• International Journal of Industrial Entomology and Biomaterials
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• v.46 no.2
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• pp.34-40
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• 2023

As agricultural production increases due to population growth, agricultural by-products that are generated at all production stages increase commensurately. Black soldier fly (BSF) (Hermetia illucens) treatment has potential as an environmentally friendly process to combat the environmental pollution caused by agricultural by-products. This study examined the utilization of BSF larvae in the decomposition of the by-products of apples and mandarins, fruits commonly produced in South Korea. The BSF test larvae were fed apple pomace or mandarin waste, and the control larvae were fed calf feed. Larval weight and size were measured at 4-day intervals until larvae reached the pre-pupal stage. Larval development time, survival rate, and BSF fecundity rate were calculated for all three substrates. Waste reduction and bioconversion ratios were also calculated. The developmental time of larvae fed with apple pomace and mandarin waste was greater than that of the control larvae. The average weight of larvae fed with the fruit by-products was less than that of the control. There was no significant difference in the survival rate of BSF larvae or the fecundity rate of BSF between the substrates used in this study. BSF larvae decomposed 48.0% and 61.5% of apple pomace and mandarin waste, respectively. The bioconversion efficiency rates of BSF larvae fed with apple pomace and mandarin waste were 9.1% and 12.1%, respectively. These results indicated that decomposition of single fruit by-products by BSF larvae is an environmentally friendly and effective bioconversion process.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.2
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• pp.59-62
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• 2016

Many insects have gained increasing attention as an alternative protein for humans. Among those, the two-spotted cricket, Gryllus bimaculatus (Orthoptera: Gryllidae), was recently approved as a general food ingredient by the Korean Ministry of Food and Drug Safety. For industrial utilization of G. bimaculatus, mass rearing techniques and production system should be standardized first. In this study, we investigated the effects of five different feeds on the growth and development of G. bimaculatus. Feed is the one of the key factors that has considerable effects on rearing insects. With five different kinds of feed on $1^{st}$, $3^{rd}$, and $5^{th}$ instar nymphs, the change of survival rate, body weight were monitored up to eight wk after hatching. We concluded that 50% of soybean flour, 20% of corn powder, 10% of rice bran, 9% of milk serum, 10% of rice flour, 0.5% of microorganisms, and 0.5% of multivitamins and minerals (diet C) was the best mix for promoting growth and development of $3^{rd}$ instar nymphs compared to the control diet.

• Applied Biological Chemistry
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• v.34 no.2
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• pp.110-116
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• 1991

As the model compounds, citral and n-octanol which possess similar characteristics and structures of low molecular weight insect pheromones and $({\pm})-5-hydroxy-4-methyl-heptan-3-one$ which shows the aggregation pheromones activity of the rice weevil and the maize weevil were microencapsulated with low molecular weight polyethylene(LMPE) and urea-formaldehyde resin as wall materials. The core materials were microencapsulated as small particles in LMPE and urea-formaldehyde resin polymers and the microencapsulated polymers were white powders. And the polymer made from urea-formaldehyde resin was better than that from LMPE as wall material. The slow releasing effect and the releasing patten of the microencapsulated core materials were examined by solvent extraction method and headspace sampling method. Citral and n-octanol and $({\pm})-5-hydroxy-4-methyl-heptan-3-one$ were release more than 40 days and 15 days, respectively. The releasing pattern of urea-formaldehyde resin microcapsules showed rather smooth decrease than that of LMPE and was maintained at steady level longer.

