• Journal of Biosystems Engineering
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• 제29권6호
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• pp.515-520
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• 2004

A device far estimating the optimal artificial insemination time consisted of a computer, RS module, and six direct-reflex photo-sensors. Program was written in LabWindows CVI. In order to establish references for estimating optimal artificial insemination time, lying rate of the thirty three $Berkshire{\times}Hampshire crossbred sows (parity 5 to 6) was recorded from 2 days after moving into stalls to artificial insemination using a time lapse VCR and was measured every hour with one minute interval using the scan point sampling method. Twenty low hours in a day were divided into three comparing periods : 21:00 to 06:59, 07:00 to 13:59, and 14:00 to 20:59. If sum of the percentages of tying during a comparing period was less than the reference value, the starting hour of the comparing period was considered as onset of estrus and the optimal artificial insemination time was estimated at 20 to 24 hours after onset of estrus. The experimental device was evaluated with twenty five $Berkshire{\times}Hampshire$ crossbred sows (parity 2 to 7) and 23 of 25 sows $(92\%)$ were pregnant. This result suggests that the experimental device is excellent in performance for estimating optimal artificial insemination time.

• 한국가축번식학회지
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• 제8권2호
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• pp.87-91
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• 1984

This study was conducted to determine the optimum time and the insemination site of frozen in Cheju native cows, and 660 cows first service inseminated with rectovaginal methods by 3 skillful technicians as 3 districts near Cheju City and Sogwipo City areas obtained the following results. Optimum time for insemination was 16-20 hours after the onset of estrus and 80.6% of 67 conceived to first insemination. In the rectovaginal technique used widely at present conception rate for the palpation of ovaries showed 6.6% (index) higher than that of non-palpation. Conception rate of the insemination site; the intra-uterine horn, intra-uterus, just inside the cervix and depositing in mid-cervix were 62.5, 78.4, 74.6, and 80.9% respectively, however, there was no difference in conception rate between cervical and uterine deposition of forzon semen except for the intra-uterine horn.

• 한국수정란이식학회지
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• 제20권2호
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• pp.157-162
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• 2005

To find out the possible inefficiencies of artificial inseminators at rectovaginal insemination in cows, inseminators' skill were evaluated by controlling the semen thawing procedure adopted and by using the technique of dye deposition in the genital tract of slaughtered cows. This was followed by refreshment training for the inseminators. Thirty seven artificial insemination technicians regularly working in the government, cooperative and NGO (Non Government Organization) artificial insemination programmes at different places of Bangladesh were included in the study. Individual technicians were asked to thaw a semen straw and deposit dye in the genital tract of slaughtered cows following the procedures they would have adopted in their actual practices of insemination. The time and water temperature adopted by technicians were recorded and genital tract after sham artificial insemination was dissected to determine the site of dye deposition. Then, the inseminators took part in a three days intensive training program. The training program was ended up with the same tests for thawing frozen semen straw and dye deposition in the genital tract of slaughtered cows. At pre training evaluation, only $25\%\;and\;72\%\;(n=36)$ inseminators adopted co..ect thawing time and temperature, respectively. At post training evaluation, all inseminators thawed semen straws for proper time and temperature. At pretraining evaluation, $21(57\%),\;11 (30\%)\;and\;3(8\%)$ inseminators deposited dye at the body of uterus, in the vagina or in cervix, and into the horn of uterus, respectively. In $2(5\%)$ cases dye did not pass into the genital tract, instead back flowed through the space between the barrel of insemination gun and sheath. At post training evaluation, all inseminators successfully deposited dye in the body of uterus. Frequent evaluation of inseminators' skill and subsequent training would help improvement of the artificial insemination technicians' skill.

• 한국수정란이식학회지
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• 제12권2호
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• pp.161-169
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• 1997

