• 대한수의학회지
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• 제35권1호
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• pp.55-65
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• 1995

Ultrastructures of pancreatic endocrine cells containing glucagon, insulin, somatosratin and pancreatic polypeptide were studied in the pancreas of the Korean native goat by immunohistochemical and elecron microscopy. Glucagon immunoreatctive cells were round or fusiform in shape and contained secretory granules of 200-260 nm in diameter. The secretory granules were high in electron density and had a halo between the limiting membrane and the central granule core. Insulin immunoreactive cells were round or oval in shape, and contained various sizes of secretory granules from 135 to 300 nm in diameter. The secretory granules were low or moderate electron density and had a variform halo. Somatostatin immunoreactive cells were elliptical or fusiform shape with cytoplasmic processes. They contained the secretory granules of 140-320 nm with moderate electron densities. Pancreatic polypeptide immunoreactive cells were elliptical or fusiform and contained small secretory granules with high electron densities. The secretory granules were 120-230 nm in diameter and the least in number.

• Applied Microscopy
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• 제25권1호
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• pp.122-129
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• 1995

Pancreatic endocrine cells of the native Korean goat were investigated immunocytochemically at electron microscopic level. All glucagon-, insulin-, somatostatin- and pancreatic polypeptide(PP)-immunoreactive cells were showed chromogranin(CG) immunoreactivity in the secretory granules of each cells. In addition, bovine pancreatic polypeptide immunoreactivity was found to be colocalized in the secretory granules of the glucagon and insulin cells. These observations support that chromogranin is available as the marker of pancreatic endocrine cells on the native Korean goat and BPP colocalized in the secretory granules of glucagon and insulin cells.

• 대한본초학회지
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• 제23권1호
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• pp.63-74
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• 2008

Objectives : This study has been carried out to understand the effect of Uncooked Korean Food(F-DM) on blood glucose and antioxidant enzyme activities in streptozotocin-induced Diabetic Rats. Methods : SD rats were separated into four groups(each with 20 rats). Except normal two group, the other two groups were injected into intra-peritoneal with streptozotocin 60 mg/kg. Experimental group was eated Feed with 25% F-DM for 4 weeks. The change of plasma glucose level, body weight were observed. After 4 weeks, liver and kidney weight, antioxidant enzyme activities, survival rate were observed with histological changes on liver, kidney and pancreas. Results : In experimental group, body weight and survival rate increased, plasma glucose level were decreased significantly. Liver and kidney weight, XOD activity were decreased in experimental group compared to control group. GSH-px and CAT activities, insulin- immunoreactive granules in p-cells were increased significantly in experimental group compared to control group. Conclusions : This study shows that the F-DM might be effective for treatment of diabetes and its complications, as well as reduction of the oxidative stress.

• Applied Microscopy
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• 제31권3호
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• pp.267-274
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• 2001

Streptozotocin(STZ)을 투여하여 고혈당을 유발시킨 생쥐의 이자섬에 생진양혈탕가미방이 미치는 영향을 규명하고자, 전방액 (0.4ml/day)을 21일간 투여하여 혈당 및 BUN의 변화와 인슐린 면역조직화학적 검색 및 전자 현미경을 이용한 $\beta$세포의 미세구조 변화를 관찰하였다. STZ을 투여한 대조군 이자섬의 면역조직화학적 검사 결과 insulin에 양성반응을 보이는 세포가 거의 관찰되지 않았으나, 생진양혈탕가미방을 투여한 실험군의 $\beta$세포들은 대조군에 비하여 강한 양성반응을 보였다. 전자현미경 관찰에서, 대조군 생쥐의 $\beta$세포는 핵의 핵막이 정상군에 비하여 뚜렷하지 않았으며 세포질소기관의 발달도 미약하였다. 세포질내에 함유되어 있는 분비과립의 수가 현저히 감소하였으며 전자밀도가 높은 중심부분의 크기도 정상군에 비하여 현저히 작았다. 실험군의 $\beta$세포에서는 insulin분비소포의 수는 정상군에 비하여 다소 적게 관찰되었으나 대조준에 비해서는 증가하였으며, 전자밀도가 높은 중심부분의 크기도 대조군에 비하여 다소 증가하였다. 혈당은 대조군($365.33{\pm}91.01$, mg/dl)에비하여 실험군($179.56{\pm}73.36$, mg/dl)에서 유의성있게 감소하였다. 위와 같은 결과로 보아 생진양혈탕가미방이 STZ로 유발된 당뇨 생쥐의 치료에 효능이 있음을 확인할 수 있었다.

• 대한한방내과학회지
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• 제29권1호
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• pp.90-103
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• 2008

Objective : This study wascarried out to understand the effect of Sopyung-tang (SPT) on blood glucose & antioxidant enzyme activities in streptozotocin-induced diabetic rats. Methods : SD rats were separated into three groups, each with 20 rats. Except the normal group, the other two groups were intra-peritoneally injected with streptozotocin 6mg/kg. The experimental group was treated with SPT extract 500mgkg/day for 4 weeks. The normal and control groups were treated with saline 500mg/kg/day for 4 weeks. Changes of plasma glucose level and body weight were observed. After4 weeks, liver and kidney weight, antioxidant enzyme activities, and survival rate were observed with histological changes on liver, kidney and pancreas. Results : In the experimental group, body weight and survival rate increased, while plasma glucose level decreased significantly. Liver and kidney weight, XOD activity decreased in the experimental group compared to the control. GSH-px and CAT activities andinsulin-immunoreactive granules in ${\beta}-cells$ increased significantly in the experimental group compared to the control. Conclusions : This study shows that SPT might be effective for treatment of diabetes and its complications, as well as reduction of oxidative stress.