• Journal of Applied Biological Chemistry
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• 제54권4호
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• pp.290-295
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• 2011

본 연구는 산약(Disocorea batatas)을 대상으로 토양 및 관개용수의 질 평가, 화학비료 및 퇴비의 사용실태 그리고 잡초 및 병해충 실태에 대한 현장 및 설문조사를 통하여 생산단계에서 good agriculture practices (GAP) 기반조성에 유용한 데이터를 제시하고자 하였다. 산약 재배지 토양과 관개용수 중 카드뮴, 납, 구리 및 아연 모두 자연함유량 수준으로 조사되었다. 산약재배지 관개용수 가운데 총질소는 대부분의 조사지점에서 기준치를 초과하였으나 총인은 기준치 이하로 나타났다. 산약 재배지에 화학비료와 퇴비가 복합적으로 과량 투입되고 있음이 현장 설문조사에서 나타나 최적의 양분종합관리 프로그램의 도입이 필요한 것으로 나타났다. 산약 재배지에서 우점 잡초는 바랭이(Digitaria sanguinalis), 쇠비름(Portulaca oleracea) 그리고 깨풀(Acalypha australis)로 나타났으며, 제초제 처리횟수는 1년에 2회 처리가 가장 많았고, 일부 농가에서는 4회까지 처리하는 경우도 있었다. 또한 산약 재배지에서 병해는 탄저병과 잎마름병이 주로 발생하였고, 해충으로는 응애가 주로 출현하는 것으로 조사되었다. 병해충 방제 목적으로 살충제나 살균제를 연 4~5회 처리하고 있었다. 산약의 안전성과 지속적인 농업생산성을 유지하기 위해서는 화학비료와 농약의 저투입이 필요하며, 궁극적으로는 유기농업에 의한 우수 산약의 재배기술 개발이 이루어져야 할 것이다.

• 한국환경과학회지
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• 제19권2호
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• pp.185-196
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• 2010

The distribution of benthic macroinvertebrates in Daecheon stream, an urban stream of Busan, was investigated to analyze the community structure of benthic macroinvertebrates. The collection was performed monthly at five(A~E) sites divided into three parts, upper, middle and lower, of stream from January to November, 2004. In physicochemical analysis of environmental factors, water quality parameters such as BOD, COD, conductivity and ABS were relatively increased in sites B and C. Whereas sites A, D and E showed little variations with good water quality parameters. However, water quality parameters in all surveyed sites showed gradual decrease with time toward improvement of water quality. A total of 8,226 individuals including 4 phyla, 6 classes, 9 orders, 302 families and 44 species were identified from five sites. The most dominant group was insect(class Insecta), and order Ephemeroptera and Diptera among insect was the largest member in species(30.6%) and individuals(75.0%) of benthic macroinvertebrates, respectively. The primary dominant species were Gammarus sp. and Ephemera strigata in site A, whereas Chironomus sp. and Brenchiura sowerbyi were dominated commonly in the other sites. In community analysis of benthic macroinvertebrates of Daecheon stream diversity index showed relatively low values, whereas dominance index was significantly high. Diversity index was the highest in site A, whereas the dominance index was the highest in site B. However, diversity index showed gradual increase with time showing adverse mode in dominance index. From these results, it can be suggested that long-term ecological monitoring of benthic macroinvertebrate fauna is needed for sustainable management of Daecheon stream.

• International Journal of Industrial Entomology and Biomaterials
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• 제4권2호
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• pp.83-91
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• 2002