This study was conducted to compare the insemination time of bovine oocytes and determine the effects of glucose(1.5 mM) on the development of bovine embryos at early cleavage stage. Oocytes were matured for 24 h, followed by exposure to sperm and cultured in modified Tyrode's media drops or with bovine oviduct epithelial cell monolayer prepared in TCM199(BOECM). Insemination time and culture system were varied in each experiment. In experiment 1, to investigate the developmental capacity of bovine embryos after different time of exposure to sperm, bovine ova and sperm were co-incubated for 18, 30 or 54 h, respectively. The development to blastocysts of 30 and 54 h insemination groups were significantly higher(P<0.05) than 18 h group, and in case of blastocysts of cleaved embryos, 30 h group were significantly higher(P<0.05) than other groups. In experiment 2, we investigated the effect of glucose on early bovine embryos. After 18 h insemination, in vitro fertilized oocytes were separated following 3 groups ; G+0, C+24 and C+48. Oocytes of G+0 group were cultured in glucose added Tyrode's medium after fertilization, oocytes in C+24 and C+48 groups were cultured in glucose free Tyrode's medium after fertilization. After 24 h culture, G+24 group was moved to glucose added medium. All oocytes of 3 groups were moved to BOECM after 48 h culture. The rates of cleavage and development to blastocysts in G+0 group were significantly lower than other groups. In experiment 3, we determined the effects of glucose exposure from 8 to 20 h after insemination on the cleavage and development of oocytes. The oocytes in glucose added group had high capacity of cleavage and further development. This study shows that in bovine oocytes, the optimal exposure to sperm is 30 h and glucose exposure to bovine one-cell embryos is detrimental to their first cleavage and further development in vitro but there has no evidence of detrimental effect of glucose(1.5 mM) exposure to bovine embryos over the two-cell stage in vitro.

• 한국가축번식학회지
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• 제17권2호
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• pp.121-125
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• 1993

Early development of bovine oocytes fertilized in vitro in the medium with caffeine and heparin was examined in different culture systems. When the oocytes were transferred into culture medium 8 h after insemination, 12%(7/60) of penetrated oocytes cleaved to 4-cell stage 24 h after insemination. The proportions of oocytes cleaved to 80to 16-cell stage 48 h after insemination had also a to be higher in oocytes transferred into culture medium 8 h (29%) than 16 h(10%) or 24 h(4%) after insemination. 52% of the 4-cell embryos developed to morula and blastocyst stages when they were co-cultured with oviductal epithelia, whereas only 5% of embryos cultured without the epithelial cells(P<0.001). In another experiment, embryos were co-cultured with ampulla, isthmus or utero-tubal junction of oviducts. There are no significant differences in the proportions of embryos developed to morula and blastocyst stage.

• Asian-Australasian Journal of Animal Sciences
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• 제4권2호
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• pp.151-156
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• 1991

Bovine in vitro fertilization experiment was carried out using ovary-derived follicular oocytes and frozen-thawed spermatozoa to determine the optimal preincubation time of spermatozoa and the insemination time for successful in vitro fertilization rate. The possibility of parthenogenetic cell division of unfertilized oocytes during culture without spermatozoa was also examined. There was no significant (p>0.05) difference in percent ratio of embryos developed to blastocyst stage between 0 and 3 h preincubation times of spermatozoa, showing a tendency to have higher percentage for 0 h of preincubation time. The 6 h insemination time seemed to be better for producing higher percentage of ova cleavage compared with those of 1 and 3 h treatments. Approximately 10% of unfertilized oocytes divided into 2 to 4-cell stage, and some of them cleaved to 5 up to 8-cells. The results obtained from this study suggested that 0 h of sperm preincubation time and 6 h of insemination time would be suitable for producing better in vitro fertilization rate of bovine oocytes. It is also likely that unfertilized bovine oocytes probably cleave to some cell stages with irregular divisions of the cells. On the one hand, considerable variation was also found in spermatozoa function among individual bulls.

• Clinical and Experimental Reproductive Medicine
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• 제17권2호
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• pp.137-144
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• 1990

Seventy-two patients initiated intrauterine insemination between March 1989 and March 1990. Indication for the intrauterine insemination included abnormal semen, poor cervical mucus, presents of sperm antibody, unexplained, poor postcoital test and endometriosis. During this time period, 216 inseminations were performed and resulted in twenty four pregnancies in the 72 patients receiving artificial insemination by homologous donor for an overall pregnancy rate of 33.3%. Where pergonal treatment was followed by insemination, a significantly greater pregnancy per cycle was achieved. It is therefore suggested that a trial if induction of multiple follicular development be performed on coulpes with male-related or unexplained infertility before inclusion in protocols for invasive procedures, such as IVF or GIFT.