• Journal of Environmental Science International
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• v.29 no.9
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• pp.939-941
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• 2020

This study evaluated the effect of four different feeds on the larval weight and survival rate of Protaetia brevitarsis seulensis (PBS) over five weeks. Four different types of feed (self-fermentation medium, RM medium, Samsung livestock medium, and bean-curd dregs medium) were given to third-instar larvae of PBS. Weight changes and survival rates of PBS larvae were measured weekly for five weeks. During the experimental period PBS larvae showed the highest growth in Samsung livestock medium, followed by bean-curd dregs medium, RM medium, and self-fermentation medium. Survival rate of PBS larvae were the lowest in bean-curd dregs medium, and then in the Samsung livestock medium. Using the Samsung livestock medium can shorten the delivery period of PBS larvae by approximately 40 days based on the larva shipped on average 75 days. Therefore, this study provides information and data on insect farming techniques useful for insect industrialization.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1753-1759
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• 2006

Paenibacillus sp. HY-8 isolated from the digestive tracts of the longicorn beetle, Moechotypa diphysis, produced an extracellular endoxylanase with a molecular weight of 20 kDa estimated by SDS-PAGE. The xylanase was purified to near electrophoretic homogeneity from the culture supernatant after ammonium sulfate precipitation, gel filtration, and ionexchange chromatography. The purified xylanase exhibited the highest activities at pH 6.0 and $50^{\circ}C$. The $K_m\;and\;V_{max}$ values were 7.2 mg/ml and 16.3 U/mg, respectively, for birchwood xylan as the substrate. Nucleotide sequence of the PCR-cloned gene was determined to have the open reading frame encoding a polypeptide of 212 amino acids. The N-terminal amino acid sequence and the nucleotide sequence analyses predicted that the precursor xylanase contained a signal peptide composed of 28 amino acids and a catalytically active 19.9-kDa peptide fragment. The deduced amino acid sequence shared extensive similarity with those of the glycoside hydrolase family 11 of xylanases from other bacteria. The predicted amino acid sequence contained two glutamate residues, previously identified as essential and conserved for active sites in other xylanases of the glycoside hydrolase family 11.

• International Journal of Industrial Entomology and Biomaterials
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• v.33 no.1
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• pp.15-23
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• 2016

One major constraint in the development and implementation of a successful and cost-effective area-wide integrated pest management (AW-IPM) programme with a SIT component for Bactrocera oleae (Diptera: Tephritidae) is the ability to produce a large number of high quality mass-reared individuals. The aim of this study was to develop a more efficient and practical egg collection system in an attempt to improve the mass-rearing of this species. The following basic parameters were examined: egg production per female, egg hatch, pupal recovery, pupal weight, adult emergence and percentage of fliers. Three different strains (Israel wild-type, France wild-type, and Greece laboratory) were tested and each strain was evaluated for six generations. Female flies of the Israel strain produced significantly more eggs per female than the other two strains, but egg hatch was significantly lower. Egg hatch of the France wild type and the Greece laboratory strain was similar. For all other parameters, there was no significant difference between strains; however, there was a significant generational effect for all parameters observed. As a result of this study, a protocol was developed for the mass-rearing of this species that included the use of large adult holding cages that could house up to 96,000 flies per cage. The newly developed method of egg collection using a flat wax panel as one of the sides of an adult holding cage proved to be cost-effective, efficient, making colony growth easier for industrial mass-rearing.

• International Journal of Industrial Entomology and Biomaterials
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• v.45 no.1
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• pp.22-28
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• 2022

Silkworms are very sensitive to changes in temperature and humidity, and unless it is a suitable temperature and humidity to grow, the productivity and quality of silkworms are greatly reduced. Therefore, it is very important to manage temperature and humidity for silkworm feeding facilities. In particular, it is essential to install heating facilities in Asian countries with distinct seasonal changes. During the feeding period, many farms manage the temperature and humidity of feeding facilities by installing and using electric heaters inside the facilities. However, it is very difficult to manage the room temperature stably by the heaters. In addition, unlike the temperature inside the facility, silkworms could undergo severe temperature changes as the inside of the rearing tray could not be warm enough. In this study, in order to improve the previous heating method, the new rearing method that directly heats the bottom of the rearing tray was developed. Compared to the previous room-heating system, the novel heater-installed tray (HIT) system significantly reduced the change in temperature during the experimental period. In addition, the number of days of silkworm growth up to harvest was shortened, which was effective in growth performance, and it was also found that silkworms grew more uniformly in HIT system than in previous system. Moreover, as the heater tubes were installed directly under the rearing tray, it quickly dried mulberry leaves and silkworm feces after feeding, and as a result, the environment in the tray was greatly improved with decrease the labor of breeder. In conclusion, these results suggest that the heater-installed rearing tray method greatly improves silkworm quality, increases weight of silkworms, and final profits compared to the previous room heating system with electric heaters.

