• 제목/요약/키워드: injury recovery

검색결과 613건 처리시간 0.029초

이등제통탕(二藤除痛湯)이 Taxol 처리 및 좌골신경의 압박 손상 후 유발된 랫드의 말초신경병증에 미치는 영향 (Effects of YideungJetong-Tang on Peripheral Neuropathy Induced by Taxol and Compression Injury in the Rat Sciatic Nerve)

  • 정호영;김철중;조충식
    • 대한한의학회지
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    • 제33권3호
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    • pp.133-146
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    • 2012
  • Background: Most antitumor agents have the side effect of chemotherapy-induced peripheral neuropathy (CIPN). Cancer patients who take antitumor agents suffer from CIPN, but there is no known treatment for it. Unlike the central nerve system, the peripheral nerve can self-repair, and the Schwann cell takes this mechanism. Objectives: In this study, we researched the effect of YideungJetong-Tang (YJT) extract on taxol-induced sciatic nerve damage, through in vitro and in vivo experiments. Also, we studied the effect of YJT extract on neurite recovery and anti-inflammatory effect after compression injury of sciatic nerve in vivo. Methods: Vehicle, taxol and taxol+YJT were respectively applied on sciatic nerve cells of rat in vitro, then the cells were cultured. The sciatic nerve cells and Schwann cells were then observed using Neurofilament 200, Hoechst, ${\beta}$ -tubulin, S-$100{\beta}$, caspase-3 and phospho-Erk1/2. CIPN was induced by taxol into the sciatic nerve of rat in vivo, then YJT extract was taken orally. The axons, Schwann cells and neurites of the DRG sensory nerve were then observed using Neurofilament 200, ${\beta}$-tubulin, Hoechst, S-$100{\beta}$, phospho-Erk1/2 and caspase-3. YJT was taken orally after sciatic nerve compression injury, and the changes in axon of the sciatic nerve, Schwann cells and TNF-${\alpha}$ concentration were observed. Results: The taxol and YJT treated group showed significant effects on Schwann cell recovery, neurite growth and recovery. In vivo, YJT compared with control group showed Schwann cell structural improvement and axons recovering effect after taxol-induced Schwann cell damage. After sciatic nerve compression injury, recovery of distal axon, changes of Schwann cell distribution, and anti-inflammatory response were observed in the YJT. Conclusions: Through this study, we found that after taxol-induced neurite damage of sciatic nerve in vivo and in vitro, YJT had significant effects on sciatic nerve growth and Schwann cell structural improvement. In vivo, YJT improved recovery of distal axons and Schwann cells and had an anti-inflammatory effect.

부자(附子)의 경구투여(經口投與)가 RAT의 척수손상(脊髓損傷)에 미치는 영향(影響) 및 비교(比較) (The Effects of Radix aconiti on the Contraction of Rat's Spinal Cord Injury)

  • 이건목;김경식;이강창
    • Journal of Acupuncture Research
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    • 제15권2호
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    • pp.105-116
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    • 1998
  • Purpose In this study, We observed the recovery process in the ability to move in the hind limbs of the rats whose spinal cord injuries were treated by Radix acouniti(RA). The purpose of this study is to see the effects of Radix acouniti(RA) water extract on the contraction of rat's spinal cord injury. Procedure First, the rats were subjected to hemisectional spinal cord injuries by a scalpel blade. Those rats, then, were divided into three groups: Sham operated rats group and the experimental group, which received the Radix acouniti(RA) water extract, and the control group, which had no treatment. Their recovery in the ability to walk was observed by the Open Field Test (OFT) for 14 days after the injuries. Method The OFT was applied at four points: the hip, knee, ankle joint, and the tail. Each joint was given a movement rating of from 0 to 3, depending on the amount of movement. A movement rating of 0 designates no movement, a 1 designates slight movement, a 2 designates increased movement, and a 3 designates active movement. Slight movement is defined as a joint displaying less than or equal to 30% of that joint range, increased movement is displaying less than or equal to 60% of that joint's range, and active movement is greater than or equal to 90% of that joint's range. Tail movement is also graded on a scale of 0 to 3. A rating of 0 indicates that the tail is down 100% of the time, one of 1 indicates that the tail is down more than 10%, one of 2 shows that the tail is down less than 50% but more than 10% of the time, and one of 3 shows that the tail is down less than 10% of the time. All four ratings were added together and then averaged to arrive at a single score. Results The sham group which did not go through spinal cord injuries showed near normal results on all 3 joints and tail from right after the operation, which one would expect. The RA oral application group showed more effective recovery of movememt function than the control group around 4 days after the spinal cord injuries. However, after 14 days, both groups displayed almost the same degree of movement recovery. The results of this study are summarized as follows: 1. After 14days the spinal cord injuries, movement was recovered in sham operated group, control group, and experimented group in the hip, the knee, the tail and then the ankle of rats, in that order. 2. Around 7 days after the spinal cord injuries, the experimental group proved the effectiveness of the therapy in terms of movement recovery. 3. The level of ALT, ALP, AST in RA treated group was slightly increased. 4. The level of BUN and creatinine in RA treated group was slightly increased. The above results indicate that RA therapy at an early stage can bring about better movement recovery in patients with spinal cord injuries from traffic accidents or industrial disasters. But there is apparent side effect of RA on clinical, therefore the study on this should be continued.

