Ciliates have the possibility of a new live food in marine finfish culture because of their wide range of body size, thin tell wall, show motility, and fast reproduction rate. In this research, six species of ciliates were isolated from south coast and salt pond in Korea. The fitness of these species as a live food was evaluated in terms of size, motility, suspensibility and cell density. As the result, Euplotes sp. (K-1) was found suitable to be a new live food which might substitute rotifers, Brachionus plintilis and B. rotundiformis in fish larvae culture. The modified $F{\emptyset}yn's$ Erdschreiberd media, MErds-2 with the addition of glycine, glucose and yeast extract increased six times higher growth rate of Euplotes sp. (K-1) than the basic F$\emptyset$yn's Erdschreiberd media. The optimum water temperature, pH and light intensity for this ciliates were $22.5^{\circ}C$, 8 and 2,000 lux, respectively, and its culture environmental range was relatively wide, On the other hand, this ciliate fed baker's yeast, Saccharomyces cererisiae grew up to 1,240 inds./mL with the inocula of 100 inds./mL within 7 days. The results of the study showed that Euplotes sp. (K-1) has a potential to be utilized as a new live food in fish larvae culture.
Kim Jin-Seog;Lee Byung-Hoi;Kwon Suk-Yoon;Kim Yun-Hee;Kim So-Hee;Cho Kwang-Yun
Journal of Plant Biotechnology
/
v.32
no.2
/
pp.97-103
/
2005
Antioxidative responses of transgenic tobacco plants expressing both superoxide dismutase (SOD) and ascorbate peroxidase (APX) in chloroplasts was investigated with several herbicides. In greenhouse test, tolerance of SOD/APX-overexpressed tobacco (CA) to photosystem (PS) I inhibitor paraquat was increased by about 40%. However, any response differences between CA and wild type (WT) tobacco was not observed in a treatment with PS II inhibitors (bromoxynil, diuron and bromacil), chlorophyll biosynthesis inhibitor(oxyfluorfen), carotenoid biosynthesis inhibitor (fluridone) and 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase inhibitor (glyphosate). This tendency was also similar in the growth chamber test of low light intensity, using paraquat and diuron. That is, increased antioxidant activity of CA was shown only in paraquat treatment. When paraquat was foliar-treated to 6 to 9-leaf stage plant, the third to fourth placed leaf from shoot tip showed relatively higher antioxidant activity. Ascorbate supplemented to paraquat solution alleviated the phytotoxicity with a similar range in both CA and WT. In conclusion, CA specifically responded to oxidative stress induced by paraquat among tested herbicides in a whole plant assay.
Park, Chang-Young;Chung, Ki-Soo;Lee, Jong-Duk;Chang, In-Su;Lee, Jungil;Kim, Jang-Lyul
Journal of Radiation Protection and Research
/
v.40
no.1
/
pp.46-54
/
2015
The thermoluminescence (TL) and optically stimulated luminescence (OSL) are commonly used to measure and record the expose of individuals to ionization radiation. Design and performance test results of a newly developed TL and OSL measurement system are presented in this paper. For this purpose, the temperature of the TL material can be controlled precisely in the range of $1{\sim}1.5^{\circ}C$ by using high-frequency (35 kHz) heating system. This high-frequency power supply was made of transformer with ferrite core. For optical stimulation, we have completed an optimal combination of the filters with the arrangement of GG420 filter for filtering the stimulating light source and a UG11 filter at the detecting window (PMT). By using a high luminance blue LED (Luxeon V), sufficient luminous intensity could be obtained for optical stimulation. By using various control boards, the TL/OSL reader device was successfully interfaced with a personal computer. A software based on LabView program (National Instruments, Inc.) was also developed to control the TL/OSL reader system. In this study, a multi-functional TL/OSL dosimeter was developed and the performance testing of the system was carried out to confirm its reliability and reproducibility.
Yoon, Jin-Hwan;Lee, Hee-Hyuk;Kim, Jong-Oh;Oh, Myung-Jin;Park, Seong-Tae;Jee, Young-Seok;Seo, Tae-Beom;NamGung, Uk
Journal of Life Science
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v.17
no.6
s.86
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pp.859-866
/
2007
The main objective of this study was to investigate the effects of exercise on serum insulin and leptin levels and GLUT-4 and VAMP-2 in skeletal muscles from the pregnant rats. F344 rats were randomly divided into four groups (n = 15 in each group): control group (CG), pregnant group (PG), pregnant running group (PR), and pregnant swimming group (PS). From the 15th day of pregnancy, animals in the running group were forced to run on treadmill for 30 min with a light intensity, while those in the swimming group were forced to swim in swimming pool for 10 min once a day for 6 consecutive days. The present result demonstrated that in pregnant rat group, serum insulin levels significantly in-creased and leptin levels significantly decreased. Skeletal GLUT-4 and VAMP-2 protein expression was significantly decreased in pregnant rats compared to non-pregnant rats. However, matenal running during gestational period alleviated pregnancy-induced changes in plasma insulin and leptin levels, and it significantly enhanced skeletal GLUT-4 and VAMP-2 protein expression. From those results, it can be suggested that running exercise during gestational period may improve glycemic control by up-regulating GLUT-4 and VAMP-2 protein expression.
