• Korean Journal of Agricultural Science
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• v.45 no.1
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• pp.28-37
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• 2018

This study was done to develop environmental risk assessments and a biosafety guide for insect-resistant genetically modified rice at a LMO (Living Modified Organism) isolation field. In the LMO quarantine area of Kyungpook National University, the species diversities and population densities of non-target insects found on insect-resistant genetically modified rice (Bt-9) resistant to Cnaphalocrocis medinalis and on non-GM rices (Dongjin and Ilmi) were investigated. The Bt-9 event was therefore evaluated under field conditions to detect possible impacts on the above ground insects and spiders. The study compared transgenic rice and two non-GM reference rices, Ilmi and Dongjin, at Gunwi in Southern Korea in 2016. Each rice was grown on three $18m^2$ plots with a randomized block design. A total of 4,243 individuals from 43 families and 9 orders were collected from the LMO isolation field. In the three types of rice fields, a total of 1,467 individuals from the insect-resistant genetically modified rice (Bt-9), 1,423 individuals from the Ilmi, and 1,353 individuals from the Dongjin were collected, respectively. There was no difference between the population densities of the non-target insect pests, natural enemies and other insects on the insect-resistant genetically modified rice (Bt-9) and non-GM rices. These results provide the diversity and population density of non-target insects for an environment risk assessment survey on insect-resistant genetically modified rice and could be used as a guideline to make a biosafety assessment method for genetically modified crops.

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.263-266
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• 2011

Crop protection strategies in organic horticulture aim to prevent insect pest and plant disease problems through utilization of non-chemical based control means. In order to develop a model for management of plant diseases and insects in organic cucumber cultivation, we compared efficacies between chemical pesticide spraying system and biological control means in semi-forcing and retarding cucumber cultivation during 2005 and 2006. Conventional chemical spray program using various chemical pesticides was applied 5 - 10 days intervals, while two different non-chemical pesticide application programs using two formulated biopesticides Topseed$^{TM}$ and Q-fect$^{TM}$, Suncho$^{TM}$, and Sangsungje$^{TM}$ (biocontrol agents 1) and using egg-yolk and cooking oil(EYCO), Bordeaux mixture, Suncho$^{TM}$, and Sangsungje$^{TM}$ (biocontrol agents 2) were applied 5 - 7 days intervals during entire cucumber cultivation period. Efficacy of both biocontrol agents programs was effective to comparable to conventional chemical pesitice spray program to control plant diseases such as powdery mildew and downy mildew as well as insect pests such as aphids and thrips which are known as major threats in cucumber organic cultivation. In this study, we established and evaluated an effective and economic crop protection strategy using various biological resources can be used to control plant diseases and pests simultaneously in organic cucumber cultivation field.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.89-89
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• 2022

Soybean (Glycine max (L.) Merr.) is a representative food resource. To preserve the integrity of soybean, it is necessary to protect soybean yield and seed quality from threats of various pests and diseases. Riptortus pedestris is a well-known insect pest that causes the greatest loss of soybean yield in South Korea. This pest not only directly reduces yields but also causes disorders and diseases in plant growth. Unfortunately, no resistant soybean resources have been reported. Therefore, it is necessary to identify the distribution and movement of Riptortus pedestris at an early stage to reduce the damage caused by insect pests. Conventionally, the human eye has performed the diagnosis of agronomic traits related to pest outbreaks. However, due to human vision's subjectivity and impermanence, it is time-consuming, requires the assistance of specialists, and is labor-intensive. Therefore, the responses and behavior patterns of Riptortus pedestris to the scent of mixture R were visualized with a 3D model through the perspective of artificial intelligence. The movement patterns of Riptortus pedestris was analyzed by using time-series image data. In addition, classification was performed through visual analysis based on a deep learning model. In the object tracking, implemented using the YOLO series model, the path of the movement of pests shows a negative reaction to a mixture Rina video scene. As a result of 3D modeling using the x, y, and z-axis of the tracked objects, 80% of the subjects showed behavioral patterns consistent with the treatment of mixture R. In addition, these studies are being conducted in the soybean field and it will be possible to preserve the yield of soybeans through the application of a pest control platform to the early stage of soybeans.

