• Title/Summary/Keyword: insecticides.

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Molecular Cloning of the cDNA for Glutathione S-transferase Gene Homologue from the Mole Cricket, Gryllotalopa orientalis

  • Kim, Iksoo;Lee, Kwang-Sik;Kim, Jin-Won;Ryu, Kang-Sun;Sohn, Hung-Dae;Jin, Byung-Rae
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.04a
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    • pp.68-68
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    • 2003
  • The glutathione-S-transferases (GSTs) are enzymes responsible for the protection of cells from chemical toxicants and oxidative stress. In insects, GSTs have been particularly known to be implicated in the resistance to insecticides. In this study, a cDNA encoding the GST gene homologue was isolated from the cDNA library of the mole cricket, Gryllotalpa orientalis. (omitted)

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Studies on the Seasonal Occurrences and Several Insecticides for Control of the Mulberry Thrips, Pseudodendrothrips mori NIWA(Thysanoptera: Phloeothripidae) (뽕나무 총채벌레의 발생소장과 수종살충제 처리효과)

  • 백현준
    • Journal of Sericultural and Entomological Science
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    • v.18 no.2
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    • pp.61-64
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    • 1976
  • Experiments had been carried out in Suweon area from 1972 to 1973 to determine the seasonal fluctuation of Pseudodendrothrips mori NIWA using direct counts and to know the effects of chemicals for its control. 1. The fluctuation of mulberry thrips was especially influenced by precipitation and number of hours with sunshine in the meteological conditions. The peaks of fluctuation occurred several times but the highest peak occurred in late August. 2. The mulberry thrips was distributed in the range of the 4th to 16th leaves from the apex in a branch. The highest density leaves were 6th to 12th. 3. Orthene, Metasystox were the most effects on the mulberry thrips.

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Development of an ELISA for the Organophosphorus Insecticide Chlorpyrifos

  • Cho, Young Ae;Lee, Hye-Sung;Park, Eun-Yeong;Lee, Yong-Tae;Hammock, Bruce D.;Ahn, Ki-Chang;Lee, Jae-Koo
    • Bulletin of the Korean Chemical Society
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    • v.23 no.3
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    • pp.481-487
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    • 2002
  • A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide chlorpyrifos was developed. Four haptens for chlorpyrifos were synthesized and two of them were used as immunogens after coupling to keyhole limpet hemocyanin by two differe nt approaches. Rabbits were immunized with either of them and the sera were screened against 4 haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigencoated ELISA was developed, which shows an I50 of 160 ppb with a detection limit of 10 ppb. An antibody-coated ELISA was also developed, which shows an $I_{50}$ of 20 ppb with a detection limit of 0.1 ppb. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides except for insecticides chlorpyrifos-methyl and bromophos-ethyl, which makes these assays suitable for the selective detection of chlorpyrifos.

DEVELOPMENT OF SUPER WIDE SPREADER FOR DIRECT RICE SOWING IN WET PADDY FIELD

  • Kota Motobayashi;Yukio Yaji;Kim, Shoichi ura
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 1996.06c
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    • pp.999-1008
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    • 1996
  • The development and improvement of the originally designed direct rice sowing machine were carried out for the specific purpose of saving labor and increasing the efficiency of field operations in rice cultivation in the northern part of Japan. the prototype super wide spreader has a unique mechanism which propels rice seeds by compressed air and spreads them from the pivoting nozzle while the machine is in motion. The effective field capacity of sowing operation is about 6 ha/h. This spreader can also handle granular chemicals such as fertilizers, herbicides, pesticides and insecticides.

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Effects of Acute Oral Administration of Mancozeb on the Immune Function in Mice (Mancozeb의 급성노출이 마우스의 면역기능에 미치는 영향)

  • 정애희;표명윤
    • YAKHAK HOEJI
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    • v.48 no.1
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    • pp.41-46
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    • 2004
  • Mancozeb, a polymeric complex of zinc and manganese salts of ethylene bisthiocarbamate (EBDC), is used widely in agriculture as fungicides, insecticides, and herbicides. Mancozeb can be occupationally and environmentally exposed to human and has been reported to induce estrogenic activity, therein it is considered as an endocrine disrupter, We investigated the effects of acute exposure of Mancozeb on the immune function in mice. After single oral administration of Mancozeb to female ICR mice, the immunopathological parameters (body- and organ-weight, splenic cellularity hematological parameters), mitogen (Con A, PHA+IL-2, LPS)-induced splenocyte proliferation (SP) and splenic IgM plaque forming cell (PFC). WBC and splenic cellularity were decreased, but liver-, kidney-, and spleen-weight were increased when compared with control group. Splenic IgM PFC against SRBC was slightly lowered. Mitogen-induced proliferation of spleen cells from Mancozeb-treated mice was not signifcantly changed ex vivo, however, SP in vitro were significantly lowered in concentration dependent manner. These results indicate that Mancozeb could affect the immune function in mice.

