• Natural Product Sciences
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• 제9권1호
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• pp.34-37
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• 2003

We examined methanol extracts prepared from the Korean plants for their inhibitory effects on rat lens aldose reductase (RLAR) activity in vitro. Among 41 plant extracts, the extracts of Lagerstroemia indica, Punica granatum, Eurya japonica, Liquidambar styraciflua, and Vitis coignetiae exhibited highest inhibitory potency, even more potent than tetramethylene glutaric acid (TMG), known as one of typical aldose reductase inhibitors $(IC_{50}\;value,\;0.51\;{\mu}g/ml)$. Especially, the extract of Lagerstroemia indica (Lythraceae), exhibited the most inhibitory potency $(IC_{50}\;value,\;0.069\;{\mu}/ml)$ on RLAR.

• 대한화학회지
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• 제37권1호
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• pp.68-75
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• 1993

분자구조를 기술하는 molecular connectivity index, Wiener index 및 ad hoc descriptor와 알코올, 에스테르, 케톤 화합물들이 생체내에서 나타내는 enzyme inhibitory potency, lipoxygenase inhibition, tadpole narcosis potency, 증기독성(vapor toxicity), 증발열(heat of vaporization)과 같은 성질들 사이의 상관관계를 조사하였다. 생물학적 활성의 종류에 따라 molecular descriptor 사이의 우열은 있으나, 대체로 좋은 상관관계식을 얻었다.

• 대한약침학회지
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• 제10권3호
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• pp.71-76
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• 2007

Objectives This experimental study was performed to investigate the continuous anti-bacterial potency of Coptidis rhizoma extract on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$ diluted Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$). Results 1. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was increased in propotion to increase volume on all samples except $20{\mu}l$. Anti-bacterial potency of Coptidis rhizoma extract on S. aureus was appeared continuous. 2. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared in 100%, 10% and was the same as anti-bacterial potency of 2 days and 6 days. Anti-bacterial potency with change of volume(100%) was increased in propotion to increase volume on all samples. Anti-bacterial potency with change of volume(10%) was appeared in $50{\mu}l$. Anti-bacterial potency of Coptidis rhizoma extract on S. epidermidis was appeared continuous. Conclusions Anti-bacterial potency of Coptidis rhizoma extract on cultivation of S. aureus & S. epidermidis was showed continuous.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.67-71
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• 2007

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Tangpo-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis)that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50 ${\mu}$l diluted Tangpo-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20 ${\mu}$l,30 ${\mu}$l,40 ${\mu}$l,50 ${\mu}$l). Results : 1. Anti-bacterial potency of Tanpo-san on S. aureus was not appeared all samples. Anti-bacterial potency with change of volume was increased in propotion to increase volume, and the Anti-bacterial potency of 2 days was equal to 6 days. Anti-bacterial potency of Tangpo-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Tangpo-san on S. epidermidis was appeared in 100%, 10% on 2 and 6 days, and the Anti-bacterial potency of 6 days was decreased. In 2 days, Anti-bacterial potency was appeared 40 and 50u1, in 6 days, Anti-bacterial potency was appeared all samples. Anti-bacterial potency with change of volume was increased in propotion to increase volume and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Tangpo-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Tangpo-san on cultivation of S. aureus showed continuous, but on cultivation of S. epidermidis was not showed continuous.

• 생약학회지
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• 제30권2호
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• pp.216-221
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• 1999

The NAD(P)-linked $3{\alpha}-Hydroxysteroid$ $dehydrogenase(3{\alpha}-HSD)$ of rat liver cytosol is powerfully inhibited by the non-steroidal anti-inflammatory drugs in rank-order of their therapeutic potency, and this observation has now been developed into a rapid screen for predicting the potency of products that show anti-inflammatory effect. Five-plants were screened by using this method. Among them, BuOH-fraction of Mucuna birdwoodiana showed strong inhibitory effect on $3{\alpha}-HSD$, and three isoflavone compounds were isolated. Inhibitory activates of isolated compounds were compared.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.87-91
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• 2007

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Jinpi-san on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Jinpi-san(100%, 10%, 1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Jinpi-san on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Jinpi-san on S. aureus was appeared continuous. 2. Anti-bacterial potency of Jinpi-san on S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in 100%, 10% on 6 days. In 100% Jinpi-san, Anti-bacterial potency of 6 days was increased, in 10%, Anti-bacterial potency of 2 days was increased, in 1%, Anti-bacterial potency of 6 days was disappear. Anti-bacterial potency with change of volume was increased in propotion to increase volume except for 20${\mu}$l of 6days and increased on 6 days, but bacteria was increased. Anti-bacterial potency of Jinpi-san on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Jinpi-san on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.19-23
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• 2007

