• Title/Summary/Keyword: integrated DB

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Expression and Regulatory Analysis of Sporulation Gene (spo 5) in Schizosaccharomyces pombe (Schizosaccharomyces pombe 포자형성유전자 (spo 5)의 발현조절기구의 해석)

  • KIM Dong-Ju;SHIMODA Chikasi
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.30 no.1
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    • pp.46-54
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    • 1997
  • Sporulation in the fission yeast Schizosaccharomyces pombe has been regarded as an important model of cellular development and differentiation. S. pombe cells proliferate by mitosis and binary fission on growth medium. Deprivation of nutrients especially nitrogen sources, causes the cessation of mitosis and initiates sexual reproduction by malting between two sexually compatible cell types. Meiosis is then followed in a diploid cell in the absence of nitrogen source. DNA fragment complemented with the mutations of sporulation gene was isolated from the S. pombe gene library constructed in the vector, pDB 248' and designated as pDB (spo 5)1. We futher analyzed six recombinant plasmids, pDB (spo 5)2, pDB(spo 5)3, pDB(spo 5)4, pDB(spo 5)5, pDB(spo 5)6, pDB(spo 5)7, and found each plasmids is able to rescue the spo 5-2, spo 5-3, spo 5-4, spo 5-5, spo 5-6, spo 5-7, mutations, respectively. Mapping of the integrated plasmid into the homologous site of the S. pombe chromosomes demonstrated that pDB (spo 5)1, and pDB (spo 5)R1 contained the spo 5 gene. Transcipts of spo 5 gene were analyzed by Northern hybridization. Two transcripts of 3.2 kb and 25 kb were detected with 5 kb Hind III fragment containing a part of the spo 5 gene as a probe. The small mRNA (2.5 kb) appeared only when a wild-type strain was cultured in the absence of nitrogen source in which condition the large mRNA (3.2 kb) was produced constitutively. Appearance of a 2.5 kb spo 5-mRNA depends upon the function of the mei1, mei2 and mei3 genes.

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The Overexpression of Subtilisin Enzyme Using Mutations on Transition State Regulatory Proteins of AprE Promoter and Development of Bacillus subtilis Host System (AprE Promoter전이상태 조절인자 변이주를 이용한 공업적 효소의 과발현과 고초균 숙주계의 개발)

  • 류성호;박승환김병기
    • KSBB Journal
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    • v.11 no.1
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    • pp.8-14
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    • 1996
  • Bacillus subtillis strains with transition state regulator mutations and a spore mutation were developed for the overexpression of apsE and for the enhancement of expression level. Among the many regulator genes, degU and hpr were chosen as a representative positive and negative regulator for the aprE, respectively. Spo II G was used for the construction of asporogeneous strains. All the mutants were constructed from two protease-deleted strain DB104 and the apsE gene was transformed with an integration vector pMK101. DB104(deg$U^h$(32) $his^+$)::pMK101(Cm) and DB104($\Delta$her(Em))::pMKl01(Cm) show 7-fold and about 2-fold increase in aprE expression level, respectively. But the effect of transition state regulator mutation on the aprE expression was diminished when the integrated aprE gene was amplified by the high concentration of chloramphenicol, i. e. 30 $\mu\textrm{g}$/ml. DB104($\Delta$spoIIG(Pm) degUh(32) his+)::pMK101(Cm) and DB104($\Delta$spoIIG(Pm) $\Delta$hpr(Em))::pMK101 double mutant show 10-fold and 3-fold increase in aprE expression level, respectively. The results suggest that sporulation mutation and transition state regulator mutation have independent and additive effect on the aprE expression, and the same gene dosage effect on the transition state regulator mutation was also identified.

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A Study on Project Information Integrated Management Measures Using Life Cycle Information in Road Construction Projects (도로건설사업의 생애주기별 정보를 이용한 건설사업정보 통합관리방안 연구)

  • Kim, Seong-Jin;Kim, Bum-Soo;Kim, Tae-Hak;Kim, Nam-Gon
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.11
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    • pp.208-216
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    • 2019
  • Construction projects generate a massive amount of diverse information. It takes at least five years to more than 10 years to complete, so it is important to manage the information on a project's history, including processes and costs. Furthermore, it is necessary to determine if construction projects have been carried out according to the planned goals, and to convert a construction information management system (CALS) into a virtuous cycle. It is easy to ensure integrated information management in private construction projects because constructors can take care of the whole process (from planning to completion), whereas it is difficult for public construction projects because various agencies are involved in the projects. A CALS manages the project information of public road construction, but that information is managed according to CALS subsystems, resulting in disconnected information among the subsystems, and making it impossible to monitor integrated information. Thus, this study proposes integrated information management measures to ensure comprehensive management of the information generated during the construction life cycle. To that end, a CALS is improved by standardizing and integrating the system database, integrating the individually managed user information, and connecting the system with the Dbrain tool, which collectively builds artificial intelligence, to ensure information management based on the project budget.