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좌골신경 손상 모델에서 보양환오탕 처리에 의한 축삭 재생반응성 분석 (Improved axonal regeneration by Boyanghwano-tang treatment in mice given sciatic nerve injury)

  • 장인애;김기중;남궁욱
    • 혜화의학회지
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    • 제25권1호
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    • pp.99-108
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    • 2016
  • While axons in the peripheral nerve can regenerate and lead to functional recovery to a certain extent after injury, its efficacy varies depending on the severity and duration of the injury. Here, we investigated the effects of Boyanghwano-tang (BYHOT) treatment on the regenerative responses in the sciatic nerves after prolonged transection and coaptation surgery. In mice given crush injury, axonal regeneration was completed when analyzed 1 week later and did not show any difference in regenerative reponses in the distal portion of the nerve between saline- and BYHOT-treated groups. In animal models with transection and reconnection, axonal regeneration was markedly retarded compared to animals with crush injury. Regenerating axons were extended into the reconnected distal portion of the nerve more actively in animals treated with BYHOT than saline controls. Cdc2 protein was similarly induced in nerves with crush injury and with transection and recollection, and its level was lower in BYHOT-treated animal than saline control when measured 2 weeks after nerve reconnection. These results suggest that BYHOT may be useful to promote axonal regeneration in the peripheral nerve after severe injury.

백서 하치조 신경 손상에 따른 감각 유발전위와 체성감각 유발전위의 변화에 관한 연구 (CHANGES OF SENSORY AND SOMATOSENSORY EVOKED POTENTIALS FOLLOWING A NEEDLE INJURY ON THE INFERIOR ALVEOLAR NERVE IN RATS)

  • 우승철;김수남;이동근;천상우
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제18권4호
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    • pp.652-672
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    • 1996
  • Dysfunction of the inferior alveolar nerve may result from trauma, diseases or iatrogenic injury. The development and refinement of an objective method to evaluate this clinical problem is highly desirable and needed, especially concerning for an increasing medico-legal issue. Evoked potential techniques have attracted considerable attention as a means of assessing the function and integrity of nerve pathways. The purpose of this study was to characterize the Sensory Evoked Potentials(SEPs) and Somatosensory Evoked Potentials(SSEPs) elicited by electrical stimulation of mental nerve. SEPs and SSEPs were measured and analyzed statistically before and after needle injury on the inferior alveolar nerve of Sprague-Dawalye rats. Measuring SEPs was more sensitive in evaluation of the recovery of sensory function from inferior alveolar nerve injury then measuring SSEPs but we measured SSEPs in the hope of providing a safe, simple and objective test to check oral and facial sensibility, which is acceptable to the patient. We stimulated mental nerve after needle injury on the inferior alveolar nerve and SEPS on the level of mandibular foramen and SSEPs on the level of cerebral cortex were recorded. Threshold, amplitude, and latency of both of SEPs and SSEPs were analyzed. The results were as follows ; 1. Threshold of SEPs and SSEPs were $184{\pm}14{\mu}A$ and $164{\pm}14{\mu}A$ respectively. 2 SEPs were composed of 2 waves, i.e., N1 N2 in which N1 was conducted by II fibers and N2 was conducted by III fibers. 3. SSEPS were composed of 5 waves, of which N1 and N2 shower statistically significant changes(p<0.01, unpaired t-test). 4. SEPs and SSEPs were observed to be abolished immediately after local anesthesia and recovered 30 minutes later. 5. SEPs were abolished immediately after injury. N1 of SSEPs was abolished immediately and amplitued of N2 was decreased($20.7{\pm}12.2%$) immediately after 23G needle injury, but N3, N4 and N5 did not change significantly. Recovery of waveform delayed 30 minutes in SEPs and 45 minutes in SSEPs. 6. The degree of decrease in amplitude of SEPs and SSEPs, after 30G needle injury was smaller than those with 23G. SEPs recorded on the level of mandibular foramen were though to be reliable and useful in the assessment of the function of the inferior alveolar nerve after injury. Amplitude of SSEPs reflected the function and integrity of nerve and measuring them provided a safe, simple and abjective test to check oral and facial sensibility. These results suggest that measuring SEPs and SSEPs are meaningful methods for objective assessment in the diagnosis of nerve injury. N1 and N2 of SSEPs can be useful parameters for the evaluation of the nerve function following a needle injury.

