• Title/Summary/Keyword: inhibition ratio

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Antitumor flavonoids from Cephalotaxus koreana Nakai

  • Jin, Wen-Yil;Song, Gyu-Yong;Kim, Young-Ho;Lee, Young-Mi;Bae, Ki-Hwan
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.200.1-200.1
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    • 2003
  • Cephalotaxus koreana Nakai is an endemic species in Korea. The EtOH extract of leaf and branch from the plant showed potent antitumor activity in Teruhiro's method. The tumor volume inhibition ratio value is 25.2% with 20mg/kg in the BDF1 mouse injected LLC cell. We isolated one flavone, sciadopitysin (1), two flavone O-glycosides, quercetin 3-O-${\beta}$-D-glucuronide 6"-ethyl ester (2), apigenin 7-neohesperidoside (3) in comparison with literatures data. Compounds 1-3 showed stronger antitumor activity than Taxol used as positive control. The inhibition ratio values of compounds 1-3 is 34.9, 31.6, 34.0%, respectively, and Taxol is 27.0 % compared with control group.

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Inhibitory Effect of Ethanol Extract and Juice of the Korean Cherry (Prunus tomentosa Thunberg) on Tyrosinase Activity In vitro (앵두과즙과 Ethanol 추출액의 In vitro에서 Tyrosinase 활성 저해효과)

  • Hwang, Ho-Sun;Kim, Joong-Man;Song, Young-Ae;Jeon, Ye-Jung
    • Korean Journal of Food Science and Technology
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    • v.33 no.6
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    • pp.760-763
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    • 2001
  • To develop a functional beverage from Korean cherry (Prunus tomentosa Thunberg), inhibition effect of ethanol extract and juice of the korean cherry on melanin systhesis and tyrosinase activity in vitro was investigated. Inhibition ratio of tyrosinase activity increased as the concentration of solid of korean cherry juice increased, and inhibition affect was high in initiation step of enzyme reaction and then gradually decreased. Inhibition ratio of tyrosinase activity was high in the 70% (v/v) ethanol extract of the cherry and the highest in the ethyl acetate fraction of the 70% (v/v) ethanol extract. Ultimatly, the amounts of functional matter (melanin synthesis inhibitor) in the cherry was highest in ethyl acetate fraction of the ethanol extract.

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Antitumor Effect of Polysaccharide Produced from a Mutant of Acetobacter pasteurianus IFO 13751-5 (Acetobacter pasteurianus IFO 13751-5 변이주가 생산하는 다당류의 항암효과)

  • Kim, Dong-Seuk;Ryu, Beung-Ho
    • Korean Journal of Food Science and Technology
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    • v.23 no.4
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    • pp.405-409
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    • 1991
  • The polysaccharide was predominantly composed of glucose (79.60%), galactose (9.68%), mannose(8.67%), fructose (1.96%;) and xylose (0.09%). Major amino acid in protein was consisted of cystein, aspartic acid, valine and glutamic acid. The tumor growth inhibition ratio of the polysaccharide against Sarcoma 180 was at highest level of 64.96%, when administerd at the concentration of 50 mg/kg and life prologation ratio showed 28.91% at the concentration of 50 mg/kg. The direct cytotoxic effect of polysaccharide was not observed in the tumor bearing mice with Sarcoma 180 in vitro.

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Effect of Corrosion Inhibitor for Reinforcing Steel in Concrete Containing Chlorides (염화물을 함유한 콘크리트 중의 철근방식을 위한 방청제의 효과)

  • 문한영;김성수
    • Magazine of the Korea Concrete Institute
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    • v.10 no.6
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    • pp.325-333
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    • 1998
  • Under the seawater environment, the reinforced concrete structure is deteriorated due to physical and chemical attacks. The main deterioration mechanism is the chloride corrosion of reinforcing steel. The corrosion inhibitors have been used to protect the rebars from corrosion which are susceptible to chlorides in concrete. However, there is not clear conclusion about corrosion inhibitors yet. In this study, it is made the accelerated experiment with 3 kinds of corrosion inhibitors for various chloride ingresses. It is estimated corrosion inhibitors that inhibitors by Half-Cell Potential, corrosion area ratio and weight loss ratio. It is concluded that inhibitors are not effective to corrosion inhibition for excessive chloride ingress. However, the effect of inhibition is directly proportional ot contents of corrosion inhibitors in some chloride ingress.

Comparative study on avian influenza virus antibody titer by hemagglutination inhibition test and enzyme-linked immunosorbent assay in the mass zone layer (산란계 밀집지역에서 혈구응집억제반응과 효소면역측정법을 이용한 가금인플루엔자 혈중항체가의 비교 조사)

  • 이정원;엄성심;이성재;서이원;서석열;정동석;송희종
    • Korean Journal of Veterinary Service
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    • v.27 no.1
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    • pp.81-87
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    • 2004
  • This study was conducted to investigate the similarity between hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay(ELISA), the HI titer and mean ratio S/P ratio) of avian influenza virus. To perform this study, the 1,457 sera of layers 21 farms in May, July and September, respectively. As a result of HI test, positive rates were 480 to 422 (92.1%) in May, 494 to 394(79.8%) in July and 483 to 402(83.2%) in September, and the mean antibody titer were 4.6, 4.3, 4.0 to 0.3 decreased, respectively. The positive rates by ELISA, 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September, and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. The result of HI test and ELISA positive 480 to 422(92.1%), 475(99.0%), 494 to 394(79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, ELISA was shown more sensitive compare the HI titers.