• Korean journal of applied entomology
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• v.62 no.3
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• pp.193-205
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• 2023

Botanical insecticides derived from plant extracts exhibit repellent, antifeedant and enzyme-inhibiting activities against insect pests. Among such pests, phytophagous mites are major threats to horticultural crops. Botanical extracts derived from neem, chrysanthemum, and shrubby sophora are employed as field acaricides. These botanical extracts have low toxicities against natural enemies of the insect pests and, thus, are valuable in pest management. This review focuses on the potential for botanical extracts in the controls of mites, with comparisons of the spectrum of activity, the lethal dose and times and their mode of action. This information will enable better formulation of botanical extracts in integrated mite control.

• The Korean Journal of Pesticide Science
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• v.3 no.3
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• pp.60-67
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• 1999

Insecticidal activities of imidacloprid to sucking type insect pests, brown planthopper (BPH, Nilaparvata lugens) and peen peach aphid (GPA Myzus persicae), to chewing type insect pests, tobacco cut worm (TCW; Spodoptera litura) and beet armyworm (BAW, Spodoptera exigua) and to spiders, Pirata subpiraticus, Pachygnata clercki and Ummeliata insecticeps, as natural enemies were investigated by several bioassay methods. $LD_{50}$ of the chemical by topical treatment to BPH was 0.015 ${\mu}g/g$(48 hrs), while $LC_{50}s$ by leaf dipping and root zone treatment were 18.1 and 21.5 ppm, respectively. There was no difference in insecticidal activities between leaf dipping and root zone treatment. Imidacloprid also showed ovicidal effect of root zone treatment and its $LC_{50}$ was 6.8ppm $LD_{50}$ (48 hrs after treatment) of imidacloprid to GPA was $0.4{\mu}g/g$ in case of topical application and $LC_{50}s$ by leaf dipping and root zone treatment were 1.9 ppm and 13.7 ppm respectively. Leaf dipping was more effective than root zone treatment in GPA At topical application $LD_{50}$ (48 hrs after treatment) of imidacloprid to chewing type insect pests, TCW and BAW, were greater than 1,492 and $312{\mu}g/g$ and $LC_{50}s$ by leaf dipping method were 4,803 and Heater than 5,000ppm respectively. This means that imidacloprid has much less effect on chewing type insect pests, TCW and BAW. $LD_{50}$(48 hrs after treatment) of imidacloprid to wandering spiders, Pirata subpiraticus, Pachygnata clercki at topical application were greater dan $2941{\mu}g/g$ and greater than $2,976{\mu}g/g$ respectively and that to webbing spider, Ummeliata insecticeps, was 357 ${\mu}g/g$. Imidacloprid showed very low toxicity to the spiders and its selective toxicity ratios between spiders and BPH were greater than 19,600, greater than 19,800 and 23,800, respectively.

• Korean journal of applied entomology
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• v.47 no.2
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• pp.133-139
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• 2008

This study was performed to investigate the immature development period, fecundity, emergence rate and sex ratio of Sclerodermus harmandi against different host insects, Monochamus alternatus, M. saltuarius and Psacothea hilaris. Full grown larvae and pupae of host insects were provided with foods. The mean larval period of S. harmandi female was $29.2{\pm}0.93\;and\;25.1{\pm}0.47$ days in larvae and pupae of M. alternatus, $27.1{\pm}0.41\;and\;26.0{\pm}0.69$ days in M. saltuarius, and $26.3{\pm}0.38\;and\;31.2{\pm}0.24$ days in P. hilaris, respectively. S. hilaris adults were emerged at $12.9{\pm}0.2$ days in female and $11.9{\pm}0.2$ days in male after pupation when hosted M. alternatus pupa. Development period in male showed shorter one day than in female. Success rate of oviposition against different hosts was higher as 98.6 and 97.5% on full grown larva and pupa of M saltuarius. Emergence rate was higher as 90.1 and 87.3% on M. saltuarius larvae and pupae. Sex ratio of emerged S. harmandi adults was approximately 10:1 (Female : Male), females showed higher emergence rate than males. The period until first oviposition after emergence in S. harmandi female was the shortest in $4.6{\pm}0.1$ days on M. saltuarius pupa. When three females of S. harmandi were inoculated on M. saltuarius larva, the number of laid eggs was the highest $62.7{\pm}2.5$ per female.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.402-410
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• 2013