Generation of Baculovirus Expression Vector Using Detective Autographa California Nuclear Polyhedrosis Virus Genome Maintained in Escherichia coli for $Occ^{+}$ Virus Production

  • Je, Yeon-Ho;Chang, Jin-Hee;Roh, Jong-Yul;Jin, Byung-Rae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.155-160
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    • 2001
  • We have generated a novel baculovirus genome which can be maintained in Escherichia coli that facilitates the rapid and efficient generation of recombinant baculovirus expression vectors. To make $Occ^{+}$ recombinant expression vectors, polyhedrin gene under the control of p10 promoter was inserted to bAcGOZA and this genome was designated bApGOZA. As in bAcGOZA, bApGOZA lacks a portion of the essential ORF1629 gene, but includes a mini-F replicon and selectable kanamycin-resistance marker, This occasion-producing activity of bApGOZA can be used very conveniently for its oral infectivity to insect larvae in mass production of foreign protein and insecticides.

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Screening of Insecticide to Control Rhophalosiphum nymphaeae for Alisma plantago as Second Crop in Paddy Field

  • Kwon, Byung-Sun;Lim, June-Taeg;Shin, Jeong-Sik
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.1
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    • pp.17-19
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    • 2003
  • The purpose of this study was to evaluate the effect of pesticides on insect pest control of Rhophalosiphum nymphaeae, growth characteristies and dry root yield from the cultivated after early maturing rice cropping. All pesticides treated had no effect on the growh period, flowering rate of Alisma plantago. The major pesticides were methomyl-Lf, 24.1 %, imidacloprid-Wp, 10%, carbosulfan-Wp, 20%, and methomyl-Wp, 45%. Dry yield of root were increased largely with imidacloprid-Wp, 10% (10g/20$\ell$), pesticide than the other pesticides and control. All pesticides were had no injury with recommended concentration. On the other hand all pesticides were slightly harmful in the double dosage level for the Alisma plantago.

Studies on Establishment of Oversown Pasture Seed I. Effects of coating materials and minerals on germination (겉뿌린 목초종자의 정착에 관한 연구 I. 각종 증량재 및 미량광물질의 종자피복이 발아에 미치는 영향)

  • 이효원;정병용;김희경
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.7 no.2
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    • pp.113-119
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    • 1987
  • This experiment was carried out in laboratory to find out the effect of coating materials, minerals and insecticides on germination of pasture seed. Seed coating was made by specially made machine and seed germination in petridishes was also determined from Sep. 1986 to May 1987. The results were summarized as follows; 1. Coating seed gave bad germination compared with the control. There were significant difference between treatment, coating materials and adhesives in white and red clover while Phleum pratense germination was improved by coating materials. 2. In mineral coating treatments, on Mg alone or Mg mixed treatment, germination was deepressed. Generally germination was lowed by mineral treatment, but the difference was small. 3. Uncoating treatment with insecticide was superior to coating treatment in terms of germination. Among the insecticide Ballen gave more serious effect to seed germination.

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Overall Assessment of Organochlorine Insecticide Residues in Korean Foods (한국식품(韓國食品)중 유기염소계(有機鹽素系) 잔류농약(殘溜農藥)에 관한 종합평가(綜合評價))

  • Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.14 no.1
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    • pp.82-93
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    • 1982
  • The use pattern and survey data on the residue levels of organochlorine insecticides in Korea were summarized. On the basis of available data on food consumption and residue levels, the daily intake of the pesticide chemicals by average Korean adults were calculated to give $13.77\;{\mu}g$ of total BHC, $2.45\;{\mu}g$ of total DDT and $4.06\;{\mu}g$ of heptachlor and its epoxide. These intake levels were compared with the acceptable daily intake proposed by FAO/WHO and values obtained in other countries. It was then proposed to undertake a total diet study on pesticide chemicals for the sake of food safety assurrance and reasonable regulation of pesticides for food production in this country.

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A Case Report of a First Sulfoximine Class of Insecticide, Sulfoxaflor Poisoning (설폭사플로르 살충제 중독 이후 발생한 저독성 보고 1례)

  • Oh, Jaehoon;Kang, Hyunggoo;Lim, Tae Ho;Lee, Sanghyun;Ahn, Chiwon
    • Journal of The Korean Society of Clinical Toxicology
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    • v.13 no.1
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    • pp.43-45
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    • 2015
  • Sulfoxaflor is the first insecticide belonging to the sulfoximine class and is efficient against sap-feeding insects that are resistant to other insecticides. Sulfoxaflor acts as a neurotoxin to the central nervous system of insects compared with very low toxicity to mammalian. We report on a case of a 67-year-old male who ingested insecticide and received conservative treatment for mild metabolic acidosis and gastrointestinal symptoms.

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