The design of 4s-van for GIS DB construction (GIS DB 구축을 위한 4S-VAN 설계)

  • Lee, Seung-Yong;Kim, Seong-Baek;Lee, Jong-Hun
    • Journal of Korean Society for Geospatial Information Science
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    • v.10 no.3 s.21
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    • pp.89-97
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    • 2002
  • We have developed the 45-Van system in order to maximize the interoperability of spatial data in 45(GNSS, SIIS, GIS, ITS) by sharing and providing spatial data of remote site. The 4S-Van system enables to acquisition and production of information for GIS database and the accurate position information by combining and connecting GPS/IMU, laser, CCD(charged-coupled device) image, and wireless telecommunication technology. That is, 4S-Van system measures its position and attitude using integrated GPS/IMU and takes two photographs of the front scene by two CCD cameras, analyzes position of objects by space intersection method, and constructs database that has compatibility with existing vector database system. Furthermore, infrared camera and wireless communication technique can be applied to the 4S-Van for a variety of applications. In this paper, we discuss the design and functions of 4S-Van that is equipped with GPS, CCD camera, and IMU.

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Design of Customized Medical Information Convergence System for the Glycosuria and Heart's Blood Patients (당뇨 및 심혈관 질환자를 위한 개인 맞춤형 의료정보 융합시스템 설계)

  • Kim, Gui-Jung;Han, Jung-Soo
    • The Journal of the Korea Contents Association
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    • v.9 no.9
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    • pp.90-96
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    • 2009
  • The aim of the research is that disease database of the glycosuria and heart's blood is designed to manage the condition of the glycosuria and heart's blood patients periodically and continuously. Also we integrate patient database of existing OCS, PACS, EMR, ERP etc. and support optimal service timely that the patients want through intelligent integrated interface environment. For this, we will develop customized medical information convergence system. We construct intelligent database for disease of the glycosuria and heart's blood. And we support data integration environment for connection with existing systems - OCS, EMR, PACS etc. Also, in consideration of QoS, reliability, and expandability of customized medical information convergence system, we will design H/W, S/W, and data compatibility method.

Analysis of the Effects of Job Policy Measures in Korea: Do the job policy measures impact the marriage and fertility of the youth in Korea?

  • Kang, Chang Ick;Lim, Kyung Eun;Kim, Junghak
    • Asian Journal for Public Opinion Research
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    • v.10 no.3
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    • pp.200-229
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    • 2022
  • The purpose of this study is to analyze the effects of youth job policy measures, set forth in Korea's 2016-2020 Third Basic Plan for Low Fertility and Aging Society (December 2015), on marriage and fertility among young people. Based on the results, we provide theoretical explanations for the findings and suggest policy alternatives to overcome the low fertility phenomenon in Korea. Previous studies have shown that employment is an important factor for marriage among youth, and a job policy could increase marriage and fertility rates. To test this assumption, we performed an exact matching between Statistics Korea's Employee-Enterprise Linkage DB and the Newlyweds DB from 2011 to 2019, in order to identify all young people aged 15-34. Then, linear spline regression analysis was used to examine the impact of the youth job policy on marriage and fertility. Comparing the period before the implementation of the employment policy (2011-2015) and after (2016-2019), the fertility rate increased as the number of young people looking for work increased. In addition, it was found that these impacts were greater after the implementation of the measures (2016-2019) than before (2011-2015). It is interesting to note that job growth among young people did not lead to an increase in marriage. However, the number of births significantly increased when young people who occupy jobs got married, which seems to be related to the delay in marriage among young people who are employed. Survey results about the intentions to marry and views on fertility are utilized for the explanation of the study results.

A study on the collection of river water usage using IRDiMS (IRDiMS를 활용한 하천수 사용량 수집 방안에 관한 연구)