The protection of silkworm and its host plants from various kinds of pests parasite and predator is a chronic problem in sericulture. Silkworms and its primary food plants are heavily damaged by large number of pest. The major pests of primary tasar food plants (Terminalia arjuna and Terminalia tomentosa) are the gall insect (Trioza fletcheri minor). Various species of aphids (Eutrichosiphum sp.) have been recorded to damage oak tasar food plants whereas muga silkworm host plants (Machilus bombycina and Litsaea polyantha) are generally attacked by stem bores (Zeuzera multistrigata). Castor (Ricinus communis) is one of the primary host plant of eri silkworm and extensive damage is caused by the castor white fly (Trialeurodes ricini). Insects pests are major enemies of silkworms. Parasites (Blepharipa zebina, Exorista bombycis, Apateles glomeratus), predators (Canthecona furcellata, Sycanus collaris, Hierodulla bipapilla), wasps (Vespa orientalix) and ants (Oecophylla smargdina) continues to cause damage to silk industry. It is estimated that the losses due to parasites and predators are to an extent of 15-20 percent and varies from crop to crop. The complexities in the behaviour and life cycle of pest population existing in semi ecosystem warrant a special attention for their effective management specially in changing scenario for our modern sericulture. Though use of synthetic insecticides has provided us with effective control of almost all major pests and predators, yet their undesirable side effects limit their continued use. Biological control is one of the most important method which can be used to control the pests, parasites and predators population in sericulture. Various potential parasitoids, which can be utilized as an agent of biological control in sericulture have been screened. The natural enemies of the uzi fly (E. bombycis and B. zebina ) are already present in the nature. Nesolynx thymus, Trichria sp., Splangia endius, Dirhinus sp., Trichopria sp., Trichomalopsis apanteloctena and Pediobius sp. are the major parasitoids effective against uzi fly pupa. The scelionid Psix striaticeps and Trissolcus sp. are the Potential egg Parasitoids against stink bug (Canthecona furcellata). Various other native natural potential parasitoids have been screened and suitable strategies have been developed to check the population of pest insect in sericulture.

• 대한수의학회지
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• 제39권3호
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• pp.602-608
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• 1999

Two-day old house fly adults were exposed to six insect growth regulators, flufenoxuron, teflubenzuron, triflumuron, diflubenzuron, methoxyfenozide, tebufenozide, as a feed additive (milk+5% sugar+chemical) in the laboratory for 6 days. The number of eggs deposited by the exposed-adults, viability of the eggs, and $F_1$ larval development were checked. All the IGRs tested were found to have no adverse effect on the reproduction of house fly, except methoxyfenozide (210ppm). The most effective inhibitor to egg hatch was flufenoxuron, followed by teflubenzuron, triflumuron, and diflubenzuron. Exposure to flufenoxuron (over 5ppm), teflubenzuron (over 25ppm), triflumuron (over 125ppm), and diflubenzuron (over 125ppm) reduced egg hatchability to 0 to 1.3%, but lower concentrations of these IGRs were less effective (6.3 to 46.3% egg hatchability). Almost all the larvae emerged from eggs deposited by the adults exposed to diflubenzuron (62.5ppm) and teflubenzuron (12.5ppm) failed to develop into pupae, causing total mortalities of 98% and 100%, respectively. However, two IGRs, methoxyfenozide and tebufenozide, did not inhibit egg hatch and $F_1$ larval development, except methoxyfenozide (210ppm) treatment These results suggest that these 4 IGRs may be used in the development of autosterilization system for house fly control. However, further work is required to develop delivery systems capable of transferring an effective dose to the fly under field conditions.

• 한국환경과학회지
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• 제16권3호
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• pp.277-285
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• 2007

The purpose of this study is to analyze seasonal distribution of insects, birds, fishes, and benthos for Gongji stream in Chuncheon and to explore some strategies towards creating a desirable close-to-nature stream. The number of species occurred in the study stream was 23 for butterflies and 149 for other insects, 23 for birds, 15 for fishes, and 47 for benthos. The study stream was dominated by Artogenia rapae, Polygonia caureum, Cyntia cardui for butterflies, and Passer montanus, Anas crecca, Anas platyrhynchos for birds. The dominant species were Rhynchocypris oxycephalus, Zacco plutypus, Carassius auratus for fishes, and Limnodrilus sp. 2, Chiranumus sp. 2, Hydropsyche KUb for benthos. Attracting insect species of higher density requires planting of host plant species and stabilization of plant coverage against disturbances. To enhance species diversify and density of birds, it is desirable to provide diverse habitats including preservation of downstream wetland and natural vegetation, creation of sand bars and islands with sand and gravel, and restriction or traffic passing and parking. Restoring fish and benthos habitats needs improvement of water quality, layout of gravels and stones on sand-dominant streambed, and use of close-to-nature revetment techniques.

