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Lipoxygenase Inhibitory Activity of Korean Indigenous Mushroom Extracts and Isolation of an Active Compound from Phellinus baumii

  • Lee, Seung Woong (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Song, Ja-Gyeong (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Hwang, Byung Soon (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Kim, Dae-Won (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, Yoon-Ju (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Woo, E-Eum (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Kim, Ji-Yul (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
  • Received : 2014.05.14
  • Accepted : 2014.05.20
  • Published : 2014.06.30

Abstract

We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.

Keywords

References

  1. Wang XM, Zhang J, Wu LH, Zhao YL, Li T, Li JQ, Wang YZ, Liu HG. A mini-review of chemical composition and nutritional value of edible wild-grown mushroom from China. Food Chem 2014;151:279-85. https://doi.org/10.1016/j.foodchem.2013.11.062
  2. Zjawiony JK. Biologically active compounds from Aphyllophorales (polypore) fungi. J Nat Prod 2004;67:300-10. https://doi.org/10.1021/np030372w
  3. Lee IK, Yun BS. Styrylpyrone-class compounds from medicinal fungi Phellinus and Inonotus spp., and their medicinal importance. J Antibiot (Tokyo) 2011;64:349-59. https://doi.org/10.1038/ja.2011.2
  4. Kim SE, Hwang BS, Song JG, Lee SW, Lee IK, Yun BS. New bioactive compounds from Korean native mushrooms. Mycobiology 2013;41:171-6. https://doi.org/10.5941/MYCO.2013.41.4.171
  5. Dobrian AD, Lieb DC, Cole BK, Taylor-Fishwick DA, Chakrabarti SK, Nadler JL. Functional and pathological roles of the 12- and 15-lipoxygenases. Prog Lipid Res 2011;50:115-31. https://doi.org/10.1016/j.plipres.2010.10.005
  6. Duroudier NP, Tulah AS, Sayers I. Leukotriene pathway genetics and pharmacogenetics in allergy. Allergy 2009;64:823-39. https://doi.org/10.1111/j.1398-9995.2009.02015.x
  7. Berger W, De Chandt MT, Cairns CB. Zileuton: clinical implications of 5-lipoxygenase inhibition in severe airway disease. Int J Clin Pract 2007;61:663-76. https://doi.org/10.1111/j.1742-1241.2007.01320.x
  8. Devi NS, Doble M. Leukotriene c4 synthase: upcoming drug target for inflammation. Curr Drug Targets 2012;13:1107-18.
  9. Chan KY, Mohamad K, Ooi AJ, Imiyabir Z, Chung LY. Bioactivity-guided fractionation of the lipoxygenase and cyclooxygenase inhibiting constituents from Chisocheton polyandrus Merr. Fitoterapia 2012;83:961-7. https://doi.org/10.1016/j.fitote.2012.04.018
  10. Ribeiro D, Freitas M, Tomé SM, Silva AM, Porto G, Cabrita EJ, Marques MM, Fernandes E. Inhibition of LOX by flavonoids: a structure-activity relationship study. Eur J Med Chem 2014;72:137-45. https://doi.org/10.1016/j.ejmech.2013.11.030
  11. Ha TJ, Lee MH, Kim HT, Kwon HS, Baek IY, Kubo I, Jang DS. Slow-binding inhibition of soybean lipoxygenase-1 by luteolin. Arch Pharm Res 2012;35:1811-6. https://doi.org/10.1007/s12272-012-1014-x
  12. Kim JP, Yun BS, Shim YK, Yoo ID. Inoscavin A, a new free radical scavenger from the mushroom Inonotus xeranticus. Tetrahedron Lett 1999;40:6643-4. https://doi.org/10.1016/S0040-4039(99)01406-9