• KSBB Journal
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• 제7권3호
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• pp.222-228
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• 1992

Thalictrum rugosum세포배양에서 세포증식 및 berberine생산에 미치는 자당 수용액의 영향을 연구하였다. 순수한 자당 수용액내에서는 무시할만한 세포증식에도 불구하고 상당한 berberine생성의 증가가 있었다. 자당의 농도가 높은 경우 세포의 수분 함유량이 매우 낮아졌으며 세포당 berberine생성량은 4배나 증가되었다. 최적 자당 농도는 8%로 확인되었다. 8%자당 수용액상에서의 회분배양 결과, 이 방법의 장점을 최대한 효과적으로 이용하기 위해서는 접종후 최소 5일 이상은 지나야 함을 알 수 있었다. 비타민, 생장조절제, 무기염류 등의 첨가는 생산성 향상에 도움이 되지 않았다.

• 한국응용과학기술학회지
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• 제9권1호
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• pp.1-6
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• 1992

New five N-acyl-N-methyl taurate and their sodium salts were synthesized by acylating sodium N-methyl taurate in water in the presense of inorganic alkali as a catalyst. Wherein acyl group is a straight-chain radical of 10 to 18 carbon atoms. These compounds were identified by infrared spectroscopy. Surface activities of these sodium N-acyl-N-methyl taurate including surface tension, Ross-Miles foaming powers, foam stabilities, emulsifying powers and detergency were measured respectively. Also critical micelle concentration(cmc) were evaluated. Consequently these anionic surfactants with long chain acyl amine showed good emulsifying power of O/W type and had a good detergence.

• 한국환경보건학회지
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• 제30권2호
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• pp.161-166
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• 2004

The impact of dissolved wastewater constituents on the treatment of synthetic bisphenol A (BPA) solutions was investigated under a variety of reaction conditions. The laccase enzyme from Trametes vesicolor was used for the BPA treatment. The constituents studied included various inorganic salts, organic compounds and heavy metal ions. BPA degradation was inhibited by sulfate, thiosulfate, sulfide, nitrite, and cyanide ions at 25 mg/$\ell$, 100mg/$\ell$, 25 mg/$\ell$ 150 mg/$\ell$, and 2.5 mg/$\ell$, respectively. However, the inhibitive effects of sulfide and sulfite on BPA degradation were diminished by additional aeration to oxidize them. Formaldehyde significantly reduced the rate of BPA degradation at 1.0% among organic compounds studied. Among heavy metal ions tested, Fe(II) substantially suppressed BPA removal at 1 mM. MgCl$_2$ and CaCl$_2$ exhibited great inhibition of BPA degradation at 25mM.

• Journal of Plant Biology
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• 제14권2호
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• pp.11-14
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• 1971

Zephyranthes candida, Narcissus pseudonarcissus and Crinum asiaticum pollen were placed near their pistil parts respectively on agar cultural media(microslides) containing 10% sucrose and 100mg/l botic acid plus 1% agar with or without calcium and some other calciumsupporting inorganic salts. If fresh pistils (100% moisture) were used pollen grew toward their pistil parts, showing "positive" tropism. This was also true when combinations among three different species were made. Pollen tubes grew away from the pistils if they were dried (below 10% moisture), showing "negative" tropism. Pollen could not show any tropic growth and thus grew at random of all directions if the pistil parts were incompletely dried (approximately 50% moisture). The similar tropic responses of pollen-tube growth with the three species could be demonstrated with etiehr wet or dried tooth-pick segments. Calcium jons in the basic medium merely promated pollen-tube growth and so either "positive" or "negative" tropism became rather distinctive, but they were not tropically active. Pollen tubes grow toward pistil parts with more moisture content and seem to be hydrotropically sensitive. This was assumed due to the cohesive force existing in water molecules.esive force existing in water molecules.

• Bulletin of the Korean Chemical Society
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• 제25권1호
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• pp.106-108
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• 2004

In this paper we have determined the thermodynamic parameters for the isomerization between the ${\alpha}$-cis and the ${\beta}$-cis isomers in vanadium(V)-propylenediaminetetraacetate complex in water by $^{51}V$ NMR spectroscopy. In addition, the effects of organic solvents (methanol, formamide and dimethylsulfoxide) and inorganic salts (NaCl, $NaClO_4\;and\;NH_4Cl$) on the isomerization in solution have been investigated.