• Entomological Research
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• 제48권5호
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• pp.354-361
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• 2018

Plant essential oils (EOs) exhibit an array of biological activities against insect pests. However, their negative influences on the pheromonal activity of azuki bean beetle (ABB), Callosobruchus chinensis L. have not received research attentions. ABB is a field-to-storage pest of legumes, and its female produces the sex pheromone known as homofarnesal with two isomeric components: 2E- and 2Z-homofarnesal, (2E,6E)-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals and (2Z,6E)-7-ethyl-3,11-dimethyl-2,6,10-dodecatrienals. We evaluated the effects of three EOs and their two major components on the attractiveness of male ABBs to synthetic homofarnesal (2E-:2Z-homofarnesal = 6:4) using Y-tube olfactometry in laboratory and rocket traps in the semi-open polyhouse. Y-tube olfactometry showed the significant negative effect of EOs of Illicium verum, Croton anisatum at 10 and 100 ng, and Gaultheria fragrantissima at 100 ng against homofarnesal (100 ng) in attracting male ABBs. Similarly trans-anethole (at 10 and 100 ng) and methyl salicylate (at 100 ng) also ascertained significant negative effect against homofarnesal (100 ng) in Y-tube olfactometry. When 10 mg of each of trans-anethole and methyl salicylate was released at the downstream of 30 mg homofarnesal lure in rocket traps, highly significant effect was achieved against attractiveness of homofarnesal to ABB males. This study ascertained significant level of negative effect of the tested EOs and their major components to homofarnesal, tracing out a new opportunity of integrating them in ABB management programs both in field and storage.

• 한국응용곤충학회지
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• 제61권1호
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• pp.15-28
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• 2022

신경펩타이드(Neuropeptide)는 신경세포에서 분비되는 단백질성 물질로, 곤충 호르몬에서 가장 큰 그룹으로 차지한다. 이들은 곤충의 전 생육단계에 걸쳐 지방체의 항상성, 섭식, 소화, 배설, 순환, 번식, 탈피/변태 등 다양한 생리적 기능과 행동을 조절하는데 관여하고 있다. 신경호르몬 일종인 PRXamide (NH₂) 펩타이드 계열 호르몬은 카르복실기 끝에 PRX (X, 다양한 아미노산)라는 공통의 아미노산 서열이 특징적으로 존재하고 있으며, 곤충 전반에 걸쳐 발견된다. 곤충에서PRX 신경호르몬은 다양한 생물학적 기능에 관련하고 있는데 호르몬구조와 기능에 따라 크게 3가지로 분류한다. Pyrokinin (PK)계열의 호르몬은 페로몬 생합성 활성화 신경펩타이드(pheromone biosynthesis activating neuropeptide, PBAN) 및 휴면 호르몬(diapause hormone, DH)이 속하며, 카파(CAPA) 펩타이드 호르몬, 그리고 탈피촉진 호르몬(ecdysis trigging hormone, ETH)이 여기에 속한다. PK 계열의 PBAN 호르몬은 지금으로부터 약 30년전 나방에서 처음 밝혀졌으며, 성페로몬 생합성을 자극하는 신경호르몬으로 확인되었다. 그 이후, PBAN의 연구는 절지동물 전반에 걸쳐 다양한 연구자들에 의하여 광범위하게 이루어졌다. 본 종설은 PBAN의 유전자 구조와 발현, PBAN에 의한 세포신호 전달과 성페로몬 생합성에 관련된 생리적 기작, 그리고 신경호르몬과 PBAN을 이용한 새로운 해충 방제법 개발의 가능성과 예를 소개한다.

• 한국응용곤충학회지
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• 제61권1호
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• pp.51-62
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• 2022

많은 해충이 과수의 다양한 조직을 가해하여 국내 과수산업을 위협하고 있다. 경제적으로 중요한 과수 해충의 방제는 일반적으로 화학 살충제에 의존하고 있다. 환경과 인류 건강에 미치는 살충제의 영향에 대한 우려가 증가함에 따라 환경 친화적인 해충방제 전략이 시급하게 필요한 실정이다. 과수 해충의 페로몬은 교미교란이나 대량포획과 같이 환경적으로 안전한 방제 체계를 끌어낼 수 있다. 본 종설은 과수 해충 51종에서 알려진 페로몬의 유형과 성분을 요약한다. 장님노린재과, 진딧물과, 깍지벌레과, 가루깍지벌레과, 풍뎅이과, 혹파리과 및 씨살이좀벌과에 속하는 14종과 나비목의 26종은 암컷이 성페로몬을 생산하여 수컷을 유인한다. 반대로 호리허리노린재과, 노린재과, 총채벌레과 및 하늘소과에 속하는 11종은 수컷이 집합페로몬을 생산하여 암컷과 수컷 모두를 유인한다. 향후 과수 해충 관리에서 페로몬과 다른 신호화학물질의 사용을 확대하기 위해서는 새로운 해충의 페로몬, 주요 해충의 카이로몬, 장기간 페로몬 방출용 교미교란제 및 대량포획을 위한 트랩 디자인과 트랩 설치에 관한 연구가 필요할 것으로 보인다.