This study describes about the spatio-temporal distributions in phytoplankton community such as species composition, standing crop and dominant species from May 2006 to February 2007 in the Jangsu bay and the northwestern parts of Gamak bay. Based on the principal component analysis (PCA) of the environmental factors as well as biological parameters, the bio-oceanographical characteristics were analysed. A total of 83 species of phytoplankton belonging to 47 genera were identified. Whereas diatoms and dinoflagellates occupied more than 65% and 30% of total species, respectively. The annual dominant species were Chaetoceros affinis, Paralia sulcata and Bacillaria paxillifera in spring, Chaetoceros didymus, Ch. affinis and Octactis octonaria in summer, Skeletonema costatum-like species and B. paxillifera in autumn. Moreover phytoplankton cell density was ranged between 3.1 $cells{\cdot}mL^{-1}$ in spring and 521.0 $cells{\cdot}mL^{-1}$ in winter. It fluctuated with an annual mean of 76.0 $cells{\cdot}mL^{-1}$ between the lowest value of 7.6 $cells{\cdot}mL^{-1}$ in spring and the highest value of 220.2 $cells{\cdot}mL^{-1}$ by Skeletonema costatum-like species in winter. Briefly, the phytoplankton cell density in the mixing seasons was higher in comparison with the other seasons. According to the PCA, the biological oceanographic characteristics of the Jangsu bay was affected by the introduction of outside seawater particularly in temperature increasing seasons, and the other seasons, it may be described the light intensity, and mix between inner and outer bay sea waters.
Pleurotus eryngii(King oyster) is one of the most promising mushrooms produced on the domestic farms. The quality as well as quantity of Eryngii is sensitively affected by micro climate factors such as temperature, relative humidity, $CO_2$ concentration, and light intensity. To safely produce high-quality Eryngii all the year round, it is required that the environmental factors be carefully controlled by well designed structures equipped with various facilities and control systems. At the commercial mushroom cultivation house(A,B), this study was carried out to find out reasonable range of each environmental factor and yield together with economic and safe structures influencing on the optimal productivity of Eryngii. this experiment was conducted for about two-month from Nov. 11, 2002 to Dec. 30, 2002 in Eryngii. cultivation house-A, B. Ambient temperature during the experiment period was not predominantly different from that of a normal year. The capacity of the hot water boiler and the piping systems were not enough. Maximum air temperature difference between the upper and lower growth stage during a heating time zone was about 2~3$^{\circ}C$. The max. and min. relative humidity were ranged approximately 60~100%, and average relative humidity was ranged approximately 80~100%. And $CO_2$concentration increased until maximum 1,600~1,800 ppm with the passing growing period. The illuminance in cultivation house was widely distributed from 20lx to 160 lx in accordance with position, and it was maintained lower than the recommended illuminance range 100~200 lx. The average yield per bottle was about 67~85g. But the optimal productivity will be evaluated by considering the quality and quantity of mushroom production, energy requirements, facility construction and management cost, etc.
Proceedings of the Korean Society of Medical Physics Conference
/
2002.09a
/
pp.400-403
/
2002
The fundamental experiments for measuring soft x-ray characteristics from the vacuum capillary are described. These experiments were primarily performed in order to generate line spectra such as x-ray lasers. The generator consists of a high-voltage power supply, a polarity-inversion ignitron pulse generator, a turbo-molecular pump, and a radiation tube with a capillary. A high-voltage condenser of 200 nF in the pulse generator is charged up to 20 kV by the power supply, and the electric charges in the condenser are discharged to the capillary in the tube after closing the ignitron. During the discharge, weakly ionized plasma forms on the inner and outer sides of a capillary. In the present work, the pump evacuates air from the tube with a pressure of about 1 mPa, and a demountable capillary was developed in order to measure x-ray spectra according to changes in the capillary length. In this capillary, the anode (target) and cathode elements can be changed corresponding to the objectives. The capillary diameter is 2.0 mm, and the length is adjusted from 1 to 50 mm. When a capillary with aluminum anode and cathode electrodes was employed, both the cathode voltage and the discharge current almost displayed damped oscillations. The peak values of the voltage and current increased when the charging voltage was increased, and their maximum values were -10.8 kV and 4.7 kA, respectively. The x-ray durations observed by a 1.6 ${\mu}$m aluminum filter were less than 30 ${\mu}$s, and we detected the aluminum characteristic x-ray intensity using a 6.8 ${\mu}$m aluminum filter. In the spectrum measurement, two sets of aluminum and titanium electrodes were employed, and we observed multi-line spectra. The line photon energies seldom varied according to changes in the condenser charging voltage and to changes in the electrode element. In the case where the titanium electrode was employed, the line number decreased with corresponding decreases in the capillary length. Compared with incoherent visible light, these rays from the capillary were diffracted and diffused greatly after passing through two slits.