Insect pests damages are increasing on the field of Lycium chinense under environment- friendly management Thus, we was to monitor the occurrence of pests on organic L. chinense in Chungnam Cheongyang and, insecticidal effect of eco-friendly agricultural materials (EFAMs) were tested against major pests. When the buds come out, injury by L. chinense was very high causing the high population density of Myzus persicae in late May and early June, and the injury by Lema decempunctata was increased from mid-June. Otherwise, injuries by Eriophys macrodonis, Henosepilachna vigintioctomaculata and Hedma spp. were continued throughout the growing season. From the fruit held in late July, Hemipteran insects including Plautia stali and Halyomorpba balys were sucking the fruit, and their injury to L. chinense were gradually increased. For the eco-friendly environmental control of M. persicae, L. decempunctata and E. macrodonis, EFAMs containing Sophora flavescens, Melia azedarach (Chinaberry), Chenopodium ambrosioides (Mexican tea), Quassia amara (Amargo), Stemona sessilifolia, Sophora seeds and Nepeta cataria (Catmint), were selected and used to control the insect pests. Control effects of the mixed extract with S. flavescens and Sophora seeds against M. persicae and L. decempunctata were higher than any other EFAM as 84.0% and 86.6%, respectively. In case of E. macrodonis, its control effect was good with the mixed extract with S. flavescens, C. ambrosioides and M. azedarach.

• Journal of Ecology and Environment
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• v.36 no.2
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• pp.125-129
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• 2013

The study on the major insect pests community of Hibiscus syriacus. There were three orders, seven families, and thirteen species of insects harmful to H. syriacus. When the harmful insects eating H. syriacus were classified according to which part of the flower they were eating, insects eating leaves, which considered of nine species four families and one order, accounted for 69.23 %. Insects sucking juice from leaves and stems comprised 15.38 %, with two species two families and two orders, while insects eating seeds comprised 15.38 %, with two species two families and two orders. Aphis gossypii appeared once per H. syriacus from the fourth week of April to the second week of June, appearing the most at a rate of 69.05 per H. syriacus during the first week of May. Dichocrocis surusalis appeared at a rate of 0.05 per H. syriacus during the third week of April and regularly appeared from the first week of July to the second week of September. Nortacha derogata appeared at a rate of 0.1 per tree during the first week of May, after which it continuously appeared until the third week of October. Anomis privata appeared at a rate of 0.05 per tree during the first week of May and continuously occurred from the fourth week of June to the fourth week of October. Its occurrence was most frequent at 2.30 per tree during the third week of September.

• Korean journal of applied entomology
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• v.33 no.4
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• pp.281-285
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• 1994

This study was conducted to establish a nce insect pest control system, ~ es.ys tematic application of insecticides using carbohran and buproiezin, in Korea. The effects oi various dates of application and rates of buprofezin (25% WP) after carbofuran (3G) soil incorpombon in late May on the pop~~lation densities of the brown planthoppa (BPH) immigrating in July were investigated Appropriate application tune of buprofezin for the BPH that had evaded insecticidal effect of 5011 incorporated carbofuran was late July-early August. Application rate of buprofezln at 7.0g a.i/lOa was enough to suppress the BPH density. Buprofezin treatment after carbofumn soil incorporation could also suppress the whlte backed planthopper population but did not affect the densities oi the paddy rice spiders. Considering the charactenstics of occurring patterns of the nce insect pests in Korea. buprofezin treatment m late July or early August after carbofuran soil incorporation in late May can be a useful application system of ir~sectic~deins controlling early season Insect pests and migmtoly planthoppers on rice.