Mycobiology

The Korean Society of Mycology (한국균학회)
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Lipoxygenase Inhibitory Activity of Korean Indigenous Mushroom Extracts and Isolation of an Active Compound from Phellinus baumii

  • Lee, Seung Woong (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Song, Ja-Gyeong (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Hwang, Byung Soon (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Kim, Dae-Won (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, Yoon-Ju (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Woo, E-Eum (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Kim, Ji-Yul (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Lee, In-Kyoung (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University) ;
  • Yun, Bong-Sik (Division of Biotechnology and Advanced Institute of Environment and Bioscience, Chonbuk National University)
  • Received : 2014.05.14
  • Accepted : 2014.05.20
  • Published : 2014.06.30
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Abstract

We investigated a total of 335 samples of Korean native mushroom extracts as part of our lipoxygenase (LOX) inhibitor screening program. Among the mushroom-methanolic extracts we investigated, 35 exhibited an inhibitory activity greater than 30% against LOX at a concentration of 100 ${\mu}g/mL$. Especially, Collybia maculata, Tylopilus neofelleus, Strobilomyces confusus, Phellinus gilvus, P. linteus, P. baumii, and Inonotus mikadoi exhibited relatively potent LOX inhibitory activities of 73.3%, 51.6%, 52.4%, 66.7%, 59.5%, 100.0%, and 85.2%, respectively. Bioassay-guided fractionation led to the isolation of inoscavin A from the methanolic extract of P. baumii, which showed the most potent activity and was identified by spectroscopic methods. Specifically, inoscavin A exhibited potent LOX inhibitory activity with an $IC_{50}$ value of $6.8{\mu}M$.

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References

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