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Successful Management of a Comatose Patient with Traumatic Brain Exposure with a Fronto-Parieto-Occipital Flap

  • Maduba, Charles Chidiebele;Nnadozie, Ugochukwu Uzodimma
    • Journal of Trauma and Injury
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    • 제33권1호
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    • pp.48-52
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    • 2020
  • Composite skull defects in patients with severe head injuries are very challenging to manage. The dilemma when deciding whether to perform a definitive reconstruction is how long to wait for physiological recovery before an intervention complicates the situation. The inability of such patients to tolerate prolonged anesthetic exposure is a driving factor for performing the minimal intervention necessary to facilitate recovery. Herein, we present a case involving the successful immediate reconstructive treatment of a severely head-injured adolescent with a composite scalp defect secondary to trauma. A 14-year-old boy sustained a severe head injury from a motor vehicle accident with a composite scalp defect in the right fronto-parietal region. The frontal lobe was exposed, and the right eye was crushed and devitalized. The patient was deeply unconscious for 3 days, without any significant improvements before reconstructive surgery was proposed due to fear of possible meningitis resulting from the exposure of brain structures. We successfully managed the patient with a fronto-parieto-occipital flap, after which the patient promptly recovered consciousness.

Outcome Evaluation with Signal Activation of Functional MRI in Spinal Cord Injury

  • Jung, Jong-Kwon;Oh, Chang-Hyun;Yoon, Seung-Hwan;Ha, Yoon;Park, So-Ra;Choi, Byung-Hyune
    • Journal of Korean Neurosurgical Society
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    • 제50권3호
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    • pp.209-215
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    • 2011
  • Objective : The authors investigated the changes of cortical sensorimotor activity in functional MRI (fMRI) and functional recovery in spinal cord injury (SCI) patients who had been treated by bone marrow cell transplantation. Methods : Nineteen patients with SCI were included in this study; ten patients with clinical improvement and nine without. The cortical sensorimotor activations were studied using the proprioceptive stimulation during the fMRI. Results : Diagnostic accuracy of fMRI with neurological improvement was 70.0% and 44.4% for sensitivity and specificity, respectively. Signal activation in the ipsilateral motor cortex in fMRI was commonly observed in the clinically neurological improved group (p-value=0.002). Signal activation in the contralateral temporal lobe and basal ganglia was more commonly found in the neurological unimproved group (p-value<0.001). Signal activation in other locations was not statistically different. Conclusion : In patients with SCI, activation patterns of fMRI between patients with neurologic recovery and those without varied. Such plasticity should be considered in evaluating SCI interventions based on behavioral and neurological measurements.

Pulsed Electromagnetic Fields to Influence Pain and Muscle Healing Following Muscle Injury in Rats

  • Koo, Hyun-Mo;Na, Sang-Su;Yong, Min-Sik
    • Journal of Magnetics
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    • 제20권4호
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    • pp.377-380
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    • 2015
  • Muscle contusion has a negative effect on muscle function. Although several studies showed that pain control and muscle recovery is facilitated by pulsed electromagnetic fields (PEMF), there has not been much research regarding the specific effects of PEMF on them. The aim of the present study is to investigate effects of PEMF on pain and muscle recovery following extensor digitorum longus (EDL) contusion injury through measuring the expression of the c-fos proto-oncogene and nerve growth factor (NGF). Significantly reduced c-fos expression in the spinal cord was shown in PEMF groups compared with control (CON) groups. There was no significant difference between PEMF1 and CON1, but significantly increased NGF expression was shown in PEMF3 and PEMF5 compared with in CON groups, where the numbers in the group names are the days from contusion. In conclusion, PEMF could be used to not only reduce pain in muscle injuries by down-regulating c-fos expression in the spinal cord, but it could also influence muscle healing through increasing NGF expression in the injured muscle.