• KSBB Journal
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• 제13권4호
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• pp.438-443
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• 1998

Effects of various environmental factors on cell size index(FCW/DCW) in Thalictrum rugosum. Lithospermum erythrorhizon and Taxus cuspidata plant cell suspension cultures were investigated. Time course change of cell size index were also observed. In batch cultures, FCW/DCW increased according to the decrease of sugar concentration. For short-term experiment within 24 hr, FCW/DCW value could be reduced significantly by increasing sugar concentration. When an osmoticum such as mannitol was added, FCW/DCW converged to a low value. Therefore, it was confirmed that osmolality of the medium was important in determining cell size or water content of the cells. Inorganic salts or treatment with organic solvent also exhibited some effect on the cell size index. However, pH and centrifugal force did not show any influences. On the other hand, it was found that the addition of Pluronic F-68 reduced FCW/DCW. By combining these results effectively, it may be possible to increase the cell concentration in high density culture to a higher extent.

• Bulletin of the Korean Chemical Society
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• 제12권4호
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• pp.411-413
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• 1991

The effects of electrolyte on the critical micelle concentration (cmc) and bromide counterion binding in the micelles of cetylpyridinium bromide (CPB) have been investigated by UV spectroscopy and conductance measurements. Salts used in this study decreased cmc in the order $Cl^-\;<\;Br^-\;<\;NO3^-$ (which parallels the lyotropic series for the inorganic anions) and the effects on cmc followed the equation proposed by Shinoda: log cmc = A - B log (cmc + [NaX]). In the equation, constant B represents the counterion binding to the micelles at cmc and for the micelle of CPB at $25^{\circ}C$, B=80.76%. The association constant for the binding of counterions to long chain cations within micelles was also derived from the cmc values and counterion binding constant to the micelles.

• Bulletin of the Korean Chemical Society
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• 제7권6호
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• pp.453-457
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• 1986

Stereochemical control of the Wittig reaction of the primary aldehyde, 8-acetoxyoctan-1-al (7) with the nonstabilized alkylide, triphenylphosphonium n-butylide (6), was achieved by controlling the reaction conditions including solvent, temperature and inorganic salts. These conditions can be applied to the direct sythesis of the mixture of (Z)-, and (E)-8-dodecen-1-yl acetate, the sex pheromone of the oriental fruit moth, Grapholitha molesta. The primary aldehyde, 8-acetoxyoctan-1-al (7) was synthesized from 1,8-octanediol which is cheap and readily available.

• Journal of Plant Biology
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• 제29권3호
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• pp.197-205
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• 1986

Leaf mesophyll protoplasts from five cultivars of tobacco (N. tabacum L.) were cultured. The protoplasts did not survive in culture medium containing Murashige and Skoog inorganic salts for over 6 days. NH4NO3 and FeSO4.Na2EDTA concentration of Murashige and Skoog medium were toxic in tobacco leaf mesophyll protoplast culture. Therefore we investigated optimum condition in Murashige and Skoog medium. High plating efficiency was obtained by reducing the concentrations of NH4NO3 and FeSO4.Na2EDTA to 1/3 and 1/10, respectively, on the supplemented with 5$\mu$M IAA, 0.5 $\mu$M 2.4-D 5 $\mu$M BAP. Plants were regenerated from protoplast-derived calluses.

• Archives of Pharmacal Research
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• 제13권1호
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• pp.28-32
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• 1990

Precipitation and purification of amylase secreted by Streptomyces aureofaciens 77 in liquid inorganic salts-starch medium under the optimum conditions were carried out. Ammonium sulphate fractionation was used to precipitate amylase in cell free culture filtrate. (NH/sub 4/)/sub 2/ SO/sub 4/ at a concentration of 50-70% saturation gave the highest enzyme yield. The obtained precipitates were redissolved in phosphate buffer (pH 7.0) and subjected to dialysis. The dialyzed enzyme preparation was applied to DEAE-cellulose column chromatography which resulted in an increase of purification up to 59.48 fold. A further step of purification was done by applying the obtained purified sample to Sephadex-G200 column chromatography which resulted in ann increase of purification up to 73. 92 fold. The results clearly indicated that the isolated amylase from S. aureofaciens 77 was only on type.