  • Sang Uk Cho;Dong Heon Oh;Jong Seok Baek;Jeong Hwan Cheon
    • Proceedings of the Korea Water Resources Association Conference
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    • 2023.05a
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    • pp.440-440
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    • 2023
  • 하천수 사용량 자료는 하천법에 따라 사용자가 월단위로 사용량(농업용수 8,000m3/s 이상)을 한강홍수통제소 하천수사용관리시스템에 직접 입력 또는 우편이나 공문 등으로 전달되어 입력되고 있다. 하지만 현재와 같이 월단위로 수집되는 자료로는 하천수 사용량 통계자료 분석이나 효율적인 물관리를 위한 하천 유량 관리에는 활용에 한계가 있어 정확한 하천수 사용량 관리가 어렵다. 따라서, 본 연구에서는 현장에서 계측되어 표준화된 자료로 전송되는 자료를 현재 한강홍수통제소에서 운영중인 첨단 계측장비를 이용해 하천 유량 자료를 실시간으로 수집 및 관리하고 있는 IRDiMS(Integrated Real-time Discharge Measurement System)를 활용하여 하천수사용관리시스템에 하천수 사용량 자료를 실시간으로 입력하도록 개선방안을 제시하고자 하였다. IRDiMS는 계측장비를 통해 하천 유량 자료를 수집하여 품질관리를 거쳐 하천의 유량자료를 관리하고 있어 실시간 하천수 사용량 자료는 IRDiMS 의 처리체계를 동일하게 활용할 수 있다. 또한 IRDiMS에서 처리된 자료는 물리적·소프트웨어 측면에서 한강홍수통제소 내부DB와 같은 공간에 위치하여 자료를 하천수사용관리시스템 외에도 국가수문DB 및 타시스템에 연계하는데 유리하다. 실시간 기반의 하천수 사용량 자료는 현장에서 실시간으로 계측되어 산정된 하천수 사용량 자료를 일정한 형식에 따라 10분 주기로 IRDiMS의 수신서버로 전송하고 수집된 자료를 처리서버에서 품질관리를 거친 후 자동유량DB에 저장된다. 저장된 자료는 정해진 규칙에 따라 국가수문DB로 자동 연계하고, 실시간으로 외부망에 있는 연계서버를 통해 하천수사용관리시스템DB에 자료를 저장하도록 하천수 사용량 수집에 대한 방안을 마련하였다.

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A Development of Integrated Map Preprocessor for Mobile GIS Visualization (Mobile GIS 가시화를 위한 Integrated Map Preprocessor 개발)

  • Jeon, Chang-Young;Park, Jun;Song, Eun-Ha;Jeong, Young-Sik
    • Annual Conference of KIPS
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    • 2005.05a
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    • pp.707-710
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    • 2005
  • 과거 지리정보시스템 서비스들은 단일 어플리케이션에서 동작되어 왔다. 데이터 처리 기술의 급속한 발전과 컴퓨터 및 Mobile 사양이 높아지면서 사용자들은 각각의 지리정보시스템 자체 데이터는 물론, 이질적인 다른 여러 지리정보시스템의 통합 가시화와 효율적인 공유를 요구하고 있다. 그러나, 많은 지리정보시스템 어플리케이션들은 자체 데이터 포맷과 상이한 데이터 포맷에 대해서 처리하지 못하는 단점을 가지고 있다. 본 논문에서는 지리정보시스템의 대표적인 형식이고 국립지리원에서 제공하고 있는 DXF(Drawing eXchange Format)파일 포맷과 현재 사용되고 있는 지리 정보인 DWG(DraWinG), SHP(SHaPefile)등의 포맷을 통합된 하나의 뷰어를 통해 가시화하기 위한 IMP(Integrated Map Preprocessor)를 설계한다. 또한 Mobile 지리정보시스템 환경에서의 데이터 활용을 효과적으로 하기 위해 추출된 정보를 공통된 기준의 의미부여로 인덱싱화하여 DB에 저장한다.

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Design of Integrated Database Program for Optimal Operation of Distribution Automation System (배전지능화시스템 최적 운영을 위한 통합 데이터베이스 프로그램 설계)

  • Choi, Yun-Hyuk;Jang, Moon-Jong
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.8
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    • pp.987-993
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    • 2018
  • In the existing distribution automation system, the database is managed by each distribution office. But there is a high possibility to happened a problem during a fault processing because the representation of the connection switches between distribution offices are complicated. In addition, if a distribution office will be going to collapse due to disaster, for example, a big fire or earthquake, its own database may be lost. In order to solve this problem, it is necessary to have a structure of an integrated database management from an integration center to under the distribution office. To do this, the integration center must store and manage it as the database using the own office code.

Design of Standard Submission Format for Underground Structures : An Automated Update of the UnderSpace Integrated Map (지하공간통합지도 자동갱신을 위한 지하구조물 제출 표준 설계)

  • Park, Dong Hyun;Jang, Yong Gu;Ryu, Ji Song
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.39 no.6
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    • pp.469-476
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    • 2021
  • The framework plan for the development of an integrated underground space map was established of preventing ground subsidence. The mapping process is expected to be completed to the level of nationwide municipal government standards by end of this year. To facilitate the utilization of the integrated underground space map, paper-based drawings for specialized organizations in underground safety impact assessment have been provided since September 2018, and services for local government officials have been provided in the underground information utilization system since May 2019. However, the map is utilized based on the information at the time of the initial development of the map, without any updates, thereby resulting in a lack of accuracy and latest information. This has led to a decrease in the utilization and reliability of the information. Therefore, in this study, for the underground structures(subway, underground shopping mall, underground passage, underground roadway, underground parking lot, utility tunnel), which are the key components of the integrated underground space map, a standard format for the submission of completed drawings is designed in accordance with Article 42 (2) of the Special Act on Underground Safety Management, which aims at laying the foundation for establishing the updated system of the integrated underground space map. In addition, through the verification of the automatically updated underground structure data based on the standard format, the reliability of the data can be assured. This format is expected to contribute to the improved utilization of the integrated underground space map in the future.