Effects of Sagunjatang-Ga-Nokyong on Neurologic Recovery in Rats after Spinal Cord Injury

  • Kim, Hyun-Seok;Yoon, Il-Ji
    • 대한한의학회지
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    • 제29권5호
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    • pp.1-13
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    • 2008
  • Objective : This study is investigate the effects of Sagunjatang-Ga-Nokyong(SGJ-NY) treatment on regenerative responses of corticospinal tract(CST) axons in the injured spinal cord. Methods :Using rats, we damaged their spinal cord, and then applied SGJ-NY extract to the lesion. Then we observed GAP-43 and NGF protein, astrcyte, axonal regeneration responses and axonal elongation. Result :Determination of GAP-43 and NGF protein levels were increased. And increased proliferation of astrocyte and enhanced processes in astrocytes were observed by SGJ-NY treatment. Higher number of astrocytes within the injury cavity in SGJ-NY treated group were showed, yet CSPG proteins were a weaker staining in the cavity in SGJ-NY. CST axons extended into the cavity and to the caudal area in SGJ-NY treated group were increased. Conclusion : SGJ-NY treatment might increase neural activity in the injured spinal cord tissue, and improved axonal regeneration responses. In this process, activation of astrocytes may play a role in promoting enhanced axonal elongation. the current study show that SGJ-NY exerts positive activity on inducing nerve regeneration responses by elevating neural tissue migration activities.

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중등도 외상성 뇌손상 흰쥐에서 천마약침(天麻藥鍼)이 운동조정 및 인지 기능회복에 미치는 효과 (Effects of Gastrodiae Elata Pharmacopuncture at GB20 on Motor Control and Cognitive Function in Mild TBI Rats)

  • 김경윤;정현우;김계엽
    • 동의생리병리학회지
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    • 제23권5호
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    • pp.1080-1086
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    • 2009
  • This study was designed to investigate the effects of Gastrodiae Elata Pharmacopuncure at GB20 on motor control and cognitive dysfunction recovery after mild traumatic brain injury in rats. Rats were divided into three groups; (1) no treatment after traumatic brain injury(experiment I), (2) Treatment with NPA after traumatic brain injury(experiment II), (3) Treatment with GEP after traumatic brain injury(experiment III). In our study, we carried out behavioral test(Rotarod, Morris water maze) and immunohistochemistry study of the change BDNF in the hippocampus(pre, $7^{th}$, $14^{th}$ day). In Rotarod test(motor control function) was significantly increased in the experimental group III as compared with experimental group I, II on $7^{th}$(p<0.01) and $14^{th}$ day(p<0.001). In Morris water maze test(cognitive function) was significantly decreased in the experimental group III as compared with experimental group I, II on $14^{th}$ day(p<0.001). In immunohistochemistric response of BDNF in the hippocampus, the experimental group III was more immune response than the other groups on $14^{th}$ day. These results imply that Gastrodiae Elata Pharmacopuncure at GB20 can play a role in facilitating recovery of motor control and cognitive function after mild traumatic brain injury in rats.

허혈성 뇌손상 모델 당뇨쥐의 복합운동프로그램의 효과 (The Effect of Complex Exercise Program of Diabetic Rats with Ischemic Brain Injury Model)

  • 김동현;방현수
    • 대한지역사회작업치료학회지
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    • 제6권1호
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    • pp.41-48
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    • 2016
  • 목적 : 본 연구의 목적은 허혈성 뇌손상시 회복의 지연을 초래하는 당뇨에 복합운동프로그램을 적용하였을 때, 신경학적 효과와 당뇨의 개선효과를 확인하고자 하였다. 연구방법 : 본 연구는 경상북도 소재의 동물실험 연구실에서 실시하였고 허혈성 뇌손상 모델의 흰쥐에게 STZ(streptozotocin)를 투여하고 당뇨를 유발한 흰쥐 10마리를 사용하였다. 흰쥐에게는 4주간 복합운동프로그램을 실시하여 뇌기능회복을 확인하기 위해 미로검사(maze test)를 실시하였고, 당뇨의 개선효과를 보기위해서 혈당을 확인하였다. 결과 : 허혈성 뇌손상 모델 당뇨쥐에게 복합운동프로그램을 적용한 결과, 뇌기능회복을 확인하기 위한 미로검사에서는 실패횟수(error)가 3주 후부터 유의하게 감소하였고(p<.05) 탈출시간은 1주 후부터 유의하게 감소하였다(p<.05). 그리고 당뇨의 개선효과를 확인하기 위한 혈당 검사에서는 대조군에서는 유의한 변화가 없었고 복합운동프로그램을 적용한 실험군에서는 4주 후부터 유의한 효과가 있음을 알 수 있었다(p<.05). 결론 : 뇌졸중, 당뇨 등의 성인, 노인성 질환이 증가되고 있는 현 시점에서 환자들에게 재활은 여명수명동안 삶의 질 개선에 매우 중요하다. 본 연구는 허혈성 뇌졸중과 당뇨를 모두 가지고 있는 동물모델에게 치료사가 쉽게 시행할 수 있는 복합운동프로그램 적용을 통하여 뇌기능회복과 당뇨 개선효과를 확인할 수 있었다.