• Journal of Sericultural and Entomological Science
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• v.39 no.2
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• pp.140-145
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• 1997

Silkworm fed on mulgerry leaves with 1000 times diluted Insegar(main component : Fenoxycarb, ethyl-2-(4-phenoxyphenoxy)ethylcarbmate) and its effects was investigated on increase of the 5th larval silkgland weight and silk quality. The oral application of fenoxycarb showed increase of the silkgland weight. The whole period of the silkgland weight falls on the 2nd instar treatment by 13%, the 3rd day of the 4th instar by 111%(3100 mg) and the whole period of the 5th instar treatment rather decreased it by 66%(497 mg), as compared to 1470mg of the control. Female and male both increased 15%(1779mg) and 12%(1554mg) of the silkgland weight with the 2nd instar treatment, as compared to 1548mg and 1391mg in control, respectively. 118%(3368mg) and 104%(2832mg) of increase in the silkgland weight were also showed with the 3rd day of the 4th instar treatment but 70%(463mg) and 61%(539mg) of decrease were shown with the 5th instar treatment. The maximum weight of the middle silkgland weight increased by 6%(1248mg) and 127%(2673mg) with the 2nd instar and with the 3rd day of the 4th instar, respectively but 67%(393mg) of decrease took place, as compared to 1175mg of the control. The maximum increase of the posterior silkgland weight with whole peried of the 2nd instar treatment increased 12%(419mg) and 75%(656mg) and 64%(135mg) of decrease was shown, as compared to 374mg of the control. The oral application of Insegar at the 2nd instar increased 26 m of cocoon length, 4.0 cg of the cocoon filament weight and 0.21d of the silk denier, as compared to 1147 m of the cocoon length, 38 cg of the cocoon filament, 2.99d of the silk denier, weight of cocoon filament and silk denier, respectively but denier variance narrowed to 0.33d, as compared to that of the control.

• Korean journal of applied entomology
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• v.58 no.3
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• pp.219-224
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• 2019

The super mealworm, Zophobas morio, is mainly used as food for pets, fish, reptiles, amphibians, birds, and as snacks. An experiment was conducted to investigate the effects of temperature, supplementary feeding, and spawning interval on super mealworm rearing. To efficiently rear this species, the insects were bred at a temperature of $27^{\circ}C$ or higher and reached a weight of over 0.6 g at 80 days after hatching. Supplementary feed (with high protein content: 10% of soybean meal and 10% of fish meal) enabled larvae to reach a weight of over 0.7 g at 80 days after hatching. Mass rearing of super mealworm requires increase in egg production (i.e., high yield) and uniformity of larvae. Adults were transferred to spawning bases every 5, 10, or 15 days (three treatments), which resulted in a total of 7,256, 5,439, 2,060 hatched larvae, respectively. It is possible to obtain more than 7,000 larvae by transferring the egg-laying frame to the spawning base at intervals of 5 days and with nine spawning operations; this procedure generates larvae weighing over 0.68 g each.

• Animal cells and systems
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• v.3 no.1
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• pp.41-46
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• 1999

An experiment was conducted among free-ranging red-winned blackbirds (Agelaius phoeniceus) that acquired illness-induced conditioned taste aversion (CTA) by consuming insect prey tainted with a dose of parathion up to 2.0 mg/kg consumer body weight. Birds quickly acquired CTA and avoided all four insect prey during a lengthy posttest without parathion. This experiment proved that organophosphate insecticide application in the field might decrease the food consumption of wild birds and may also affect the reproductive success of breeding birds. Thus, CTA acquired accidentally after eating insecticide contaminated insect prey appears to be one of the reasons for the decreasing number of breeding songbirds in North America.