• 한국동물학회지
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• 제39권2호
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• pp.173-181
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• 1996

The time-course process by which spermatozoa penetrates through the micropvle apparatus into the egg cytoplasm of rainbow trout, Oncorhvnchus mvkiss, was examined with transmission and scanning electron microscopy. In the unfertilized egg, the ess surface beneath the inner opening of the micropylar canal did not differ distinctly from the rest of the animal pole area. A spermatozoon attached to the micropvle opening 20 seconds after insemination. In the initial stases of penetration, the spermatozoon still within the micropvlar canal attached perpendicularly at its apical tip to the ess surface, then the sperm head was rapidly engulfed by the folded egg surface with its manly microvilli. A large fertilization cone with microvillus-free surface appeared on the esS surface sutra-rounding the penetrating spermatozoon. The head portion of the penetrating spermatozoon was completely wrapped by the ess surface with only the tail portion visible externally 30 seconds after insemination. The fertilization cone displayed the tail portion of the penetrating spermatozoon on the central portion of its surface 60 seconds after insemination. 150 seconds after insemination, breakdown of the cortical granules elevation were initiated at the animal pole, then completed at the vegetable pole area. The spermatozoon disappeared from the outer surface of the ess before the fertilization cone completely retracted 250 seconds after insemination. In result, the block to polvspermv to permit entry of a sin81e sperm is considered to be mechanical by the rnorpholoSical design of the micropvle and fertilization cone.

• 한국가축번식학회지
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• 제5권1호
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• pp.36-42
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• 1981

This experiment was carried out to determine the optimum interval between inseminations in artifical insemination of hens. Two hundred and forty hens of Hisex commercial stock at 25 weeks of age and 20 cocks of the Rhode Island Red at 40 weeks of age were used for the experiment, and a total of 6,784 eggs were obtained. The intervals between inseminations compared in this study were: 3 days (T1), 5 days (T2), and 7 days(T3). Mixed raw semen was inseminated and the semen does was 0.03ml per insemination per hen. The inseminations were conducted at 15:00 at each time. The total number of insemination performed was 9 for the T1, 6 for the T2 and 5 for the T3, and eggs were collected over a period of 31 days, 32 days and 35 dyas, respectively. The average egg production of the hens during the experiment was 85.9% and the average temperature during the experiment was around 30$^{\circ}C$. The average sperm count was 3.69 billion per ml. The results obtained in this experiment can be summarized as follows: The fertility over the entire experimental period bythe treatment was 91.7% for the T1, 84.4% for the T2, and 75.2% for the T3. The difference between T1 and T3 in fertility was significant at 5% level. The average fertility on the second, third and fourth day after the insemination in the T2 and T3 was maintained at a relatively high level, but it tended to decline rapidly from the fifth day after the insemination. The average fertility for one week after the last insemination was 88.8% for the T1, 88.8% for the T2 and 78.6% for the T3, and none of the differences among the treatments were statistically significant. On the basis of the results from this study, it is recommended to adjust the insemination intervals within the range from the 3 to 5 days in order to maintain a highest level of fertility in the hens at an early stage of egg production as in the case of the hens used in this experiment. An insemination interval of 3 days is recommended, especially at an initial stage of insemination. For the hens with a low fertility, shortening, of the insemination interval to 3 or 2 days is desirable.

• Journal of Animal Science and Technology
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• 제65권4호
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• pp.759-766
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• 2023

Visual estrus observation can only be confirmed at a rate of 50%-60%, which is lower than that obtained using a biosensor. Thus, the use of biosensors provides more opportunities for artificial insemination because it is easier to confirm estrus than by visual observation. This study determines the accuracy of estrus prediction using a ruminoreticular biosensor by analyzing ruminoreticular temperature during the estrus cycle and measuring changes in body activity. One hundred and twenty-five Hanwoo cows (64 with a ruminal biosensor in the test group and 61 without biosensors in the control group) were studied. Ruminoreticular temperatures and body activities were measured every 10 min. The first service of artificial insemination used gonadotropin-releasing hormone (GnRH)-based fixed-time artificial insemination protocol in the control and test groups. The test group received artificial insemination based on the estrus prediction made by the biosensor, and the control group received artificial insemination according to visual estrus observation. Before artificial insemination, the ruminoreticular temperature was maintained at an average of 38.95 ± 0.05℃ for 13 h (-21 to -9 h), 0.73℃ higher than the average temperature observed at -48 h (38.22 ± 0.06℃). The body activity, measured using an indwelling 3-axis accelerometer, averaged 1502.57 ± 27.35 for approximately 21 h from -4 to -24 h before artificial insemination, showing 203 indexes higher body activity than -48 hours (1299 ± 9.72). Therefore, using an information and communication techonology (ICT)-based biosensor is highly effective because it can reduce the reproductive cost of a farm by accurately detecting estrus and increasing the rate of estrus confirmation in cattle.