Objectives : This experimental study was performed to investigate the continuous anti-bacterial potency of Sean-tang on cultivation of Staphylococcus species(S. aureus, S. epidermidis) that induce eye disease. Methods : Minimal inhibitory concentration(MIC) was measured by dropping to 50${\mu}$l diluted Sean-tang(100%, 10%,1%, 0.1%) on S. aureus, S. epidermidis that were cultivated from 2 to 6 days. 1. Anti-bacterial potency was measured by the size of inhibition zone with change of volume(20${\mu}$l, 30${\mu}$l, 40${\mu}$l, 50${\mu}$l). Results : 1. Anti-bacterial potency of Sean-tang on S. aureus was appeared in 100% and increased on 6 days. Anti-bacterial potency with change of volume was increased in propotion to increase volume. Anti-bacterial potency of Sean-tang on S. aureus was appeared continuous. 2. Anti-bacterial potency of S. epidermidis was appeared in 100%, 10%, 1% on 2 days and in100%, 10% on 6 days. In 100% Sean-tang, Anti-bacterial potency of 6 days was increased, in 10%, 1%, Anti-bacterial potency of 2 days was increased. Anti-bacterial potency with change of volume was increased inpropotion to increase volume and increased on 6 days, but bacteria was increased. Anti-bacterial potency Sean-tang on S. epidermidis wasn't appeared continuous. Conclusions : Anti-bacterial potency of Sean-tang on cultivation of S. aureus was showed continuous, but on cultivation of S. epidermidis was not showed continuous.

• Journal of Microbiology and Biotechnology
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• 제8권3호
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• pp.197-202
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• 1998

Aliphatic alkenals having 6 to 13 carbons were evaluated for antifungal activity against Saccharomyces cerevisiae. The activity was gradually increased with chain length, e.g., (E)-2-decenal and (E)-2-undecenal exhibited maximum potency, while (E)-2-dodecenal and (E)-2-tridecenal were completely inactive. Alkenals showed increasing inhibitory activity with chain length, as in the case of antifungal activity, towards glucose-induced medium acidification by the plasma membrane $H^+$-ATPase of S. cerevisiae. The group including (E)-2-nonenal, (E)-2-decenal, and (E)-2-undecenal exhibited maximum potency, but the potency of (E)-2-dodecenal and (E)-2-tridecenal demonstrated a sudden drop with respect to the former group. (E)-2-Nonenal revealed dose-responsive inhibition to the medium acidification and inhibited over 90% at a concentration of 1.25 mM ($175.3{\mu}g$/ml). In contrast to (E)-2-undecenal whose inhibitory efficiency increased with incubation time, inhibition by (E)-2-dodecenal was reversed with time. Of the tested alkenals, (E)-2-heptenal and (E)-2-octenal most highly inhibited ATP hydrolytic activity by the plasma membrane $H^+$ ATPase, while (E)-2-heptenal at 10 mM ($1121.8{\mu}g$/ml) showed an inhibitory efficacy of 93.2%.

• The Korean Journal of Physiology and Pharmacology
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• 제2권2호
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• pp.201-207
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• 1998

To evaluate the possibility that the ginseng saponins could be developed as an anti-arteriosclerotic agent, we examined the inhibitory effects of ginseng saponins (total saponin[TS], panaxatriol[PT], panaxadiol[PD]) on the expression of c-fos mRNA and the proliferation of cultured rat aortic vascular smooth muscle cells (VSMCs) stimulated by angiotensin II (Ang II). TS and PT (1.0 mg/ml) suppressed c-fos mRNA induction in VSMCs stimulated by $10^{-5}$ M Ang II. The order of inhibitory potency was PT>TS. Ginseng saponins ($0.01{\sim}1.0$ mg/ml) inhibited the proliferation of VSMCs stimulated by Ang II in a concentration dependent manner, the inhibitory potency was TS＞PT＞PD at $0.1{\sim}1.0$ mg/ml. These results suggest that ginseng saponins may suppress Ang II-stimulated proliferation of aortic VSMCs which can be seen in atherosclerosis, hypertension and restenosis.

• 대한약침학회지
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• 제10권3호
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• pp.47-52
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• 2007

Objective This experimental study was performed to investigate the effect of Coptidis rhizoma extract compared with quantity on Staphylococcus aureus that induce keratitis. Methods Minimal inhibitory concentration(MIC) was measured by dropping to $50{\mu}l$　according to density Coptidis rhizoma extract(100%, 10%, 1%, 0.1%) compared with quantity(40g, 80g, 160g). Anti-bacterial potency was measured by the size of inhibition zone with change of volume. Results 1. MIC on Staphylococcus aureus in Coptidis rhizoma extract was showed anti-bacterial potency compared with quantity and density in 100% and 10% of all samples(40g, 80g, 160g). 2. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. 3. MIC on Staphylococcus aureus in Coptidis rhizoma extract(40g, 80g, 160g) was showed anti-bacterial potency compared with quantity all samples ($20{\mu}l,\;30{\mu}l,\;40{\mu}l,\;50{\mu}l$) in 100% density. Anti-bacterial potency of 80g Coptidis rhizoma extract decreased compared with 40g. Anti-bacterial potency of 160g Coptidis rhizoma extract decreased compared with 40g in $20{\mu}l,\;30{\mu}l$. Conclusions Coptidis rhizoma extract was showed anti-bacterial potency compare with quantity and density. In herbal drug, antibacterial potency compare with quantity and density must be studied.