주비행체의 1축 선회기동을 위한 선회목표각, 목표각속도 및 중앙동체와 유연부착물 사이에 작용하는 내부 반력 모멘트를 포함하는 슬라이딩 평면을 설계하였다. Lyapunov 제어기법과 비교한 결과 두 제어기법 모두 공칭 시스템에 대한 명령 추종성을 확인하였으며, 특히 슬라이딩 모드 제어기법의 적용결과 시스템 파라메터의 변동 및 외란의 영향에도 좋은 제어결과를 얻을 수 있음을 확인하였다. 1축 선회제어 결과를 확장하여 임의의 3축 선회기동을 수행할 수 있는 슬라이딩 모드 제어기를 설계하였다. 3축 선회기동 제어를 위해 쿼터니언을 이용하여 비행체의 자세를 결정하였고, 3개의 축 각각에 대해 설계된 슬라이딩 평면으로부터 선회기동시 발생하는 각 축간의 커플링 효과를 고려하여 제어기를 설계하였다. 설계된 제어기는 효과적인 3축 선회기동을 수행할 수 있음을 수치 시뮬레이션을 통해 보여주고 있다.