Histopathological Study on Inhibition of Oogenesis by Quercetin in Japanese medaka (Oryzias latipes) (Japanese medaka에 있어 Quercetin의 난자성숙 저해에 대한 조직병리학적 연구)

  • 황갑수
    • Environmental Analysis Health and Toxicology
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    • v.14 no.1_2
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    • pp.55-63
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    • 1999
  • Endocrine disrupting chemicals probably cause the cytological or/and morphological changes of germinal cells in gonad. Accordingly, this study was aimed to make sure that the effect of hormone-mimicking chemicals on gonad morphology such as decrease of germinal cells, inhibition of cellular maturation and change in the ratio of germinal cells in the different developmental phase can be observed by histopathological procedures and can be a useful bio-indicator for the evaluation of endocrine disruption by environmental chemicals. In this experiment, female Japanese medaka were exposured to quercetin, a phytoestrogen, at the concentration of 100 $\mu\textrm{g}$/L. quercetin showed the significant decrease in the number and rate of vitellogenic follicular oocytes in the treated group for 4 and 6 weeks. The weak development of yolk could be also observed. We could conclude that quercetin has anti-estrogenic or androgen-like potency by exerting the inhibition effect on oogenesis in fish female- gonad. From the result of this study, the applied methods and techniques can be evaluated to be a useful biomonitoring means for water pollution, expecting a good result of the subsequent study on apoptosis.

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Self-Corrosion Protection of Polymer Cementitious Materials Using Terpolymer Powders with a Nitrite-type Hydrocalumite (아질산형 hydrocalumite와 터폴리머 분말수지를 병용한 폴리머 시멘트계 재료의 자기방청기능)

  • Hong, Sun-Hee;Kim, Wan-Ki
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2010.05a
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    • pp.73-76
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    • 2010
  • This study deals with the properties of polymer-modified mortars with a nitrite-type hydrocalumite, which are effectively used as intelligent patch materials for deteriorated reinforced concrete structures. The calumite is a material that can adsorb the chloride ions (Cl-) causing the corrosion of reinforcing bars and liberate the nitrite ions (NO2-) inhibiting the corrosion in reinforced concrete, and can provide a self-corrosion inhibition function to the reinforced concrete. Polymer-modified mortars using hydrocalumite and terpolymer powders are prepared with various calumite contents and polymer-binder ratios, and tested for corrosion inhibition. Subsequently, regardless of the polymer-binder ratio, the replacement of ordinary portland cement with the calumite has a marked effect on the corrosion-inhibiting property of the polymer-modified mortars.

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Development of Media as function of inhibition to algae growing up (조류생성 방지기능이 있는 담체 개발)

  • Lee, Won Ku;Lee, So Young;Park, Soo Young;Kim, Jin Han
    • Journal of Wetlands Research
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    • v.7 no.3
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    • pp.49-55
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    • 2005
  • In order to enhance pore ratio and durability, media-type algal bloom inhibition chemical was manufactured from powder form. The results showed that the media increased effective contact time between the water to be treated and the effective components in the chemical, and thus, chemical dose and sludge production were significantly reduced.

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Optimizing Carnosine Containing Extract Preparation from Chicken Breast for Anti-glycating agents

  • Kim, Seung-Ki;Kwon, Dodan;Kwon, Da-Ae;Paik, In Kee;Auh, Joong-Hyuck
    • Food Science of Animal Resources
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    • v.34 no.1
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    • pp.127-132
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    • 2014
  • Optimization of carnosine and anserine extraction from chicken breast was performed using response surface methodology (RSM) to obtain the maximized physiological activities for anti-glycation and anti-oxidation. The optimum extraction conditions were water extraction for 1.6 h in the case of the 20-wk laying hen muscle and water extraction for 2.12 h in the case of 90-wk laying hen muscle. Higher carnosine and anserine contents were measured in the 20-wk laying hen muscle, along with higher physiological activities, which increased in direct proportion with the dipeptide contents. The extracts prepared from the 20-wk laying hen under optimum conditions showed 57% inhibition of advanced glycated end-product formation, 64% inhibition of lipid peroxidation, and 61% of DPPH radical scavenging effects. On the other hand, 52% inhibition of AGE formation, 62% inhibition of lipid peroxidation, and 53% of DPPH radical scavenging effect were demonstrated within the 90-wk laying hen. In addition, the ratio of carnosine was a key indicator for the physiological activities of the extracts.

Separation and purification of substance having matrix metalloproteinase-9 inhibition effect in Ulmus davidiana Plancn. var. japonica Nakai (유근피(楡根皮)에 존재하는 matrix metalloproteinase-9 억제 물질의 분리 및 정제)

  • Han, Kee-Jung;Lee, Kwang-Soo;Kong, Kwang-Hoon;Cho, Sung-Hye
    • Analytical Science and Technology
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    • v.16 no.3
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    • pp.179-184
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    • 2003
  • Root bark of Ulmus davidiana Plancn. var. japonica Nakai was extracted by using several solvents with different polarities. Each extract was treated on the MMPs obtained from SK-Hep-1 in order to investigate inhibition effect. Zymography of MMPs showed that MeOH extract has significant inhibition effect. On the GC-MS analysis the highest mass to charge ratio (m/z) of the purified substance was 281. Also, on zymography of MMPs the substance showed 47% inhibition effect at the concentration of $314.7{\mu}g/g$. Cell viability of SK-Hep-1 was 60% at $31.47{\mu}g/g$.