• Mass Spectrometry Letters
• /
• 제6권3호
• /
• pp.80-84
• /
• 2015

Here, we demonstrate the use of MALDI-TOF as a fast and simple analytical approach to evaluate the DNA-binding capability of various peptides. Specifically, by varying the amino acid sequence of the peptides consisting of lysine (K) and tryptophan (W), we identified peptides with strong DNA-binding capabilities using MALDI-TOF. Mass spectrometric analysis reveals an interesting novel finding that lysine residues show sequence selective preference, which used to be considered as mediator of electrostatic interactions with DNA phosphate backbones. Moreover, tryptophan residues show higher affinity to DNA than lysine residues. Since there are numerous possible combinations to make peptide oligomers, it is valuable to introduce a simple and reliable analytical approach in order to quickly identify DNA-binding peptides.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2006년도 하계종합학술대회
• /
• pp.975-976
• /
• 2006

The passive type RFID (Radio Frequency Identification) System using Surface Acoustic Wave (SAW) tag at 900 MHz in the range of more than 1 m was fabricated. To improve interrogation range of the system propose a method to increase isolation between transmitter and receiver. This method using a direct conversion architecture achieves a leakage rejection of 10 dB increased compared with conventional system. Measured interrogation range is more than 1 m.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2006년도 하계종합학술대회
• /
• pp.469-470
• /
• 2006

Surface Acoustic Wave(SAW) devices can be used to as wireless sensor elements, called SAW transponders, for measuring shysical quantities such as temperature that do not need any power supply and may be accessed wirelessly. SAW devices were fabricated on Y-Z $LiNbO_3$ piezoelectric substrate with a good temperature coefficient property. The signal response of SAW sensor on the temperature change were compared. To measure the change of SAW velocity. Temperature changed form $20^{\circ}C$ to $400^{\circ}C$ was linearly changed, the SAW sensor is application to the temperature sensor.

• Bulletin of the Korean Chemical Society
• /
• 제29권9호
• /
• pp.1673-1677
• /
• 2008

• 한국가정과교육학회지
• /
• 제22권3호
• /
• pp.163-188
• /
• 2010

이 연구는 일상의 문제를 통합적인 사고능력으로 풀 수 있는 새로운 교수 - 학습 방법의 하나로 주제중심 통합적 접근에 의한 가정교과 의생활 영역 교육 프로그램을 개발하고 평가하는데 목적을 두었다. 이를 위해 가정교과 의생활 영역의 주제를 선정하였고, 주제를 중심으로 통합적 접근을 시킨 교육 프로그램을 개발하고 개발된 프로그램을 평가하였다. 연구의 절차는 부산광역시 소재 B중학교 2학년 학생을 대상으로 총 28시간의 현장수업에 적용하여 평가하였다. 분석 단계에서 다른 교과의 통합적 접근에 관련된 프로그램을 탐색한 후, 설계 단계에서 프로그램의 유행을 파악하고, 개발 단계에서는 주제 선정 기준에 따라 주제를 선정을 하였다. 주제선정을 위해 선행연구와 기술 가정 교과서를 분석하였고, 학생들과의 메신저, 전문가 집단의 논의를 거쳐서 2개의 "의복관리"와 "섬유(재료)"를 선정하였다. 평가는 1차 전문가평가, 2차 예비 수업 평가, 3차 현장수업 평가를 하였다. 연구의 결과는 2개의 주제에 대해 주제중심 통합프로그램을 개발하였으며 "의복관리"편의 학습 자료는 총 49가지, "섬유(재료)"편의 핵습 자료는 총 32가지이며, 주제의 성격에 맞게 실험과 실습 등으로 구성하였다. 각 프로그램은 프로그램의 목표 학습내용 내용구조도 학습활동 평가로 이루어져 있고 학습활동은 체험 실습 및 실험활동 자기 주도적 활동 문제해결과정 등으로 구성되었으며, 평가는 자기평가 교사평가 동료평가 포트폴리오 실험보고서 등이 포함되었다. 1, 2차 평가에서 본 프로그램이 현 시점의 요구와 잘 부합되며, 타 교과와의 융합이 수업을 진행하는 데 적절하게 구성되어 있고, 학습자의 흥미도 지속적으로 유지할 수 있다고 평가하였다. 다만 프로그램의 내용 각 부분에 대해서 구체적인 설명이 필요하다고 요구하였다. 3차 학생 현장 수업평가에서 약 60%의 학생들이 개발된 프로그램이 재미있고, 내용을 이해하기 쉬우며, 한 가지 주제가 여러 과목과 관련이 있다는 사실을 알게 되었고 실제 생활에 적용 가능할 것이라고 하였다. 이상의 결과로서 주제중심 통합적 접근에 의한 가정교과 의생활 영역 교육 프로그램은 주제를 중심으로 통합적으로 접근을 시도했다는데 의의가 있다. 또한 학생들은 지식을 통합적으로 모으는 일 뿐 아니라 이를 창조적으로 변화시켜 실제 생활에서 직면하게 되는 여러 문제를 해결하는데 응용할 수 있었다. 이 연구를 많은 교사들이 활용하여 학생들이 실생활에서 접하는 문제를 통합적으로 사고하여 해결을 할 수 있기를 기대한다.

