• Journal of Applied Biological Chemistry
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• 제56권3호
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• pp.183-185
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• 2013

Two styrylpyranones (1 and 2) were isolated from the $CH_2Cl_2$-soluble fraction of Phellinus linteus and their structures were established by extensive Nuclear magnetic resonance (NMR) spectral data as inoscavin E and C. Both compounds showed significant prolyl endopeptidase inhibitory activity with $IC_{50}$ values of $4.26{\pm}0.14$ and $4.08{\pm}0.04{\mu}M$ and $K_i$ values of $1.50{\pm}0.02$ and $1.43{\pm}0.03{\mu}M$, respectively. They also exhibited antioxidant capacities against the ABTS radical system with $EC_{50}$ values of $6.47{\pm}0.05$ and $7.64{\pm}0.06{\mu}M$, respectively.

• Mycobiology
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• 제42권2호
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• pp.185-188
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• 2014

We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.

• 대한화장품학회지
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• 제31권4호
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• pp.349-357
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• 2005

미생물, 버섯류, 약용식물 등 천연물 대사산물로부터 미백, 주름개선용 화장품 신소재로 활용 가능한 항산화 신소재를 탐색하였다. 그 결과 Eupenicillium shearii 균주 배양액으로부터 4개의 phenol계 항산화 활성물질을 순수하게 분리하고 화학구조를 결정하여 melanocin A-D로 명명하였다. Melanocin $A{\sim}D$ 화합물의 DPPH 라디칼 소거활성은 $EC_{50}21{\sim}94{\mu}M$, superoxide 소거활성은 $EC_{50}\;7{\sim}84{\mu}M$로 arbutin 및 BHA보다 우수한 활성을 나타냈으며, 주름개선 효과도 매우 우수하였다. 기와층버섯(Inonotus xeranticus) 자실체 추출물로부터 강력한 항산화 활성물질인 히스피딘계 신물질을 얻어 inoscavin A로 명명하였다. Inoscavin A 화합물의 항산화 활성을 측정한 결과, superoxide radical 소거활성은 $EC_{50}\;0.03{\mu}g/mL$, rat의 간 microsome의 지질과산화 억제활성 $EC_{50}$은 $0.3{\mu}g/mL$로서 ${\alpha}-tocopherol$의 $1.5{\mu}g/mL$, BHA의 $4.9{\mu}g/mL$ 보다 우수하였다. Streptomyces nitrosporeus 균주 배양액으로부터 신규 지질과산화 억제 활성물질을 benzastatin $A{\sim}G$를 분리하였다. Benzastatin $A{\sim}G$의 지질과산화 억제활성 $EC_{50}$는 $3{\sim}30{\mu}M$로 매우 우수하였다. Penicillium sp. 균주로부터 미백활성물질을 탐색하여 cyclopentene계 화합물인 terrein을 얻었다. Terrein은 kojic acid 보다 약 10배 이상 강력한 미백활성을 나타낸 반면, $100{\mu}M$ 농도에서도 비교적 안전한 화합물로 확인되었으며, 작용기전은 MITF 단백질을 조절함으로 활성을 나타내는 것으로 확인되었다.

• 한국버섯학회지
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• 제18권1호
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• pp.29-36
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• 2020

P. linteus(품종명: 고려상황, PLKS)와 P. baumii (장수상황, PBJS)를 동일한 조건하에서 인공재배하여 얻어진 자실체를 70% 메탄올, 60% 에탄올, 열수추출하여 폴리페놀과 플라보노이드 측정 시 함량은 60%에탄올 추출물에서 가장 높게 나타났다. 60%에탄올을 용매로 PLKS와 PBJS의 자실체를 추출하여 농도별로 DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2'-azino-bis (3-ethylbisthiazoline-6-sulfonic acid) 라디칼 소거능을 측정한 결과 PLKS와 PBJS는 거의 유사한 항산화 활성을 보였으며 자실체가 균사체보다 2-3배 이상 높은 항산화 활성이 측정되었으나 FRAP (ferric reducing antioxidant power)분석에서는 자실체와 균사체 간에 큰 차이가 없는 것을 확인하였다. PLKS와 PBJS의 자실체 ethyl acetate 분획물을 HPLC 분석한 결과 styrylpyrone 계 폴리페놀 성분인 davallialactone, hispidin, hypholomine B, inoscavin A를 검출할 수 있었으나 균사체에서는 검출되지 않아 자실체 특이적인 화합물인 것을 확인하였다.

• 한국균학회소식:학술대회논문집
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• 한국균학회 2016년도 춘계학술대회 및 임시총회
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• pp.41-41
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• 2016

Influenza viruses are RNA viruses that belong to the Orthomyxoviridae family, and those can be divided into three types; A, B, and C, which based on the differences of the inner nucleoproteins and genomic structures. All three genera differ in their genomic structure and nucleoprotein content, they are further classified into various serotypes based on the two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). These glycoproteins play crucial roles in viral infection and replication. Hemagglutinin mediates binding of virions to sialic acid receptors on the surfaces of target cells at the initial stage of infection. Neuraminidase cleaves the glycosidic bonds of sialic acids from the viral and cell surfaces to release the mature virions from infected cells, after viral replication. Because NA plays an important role in the viral life cycle, it is considered an attractive therapeutic target for the treatment of influenza. The methanolic extracts of Phellinus baumii and Phellinus igniarius exhibited significant activity in the neuraminidase inhibition assay. Polyphenolic compounds were isolated from the methanolic extracts. The structures of these compounds were determined to be hispidin, hypholomine B, inoscavin A, davallialactone, phelligridin D, phelligridin E, and phelligridin G by spectroscopic methods. Compounds inhibited the H1N1 neuraminidase activity in a dose-dependent manner with $IC_{50}$ values of 50.9, 22.9, 20.0, 14.2, 8.8, 8.1 and $8.0{\mu}M$, respectively. Moreover, these compounds showed anti-influenza activity in the viral cytopathic effect (CPE) reduction assay using MDCK cells. These results suggests that the polyphenols from P. baumii and P. igniarius are promising candidates for prevention and therapeutic strategies against viral infection.