• 한국응용곤충학회지
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• 제62권3호
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• pp.193-205
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• 2023

식물추출물은 작물 해충에 대해 기피, 섭식저해, 해충 효소활성억제 활성을 가지고 있어 해충 방제제로 활용되고 있다. 응애는 원예작물에 심각한 피해를 주고 있고, 실제 포장에서 방제 방법도 화학적 살비제를 이용하고 있지만 효과적이지 않다. 포장에서 응애 방제가 어려운 이유는 짧은 세대수, 많은 산란수와 번식력 등으로 인해 약제저항성 개체가 증가하는 것이 원인이다. 친환경자재로 님, 멀구슬, 제충국, 고삼 추출물을 함유한 제품들이 개발되고 포장에서 살비제로 활용되고 있다. 이들 추출물은 천적에 대한 낮은 독성으로 식물추출물과 천적을 동시에 사용하여 응애의 종합 방제에 활용될 수 있는 장점이 있다. 많은 이들 식물추출물의 해충 방제에 대한 리뷰가 있지만, 포장에서 효율적인 이들 추출물의 종합적인 측면에서 활용이나, 응애별 살충 스펙트럼, 반수 치사농도와 치사시간, 살충기작별로 응애 생활사에 영향을 미치는 종합적인 리뷰는 제한적이다. 본 리뷰는 이들 식물추출물을 포장에서 효율적으로 활용 가능한 식물추출물별 사용 가능 시기, 방법, 응애 생활사, 천적 활용 등의 종합적인 응애 방제를 목표로 하였다.

• BMB Reports
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• 제39권4호
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• pp.432-440
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• 2006

A tuber lectin from Arisaema jacquemontii Blume belonging to family Araceae was purified by employing a single step affinity chromatography using column of asialofetuin-linked amino activated silica beads and the bound lectin was eluted with 100 mM glycine-HCl buffer pH 2.5. The purified A. jacquemontii lectin (AJL) showed a single protein band with an apparent molecular mass of 13.4 kDa when submitted to SDS-polyacrylamide gel electrophoresis under reducing as well as non-reducing conditions. The native molecular mass of AJL determined by gel filtration on a Biogel P-200 column was 52 kDa and its carbohydrate content was estimated to be 3.40%. Thus AJL is a tetrameric glycoprotein. The purified lectin agglutinated erythrocytes from rabbit but not from human. Its activity was not inhibited by any of the mono- and disaccharides tested except N-acetyl-D-lactosamine having minimal inhibitory sugar concentration (MIC) 25 mM. Among the glycoproteins tested only asialofetuin was found to be inhibitory (MIC $125\;{\mu}g/mL$). A single band was obtained in native PAGE at pH 4.5 while PAGE at pH 8.3 showed two bands. Isoelectric focusing of AJL gave multiple bands in the pI range of 4.6-5.5. When incorporated in artificial diet AJL significantly affected the development of Bactrocera cucurbitae (Coquillett) larvae indicating the possibility of using this lectin in a biotechnological strategy for insect management of cucurbits. Larvae fed on artificial diet containing sub-lethal dose of AJL showed a significant decrease in acid phosphatase and alkaline phosphatase activity while esterase activity markedly increased as compared to larvae fed on diet without lectin. Out of various human cancer cell lines employed in sulphorhodamine B (SRB) assay, this lectin was found to have appreciable inhibitory effect on the in vitro proliferation of HCT-15, HOP-62, SW-620, HT-29, IMR-32, SKOV-3, Colo-205, PC-3, HEP-2 and A-549 cancer cell lines by 82, 77, 73, 70, 41, 41, 37, 29, 21 and 21% respectively.