• 항공우주시스템공학회지
• /
• 제9권3호
• /
• pp.39-46
• /
• 2015

An integrated simulation program for an artificial satellite's operation has been developed. The program integrates and simulates orbit mechanics, attitude control, power/energy transition and mass variation. In the early stages of satellite development, this program can be used as a communication tool among design engineers of different fields. As a result, the efficiency to design a satellite is expected to increase. This program has been coded by Modelica language which supports acausal and object oriented programming methods. Libraries are developed for satellite simulation, and simulation results are presented.

• 한국의상디자인학회지
• /
• 제19권2호
• /
• pp.49-61
• /
• 2017

The purpose of this study was to develop an educational program designed to allow students to experience 'up-cycling' first-hand during class and understand its significance by helping them improving their practical problem-solving abilities. Teachers of home economics, Korean, and social studies to third grade middle schooler were asked to analyze the curriculums of their subjects. The analysis results were then used to identify common elements among the units, reconstruct the curriculums, and develop an integrated lesson program to offer integrated activities. Based on these, a lesson program was developed to make an eco-bag and running shoes under the theme of 'up-cycling' in the unit of 'environmentally-friendly clothing and mending of clothes' in the home economics subject. The results were as follows: First, a topical fusion lesson program was developed to integrate three subjects together. The development process involved the integration of the topic of up-cycling, a program model for integrated lessons, and a teaching and learning process plan for topical integrated education. Secondly, a lesson program for home economics was developed that was applicable to actual home economics lessons based on the topical integrated lesson program. The lesson domains were divided into large, medium, and small to create a ten-lesson teaching and learning process plan needed for the lessons, teaching materials that could be put to actual uses in lessons, and activity and evaluation logs for learners.

• 한국초등과학교육학회지:초등과학교육
• /
• 제32권4호
• /
• pp.473-484
• /
• 2013

The purpose of this study was to develop science and art integrated program to improve the creativity of scientifically gifted elementary school students. This study was to develop science and art integrated program to enhance the creativity of these subjects. This program was consisted of 30 lessons covering 10 topics. It was developed of five stages including the observation stage reflecting the characteristics of the right hemisphere relevant to creativity, the interest and curiosity stage, the experiment design and performing stage, the internalization stage, and the stage of expressing arts. This program was applied to 20 senior gifted students in Y Elementary School in Gyeonggi province. Torrance Tests of Creative Thinking(TTCT) was used in order to investigate and measure the effectiveness of the program before and after its use in class. The results of this study are as follows: First, this program showed results of significant improvement of creativity of scientifically gifted elementary school students after its use in class(p<.05). Second, it was significantly effective in increasing their creativity, especially in the subdomains such as originality, abstractness of title, and territory of resistance on hasty conclusions after its use in class(p<.05). Third, it was significantly effective to increase the Creativity Index that represents creative potential(p<.01). In particular, emotional expression, internalized visualization, unique visualization, and richness of the imagery emerged. This study implies that the science and art integrated program was closely related to the right hemisphere of the features enabling the subjects to create new ideas, new things, and new reactions. In addition, this program is expected to contribute to activate the brain areas of creativity for gifted students in the science field.

• Bulletin of the Korean Chemical Society
• /
• 제32권4호
• /
• pp.1170-1178
• /
• 2011

In the present study, the approach of high accuracy mass measurements for phospholipid identifications was evaluated using a 7 T ESI-FTMS/linear ion trap MS/MS. Experiments were carried out for porcine brain, bovine liver, and soybean total lipid extracts in both positive and negative ion modes. In total, 59, 55, and 18 phospholipid species were characterized in the positive ion mode for porcine brain, bovine liver, and soybean lipid extracts, respectively. Assigned lipid classes were PC, PE, PEt, PS, and SM. In the negative ion mode, PG, PS, PA, PE, and PI classes were observed. In the negative ion mode, for porcine brain, bovine liver, and soybean lipid extracts, 28, 34, and 29 species were characterized, respectively. Comparison of our results with those obtained by other groups using derivatization-LC-APCI MS and nano-RP-LC-MS/MS showed that our approach can characterize PC species as effectively as those methods could. In conclusion, we demonstrated that high accuracy mass measurements of total lipid extracts using a high resolution FTMS, particularly, 7T FTMS, plus ion-trap MS/MS are very useful in profiling lipid compositions in biological samples.

• Journal of Microbiology and Biotechnology
• /
• 제18권1호
• /
• pp.35-42
• /
• 2008

Vibrio vulnificus produces siderophores, low-molecular-weight iron-chelating compounds, to obtain iron under conditions of iron deprivation. To identify genes associated with the biosynthesis of siderophore in V. vulnificus MO6-24/O, we screened clones with mini-Tn5 random insertions for those showing decreased production of siderophore. Among 6,000 clones screened, nine such clones were selected. These clones contain the transposon inserted in VV2_0830 (GenBank accession number) that is a homolog of a nonribosomal peptide synthase (NRPS). There is an another NRPS module, VV2_0831, 49-bp upstream to VV2_0830. We named these two genes vvs (Vibrio vulnificus siderophore synthase) A and B, respectively. Mutation of either vvsA or vvsB showed a decreased production of siderophore. The expression of an NRPS-lux fusion was negatively modulated by the presence of iron, and the regulation was dependent on Fur (ferric uptake regulator). However, the expression of the NRPS genes was still not fully derepressed in the iron-rich condition, even in furnull mutant cells, suggesting that some other unknown factors are involved in the regulation of the genes. We also demonstrated that the NRPS genes are important for virulence of the pathogen in a mice model.