This study examined Platanus orientalis and Ulmus davidiana planted in downtown parks to identify the correlations among microclimatic factors such as temperature in the crown, air flow, and wind speed. For the field survey, measurements were taken at 1 hour intervals from 09:00 am to 06:00 pm in August. For the measurement of microclimatic factors, data on temperature, light intensity, air flow, and wind speed were collected using a quantum sensor (PAR Quantum Sensor SKP215), a precision thermometer (Pt1000-Sensor), and a combination anemometer (1467 G4 & HG4). The results of the analysis demonstrated that both Platanus orientalis and Ulmus davidiana, showed a greater cooling effect inside the crown as compared with the outside temperature. The cooling effect inside the crown was more evident with air flow and wind speed factors. With relation to wind, the inner temperature of the crown of Platanus orientalis decreased due to air flow while that of Ulmus davidiana decreased due to wind speed. With no wind, the average variation in temperature inside the crown was $-0.9^{\circ}C$ for Ulmus davidiana and $-0.958^{\circ}C$ for Platanus orientalis, indicating that Platanus orientalis was relatively more effective in lowering the temperature of the planting space than Ulmus davidiana. This study is significant because it shows that different tree species have different effects on the microclimate and that factors affecting the formation of the microclimate of trees may vary with species. Further studies on species other than broad leaf trees, such as evergreen trees and shrubs, are required in order to plan the distribution of landscaping trees that are effective in regulating the microclimate within urban green spaces.
Specular reflections provide the visual feedback that describes the material type of an object, its local shape, and lighting environment. In photorealistic rendering, there have been a number of research available to render specular reflections effectively based on a local reflection model. In traditional cel animations and cartoons, specular reflections plays important role in representing artistic intentions for an object and its related environment reflections, so the shapes of highlights are quite stylistic. In this paper, we present a method to render and control stylized specular reflections using projective textures based on principal curvature analysis. Specifying a texture as a pattern of a highlight and projecting the texture on the specular region of a given 3D model, we can obtain a stylized representation of specular reflections. For a given polygonal model, a view point, and a light source, we first find the maximum specular intensity point, and then locate the texture projector along the line parallel to the normal vector and passing through the point. The orientation of the projector is determined by the principal directions at the point. Finally, the size of the projection frustum is determined by the principal curvatures corresponding to the principal directions. The proposed method can control the position, orientation, and size of the specular reflection efficiently by translating the projector along the principal directions, rotating the projector about the normal vector, and scaling the principal curvatures, respectively. The method is be applicable to real-time applications such as cartoon style 3D games. We implement the method by Microsoft DirectX 9.0c SDK and programmable vertex/pixel shaders on Nvidia GeForce FX 7800 graphics subsystems. According to our experimental results, we can render and control the stylized specular reflections for a 3D model of several ten thousands of triangles in real-time.
Cheon, Si Nae;Yoo, Geum Zoo;Jung, Ji Yeon;Kim, Chan Ho;Kim, Dong-Hoon;Jeon, Jung Hwan
Korean Journal of Organic Agriculture
/
v.29
no.2
/
pp.209-221
/
2021
The purpose of this study is to obtain basic data on housing facilities and management of broiler welfare certified farms in Korea. We investigated breeds, flock sizes, stocking density, perches, litter, plumage condition, and other diseases. In addition, we measured temperature, relative humidity, light intensity, ammonia, and carbon dioxide concentration in the barn. As result, criteria were met in all cases that we investigated. However, farmers commonly demanded relaxation of perch and litter. Perch usage of broiler was impractical due to low usage of it. Also, litter was increased, resulting in farmers' economic burden by the imbalance between supply and demand. This situation makes farmers reuse the litter. Unfortunately, there are no clear certification standards. During re-inspection, the animal welfare certification of farms was canceled due to the reuse of litter. It is difficult to modify the standard of perch due to the strong declarative meaning of animal welfare rather than the necessity of perch usage, however, the reuse of litter should be improved. It is important to think and solve any problems faced by all farms. Especially, animal welfare standards need to be improved in more clarity and rationality.
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